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    • 64. 发明授权
    • Polynucleotides encoding human EPO-FC fusion proteins with prolonged half-life and enhanced erythropoietic activity in vivo
    • 编码具有延长的半衰期和增强体内红细胞生成活性的人EPO-FC融合蛋白的多核苷酸
    • US07964375B2
    • 2011-06-21
    • US12555742
    • 2009-09-08
    • Haitao WangDu YongRui ZhangJing XuLongbin Liu
    • Haitao WangDu YongRui ZhangJing XuLongbin Liu
    • A61K39/00C12P21/06C12N1/20C12N15/00C07K14/00C07H21/02
    • C07K14/505A61K9/0019A61K38/00A61K47/6811C07K2317/53C07K2319/30
    • A recombinant fusion protein comprising a human erythropoietin peptide portion linked to an immunoglobulin peptide portion is described. The fusion protein has a prolonged half-life in vivo in comparison to naturally occurring or recombinant native human erythropoietin. In one embodiment of the invention, the protein has a half-life in vivo at least three fold higher than native human erythropoietin. The fusion protein also exhibits enhanced erythropoietic bioactivity in comparison to native human erythropoietin. In one embodiment, the fusion protein comprises the complete peptide sequence of a human erythropoietin (EPO) molecule and the peptide sequence of an Fc fragment of human immunoglobulin IgG1. The Fc fragment in the fusion protein includes the hinge region, CH2 and CH3 domains of human immunoglobulin IgG1. The EPO molecule may be linked directly to the Fc fragment to avoid extraneous peptide linkers and lessen the risk of an immunogenic response when administered in vivo. In one embodiment the hinge region is a human Fc fragment variant having a non-cysteine residue at amino acid 6. The invention also relates to nucleic acid and amino acid sequences encoding the fusion protein and transfected cell lines and methods for producing the fusion protein. The invention further includes pharmaceutical compositions comprising the fusion protein and methods of using the fusion protein and/or the pharmaceutical compositions, for example to stimulate erythropoiesis in subjects in need of therapy.
    • 描述了包含与免疫球蛋白肽部分连接的人促红细胞生成素肽部分的重组融合蛋白。 与天然存在的或重组的天然人促红细胞生成素相比,融合蛋白在体内具有延长的半衰期。 在本发明的一个实施方案中,蛋白质的体内半衰期比天然人促红细胞生成素高至少三倍。 与天然人促红细胞生成素相比,融合蛋白也表现出增强的红细胞生物活性。 在一个实施方案中,融合蛋白包含人促红细胞生成素(EPO)分子的完整肽序列和人免疫球蛋白IgG1的Fc片段的肽序列。 融合蛋白中的Fc片段包括人免疫球蛋白IgG1的铰链区,CH2和CH3结构域。 EPO分子可以直接连接到Fc片段,以避免外来的肽接头并且当在体内施用时减轻免疫原性应答的风险。 在一个实施方案中,铰链区是在氨基酸6具有非半胱氨酸残基的人Fc片段变体。本发明还涉及编码融合蛋白和转染细胞系的核酸和氨基酸序列以及用于产生融合蛋白的方法。 本发明还包括包含融合蛋白的药物组合物和使用融合蛋白和/或药物组合物的方法,例如刺激需要治疗的受试者的红细胞生成。
    • 66. 发明申请
    • REDUCED BANDWIDTH OFF-LOADING OF ENTROPY CODING/DECODING
    • 减少带宽编码/解码的带宽偏移
    • US20100208825A1
    • 2010-08-19
    • US12370699
    • 2009-02-13
    • Jim Chen ChouRui Zhang
    • Jim Chen ChouRui Zhang
    • H04N7/26
    • H04N19/40H04N19/42H04N19/46H04N19/61
    • Techniques are provided herein to produce encoded video bitstreams and to similarly decode encoded video bitstreams according to a coding standard not supported by an on-chip encoder/decoder. For purposes of encoding, a video sequence is received at a first device. A first bitstream is generated at the first device by encoding the video sequence according to a first coding standard and information associated with the video sequence is generated at the first device according to a second coding standard. The first bitstream and the information are then transmitted to a second device. At the second device the first bitstream is decoded to produce a second bitstream. The second bitstream and the information are combined by removing syntax elements associated with the first coding standard from the second bitstream and adding the information to produce a third bitstream according to the second coding standard. Similar techniques are provided for decoding an encoded bitstream to recover a video sequence.
    • 本文提供了技术来产生编码视频比特流,并且根据不由片上编码器/解码器支持的编码标准类似地解码经编码的视频比特流。 为了编码的目的,在第一设备处接收视频序列。 通过根据第一编码标准编码视频序列,在第一设备处产生第一比特流,并且根据第二编码标准在第一设备处生成与视频序列相关联的信息。 然后将第一比特流和信息发送到第二设备。 在第二设备处,第一比特流被解码以产生第二比特流。 通过从第二比特流中除去与第一编码标准相关联的语法元素,并根据第二编码标准添加信息以产生第三比特流,来组合第二比特流和信息。 提供了用于解码编码比特流以恢复视频序列的类似技术。
    • 69. 发明申请
    • FUNCTIONAL POLYMER FOR ENHANCED OIL RECOVERY
    • 功能性聚合物用于增强油回收
    • US20100029880A1
    • 2010-02-04
    • US12429137
    • 2009-04-23
    • Rui ZhangYongchun Tang
    • Rui ZhangYongchun Tang
    • C08F228/02C08F214/16C08F226/02
    • C09K8/584
    • The present invention relates compositions and methods for enhanced oil recovery. The method is directed to employing a water-soluble The present invention relates compositions and methods for enhanced oil recovery (EOR). The method is directed to employing a water-soluble functional polymeric surfactant (FPS), with a medium IFT value, preferably ranged from about 0.1 to about 15 dyne/cm between water phase containing polymeric surfactant and hydrocarbon phase, for recovery of hydrocarbons from subterranean formations. The FPS solution demonstrates a strong interaction with oil and the great potential to increase both volumetric sweep efficiency and microscopic displacement efficiency in EOR.
    • 本发明涉及用于提高采油量的组合物和方法。 本方法涉及使用水溶性本发明涉及用于提高采油量(EOR)的组合物和方法。 该方法涉及使用具有中等IFT值的水溶性功能性聚合物表面活性剂(FPS),优选在含水聚合物表面活性剂和烃相之间约0.1至约15达因/厘米,用于从地下回收烃 地层。 FPS解决方案表现出与油的强烈相互作用,并且具有提高EOR中的体积扫描效率和微观位移效率的巨大潜力。