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51. 发明申请

US20150351326A1 Plant Infiltration Device 审中-公开
标题翻译：植物渗透装置
公开(公告)号：US20150351326A1
公开(公告)日：2015-12-10
申请号：US14379202
申请日：2013-02-15
申请人： MEDICAGO INC.
发明人： Marc-Andre D'AOUST , Nicole BECHTOLD , Luc LAURIN , Louis-Philippe VEZINA , Normand DUBUC
IPC分类号： A01G7/06 , A01G1/00 , C12N15/82
CPC分类号： A01G7/06 , A01G7/00 , A01G22/00 , C12N15/8205
摘要： It is provided a plant infiltration device for infiltrating plants with an inoculum and method of using same, the device comprising a frame having one or more tray racks adapted for receiving at least one tray of plants to be infiltrated; an automated manipulation mechanism mounted to the frame and operable to displace the plant trays within the device; a plurality of inoculum reservoirs mounted to the frame and containing one or more inoculum fluids; an infiltration tank mounted to the frame and within which the plant trays are received when disposed in the infiltration position; and a vacuum generating device in communication with the infiltration tank to apply a negative pressure within the infiltration tank, thereby opening pores of the plant leaves immersed in the inoculum within the infiltration tank and causing the inoculum fluid to infiltrate into the plant leaves.
摘要翻译：提供了一种用于用接种物渗透植物的植物浸渗装置及其使用方法，所述装置包括具有一个或多个适于接收至少一个待渗透的植物盘的一个或多个托盘架的框架; 安装到所述框架并且可操作以移动所述装置内的所述植物托盘的自动操作机构; 多个接种物贮存器，其安装在框架上并含有一种或多种接种液; 安装在所述框架上并且当设置在所述渗透位置时所述植物托盘被接收的渗透罐; 以及与渗透槽连通以在渗透槽内施加负压的真空产生装置，从而打开浸入渗透罐内的接种物中的植物叶片的孔，并使接种液渗入植物叶中。

52. 发明授权

US11884929B2 CPMV enhancer elements 有权
公开(公告)号：US11884929B2
公开(公告)日：2024-01-30
申请号：US16880583
申请日：2020-05-21
申请人： Medicago Inc.
发明人： Pierre-Olivier Lavoie , Marc-Andre D'Aoust
IPC分类号： C12N15/82 , C12N7/04
CPC分类号： C12N15/8216 , C12N7/045 , C12N15/8251
摘要： An expression enhancer comprising a CPMV 5′UTR nucleotide sequence consisting of X nucleotides (CMPVX), where X=160, 155, 150, or 114 of SEQ ID NO:1, or consisting of a nucleotide sequence comprising from about 80% to 100% sequence similarity with CMPVX, where X=160, 155, 150, or 114 of SEQ ID NO:1 SEQ ID NO:1 is provided. The expression enhancer may further comprise a stuffer sequence fused to the 3′ end of the 5′UTR nucleotide sequence (CMPVX+, where X=160, 155, 150, or 114 of SEQ ID NO:1). The stuffer sequence may comprise one or more plant kozak sequences. Plants comprising the expression enhancer and methods using the expression enhancer are also described

53. 发明申请

US20230044454A1 RECOMBINANT INFLUENZA VIRUS-LIKE PARTICLES (VLPS) PRODUCED IN TRANSGENIC PLANTS 有权
公开(公告)号：US20230044454A1
公开(公告)日：2023-02-09
申请号：US17815503
申请日：2022-07-27
申请人： Medicago Inc.
发明人： Marc-Andre D'AOUST , Manon COUTURE , Frederic ORS , Sonia TREPANIER , Pierre-Olivier LAVOIE , Michele DARGIS , Louis-Philippe VEZINA , Nathalie LANDRY
IPC分类号： C12N15/82 , A61K39/145 , C07K14/005 , C12N7/00 , C12N9/90 , A61K39/12 , G06F9/50
摘要： A method for synthesizing influenza virus-like particles (VLPs) within a plant or a portion of a plant is provided. The method involves expression of influenza HA in plants and the purification by size exclusion chromatography. The invention is also directed towards a VLP comprising influenza HA protein and plant lipids. The invention is also directed to a nucleic acid encoding influenza HA as well as vectors. The VLPs may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.

54. 发明申请

US20230024533A1 NOROVIRUS FUSION PROTEINS AND VLPS COMPRISING NOROVIRUS FUSION PROTEINS 有权
公开(公告)号：US20230024533A1
公开(公告)日：2023-01-26
申请号：US17735706
申请日：2022-05-03
申请人： MEDICAGO INC.
发明人： Pierre-Olivier LAVOIE , Marc-Andre D'AOUST
IPC分类号： C07K14/005 , C12N7/00 , C12N15/82 , C07K16/10 , A61K39/12
摘要： Nucleic acids encoding norovirus VP1 fusion proteins and VLPs comprising the norovirus VP1 fusion proteins are provided. Methods for norovirus VP1 fusion protein and norovirus VLP production in plants are also described. The VP1 fusion protein comprises, a first sequence encoding an S domain derived from a first norovirus strain, and a second sequence encoding a P domain derived from a second norovirus strain.

55. 发明申请

US20220154195A1 ENDOGENOUS PLANT EXPRESSION ENHANCER 有权
公开(公告)号：US20220154195A1
公开(公告)日：2022-05-19
申请号：US17439155
申请日：2019-03-14
申请人： MITSUBISHI TANABE PHARMA CORPORATION , MEDICAGO INC. , NATIONAL UNIVERSITY CORPORATION NARA INSTITUTE OF SCIENCE AND TECHNOLOGY
发明人： Pierre-Olivier Lavoie , Marc-Andre D'Aoust , Ko Kato , Shotaro Yamasaki
IPC分类号： C12N15/82
摘要： An isolated expression enhancer active in a plant, portion of a plant or plant cell, the expression enhancer is provided. The isolated expression enhancer may be selected from the group consisting of nbEPI42 (SEQ ID NO:1); nbSNS46 (SEQ ID NO:2); nbCSY65 (SEQ ID NO:3); nbHEL40 (SEQ ID NO:4); and nbSEP44 (SEQ ID NO:5). Methods for using the isolated expression enhancer are also provided.

56. 发明申请

US20210023205A1 VIRUS LIKE PARTICLE PRODUCTION IN PLANTS 有权
公开(公告)号：US20210023205A1
公开(公告)日：2021-01-28
申请号：US17067049
申请日：2020-10-09
申请人： MEDICAGO INC.
发明人： Marc-Andre D'Aoust , Manon Couture , Pierre-Olivier Lavoie , Louis-Philippe Vezina
IPC分类号： A61K39/295 , C07K14/005 , C12N15/82 , C12N7/00
摘要： A method of producing a virus like particle (VLP) in a plant, and compositions comprising VLPs, are provided. The method involves introducing a nucleic acid comprising a regulatory region active in the plant and operatively linked to a chimeric nucleotide sequence encoding, in series, an ectodomain from a virus trimeric surface protein or fragment thereof, fused to an influenza transmembrane domain and cytoplasmic tail, into the plant, or portion of the plant, the ectodomain is from a non-influenza virus trimeric surface protein and heterologous with respect to the influenza transmembrane domain, and the cytoplasmic tail. The plant or portion of the plant are incubated under conditions that permit the expression of the nucleic acid, thereby producing the VLP. A VLP produced by this method are also provided.

57. 发明申请

US20200330583A1 MODIFIED NOROVIRUS VP1 PROTEINS AND VLPS COMPRISING MODIFIED NOROVIRUS VP1 PROTEINS 审中-公开
公开(公告)号：US20200330583A1
公开(公告)日：2020-10-22
申请号：US16768524
申请日：2018-11-30
申请人： MEDICAGO INC.
发明人： Pierre-Olivier LAVOIE , Marc-Andre D'AOUST
IPC分类号： A61K39/12 , C07K14/005 , C12N7/00 , C12N15/82 , A61P31/14
摘要： Nucleic acids encoding modified norovirus VP1 proteins, and VLPs comprising one or more of the modified norovirus VP1 proteins are provided. Methods for modified norovirus VP1 protein, and norovirus VLP, production in plants, portions of the plant or a plant cell, are also described.

58. 发明申请

US20200255849A1 INFLUENZA VIRUS-LIKE PARTICLE PRODUCTION IN PLANTS 审中-公开
公开(公告)号：US20200255849A1
公开(公告)日：2020-08-13
申请号：US16516802
申请日：2019-07-19
申请人： MEDICAGO INC.
发明人： Manon COUTURE , Marc-Andre D'AOUST , Louis-Philippe VEZINA
IPC分类号： C12N15/82 , C12N7/00 , A61K39/12 , A61K39/145 , C07K14/005
摘要： A method of producing a virus like particle (VLP) in a plant comprising modified hemagglutinin is provided. The method comprises introducing a nucleic acid comprising a regulatory region active in the plant and operatively linked to a nucleotide sequence encoding a modified influenza hemagglutinin (HA) protein into the plant, or portion of the plant, the modified HA protein comprises a modified proteolytic loop. Followed by incubating the plant or portion of the plant under conditions that permit the expression of the nucleic acids, thereby producing the VLP. The modified proteolytic loop may comprise one or more protease cleavage sites exhibiting reduced or abolished cleavage by a protease. The nucleotide sequence encoding the HA may be selected from the group consisting of B HA, C, H1, H2, H3, H4, H5, H6, H7, H8, H9, H10, H11, H12, H13, H14, H15, and H16. Also described is a virus like particle (VLP) produced by the method, and plants expressing the VLP. The virus like particle (VLP) may comprise plant-specific N-glycans, or modified N-glycans.

59. 发明授权

US10190132B2 Recombinant influenza virus-like particles (VLPs) produced in transgenic plants expressing hemagglutinin 有权
公开(公告)号：US10190132B2
公开(公告)日：2019-01-29
申请号：US15256119
申请日：2016-09-02
申请人： Medicago Inc.
发明人： Marc-Andre D'Aoust , Manon Couture , Frederic Ors , Sonia Trepanier , Pierre-Olivier Lavoie , Michele Dargis , Louis-Philippe Vezina , Nathalie Landry
IPC分类号： C12N7/00 , C12N15/82 , A61K39/145 , C07K14/005 , C12N9/90 , A61K39/12 , G06F9/50 , A61K39/00
摘要： A method for synthesizing influenza virus-like particles (VLPs) within a plant or a portion of a plant is provided. The method involves expression of influenza HA in plants and the purification by size exclusion chromatography. The invention is also directed towards a VLP comprising influenza HA protein and plant lipids. The invention is also directed to a nucleic acid encoding influenza HA as well as vectors. The VLPs may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.

60. 发明授权

US09657305B2 Synthesis of sialic acid in plants 有权
公开(公告)号：US09657305B2
公开(公告)日：2017-05-23
申请号：US14932326
申请日：2015-11-04
申请人： Medicago, Inc. , Centre National de la Recherche Scientifique , Universite De Rouen
发明人： Thomas Paccalet , Muriel Bardor , Christophe Rihouey , Véronique Gomord , Loïc Faye , Patrice Lerouge , Stéphanie Aquin , Louis-Philippe Vezina , Marc-André D'Aoust
IPC分类号： C12N15/82 , C12N9/88 , C12P19/26 , C12P21/00
CPC分类号： C12N15/8245 , C12N9/1085 , C12N9/88 , C12N15/8257 , C12P19/26 , C12P21/005 , C12Y205/01057 , C12Y401/03003
摘要： A method of synthesizing sialic acid in plants, and plants capable of synthesizing sialic acid is provided. Furthermore, a method of producing sialylated protein in a plant is also provided. The method to synthesize sialic acid comprises providing a plant comprising a nucleotide sequence encoding N-acetyl neuraminic acid (Neu5Ac) synthase or Neu5Ac lyase, and expressing the nucleotide sequence thereby synthesizing sialic acid. The plant may also co-express a nucleotide sequence encoding one or more than one of an epimerase, a CMP-Neu5Ac synthase, a CMP-Neu5Ac transporter and a sialyltransferase.

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