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41. 发明授权

US08317990B2 Droplet actuator loading and target concentration 有权
标题翻译：液滴致动器负载和目标浓度
公开(公告)号：US08317990B2
公开(公告)日：2012-11-27
申请号：US12531835
申请日：2008-03-24
申请人： Vamsee K. Pamula , Michael G. Pollack , Vijay Srinivasan
发明人： Vamsee K. Pamula , Michael G. Pollack , Vijay Srinivasan
IPC分类号： G01N35/08 , G01N33/543
CPC分类号： C25B15/00 , B01F13/0071 , B01L3/502761 , B01L3/502792 , B01L2200/0605 , B01L2200/0647 , B01L2200/0668 , B01L2200/0673 , B01L2300/0636 , B01L2300/0816 , B01L2300/0861 , B01L2300/0867 , B01L2300/089 , B01L2400/0427 , B01L2400/0487 , G01N33/5438
摘要： A droplet actuator and method of providing a droplet comprising an target substance on the droplet actuator, and including discrete flow and continuous flow functionality. Discrete flow function is controlled by electrodes arranged for conducting droplet operations on a substrate surface. The continuous flow function includes a fluid path arranged for flowing a fluid therethrough. The discrete flow and continuous flow functions may be by a barrier, including a second fluid path through the barrier. The continuous flow function may include a capture surface comprising a component having an affinity for the target substance. Methods of the invention may include flowing a fluid comprising the target substance through the fluid path; capturing the target substance on the capture surface; and forming a droplet in the discrete flow region via the second fluid path and the electrodes comprising the captured target substance.
摘要翻译：液滴致动器和在液滴致动器上提供包含目标物质的液滴的方法，并且包括离散流动和连续流动功能。离散流动功能由布置用于在基板表面上进行液滴操作的电极控制。连续流动功能包括设置成使流体流过其中的流体路径。离散的流动和连续流动功能可以是通过屏障，包括通过屏障的第二流体路径。连续流动功能可以包括包含对目标物质具有亲和性的成分的捕获表面。本发明的方法可以包括使包含目标物质的流体流过流体路径; 捕获表面上的目标物质; 以及经由所述第二流体路径在所述离散流动区域中形成液滴，并且所述电极包括所捕获的目标物质。

42. 发明授权

US08313895B2 Droplet-based surface modification and washing 有权
标题翻译：基于液滴的表面改性和洗涤
公开(公告)号：US08313895B2
公开(公告)日：2012-11-20
申请号：US12615609
申请日：2009-11-10
申请人： Michael G. Pollack , Vamsee K. Pamula , Vijay Srinivasan , Allen E. Eckhardt , Richard B. Fair
发明人： Michael G. Pollack , Vamsee K. Pamula , Vijay Srinivasan , Allen E. Eckhardt , Richard B. Fair
IPC分类号： C12Q1/00
CPC分类号： G01N27/26 , B01F13/0071 , B01F13/0076 , B01L3/0268 , B01L3/502715 , B01L3/502723 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0668 , B01L2200/0673 , B01L2200/0684 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , B01L2400/0448 , C12Q1/686 , C12Q1/6869 , G01N27/3271 , G01N27/416 , G01N27/447 , G01N35/00029 , G01N2035/00158 , G01N2035/00237
摘要： The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of providing a bead-containing droplet with a reduced concentration of a substance is provided, the method including providing a droplet microactuator including a droplet having a starting volume and including one or more beads and a starting concentration and starting quantity of the substance. The method further includes conducting one or more droplet operations to merge a wash droplet with the droplet provided in the above step to yield a combined droplet and including one or more droplet operations to divide the combined droplet to yield a set of droplets including a droplet including substantially all of the one or more beads and having a decreased concentration of the substance relative to the starting concentration and a droplet which is substantially lacking in the beads.
摘要翻译：本发明涉及液滴表面改性和洗涤。根据一个实施例，提供了一种提供具有降低的物质浓度的含珠粒的液滴的方法，所述方法包括提供包括具有起始体积并包括一个或多个珠粒和起始浓度的液滴的液滴微致动器，并起始浓度物质的数量。该方法还包括进行一个或多个液滴操作以将洗涤液滴与上述步骤中提供的液滴合并以产生组合液滴并且包括一个或多个液滴操作以分开组合的液滴以产生包括液滴的一组液滴，包括液滴，包括基本上所有的一种或多种珠粒，并且物质相对于起始浓度降低，而珠子中基本上不含有液滴。

43. 发明申请

US20120165238A1 Droplet-Based Surface Modification and Washing 有权
标题翻译：基于液滴的表面改性和洗涤
公开(公告)号：US20120165238A1
公开(公告)日：2012-06-28
申请号：US12113385
申请日：2008-05-01
申请人： Vamsee K. Pamula , Vijay Srinivasan , Allen E. Eckhardt , Michael G. Pollack , Richard B. Fair
发明人： Vamsee K. Pamula , Vijay Srinivasan , Allen E. Eckhardt , Michael G. Pollack , Richard B. Fair
IPC分类号： G01N33/53 , C12M1/34 , C25B9/00 , C23C16/44 , C25B15/00 , C12M1/40 , C11D7/60
CPC分类号： G01N27/26 , B01F13/0071 , B01F13/0076 , B01L3/0268 , B01L3/502715 , B01L3/502723 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0668 , B01L2200/0673 , B01L2200/0684 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , B01L2400/0448 , C12Q1/686 , C12Q1/6869 , G01N27/3271 , G01N27/416 , G01N27/447 , G01N35/00029 , G01N2035/00158 , G01N2035/00237
摘要： The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of modifying a surface on a droplet microactuator is provided, wherein the method includes executing one or more droplet operations to bring a droplet comprising a surface-modifying agent into contact with the surface. According to another embodiment, a droplet microactuator is provided and includes a sample or reagent immobilized on a surface thereof and arranged such that a droplet on the droplet microactuator may contact the surface. According to yet another embodiment, a method of removing a substance from a surface to which the substance is bound is provided, the method including conducting one or more droplet operations to contact a droplet with the surface, the droplet comprising a solution for eluting the substance from the surface
摘要翻译：本发明涉及液滴表面改性和洗涤。根据一个实施例，提供了修改液滴微致动器上的表面的方法，其中所述方法包括执行一个或多个液滴操作以使包含表面改性剂的液滴与表面接触。根据另一个实施例，提供液滴微致动器，并且包括固定在其表面上的样品或试剂，并且布置成使得液滴微致动器上的液滴可接触表面。根据另一个实施方案，提供从物质结合的表面除去物质的方法，所述方法包括进行一个或多个液滴操作以使液滴与表面接触，所述液滴包含用于洗脱物质的溶液从表面

44. 发明授权

US08007739B2 Protein crystallization screening and optimization droplet actuators, systems and methods 有权
标题翻译：蛋白质结晶筛选和优化液滴执行器，系统和方法
公开(公告)号：US08007739B2
公开(公告)日：2011-08-30
申请号：US11839744
申请日：2007-08-16
申请人： Michael G. Pollack , Vamsee K. Pamula , Allen E. Eckhardt , Vijay Srinivasan
发明人： Michael G. Pollack , Vamsee K. Pamula , Allen E. Eckhardt , Vijay Srinivasan
IPC分类号： B01L3/00 , G01N1/00
CPC分类号： B01L3/502784 , B01J19/0046 , B01J2219/00466 , B01J2219/00495 , B01J2219/00587 , B01J2219/00725 , B01L3/502761 , B01L2200/0642 , B01L2200/0647 , B01L2300/0819 , B01L2300/0829 , B01L2300/0864 , B01L2300/0887 , B01L2300/0893 , B01L2300/18 , B01L2400/0427 , B01L2400/043 , C07K1/1136 , C30B7/00 , C30B29/58 , Y10T436/2575
摘要： Protein crystallization screening and optimization droplet actuators, systems and methods are provided. According to one embodiment, a screening droplet actuator is provided and includes: (a) a port for introduction of one or more crystallization reagents and/or one or more protein solutions; and (b) a substrate including: (i) an array of two or more mixing wells; and (ii) electric field mediated microfluidics for moving droplets comprising the crystallization reagents and protein solutions into the mixing wells. Optimization droplet actuators, systems including screening droplet actuators, methods of screening protein crystallization conditions, and methods of testing conditions for growing a crystal are also provided.
摘要翻译：提供蛋白质结晶筛选和优化液滴致动器，系统和方法。根据一个实施例，提供了一种筛选液滴致动器，其包括：（a）用于引入一种或多种结晶试剂和/或一种或多种蛋白质溶液的端口; 和（b）基材，包括：（i）两个或更多个混合孔的阵列; 和（ii）用于将包含结晶试剂和蛋白质溶液的液滴移动到混合孔中的电场介导的微流体。还提供了优化液滴致动器，包括筛选液滴致动器的系统，筛选蛋白质结晶条件的方法以及测试晶体生长条件的方法。

45. 发明申请

US20110186433A1 Droplet-Based Particle Sorting 有权
标题翻译：基于液滴的粒子分选
公开(公告)号：US20110186433A1
公开(公告)日：2011-08-04
申请号：US13030418
申请日：2011-02-18
申请人： Michael G. Pollack , Vamsee K. Pamula , Vijay Srinivasan
发明人： Michael G. Pollack , Vamsee K. Pamula , Vijay Srinivasan
IPC分类号： G01N27/447 , C25B9/00 , C25B15/00
CPC分类号： G01N27/447 , B01L3/502761 , B01L3/502792 , B01L2200/0668 , B01L2200/0673 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2400/0427 , C12Q1/6806 , G01N15/1031 , G01N27/44717 , G01N27/44721 , G01N33/543 , G01N35/10 , G01N2015/0065 , G01N2015/0088 , G01N2015/1081 , G01N2035/1034 , Y10T436/11 , Y10T436/118339 , Y10T436/25 , Y10T436/2575
摘要： The present invention relates to droplet-based particle sorting. According to one embodiment, a droplet microactuator is provided and includes: (a) a suspension of particles; and (b) electrodes arranged for conducting droplet operations using droplets comprising particles. A method of transporting a particle is also provided, wherein the method includes providing a droplet comprising the particle and transporting the droplet on a droplet microactuator.
摘要翻译：本发明涉及基于液滴的颗粒分选。根据一个实施例，提供了液滴微致动器，其包括：（a）颗粒悬浮液; 和（b）布置成使用包含颗粒的液滴进行液滴操作的电极。还提供了输送颗粒的方法，其中所述方法包括提供包含颗粒的液滴并将液滴输送到液滴微致动器上。

46. 发明授权

US07939021B2 Droplet actuator analyzer with cartridge 有权
标题翻译：液滴执行器分析仪带墨盒
公开(公告)号：US07939021B2
公开(公告)日：2011-05-10
申请号：US11838450
申请日：2007-08-14
申请人： Gregory F. Smith , Ryan A. Sturmer , Philip Y. Paik , Vijay Srinivasan , Michael G. Pollack , Vamsee K. Pamula , Keith R. Brafford , Richard M. West
发明人： Gregory F. Smith , Ryan A. Sturmer , Philip Y. Paik , Vijay Srinivasan , Michael G. Pollack , Vamsee K. Pamula , Keith R. Brafford , Richard M. West
IPC分类号： G01N33/00 , G01N15/06 , G01N27/00 , G01N21/00 , B65D35/00 , B65D35/28 , G05B21/00
CPC分类号： B01F13/0071 , B01F13/0076 , B01L3/502715 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0668 , B01L2200/148 , B01L2200/16 , B01L2300/024 , B01L2300/0654 , B01L2300/0816 , B01L2300/0819 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , B01L2400/0448 , B01L2400/0683 , G01N33/49
摘要： A droplet actuator with cartridge is provided. According to one embodiment, a sample analyzer is provided and includes an analyzer unit comprising electronic or optical receiving means, a cartridge comprising self-contained droplet handling capabilities, and a wherein the cartridge is coupled to the analyzer unit by a means which aligns electronic and/or optical outputs from the cartridge with electronic or optical receiving means on the analyzer unit. According to another embodiment, a sample analyzer is provided and includes a sample analyzer comprising a cartridge coupled thereto and a means of electrical interface and/or optical interface between the cartridge and the analyzer, whereby electrical signals and/or optical signals may be transmitted from the cartridge to the analyzer.
摘要翻译：提供带墨盒的液滴致动器。根据一个实施例，提供了样本分析器，并且包括分析器单元，其包括电子或光学接收装置，包括独立液滴处理能力的盒，以及其中所述盒通过使电子和 /或来自带有分析仪单元上的电子或光学接收装置的盒的光学输出。根据另一个实施例，提供了一个样本分析器，它包括一个样本分析器，该样本分析器包括与其相连接的盒，以及盒和分析器之间的电接口和/或光学接口的装置，由此电信号和/或光信号可以从墨盒进入分析仪。

47. 发明申请

US20100194408A1 Capacitance Detection in a Droplet Actuator 有权
标题翻译：滴液致动器中的电容检测
公开(公告)号：US20100194408A1
公开(公告)日：2010-08-05
申请号：US12527208
申请日：2008-02-15
申请人： Ryan A. Sturmer , Michael G. Pollack , Vamsee K. Pamula , Vijay Srinivasan , Philip Y. Paik
发明人： Ryan A. Sturmer , Michael G. Pollack , Vamsee K. Pamula , Vijay Srinivasan , Philip Y. Paik
IPC分类号： G01R27/26
CPC分类号： B01L3/50273 , B01L3/502715 , B01L3/502784 , B01L2200/0605 , B01L2300/0627 , B01L2300/0636 , B01L2300/0645 , B01L2300/0809 , B01L2400/0427 , G01N27/22 , G01N27/223 , G01N27/227 , G01N2203/00 , G01V3/02
摘要： A method, circuit and apparatus for detecting capacitance on a droplet actuator, inter alia, for determining the presence, partial presence or absence of a droplet at an electrode on a droplet actuator by: (a) providing a droplet actuator comprising: (i) a substrate comprising electrodes arranged on the substrate for conducting droplet operations on a surface of the substrate; (ii) a capacitance detection circuit for detecting capacitance at the droplet operations surface at one or more of the electrodes; (b) detecting capacitance at the droplet operations surface at one or more of the electrodes; and (c) determining from the capacitance the presence, partial presence or absence of a droplet at the droplet operations surface at the electrode.
摘要翻译：一种用于检测液滴致动器上电容的方法，电路和装置，特别是用于通过以下步骤确定液滴致动器上的电极处的液滴的存在，部分存在或不存在：（a）提供液滴致动器，其包括：（i）基板，其包括布置在所述基板上的电极，用于在所述基板的表面上进行液滴操作; （ii）电容检测电路，用于检测一个或多个电极处的液滴操作表面处的电容; （b）在一个或多个电极处检测液滴操作表面处的电容; 和（c）根据电容确定在电极处的液滴操作表面处的液滴的存在，部分存在或不存在。

48. 发明申请

US20100116640A1 Droplet-Based Surface Modification and Washing 有权
标题翻译：基于液滴的表面改性和洗涤
公开(公告)号：US20100116640A1
公开(公告)日：2010-05-13
申请号：US12615666
申请日：2009-11-10
申请人： Vamsee K. Pamula , Vijay Srinivasan , Allen E. Eckhardt , Michael G. Pollack , Richard B. Fair
发明人： Vamsee K. Pamula , Vijay Srinivasan , Allen E. Eckhardt , Michael G. Pollack , Richard B. Fair
IPC分类号： B01D57/02
CPC分类号： G01N27/26 , B01F13/0071 , B01F13/0076 , B01L3/0268 , B01L3/502715 , B01L3/502723 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0668 , B01L2200/0673 , B01L2200/0684 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , B01L2400/0448 , C12Q1/686 , C12Q1/6869 , G01N27/3271 , G01N27/416 , G01N27/447 , G01N35/00029 , G01N2035/00158 , G01N2035/00237
摘要： The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of splitting a droplet is provided, the method including providing a droplet microactuator including a droplet including one or more beads and immobilizing at least one of the one or more beads. The method further includes conducting one or more droplet operations to divide the droplet to yield a set of droplets including a droplet including the one or more immobilized beads and a droplet substantially lacking the one or more immobilized beads.
摘要翻译：本发明涉及液滴表面改性和洗涤。根据一个实施例，提供了一种分离液滴的方法，所述方法包括提供包括包含一个或多个珠并且固定所述一个或多个珠中的至少一个珠的液滴的液滴微致动器。该方法还包括进行一个或多个液滴操作以分开液滴以产生一组液滴，包括包含一个或多个固定化珠粒的液滴和基本上不含一个或多个固定化珠粒的液滴。

49. 发明申请

US20100025250A1 Droplet Actuator Structures 审中-公开
标题翻译：液滴致动器结构
公开(公告)号：US20100025250A1
公开(公告)日：2010-02-04
申请号：US12529041
申请日：2008-03-03
申请人： Vamsee K. Pamula , Michael G. Pollack , Vijay Srinivasan , Philip Paik
发明人： Vamsee K. Pamula , Michael G. Pollack , Vijay Srinivasan , Philip Paik
IPC分类号： B41J2/045 , B03C9/00
CPC分类号： B01L3/502792 , B01L3/0241 , B01L3/50273 , B01L2200/06 , B01L2300/06 , B01L2400/0427 , B41J2/14
摘要： The objective of this research is to model and design a microfluidic system that uses electrostatic fields to induce movement of discrete droplets of solution. Of particular interest is movement of droplets of H2O for use in biological testing with lab-on a-chip and μTAS systems. Using computer modeling, the electric-fields for planar electrode configurations positioned on an insulating substrate are calculated for a hemispherical drop of H2O on the substrate at various positions. From these electric-fields the force on the drop is calculated. These models show that electrostatic actuation of droplets of H2O is possible. However, as the complexity of the model increases the properties of the system become less desirable and actuation may not be possible. Using microfabrication techniques, the modeled microfluidic systems have been built for testing using a Kapton substrate with copper electrodes. Hexadecenyltrichlorosilane (HTS), a self-assembled monolayer, and its oxidant have been studied and found capable of providing hydrophobic and hydrophilic surface coatings for the systems.
摘要翻译：本研究的目的是建立和设计一个使用静电场诱导离散液滴移动的微流体系统。特别令人感兴趣的是使用实验室上的芯片和muTAS系统用于生物测试的H 2 O滴。使用计算机建模，计算位于绝缘基板上的平面电极配置的电场，用于在不同位置的衬底上的半球形H 2 O的下降。从这些电场计算出液滴上的力。这些模型显示H2O的液滴的静电驱动是可能的。然而，随着模型的复杂性增加，系统的性能变得不太可取，并且致动可能是不可能的。使用微细加工技术，已经建立了用于使用具有铜电极的Kapton衬底进行测试的建模的微流体系统。已经研究了十六碳烯三氯硅烷（HTS），一种自组装单层及其氧化剂，能够为系统提供疏水和亲水的表面涂层。

50. 发明申请

US20090280476A1 Droplet-based affinity assay device and system 有权
标题翻译：基于液滴的亲和力测定装置和系统
公开(公告)号：US20090280476A1
公开(公告)日：2009-11-12
申请号：US11639822
申请日：2006-12-15
申请人： Vijay Srinivasan , Vamsee K. Pamula , Allen E. Eckhardt , Michael G. Pollack
发明人： Vijay Srinivasan , Vamsee K. Pamula , Allen E. Eckhardt , Michael G. Pollack
IPC分类号： G01N33/543 , C12M1/34
CPC分类号： C12Q1/68 , B01L7/52 , B01L2300/0645 , B01L2300/1822 , B01L2300/1894 , B01L2400/0424 , B01L2400/0427 , C12Q1/6869 , G01N27/44791 , Y10T436/25 , C12Q2565/629 , C12Q2565/301 , C12Q2563/149
摘要： The present invention relates to a droplet-based affinity assay device and system. According to one embodiment, a droplet microactuator is provided and includes an antibody immobilized on a surface. According to another embodiment, a droplet microactuator is provided and includes a droplet on the droplet microactuator, the droplet comprising an antibody.
摘要翻译：本发明涉及一种基于液滴的亲和测定装置和系统。根据一个实施例，提供液滴微致动器并且包括固定在表面上的抗体。根据另一个实施例，提供液滴微致动器并且在液滴微致动器上包括液滴，所述液滴包含抗体。

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