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    • 54. 发明申请
    • DNA SEQUENCING
    • DNA序列
    • WO2013166303A1
    • 2013-11-07
    • PCT/US2013/039295
    • 2013-05-02
    • IBIS BIOSCIENCES, INC.ESHOO, Mark W.
    • ESHOO, Mark W.
    • C12Q1/68
    • C12Q1/6869C12Q2525/101C12Q2525/185C12Q2563/107
    • Provided herein is technology relating to sequencing nucleic acids and particularly, but not exclusively, to methods, compositions, systems, and kits for sequencing a nucleic acid using a degenerate two-base code. Particular embodiments provide: 1) that the two-base degenerate code relates a first element to a base comprising adenine (A) or guanine (G) and a second element to a base comprising cytosine (C) or thymine (T); 2) that the two-base degenerate code relates a first element to a base comprising A or C and a second element to a base comprising G or T; and 3) that the two-base degenerate code relates a first element to a base comprising G or C and a second element to a base comprising A or T.
    • 本文提供了与测序核酸有关的技术,特别但并非排他地涉及使用简并二碱基编码对核酸进行测序的方法,组合物,系统和试剂盒。 具体实施方案提供:1)所述双碱基简并代码将第一元件与包含腺嘌呤(A)或鸟嘌呤(G)的碱和第二元素连接至包含胞嘧啶(C)或胸腺嘧啶(T)的碱; 2)两碱性简并代码将第一元素与包含A或C的碱和第二元素相关联到包含G或T的碱; 和3)两碱性简并代码将第一元素与包含G或C的碱和第二元素相关联到包含A或T的碱。