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41. 发明授权

US06890908B1 Modified ribozymes 失效
标题翻译：改良的核酶
公开(公告)号：US06890908B1
公开(公告)日：2005-05-10
申请号：US08936657
申请日：1997-09-24
申请人： Fritz Eckstein , Wolfgang Pieken , Fritz Benseler , David B. Olsen , David M. Williams , Olaf Heidenreich
发明人： Fritz Eckstein , Wolfgang Pieken , Fritz Benseler , David B. Olsen , David M. Williams , Olaf Heidenreich
IPC分类号： A61K31/70 , A61K31/00 , A61K31/712 , A61K38/00 , A61K48/00 , A61P31/18 , C07H21/02 , C12N9/00 , C12N15/00 , C12N15/09 , C12N15/113
CPC分类号： C12N15/1132 , A61K38/00 , C12N15/113 , C12N2310/121 , C12N2310/122 , C12N2310/315 , C12N2310/322
摘要： The present invention refers to an RNA molecule with catalytic activity comprising at least one modified nucleoside, wherein the hydroxy group at the 2′-position of the ribose sugar is replaced by a modifier group, selected from halo, sulfhydryl, azido, amino, monosubstituted amino and disubstituted amino groups, a process for the preparation of modified RNA molecules and the use of modified RNA molecules as therapeutic agents and biocatalysts.
摘要翻译：本发明涉及具有至少一种修饰核苷的催化活性的RNA分子，其中核糖2'-位的羟基被选自卤素，巯基，叠氮基，氨基，单取代的修饰基团氨基和二取代的氨基，制备修饰的RNA分子的方法和使用修饰的RNA分子作为治疗剂和生物催化剂。

42. 发明授权

US06369208B1 Capped synthetic RNA, analogs, and aptamers 失效
标题翻译：封盖的合成RNA，类似物和适体
公开(公告)号：US06369208B1
公开(公告)日：2002-04-09
申请号：US08973137
申请日：1998-03-30
申请人： James L. Cole , Lawrence C. Kuo , David B. Olsen , Fritz Benseler
发明人： James L. Cole , Lawrence C. Kuo , David B. Olsen , Fritz Benseler
IPC分类号： C07H2102
CPC分类号： C12N15/115 , A61K38/00 , C07H21/00 , C12N15/1131 , C12N2310/315 , C12N2310/317 , C12N2310/321 , C12N2310/322 , C12N2310/3513 , C12N2310/3521
摘要： A method is provided for making synthetic capped RNAs. These compounds serve as substrates for the virally encoded endonuclease associated with influenza virus. We are able to assay for this unique and specific viral activity of cleavage of a capped RNA in vitro. Therefore, screening of inhibitors of this activity is possible. In addition, short non-extendible (due to their length or because of the modification of the 3′-end of the oligo, i.e. 3′-dA) RNAs are potent inhibitors of the cleavage of capped RNAs by influenza endonuclease. Finally, these compounds may be used to investigate viral and cellular mechanisms of transcription/translation or mRNA maturation.
摘要翻译：提供了制备合成上限RNA的方法。这些化合物用作与流感病毒相关的病毒编码的内切核酸酶的底物。我们能够测定这种在体外切割加盖RNA的独特且特异的病毒活性。因此，可以筛选该活性的抑制剂。此外，短的不可延伸的（由于其长度或由于寡核苷酸的3'末端的修饰，即3'-dA）RNA是流感内切核酸酶切割加帽RNA的有效抑制剂。最后，这些化合物可用于研究转录/翻译或mRNA成熟的病毒和细胞机制。

43. 发明授权

US5698687A Modified ribozymls 失效
标题翻译：改良的核酶
公开(公告)号：US5698687A
公开(公告)日：1997-12-16
申请号：US434501
申请日：1995-05-04
申请人： Fritz Eckstein , Wolfgang Pieken , Fritz Benseler , David B. Olsen , David M. Williams , Olaf Heindenreich
发明人： Fritz Eckstein , Wolfgang Pieken , Fritz Benseler , David B. Olsen , David M. Williams , Olaf Heindenreich
IPC分类号： A61K31/70 , A61K31/00 , A61K31/712 , A61K38/00 , A61K48/00 , A61P31/18 , C07H21/02 , C12N9/00 , C12N15/00 , C12N15/09 , C12N15/113 , C07H21/04 , C07H21/00 , C12P19/34
CPC分类号： C12N15/1132 , C12N15/113 , A61K38/00 , C12N2310/121 , C12N2310/122 , C12N2310/315 , C12N2310/322
摘要： The present invention refers to an RNA molecule with catalytic activity comprising at least one modified nucleoside, wherein the hydroxy group at the 2'-position of the fibose sugar is replaced by a modifier group, selected from halo, sulfhydryl, azido, amino, monosubstituted amino, and disubstituted andno groups, a process for the preparation of modified KNA molecules and the use of modified KNA molecules as therapeutic agents and biocatalysts.
摘要翻译：本发明涉及具有至少一种修饰核苷的催化活性的RNA分子，其中纤维糖2位的羟基被选自卤素，巯基，叠氮基，氨基，单取代的修饰基团氨基和二取代和无基团，制备改性KNA分子的方法和使用修饰的KNA分子作为治疗剂和生物催化剂。

44. 发明授权

US5660989A DNA polymerase extension assay for influenza virus endonuclease 失效
标题翻译：流感病毒内切核酸酶的DNA聚合酶延伸测定
公开(公告)号：US5660989A
公开(公告)日：1997-08-26
申请号：US487759
申请日：1995-06-07
申请人： James L. Cole , Lawrence C. Kuo , David B. Olsen
发明人： James L. Cole , Lawrence C. Kuo , David B. Olsen
IPC分类号： C12Q1/68 , C12Q1/70
CPC分类号： C12Q1/701 , C12Q1/6846
摘要： An assay for the influenza virus endonuclease has been developed which involves DNA polymerase-catalyzed extension of the viral endonuclease cleavage product using labeled nucleotides and a DNA template containing a 3' region complementary to the product joined to a 5' region consisting of repeated residues. The DNA polymerase coupled assay does not involve gel electrophoretic separation and is amenable to high volume screening of potential inhibitors. Another key feature of the assay is that it is sensitive enough to detect 200 attomoles of product.
摘要翻译：已经开发了用于流感病毒核酸内切酶的测定法，其涉及使用标记的核苷酸对病毒核酸内切酶切割产物进行DNA聚合酶催化的延伸，并且包含与连接到由重复残基组成的5'区域的产物互补的3'区域的DNA模板。 DNA聚合酶偶联测定不涉及凝胶电泳分离，并且适于对潜在的抑制剂进行高体积筛选。该测定的另一个关键特征是足够敏感以检测200个产品的阿托莫尔。

45. 发明授权

US5353620A Portable sheet bending brake 失效
标题翻译：便携式薄板弯曲制动
公开(公告)号：US5353620A
公开(公告)日：1994-10-11
申请号：US111978
申请日：1993-08-25
申请人： David B. Olsen , Douglas J. Chubb , Arthur B. Chubb
发明人： David B. Olsen , Douglas J. Chubb , Arthur B. Chubb
IPC分类号： B21D5/04
CPC分类号： B21D5/042
摘要： A sheet bending brake comprises a frame having a fixed jaw and a movable jaw. The fixed jaw defines a clamping surface and the movable jaw has a clamping surface movable between workpiece clamping and non-clamping positions relative to the clamping surface on the fixed jaw. A bending member is hingedly connected to the fixed jaw. A handle is pivoted to the frame. A plurality of axially extensible links are provided. Each link is pivoted at one end to the handle and at the other end to the movable jaw such that in one position of the handle, the links move the movable jaw out of clamping position, and in another position of the handle, the movable jaw is in clamping position. Each link includes an upper block connected to the frame, a shaft extending from the upper block and rotatable therein and threaded into a lower block pivoted to the movable jaw. The shaft has a socket connection in the upper end thereof accessible through an opening in the upper block.
摘要翻译：片材弯曲制动器包括具有固定夹爪和活动夹爪的框架。固定夹爪限定夹紧表面，并且可动夹爪具有夹紧表面，该夹紧表面可相对于固定夹爪上的夹紧表面在工件夹紧位置和非夹紧位置之间移动。弯曲构件铰接地连接到固定夹爪。手柄枢转到框架。提供多个可轴向延伸的连杆。每个连杆在一端枢转到手柄，另一端枢转到可动夹爪，使得在手柄的一个位置处，连杆将可动夹爪移动到夹紧位置之外，并且在手柄的另一位置，可动夹爪处于夹紧位置。每个连杆包括连接到框架的上部块，从上部块延伸并可在其中旋转并旋入到可枢转到可动夹爪的下部块中的轴。轴在其上端具有通过上部块中的开口可接近的插座连接。

46. 发明授权

US4995452A Filter-conditioner for motor cooling liquid 失效
标题翻译：电机冷却液过滤器
公开(公告)号：US4995452A
公开(公告)日：1991-02-26
申请号：US521834
申请日：1983-08-10
申请人： Gerald R. Franck , David B. Olsen
发明人： Gerald R. Franck , David B. Olsen
IPC分类号： F01P11/06 , F28F19/01
CPC分类号： F01P11/06 , F28F19/01 , F01P2011/061
摘要： An engine cooling system including a filter connected in a radiator overflow system so that engine coolant flowing from the radiator to an overflow reservoir under the influence of pressure in the radiator will flow through the filter, whereas fluid flowing from the overflow reservoir to the radiator under the influence of atmospheric pressure when the pressure in the radiator drops will draw filtered engine coolant from the reservoir.
摘要翻译：一种发动机冷却系统，其包括连接在散热器溢流系统中的过滤器，使得在散热器中的压力下，从散热器流向溢流容器的发动机冷却剂将流过过滤器，而流体从溢流容器流到散热器下方当散热器中的压力下降时，大气压力的影响将从储存器中抽出过滤的发动机冷却液。

47. 发明授权

US4951540A Shingle ridge cap cutter 失效
标题翻译：瓦楞脊切刀
公开(公告)号：US4951540A
公开(公告)日：1990-08-28
申请号：US324309
申请日：1989-03-16
申请人： James Cross , Jerry Henning , James J. Rhoades , Arthur B. Chubb , David B. Olsen
发明人： James Cross , Jerry Henning , James J. Rhoades , Arthur B. Chubb , David B. Olsen
IPC分类号： B26D1/30 , B26D7/22 , E04D1/30 , E04D15/02
CPC分类号： B26D1/305 , B26D7/22 , E04D1/30 , E04D15/02 , E04D2001/305 , Y10S83/01 , Y10T83/606 , Y10T83/7593 , Y10T83/872 , Y10T83/8749 , Y10T83/8786 , Y10T83/8801 , Y10T83/8812 , Y10T83/9447 , Y10T83/9449 , Y10T83/949
摘要： A shingle ridge cap cutter utilized for cutting uniform sections from roof shingles comprising a frame along which a shingle is guided to a manually operated cutter that comprises spaced blades that are moved to sever a section of the shingle. The cutter blades are provided in spaced-apart, non parallel relationship so that a combined rectangular and trapezoidal-shaped ridge cap is made by single movement of the cutter with the roof shingle positioned so that the cutter blades are aligned with the slots along one edge of the shingle. Provision is made for operation to prevent injury to the hands of the worker.
摘要翻译：一种用于从屋顶瓦板切割均匀部分的瓦片脊盖切割器，包括框架，木瓦沿着框架被引导到手动操作的切割器，该切割器包括被移动以切断木瓦的一部分的间隔开的叶片。切割刀片以间隔开的非平行关系设置，使得组合的矩形和梯形形状的脊盖通过切割器的单次运动制成，其中屋顶木瓦定位成使得切割刀片沿着一个边缘与槽对齐的木瓦。规定作业，以防止工人手中的伤害。

48. 发明授权

US4444247A Filter-conditioner for motor cooling liquid 失效
标题翻译：电机冷却液过滤器
公开(公告)号：US4444247A
公开(公告)日：1984-04-24
申请号：US303520
申请日：1981-09-18
申请人： Gerald R. Franck , David B. Olsen
发明人： Gerald R. Franck , David B. Olsen
IPC分类号： F01P11/06 , F28F19/01 , F28F19/00
CPC分类号： F28F19/01 , F01P11/06 , F01P2011/061
摘要： An engine cooling system including a filter connected in a radiator overflow system so that fluid flowing from the radiator to an overflow reservoir under the influence of pressure in the radiator will flow around the filter, whereas fluid flowing from the overflow reservoir to the radiator under the influence of atmospheric pressure when the pressure in the radiator drops will pass slowly through the filter.
摘要翻译：一种发动机冷却系统，包括连接在散热器溢流系统中的过滤器，使得在散热器内的压力影响下从散热器流向溢流容器的流体将流过过滤器，而流体从溢流容器流到散热器下方的散热器当散热器中的压力下降时，大气压力的影响将缓慢地通过过滤器。

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