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    • 68. 发明公开
    • COMPOSITION FOR HOT-START REVERSE TRANSCRIPTION REACTION OR HOT-START REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION
    • ZUSAMMENSETZUNGFÜREINE热启动反向转换器漏电器EINE热启动反向转换保护膜消隐功能
    • EP2824189A1
    • 2015-01-14
    • EP13757504.9
    • 2013-03-11
    • Bioneer Corporation
    • PARK, Han OhLEE, Jun HeeCHOI, SoraKIM, Hyun Seo
    • C12Q1/42C12Q1/68C12Q1/48
    • C12Q1/6886C12P19/34C12Q1/42C12Q1/48C12Q1/6848C12Q1/686C12Q2521/107C12Q2527/125C12Q2549/101
    • The present invention relates to a composition for hot-start reverse transcription reaction and a composition for reverse transcription PCR, which comprises the same, and more particularly to a reaction composition obtained by adding pyrophosphate and pyrophosphatase to an aqueous solution containing reaction buffer solution, MgCl 2 , four kinds of dNTPs, and reverse transcription polymerase in a single reaction tube, and a composition for hot-start reverse transcription reaction obtained by freezing or drying the reaction composition and having increased stability and long- term storage stability. Also, the present invention relates to a composition that comprises DNA polymerase in the composition for hot-start reverse transcription reaction, and thus enables a hot-start reverse transcription reaction and a PCR reaction to be sequentially performed, and to a method for amplifying nucleic acid the same. In comparison with conventional compositions for reverse transcription reaction, the composition for hot-start reverse transcription reaction can prevent non-specific reverse transcription reactions during mixing at room temperature and can selectively perform the reverse transcription of the target RNA at the reaction temperature, making it possible to amplify the reverse transcription product by PCR with high sensitivity. Thus, the composition of the invention can be conveniently and effectively used in multiplex reverse transcription PCRs or real-time quantitative reverse transcription PCR.
    • 本发明涉及一种热启动逆转录反应用组合物和逆转录PCR用组合物,更具体地说,涉及通过向含有反应缓冲液的水溶液中添加焦磷酸盐和焦磷酸酶而获得的反应组合物,MgCl 2,单个反应管中的四种dNTP和逆转录聚合酶,以及通过冷冻或干燥反应组合物并具有增加的稳定性和长期储存稳定性而获得的用于热启动逆转录反应的组合物。 此外,本发明涉及在热启动逆转录反应的组合物中含有DNA聚合酶的组合物,因此能够依次进行热启动逆转录反应和PCR反应,以及用于扩增核酸的方法 酸也一样。 与用于逆转录反应的常规组合物相比,用于热启动逆转录反应的组合物可以在室温下混合期间防止非特异性逆转录反应,并且可以在反应温度下选择性地进行靶RNA的逆转录,使其 可能通过PCR以高灵敏度扩增逆转录产物。 因此,本发明的组合物可以方便有效地用于多重逆转录PCR或实时定量逆转录PCR。