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61. 发明授权

US5320958A Isolated bacterial reverse transcriptase 失效
标题翻译：分离的细菌逆转录酶
公开(公告)号：US5320958A
公开(公告)日：1994-06-14
申请号：US315316
申请日：1989-02-24
申请人： Sumiko Inouye , Mei-Yin Hsu , Susan Eagle , Masayori Inouye
发明人： Sumiko Inouye , Mei-Yin Hsu , Susan Eagle , Masayori Inouye
IPC分类号： C07H21/04 , C07K14/00 , C07K14/195 , C07K14/41 , C12N9/12 , C12N15/09 , C12N15/54 , C12R1/01 , C12N1/00 , C12N1/12
CPC分类号： C12N9/1276 , Y10S435/822
摘要： The present invention relates to an isolated bacterial reverse transcriptase. The reverse transcriptase synthesizes a peculiar RNA-DNA complex called msDNA which is a single-stranded DNA structure branched out from an RNA molecule. The gene coding for the reverse transcriptase has been isolated and sequenced. It codes for a polypeptide of 485 amino acid residues. This is the first time that a reverse transcriptase has been found, identified and isolated from a prokaryotic microorganism of which the amino acid sequence is shown in FIGS. 2a-2d.
摘要翻译：本发明涉及分离的细菌逆转录酶。逆转录酶合成称为msDNA的特异性RNA-DNA复合物，其是从RNA分子分支的单链DNA结构。编码逆转录酶的基因已被分离并测序。它编码485个氨基酸残基的多肽。这是第一次从原核生物中发现，鉴定和分离了逆转录酶，其中氨基酸序列显示在图1和2中。 2a-2d。

62. 发明授权

US5190931A Regulation of gene expression by employing translational inhibition of MRNA utilizing interfering complementary MRNA 失效
公开(公告)号：US5190931A
公开(公告)日：1993-03-02
申请号：US436598
申请日：1989-11-15
申请人： Masayori Inouye
发明人： Masayori Inouye
IPC分类号： A61K38/00 , C07H21/00 , C12N15/113
CPC分类号： C12N15/113 , C07H21/00 , A61K38/00 , C12N2310/111
摘要： Gene expression in a cell can be regulated or inhibited by incorporating into or associating with the genetic material of the cell a non-native nucleic acid sequence which is transcribed to produce an mRNA which is complementary to and capable of binding to the mRNA produced by the genetic material of said cell.

63. 发明授权

US4863855A Novel cloning vehicles for polypeptide expression in microbial hosts 失效
标题翻译：用于微生物宿主中多肽表达的新型克隆载体
公开(公告)号：US4863855A
公开(公告)日：1989-09-05
申请号：US378481
申请日：1982-05-14
申请人： Masayori Inouye , Kenzo Nakamura , Yoshihiro Masui
发明人： Masayori Inouye , Kenzo Nakamura , Yoshihiro Masui
IPC分类号： C12N15/09 , C07H21/04 , C12N1/20 , C12N1/21 , C12N15/00 , C12N15/62 , C12N15/70 , C12P21/00
CPC分类号： C12N15/62 , C12N15/70
摘要： Methods and compositions are provided for regulated expression of polypeptides in transformed bacterial hosts. A novel class of plasmid cloning vehicles includes a DNA sequence coding for the desired polypeptide (or an insertion site therefor) linked for transcriptional expression in reading phase with one or more functional fragments derived from an outer membrane protein gene of a Gram-negative bacterium. The plasmids also include an inducible promoter sequence positioned in the proper orientation for transcriptional expression of the desired polypeptide, as well as a separate DNA sequence coding for a repressor molecule which can interact with the inducible promoter to prevent transcription therefrom. Expression of the desired polypeptide is under the control of both the constitutive promoter and the inducible promoter, although transcription from either promoter is normally blocked by the repressor molecule. However, the repressor can be selectively inactivated by means of an inducer molecule to permit transcriptional expression of the desired polypeptide from both promoters. The methods utilize such plasmids to introduce genetic capability into micro-organisms for the production of proteins, such as medically or commercially useful hormones, enzymes, immunogenic proteins, or intermediates therefor, but only in the presence of an appropriate inducer.
摘要翻译：提供了用于在转化的细菌宿主中调节多肽表达的方法和组合物。一类新颖的质粒克隆载体包括编码所需多肽（或其插入位点）的DNA序列，其与阅读阶段的转录表达连接，其与来自革兰氏阴性细菌的外膜蛋白基因的一种或多种功能片段连接。质粒还包括位于适当取向的诱导型启动子序列，用于所需多肽的转录表达，以及编码可与诱导型启动子相互作用以阻止其转录的阻遏物分子的单独DNA序列。所需多肽的表达受组成型启动子和诱导型启动子的控制，尽管来自任一启动子的转录通常被阻遏物分子阻断。然而，阻遏物可以通过诱导剂分子选择性地失活以允许来自两个启动子的所需多肽的转录表达。所述方法利用这种质粒将遗传能力引入微生物以产生蛋白质，例如医学或商业上有用的激素，酶，免疫原性蛋白质或其中间体，但仅在适当的诱导剂存在下。

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