会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 66. 发明授权
    • Two-photon microscopy with plane wave illumination
    • 双光子显微镜与平面波照明
    • US6020591A
    • 2000-02-01
    • US892843
    • 1997-07-11
    • Donald J. HarterJeff A. Squier
    • Donald J. HarterJeff A. Squier
    • G02B21/02G02B21/00G01N21/64
    • G02B21/0084G02B21/0076
    • A two-photon fluorescence microscope employs two laser beams having pulses of respective wavelengths .lambda..sub.2 and .lambda..sub.3, which cause two-photon emission of a fluorophore when the pulses are spatially and temporally overlapping. The pulses of the two beams of wavelengths .lambda..sub.2 and .lambda..sub.3 are combined at some crossing angle .theta. within the specimen, causing two-photon absorption within a line-shaped region during each instant of overlap. As the pulses pass through each other, the overlapping line-shaped region moves such that a slice of the fluorophore-containing specimen is excited by two-photon absorption during the overlap period. Lateral scanning is effected without moving parts by adjusting the relative delay of the pulses in the two beams. When the crossing angle .theta. is set to 0, i.e., when the two beams are directed along the same axis, the pulses of the two beams form a pancake-shaped volume in which two-photon excitation occurs as the pulses spatially and temporally overlap while traveling in opposite directions. A two-dimensional detector, such as a two-dimensional charge coupled device (CCD) array, can be used to detect a two-dimensional portion of the specimen at one time without lateral scanning. A three-dimensional image can be produced by adjusting the time delay between the two pulses, thereby changing the location of the "pancake" volume created by the intersection of the two pulses.
    • 双光子荧光显微镜使用具有各自波长λ2和λ3的脉冲的两个激光束,当脉冲在空间上和时间上重叠时,其产生荧光团的双光子发射。 波长λ2和λ3的两个波长的脉冲在样本内的某个交叉角度θ处组合,在每个重叠时刻内在线状区域内引起双光子吸收。 当脉冲彼此通过时,重叠的线状区域移动,使得在重叠期间,含荧光团的样品的切片被双光子吸收激发。 通过调整两个光束中的脉冲的相对延迟来实现横向扫描而不移动部分。 当交叉角θ设置为0时,即当两个光束沿着相同的轴线被引导时,两个光束的脉冲形成薄饼状体积,其中双光子激发发生在脉冲空间和时间上重叠的同时 沿相反方向行驶。 诸如二维电荷耦合器件(CCD)阵列之类的二维检测器可用于一次检测样本的二维部分而不进行横向扫描。 可以通过调整两个脉冲之间的时间延迟来产生三维图像,从而改变由两个脉冲的交点产生的“煎饼”体积的位置。