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61. 发明专利

CA2325350C MICROBIAL STRAINS AND PROCESSES FOR THE MANUFACTURE OF BIOMATERIALS 未知
公开(公告)号：CA2325350C
公开(公告)日：2012-05-08
申请号：CA2325350
申请日：1999-03-30
申请人： METABOLIX INC
发明人： HUISMAN GJALT W , BOYNTON LAURA , HOROWITZ DANIEL M , GERNGROSS TILLMAN U , PEOPLES OLIVER P
IPC分类号： C12N1/21 , C12N15/09 , C12N1/08 , C12N9/22 , C12P7/62 , C12P19/00 , C12P19/04
摘要： DNA constructs and genetically engineered microbial strains constructed using these DNA constructs, which produce a nuclease enzyme with specificity for DNA and/or RNA, are provided. These strains secrete nuclease into the periplasm or growth medium in an amount effective to enhance productivity and/or recovery of polymer, and are particularly suited for use in high cell density fermentation processes. These constructs are useful for modifying microbial strains to improve production and recovery processes for polymers such as intracellular proteins, such as enzymes, growth factors, and cytokines; for producing polyhydroxyalkanoates; and for producing extracellular polysaccharides, such as xanthan gum, alginates, gellan gum, zooglan, hyaluronic acid and microbial cellulose.

62. 发明专利

AU2010295638A1 Generation of high polyhydroxybutrate producing oilseeds 未知
公开(公告)号：AU2010295638A1
公开(公告)日：2012-04-26
申请号：AU2010295638
申请日：2010-09-15
申请人： METABOLIX INC
发明人： PATTERSON NII , TANG JIHONG , HAN JIXIANG , TAVVA VENKATA , HERTIG ANDREW , ZHANG ZHIGANG , RAMSEIER THOMAS MARTIN , BOHMERT-TATAREV KAREN , PEOPLES OLIVER P , SNELL KRISTI D
IPC分类号： C12N15/82 , A01H5/00 , C12P7/62
摘要： Transgenic plants, plant material, plant cells, and genetic constructs for synthesis of biopolymers, for example polyhydroxyalkanoates ("PHA") are provided. In one embodiment, the transgenic plants synthesize polyhydroxybutyrate ("PHB"). In one embodiment the transgenic plant encodes siRNA for one or more of the genes encoding enzymes for producing PHA. In a more preferred embodiment, the siRNA expression is under the control of an inducible regulatory element. In another embodiment, the transgenic plant contains transgenes that encode expression enzymes that will degrade the polymer. In a preferred embodiment, the expression of these enzymes is under the control of a germination specific, inducible, or minimal promoter. In another embodiment, the transgenic plant contains transgenes encoding enzymes that increase carbon flow for polymer synthesis. In a preferred embodiment, these transgenes encode enzymes that increase carbon flow in the Calvin Cycle.

63. 发明专利

AU2010295637A1 Generation of high polyhydroxybutrate producing oilseeds 未知
公开(公告)号：AU2010295637A1
公开(公告)日：2012-04-26
申请号：AU2010295637
申请日：2010-09-15
申请人： METABOLIX INC , DONALD DANFORTH PLANT SCI CT
发明人： PATTERSON NII , TANG JIHONG , CAHOON EDGAR BENJAMIN , JAWORSKI JAN G , YANG WENYU , PEOPLES OLIVER P , SNELL KRISTI D
IPC分类号： A01H1/02 , C12N15/82
摘要： Transgenic oilseed plants, plant material, plant cells, and genetic constructs for synthesis of polyhydroxyalkanoates ("PHA") are provided. In a preferred embodiment, the transgenic oilseed plants synthesize (poly)3- hydroxybutyrate ("PHB") in the seed. Genes utilized include phaA,phaB, phaC, all of which are known in the art. The genes can be introduced in the plant, plant tissue, or plant cell using conventional plant molecular biology techniques.

64. 发明专利

CA2773703A1 GENERATION OF HIGH POLYHYDROXYBUTRATE PRODUCING OILSEEDS 未知
公开(公告)号：CA2773703A1
公开(公告)日：2011-03-24
申请号：CA2773703
申请日：2010-09-15
申请人： METABOLIX INC , DONALD DANFORTH PLANT SCI CT
发明人： PATTERSON NII , TANG JIHONG , CAHOON EDGAR BENJAMIN , JAWORSKI JAN G , YANG WENYU , PEOPLES OLIVER P , SNELL KRISTI D
IPC分类号： A01H1/02 , C12N15/82
摘要： Transgenic oilseed plants, plant material, plant cells, and genetic constructs for synthesis of polyhydroxyalkanoates ("PHA") are provided. In a preferred embodiment, the transgenic oilseed plants synthesize (poly)3- hydroxybutyrate ("PHB") in the seed. Genes utilized include phaA,phaB, phaC, all of which are known in the art. The genes can be introduced in the plant, plant tissue, or plant cell using conventional plant molecular biology techniques.

65. 发明专利

CA2754261A1 STABLE, FERTILE, HIGH POLYHYDROXYALKANOATE PRODUCING PLANTS AND METHODS OF PRODUCING THEM 未知
公开(公告)号：CA2754261A1
公开(公告)日：2010-09-10
申请号：CA2754261
申请日：2010-03-05
申请人： METABOLIX INC
发明人： BOHMERT-TATAREV KAREN , MCAVOY SUSAN , PEOPLES OLIVER P , SNELL KRISTI D
IPC分类号： C12N15/82
摘要： Transgenic plants that produce high levels of polyhydroxybutyrate and methods of producing them are provided. In a preferred embodiment the transgenic plants are produced using plastid transformation technologies and utilize genes which are codon optimized. Stably transformed plants able to produce greater than 10% dwt PHB in tissues are also provided.

66. 发明专利

CA2322729C MODIFICATION OF FATTY ACID METABOLISM IN PLANTS 未知
公开(公告)号：CA2322729C
公开(公告)日：2010-06-15
申请号：CA2322729
申请日：1999-03-05
申请人： METABOLIX INC
发明人： PEOPLES OLIVER P , BOYNTON LAURA , HUISMAN GJALT W , MOLONEY MAURICE M , PATTERSON NII , SNELL KRISTI
IPC分类号： A01H5/00 , C12N15/52 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/00 , C12N15/09 , C12N15/82
摘要： Methods and systems to modify fatty acid biosynthesis and oxidation in plants to make new polymers are provided. Two enzymes are essential: a hydratase such as D-specific enoyl-CoA hydratase, for example, the hydratase obtained from Aeromonas caviae, and a .beta.-oxidation enzyme system. Some plants have a .beta.-oxidation enzyme system which is sufficient to modify polymer synthesis when the plants are engineered to express the hydratase. Examples demonstrate production of polymer by expression of these enzymes in transgenic plants. Examples also demonstrate that modifications in fatty acid biosynthesis can be used to alter plant phenotypes, decreasing or eliminating seed production and increasing green plant biomass, as well as producing polyhydroxyalkanoates.

67. 发明专利

DE69938105T2 未知
公开(公告)号：DE69938105T2
公开(公告)日：2009-01-29
申请号：DE69938105
申请日：1999-08-04
申请人： METABOLIX INC
发明人： SKRALY FRANK , PEOPLES OLIVER P
IPC分类号： A01H1/00 , C12P7/42 , C08G8/04 , C08G8/26 , C12N1/21 , C12N15/00 , C12N15/09 , C12N15/52 , C12N15/82 , C12P7/62 , C12R1/19
摘要： Organisms arc provided which express enzymes such as glycerol dehydratase, diol dehydratase, acyl-CoA transferase, acyl-CoA synthetase ²-ketothiolase, a;xtoaeetyl-CoA reductase, PHA synthase, glycerol-3-phosphate dehydrogenase and glycerol-3-pbospharase, which are useful for the production of PHAs. In some cases one or more of these genes are native to the host organism and the remainder are provided from transgenes. These organisms produce poJy(3-hydroxyalkanoare) homopolymers or co-polymers incorporating 3-hydroxypropionate or 3-hydroxyvalerate monomers wherein the 3-hydroxynropionate and 3-hydroxyvalerate units are derived from the enzyme catalysed conversion of diols. Suitable diols that can be used include 1,2-propanediol, 1,3-propanediol and glycerol. Biochemical pathways for obtaining the glycerol from normal cellular metabolites are also described. The PHA polymers are readily recovered and industrially useful as polymers or as starting materials for a range of chemical intermediates including 1,3-propanediol, 3-hydroxypropionaldehyde, -acrylics, malonic acid, esters and amines.

68. 发明专利

DE69838768D1 未知
公开(公告)号：DE69838768D1
公开(公告)日：2008-01-03
申请号：DE69838768
申请日：1998-09-18
申请人： METABOLIX INC
发明人： HUISMAN GJALT W , SKRALY FRANK A , MARTIN DAVID P , PEOPLES OLIVER P
IPC分类号： C12N9/10 , C12N15/09 , C08G63/06 , C12N1/21 , C12N9/02 , C12N15/53 , C12N15/54 , C12N15/70 , C12N15/82 , C12P1/04 , C12P7/00 , C12P7/52 , C12P7/62 , C12R1/19
摘要： The gene encoding a 4-hydroxybutyryl-Co A transferase has been isolated from bacteria and integrated into the genome of bacteria also expressing a polyhydroxyalkanoate synthase, to yield an improved production process for 4HB-containing polyhydroxyalkanoates using transgenic organisms, including both bacteria and plants. The new pathways provide means for producing 4HB containing PHAs from cheap carbon sources such as sugars and fatty acids, in high yields, which are stable. Useful strains are obtaining by screening strains having integrated into their genomes a gene encoding a 4HB-CoA transferase and/or PHA synthase, for polymer production. Processes for polymer production use recombinant systems that can utilize cheap substrates. Systems are provided which can utilize amino acid degradation pathways, α-ketoglutarate, or succinate as substrate.

69. 发明专利

CA2339351C POLYHYDROXYALKANOATE PRODUCTION FROM POLYOLS 未知
公开(公告)号：CA2339351C
公开(公告)日：2007-07-03
申请号：CA2339351
申请日：1999-08-04
申请人： METABOLIX INC
发明人： SKRALY FRANK , PEOPLES OLIVER P
IPC分类号： A01H1/00 , C12P7/42 , C08G8/04 , C08G8/26 , C12N1/21 , C12N15/00 , C12N15/09 , C12N15/52 , C12N15/82 , C12P7/62 , C12R1/19
摘要： Organisms are provided which express enzymes such as glycerol dehydratase, diol dehydratase, acyl-CoA transferase, acyl-CoA synthetase .beta.-ketothiolase, acetoacetyl-CoA reductase, PHA synthase, glycerol-3-phosphate dehydrogenase and glycerol-3-phosphatase, which are useful for the production of PHAs. In some cases one or more of these genes are native to the host organism and the remainder are provided from transgenes. These organisms produce poly(3- hydroxyalkanoate) homopolymers or co-polymers incorporating 3-hydroxypropionate or 3-hydroxyvalerate monomers wherein the 3- hydroxypropionate and 3-hydroxyvalreate units are derived from the enzyme catalysed conversion of diols. Suitable diols that can be used include 1,2-propanediol, 1,3-propanediol and glycerol. Biochemical pathways for obtaining the glycerol from normal cellular metabolites are also described. The PHA polymers are readily recovered and industrially useful as polymers or as starting materials for a range of chemical intermediates including 1,3-propanediol, 3-hydroxypropionaldehyde, acrylics, malonic acid, esters and amines.

70. 发明专利

AT323152T 未知
公开(公告)号：AT323152T
公开(公告)日：2006-04-15
申请号：AT98948384
申请日：1998-09-18
申请人： METABOLIX INC
发明人： HUISMAN GJALT W , SKRALY FRANK A , MARTIN DAVID P , PEOPLES OLIVER P
IPC分类号： C12N15/09 , C08G63/06 , C12N1/21 , C12N9/02 , C12N9/10 , C12N15/53 , C12N15/54 , C12N15/82 , C12P7/52 , C12P7/62 , C12R1/19 , C12N15/70 , C12P1/04 , C12P7/00
摘要： The gene encoding a 4-hydroxybutyryl-Co A transferase has been isolated from bacteria and integrated into the genome of bacteria also expressing a polyhydroxyalkanoate synthase, to yield an improved production process for 4HB-containing polyhydroxyalkanoates using transgenic organisms, including both bacteria and plants. The new pathways provide means for producing 4HB containing PHAs from cheap carbon sources such as sugars and fatty acids, in high yields, which are stable. Useful strains are obtaining by screening strains having integrated into their genomes a gene encoding a 4HB-CoA transferase and/or PHA synthase, for polymer production. Processes for polymer production use recombinant systems that can utilize cheap substrates. Systems are provided which can utilize amino acid degradation pathways, α-ketoglutarate, or succinate as substrate.

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