专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

41. 发明授权

US4547569A Intercalating agents specifying nucleotides 失效
标题翻译：指定核苷酸的插入剂
公开(公告)号：US4547569A
公开(公告)日：1985-10-15
申请号：US620801
申请日：1984-06-14
申请人： Robert L. Letsinger , Margaret E. Schott
发明人： Robert L. Letsinger , Margaret E. Schott
IPC分类号： C07H21/00 , C12Q1/68 , C07H19/10
CPC分类号： C07H21/00 , C12Q1/68
摘要： This invention discloses a molecule bearing a biologically active group (a phenanthridinium moiety) joined covalently to an oligonucleotide recognition system (a phosphorus nucleoside group) by a linker that permits intercalation of the active group in the pocket formed by the nucleotide and complementary bases in another polynucleotide strand. This class of compounds is illustrated by the following: ##STR1## where R is selected from the group consisting of --NH.sub.2, methylamino, or ethylamino or hydrogen;where R' is selected from the group consisting of --NH.sub.2, methylamino, or ethylamino or hydrogen;where the chain linking the linker group to the phenanthridinium group (R") consists of 2-8 --CH.sub.2 -- groups.
摘要翻译：本发明公开了一种具有生物活性基团（phenanthridinium部分）的分子，其通过接头共价连接到寡核苷酸识别系统（磷核苷组），该接头允许活性基团插入由另一个核苷酸和互补碱基形成的口袋中多核苷酸链。该类化合物由以下说明：其中R选自-NH 2，甲氨基或乙氨基或氢; 其中R'选自-NH 2，甲基氨基或乙基氨基或氢; 其中连接基团与菲啶基（R“）的链由2-8个-CH 2 - 基团组成。

42. 发明授权

US6017700A Cationic oligonucleotides, and related methods of synthesis and use 失效
标题翻译：阳离子寡核苷酸，以及相关的合成和使用方法
公开(公告)号：US6017700A
公开(公告)日：2000-01-25
申请号：US693831
申请日：1996-07-31
申请人： Thomas Horn , Robert L. Letsinger , Tanjore N. Balasubramaniam
发明人： Thomas Horn , Robert L. Letsinger , Tanjore N. Balasubramaniam
IPC分类号： C12N15/09 , C07H21/00 , C12Q1/68 , C07H19/00 , C07H21/02 , C07H21/04
CPC分类号： C07H21/00 , C12Q1/68 , C12Q1/6839
摘要： Novel oligonucleotides are provided having cationic internucleoside linkages. The cationic internucleoside linkage has the structure ##STR1## wherein W, X, Y, Z, R.sup.1, R.sup.2, and R.sup.3 are as defined herein, P* represents an asymmetric phosphorus atom capable of existing in two distinct stereoisomeric configurations, and further wherein the internucleoside linkage is stereouniform. Certain of these oligonucleotides may have alternating anionic and cationic internucleoside linkages, which are not necessarily stereouniform. A method for synthesizing the compounds are provided as well, as are methods for using the compounds, e.g., as antisense molecules and in nucleic acid hybridization assays.
摘要翻译：提供具有阳离子核苷间键的新型寡核苷酸。阳离子核苷间键具有其中W，X，Y，Z，R 1，R 2和R 3如本文所定义的结构，P *表示能够以两种不同立体异构构型存在的不对称磷原子，并且其中核苷间键立体声。这些寡核苷酸中的某些可以具有交替的阴离子和阳离子核苷间键，其不一定是立体构型。还提供了合成化合物的方法，以及使用化合物的方法，例如作为反义分子和在核酸杂交测定中。

43. 发明授权

US5476930A Non-enzymatic ligation of oligonucleotides 失效
标题翻译：寡核苷酸的非酶连接
公开(公告)号：US5476930A
公开(公告)日：1995-12-19
申请号：US376688
申请日：1995-01-23
申请人： Robert L. Letsinger , Sergei M. Gryaznov
发明人： Robert L. Letsinger , Sergei M. Gryaznov
IPC分类号： C12N15/09 , A61K31/7088 , A61K48/00 , A61P31/12 , A61P35/00 , A61P43/00 , C07H21/00 , C07H21/04 , C12Q1/68 , C07H1/00
CPC分类号： C07H21/00 , C12Q1/6813
摘要： A process for non-enzymatic convalent ligation of two oligonucleotides having the structure shown below: ##STR1## The spontaneous reaction of these above oligonucleotides produces a thiophosphorylacetylamino linkage as shown below: ##STR2## When carried out in the presence of a template, this reaction gives 90% yields within 20 minutes at micromolar concentrations.
摘要翻译：具有以下结构的两种寡核苷酸的非酶共价连接的方法：这些上述寡核苷酸的自发反应产生如下所示的硫代磷酰基乙酰氨基键：当在模板存在下进行时，该反应在微摩尔浓度下在20分钟内产生90％产率。

44. 发明授权

US5416203A Steroid modified oligonucleotides 失效
标题翻译：类固醇修饰寡核苷酸
公开(公告)号：US5416203A
公开(公告)日：1995-05-16
申请号：US97320
申请日：1993-07-26
申请人： Robert L. Letsinger
发明人： Robert L. Letsinger
IPC分类号： A61K31/704 , A61K31/7088 , A61P31/12 , A61P31/18 , C07H21/00 , C07J43/00 , C07J51/00
CPC分类号： C07H21/00 , A61K47/48038 , A61K47/48123 , C07J51/00
摘要： An oligonucleotide conjugated to a steroid the oligonucleotide comprising: ##STR1## wherein A is selected from the group consisting of an aliphatic alkyl, branched aliphatic alkyl and an alkyl (branched) chain of 2 to 18 carbon atoms, R is selected from the group consisting of H and lower alkyl up to 12 carbon atoms; B is a naturally occurring base, the steroid is bound to the oligonucleotide utilizing the naturally occurring bases through an (-oxycarbonyl-amino)-decylamine-moiety, and NUC is an oligonucleotide.
摘要翻译：与寡核苷酸缀合的寡核苷酸包含：其中A选自脂族烷基，支链脂族烷基和2至18个碳原子的烷基（支链），R选自：的H和低至12个碳原子的低级烷基; B是天然存在的碱基，使用天然存在的碱通过（ - 羰基 - 氨基） - 十九胺 - 部分结合寡核苷酸，并且NUC是寡核苷酸。

45. 发明授权

US5112962A Labile anchors for solid phase polynucleotide synthesis 失效
标题翻译：固相多核苷酸合成的不规则锚
公开(公告)号：US5112962A
公开(公告)日：1992-05-12
申请号：US612216
申请日：1990-11-09
申请人： Robert L. Letsinger , Charles N. Singman
发明人： Robert L. Letsinger , Charles N. Singman
IPC分类号： C07H19/04 , C07H21/00
CPC分类号： C07H21/00 , C07H19/04 , Y10T436/143333
摘要： This invention discloses a linker arm for solid support synthesis of oligonucleotides and oligonucleotide derivatives that allows the oligomers to be released relatively quickly under mild conditions. The linker arm comprises the following: ##STR1## The linker arm releases the oligomer in about one minute to about thirty minutes in a manner that leaves the oligonucleotide fully protected.
摘要翻译：本发明公开了一种用于固相支持合成寡核苷酸和寡核苷酸衍生物的接头臂，其允许寡聚体在温和条件下相对快速地释放。连接臂包括以下：连接臂以约1分钟至约30分钟释放低聚物，使寡核苷酸完全受到保护。

46. 发明授权

US4958013A Cholesteryl modified oligonucleotides 失效
标题翻译：胆固醇修饰寡核苷酸
公开(公告)号：US4958013A
公开(公告)日：1990-09-18
申请号：US362200
申请日：1989-06-06
申请人： Robert L. Letsinger
发明人： Robert L. Letsinger
IPC分类号： A61K31/704 , A61K31/7088 , A61P31/12 , A61P31/18 , C07H21/00 , C07J43/00 , C07J51/00
CPC分类号： C07H21/00 , A61K47/48038 , A61K47/48123 , C07J51/00
摘要： Oligonucleotides modified at their backbones by the attachment of cholesteryl are described. The modified oligonucleotides anchor in the cell membrane to serve as a probe and to provide therapeutic activity.
摘要翻译：描述了通过胆固醇的附着在其骨架上修饰的寡核苷酸。修饰的寡核苷酸锚定在细胞膜中用作探针并提供治疗活性。

47. 发明授权

US06602669B2 Method of detection by enhancement of silver staining 有权
公开(公告)号：US06602669B2
公开(公告)日：2003-08-05
申请号：US09903461
申请日：2001-07-11
申请人： Robert L. Letsinger , Viswanadham Garimella
发明人： Robert L. Letsinger , Viswanadham Garimella
IPC分类号： C12Q168
CPC分类号： B82Y15/00 , C12Q2563/137 , G01N27/44721 , G01N27/44726 , Y10S977/924
摘要： The present invention relates to a method for amplifying a detection signal by enhancing or promoting the deposition of additional silver in assay detection systems where the formation of a silver spot serves as a reporter for the presence of a target molecule, including biological polymers (e.g., proteins and nucleic acids) and small molecules.

48. 发明授权

US5780613A Covalent lock for self-assembled oligonucleotide constructs 失效
标题翻译：用于自组装寡核苷酸构建体的共价锁定
公开(公告)号：US5780613A
公开(公告)日：1998-07-14
申请号：US509858
申请日：1995-08-01
申请人： Robert L. Letsinger , Mathias K. Herrlein
发明人： Robert L. Letsinger , Mathias K. Herrlein
IPC分类号： C07H21/00 , C07H21/04 , C12Q1/68
CPC分类号： C07H21/00
摘要： A method of autoligating self-assembled oligonucleotide blocks is disclosed. The method includes the step of displacing a 5' displaceable group by a 3' thiophosphoryl group to form an --OP(O)(O.sup.-)S-- internucleoside linkage.
摘要翻译：公开了一种自组装自组装寡核苷酸嵌段的方法。该方法包括通过3'硫代磷酰基置换5'可置换基团以形成-OP（O）（O-）S-核苷间键的步骤。

49. 发明授权

US5646260A Oligonucleotides having modified internucleoside linkages 失效
标题翻译：具有修饰的核苷间键的寡核苷酸
公开(公告)号：US5646260A
公开(公告)日：1997-07-08
申请号：US471248
申请日：1995-06-06
申请人： Robert L. Letsinger , Sergei M. Gryaznov
发明人： Robert L. Letsinger , Sergei M. Gryaznov
IPC分类号： C07H21/00
CPC分类号： C07H21/00
摘要： Novel oligonucleotides, method for improving the hybridization properties of oligonucleotides and novel processes for preparing 3'-phosphorylated oligonucleotides are disclosed.
摘要翻译：公开了新的寡核苷酸，改进寡核苷酸的杂交性质的方法和制备3'-磷酸化寡核苷酸的新方法。

50. 发明授权

US5932718A Oligonucleotides having modified internucleoside linkages or terminal amino group 失效
标题翻译：具有修饰的核苷间键或末端氨基的寡核苷酸
公开(公告)号：US5932718A
公开(公告)日：1999-08-03
申请号：US659924
申请日：1996-06-07
申请人： Robert L. Letsinger , Sergei M. Gryaznov
发明人： Robert L. Letsinger , Sergei M. Gryaznov
IPC分类号： C07H21/00
CPC分类号： C07H21/00
摘要： Novel oligonucleotides, method for improving the hybridization properties of oligonucleotides and novel processes for preparing 3'-phosphorylated oligonucleotides.
摘要翻译：新型寡核苷酸，改善寡核苷酸杂交性质的方法和制备3'-磷酸化寡核苷酸的新方法。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式