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51. 发明专利

JP2009039129A Mutant luciferase 有权
标题翻译： MUTANT LUCIFERASE
公开(公告)号：JP2009039129A
公开(公告)日：2009-02-26
申请号：JP2008244507
申请日：2008-09-24
申请人： Promega Corp , プロメガ・コーポレイシヨン
发明人： WOOD KEITH V , GRUBER MONIKA G
IPC分类号： C12N15/09 , C12N9/02 , C12N15/53 , C12P21/02
CPC分类号： C12N9/0069 , C12Y113/12007
摘要： PROBLEM TO BE SOLVED: To provide a non-natural active mutant of a beetle luciferase, and a DNA encoding such a mutant. SOLUTION: This mutant luciferase is obtained from beetles. The mutant luciferase is different from a corresponding wild type luciferase by a fact that it generates a biological luminescence having peak intensity at a wavelength different by at least 1 nm from the peak intensity of the biological luminescence generated from the wild type enzyme. The mutant luciferase and DNA can be utilized for various biological detections. COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：待解决的问题：提供甲虫荧光素酶的非天然活性突变体和编码这种突变体的DNA。解决方案：该突变荧光素酶由甲虫获得。突变荧光素酶与相应的野生型萤光素酶不同，其生成的生物发光具有与从野生型酶产生的生物发光的峰强度不同至少1nm的波长的峰强度。突变荧光素酶和DNA可用于各种生物检测。版权所有（C）2009，JPO＆INPIT

52. 发明专利

JP2007006910A Synthetic nucleic acid molecule compositions and methods of preparation 审中-公开
标题翻译：合成核酸分子组合物及其制备方法
公开(公告)号：JP2007006910A
公开(公告)日：2007-01-18
申请号：JP2006288147
申请日：2006-10-23
申请人： Promega Corp , プロメガ・コーポレーション
发明人： WOOD KEITH V , WOOD MONIKA G , ZHUANG YAO , PAGUIO AILEEN
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/02 , C12N15/67
CPC分类号： C12N9/0069 , C12N15/67
摘要： PROBLEM TO BE SOLVED: To provide synthetic nucleic acid molecules having altered codon usage without introduction of inappropriate or unintended transcription regulatory sequence for expression in a particular host cell. SOLUTION: A synthetic nucleic acid molecule is provided which comprises at least 300 nucleotides of a coding region for a polypeptide, has a codon composition differing at more than 25% of the codons from a wild type nucleic acid sequence encoding a polypeptide, and has at least 3-fold fewer transcription regulatory sequences than the average number of sequence which would result if the differing codons were randomly selected, wherein the transcriptional regulatory sequence is selected from the group consisting of transcription factor binding sequences, intron splice sites, poly (A) addition sites and promoter sequences, and wherein the polypeptide encoded by the synthetic nucleic acid molecule has at least 85% of sequence identity to the polypeptide encoded by the wild type nucleic acid sequence. COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：要解决的问题：提供具有改变的密码子使用的合成核酸分子，而不引入用于在特定宿主细胞中表达的不适当或非预期的转录调节序列。解决方案：提供包含多肽编码区的至少300个核苷酸的合成核酸分子，其密码子组成不同于编码多肽的野生型核酸序列的密码子的25％以上，并且具有比如果随机选择不同密码子时将导致的平均序列数少至少3倍的转录调节序列，其中转录调控序列选自转录因子结合序列，内含子剪接位点，聚（A）加成位点和启动子序列，并且其中由合成核酸分子编码的多肽与野生型核酸序列编码的多肽具有至少85％的序列同一性。版权所有（C）2007，JPO＆INPIT

53. 发明专利

JP2001224398A LUCIFERASE COMPOSITIONS AND METHODS 失效
公开(公告)号：JP2001224398A
公开(公告)日：2001-08-21
申请号：JP2000400335
申请日：2000-12-28
申请人： PROMEGA CORP
发明人： WOOD KEITH V
IPC分类号： G01N21/76 , C12N9/02 , C12Q1/66
摘要： PROBLEM TO BE SOLVED: To provide an improved method for analyzing photogeneration speed derived from luciferase activity in a beetle luciferase-luciferin reaction and to obtain a composition therefor. SOLUTION: A method, a composition and an assaying kit for assaying samples containing the beetle luciferase or ATP(adenosine triphosphate) by using the beetle luciferase-luciferin reaction are provided. Furthermore, coenzyme A, a conjugation of the beetle luciferase and oxyluciferin which is an excited state of the luciferase-luciferin reaction and a thioester of the coenzyme A and a luciferin [D-(-)-2-(6'-hydroxy-2'-benzothiazolyl)-thiazoline-4-carboxylic acid] are also provided. The figure 4 denotes luminescent curve at the time nothing is added in a normal assay when the CoA is present independently in the assay. The total photogenerated amount of >=15 times can be observed when the CoA, DTT(dithiothreitol) and BSA(bovine serum albumin) are added as compared when nothing is added.

54. 发明公开

EP1812602A4 METHODS AND KITS FOR DETECTING MUTATIONS 审中-公开
标题翻译：方法和药盒突变的检测
公开(公告)号：EP1812602A4
公开(公告)日：2008-06-11
申请号：EP05819475
申请日：2005-10-24
申请人： PROMEGA CORP
发明人： BACHER JEFFERY , HALBERG RICHARD , KENT-FIRST MARIJO , WOOD KEITH V
IPC分类号： C12Q1/68 , C07H21/02 , C07H21/04 , C12N1/20 , C12N5/06 , C12N15/74
CPC分类号： C12Q1/6879 , C12Q1/6858 , C12Q1/6886 , C12Q1/6888 , C12Q1/6897 , C12Q2600/136 , C12Q2600/16 , C12Q2525/151

55. 发明公开

EP1546162A4 LUMINESCENCE-BASED METHODS AND PROBES FOR MEASURING CYTOCHROME P450 ACTIVITY 有权
标题翻译：对测量细胞色素P450AKTIVITÜT发光的基础的方法和探针
公开(公告)号：EP1546162A4
公开(公告)日：2007-07-18
申请号：EP03749715
申请日：2003-09-16
申请人： PROMEGA CORP
发明人： CALI JAMES J , KLAUBERT DIETER , DAILY WILLIAM , HO SAMUEL KIN SANG , FRACKMAN SUSAN , HAWKINS ERIKA , WOOD KEITH V
IPC分类号： C07D403/04 , C07D413/04 , C07D417/04 , C07D417/14 , C07F9/06 , C12N9/02 , C12Q1/00 , C12Q1/26 , C12Q1/66 , G01N20060101 , G01N33/53 , G01N33/58
CPC分类号： G01N33/502 , A61K49/0008 , C07D417/04 , C12Q1/66 , G01N33/5088 , G01N33/582 , G01N2333/90245 , G01N2333/90251 , G01N2500/10
摘要： The invention provides compounds, compositions, methods, substrates, and kits useful for analyzing the metabolic activity in cells, tissue, and animals and for screening test compounds for their effect on cytochrome P450 activity. In particular, a one-step and two-step methods using luminogenic molecules, e.g. luciferins or coelenterazines, that are cytochrome P450 substrates and that are also bioluminescent enzyme, e.g., luciferase, pro-substrates are provided. The present method further provides a method for stabilizing and prolonging the luminescent signal in a luciferase-based assay using luciferase stabilizing agents such as reversible luciferase inhibitors.

56. 发明公开

EP1558729A4 RAPIDLY DEGRADED REPORTER FUSION PROTEINS 有权
标题翻译： FAST开采报导融合蛋白
公开(公告)号：EP1558729A4
公开(公告)日：2006-09-06
申请号：EP03754599
申请日：2003-09-16
申请人： PROMEGA CORP
发明人： ZDANOVSKY ALEXEY , ZDANOVSKAIA MARINA , MA DONGPING , WOOD KEITH V , ALMOND BRIAN D , WOOD MONIKA G
IPC分类号： C12N15/09 , C07H21/04 , C07K19/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/02 , C12N9/06 , C12N9/10 , C12N9/22 , C12N9/24 , C12N9/38 , C12P21/04 , C12Q1/02 , C12Q1/68 , C12N9/14 , A61K38/44 , C07K1/00 , C12N1/20 , C12N9/62 , C12N15/00 , C12P21/06 , C12Q1/00 , C12Q1/34
CPC分类号： C12N15/67 , C07K2319/60 , C07K2319/61 , C07K2319/95 , C12N15/63 , C12Q1/6876 , C12Q1/6897

57. 发明公开

EP1815226A4 DEVICE AND METHOD FOR PURIFICATION OF BIOLOGICAL MATERIALS 审中-公开
标题翻译： DEVICE AND METHOD FOR清洁生物材料
公开(公告)号：EP1815226A4
公开(公告)日：2008-03-19
申请号：EP05851531
申请日：2005-11-09
申请人： PROMEGA CORP
发明人： EKENBERG STEVEN J , WOOD KEITH V , ENGEL LAURIE
IPC分类号： G01N1/00 , B01L3/00 , B01L99/00 , C07H21/00 , C07H21/04 , C12M1/00 , C12P19/34 , C12Q1/68 , G01N1/18 , G01N31/00
CPC分类号： C12N15/1017 , G01N33/54333
摘要： An apparatus, method and kit for isolating a biomolecule from a sample. The sample comprises a complex biological material, which includes insoluble matter. Some embodiments of the apparatus and kit include a reservoir and means for capturing the biomolecule either contained within or coupled to the reservoir. The reservoir can have an inner surface, and can be adapted to contain the sample. The apparatus can further include least one of a filter positioned between the means for capturing the biomolecule and at least a portion of the inner surface of the reservoir, and an aperture defined in the inner surface of the reservoir. Some embodiments of the method include combining the sample. with a solid phase that is adapted to capture the biomolecule, removing the insoluble matter from the sample, and removing the biomolecule from the solid phase.

58. 发明公开

EP1588143A4 IMPROVED LUCIFERASE-BASED ASSAYS 有权
标题翻译：改进ASSAYS荧光素酶为基础
公开(公告)号：EP1588143A4
公开(公告)日：2007-02-14
申请号：EP03800272
申请日：2003-12-23
申请人： PROMEGA CORP
发明人： HAWKINS ERIKA , CALI JAMES J , HO SAMUEL KIN SANG , O'BRIEN MARTHA , SOMBERG RICHARD , BULLEIT ROBERT F , WOOD KEITH V
IPC分类号： C12Q1/66 , C12Q1/68
CPC分类号： C12Q1/66
摘要： A method and kit is provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferase enzyme activity from interference of the compound and minize interference by at least about 10% relative to an assay not having tolerance enhancement agent.

59. 发明公开

EP1654359A4 SYNTHETIC NUCLEIC ACIDS FROM AQUATIC SPECIES 审中-公开
标题翻译：水生物种的合成核酸
公开(公告)号：EP1654359A4
公开(公告)日：2006-11-22
申请号：EP03819255
申请日：2003-11-20
申请人： PROMEGA CORP
发明人： ALMOND BRIAN D , WOOD MONIKA G , WOOD KEITH V
IPC分类号： C12N15/09 , C12N15/10 , C07H21/04 , C07K14/435 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/02 , C12N15/00 , C12N15/12 , C12N15/67 , C12N15/85
CPC分类号： C12N9/0069 , C07K14/43595 , C12N15/67 , C12N15/8212 , C12N15/8216

60. 发明公开

EP0553234A4 EP0553234A4 - 失效
标题翻译： EP0553234A4 - Google专利
公开(公告)号：EP0553234A4
公开(公告)日：1995-08-02
申请号：EP91919421
申请日：1991-09-09
申请人： PROMEGA CORP
发明人： WOOD KEITH V
IPC分类号： G01N21/76 , C12N9/02 , C12Q1/66
CPC分类号： C12N9/0069 , C12Q1/66 , C12Y113/12007 , Y10S435/81 , Y10S435/968 , Y10S435/975 , Y10S436/808
摘要： Methods and compositions are provided for improved kinetics of light production from luciferase activity in beetle luciferase-luciferin reactions. Thus, the invention provides methods, compositions and test kits for assaying samples for the presence of a beetle luciferase or, using a beetle luciferase-luciferin reaction, the presence of ATP. The invention also provides the complex of the coenzyme, Coenzyme A, a beetle luciferase and oxyluciferin in its exited state in the luciferase-luciferin reaction and luciferyl-CoA, the thioester of Coenzyme A and luciferin (D-(-)-2-(6'-hydroxy-2'-benzothiazolyl)-thiazoline-4-carboxylic acid). The figure depicts the luminescence curves in which a combination of CoA, DTT and BSA were present in the assay, CoA alone was present in the assay, and there were no additions to the conventional assay. The combined effect of CoA, DTT and BSA resulted in total light production greater than 15 times that with no additions.
摘要翻译：提供了方法和组合物用于改善甲虫荧光素酶 - 荧光素反应中萤光素酶活性的光产生动力学。因此，本发明提供了用于分析样品中是否存在甲虫荧光素酶的方法，组合物和测试试剂盒，或者使用甲虫荧光素酶 - 荧光素反应来测定ATP的存在。本发明还提供了在荧光素酶 - 荧光素反应中存在的辅酶，辅酶A，甲虫荧光素酶和羟基荧光素复合物以及荧光素酶-CoA，辅酶A的硫酯和荧光素（D - （ - ） - 2- 6'-羟基-2'-苯并噻唑基） - 噻唑啉-4-羧酸）。该图描绘了在测定中存在CoA，DTT和BSA的组合的发光曲线，在测定中存在单独的CoA，并且没有添加常规测定。 CoA，DTT和BSA的综合效应导致总产光量大于未添加量的15倍。

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