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41. 发明授权

US09340795B2 Genetically modified plant capable of biosynthesizing capsinoid 有权
标题翻译：能够生物合成辣椒素的转基因植物
公开(公告)号：US09340795B2
公开(公告)日：2016-05-17
申请号：US12976526
申请日：2010-12-22
申请人： Hiroaki Kisaka , Yaqin Lang , Ryuji Sugiyama , Tetsuya Miwa , Susumu Yazawa
发明人： Hiroaki Kisaka , Yaqin Lang , Ryuji Sugiyama , Tetsuya Miwa , Susumu Yazawa
IPC分类号： C12N15/82 , C12N15/87 , C12P7/62 , C12P13/02
CPC分类号： C12N15/8243 , C12N15/8218 , C12N15/8285 , C12P7/62 , C12P13/02
摘要： The present invention provides a genetically modified plant that biosynthesizes an increased amount of capsinoids, a method of producing the genetically modified plant, and a production method of capsinoids from the genetically modified plant. More particularly, the present invention provides a genetically modified plant capable of producing capsinoids, which shows a decreased expression or activity of an enzyme that catalyzes an amino group conversion reaction from vanillin to vanillylamine as compared to wild strains, and the like. As a method of suppressing expression or activity of an enzyme, an introduction of DNA encoding an antisense RNA, iRNA, ribozyme or dominant-negative mutant for the target gene, a destruction of the gene by a knockout method, mutagen treatment or transposon insertion, an introduction of a gene encoding an antibody against the enzyme and the like can be mentioned.
摘要翻译：本发明提供了生物合成增加量的类毒素的遗传修饰植物，生物转基因植物的方法以及来自转基因植物的类毒素的制备方法。更具体地，本发明提供能够产生辣椒素的遗传修饰植物，与野生株相比，其表现出从香草醛向香草胺催化氨基转化反应的酶的表达或活性降低。作为抑制酶的表达或活性的方法，引入编码靶基因的反义RNA，iRNA，核酶或显性失活突变体的DNA，通过敲除法，诱变处理或转座子插入破坏基因，可以提及编码针对酶等的抗体的基因的引入。

42. 发明授权

US08110385B2 Linear and membrane-like biodevices and bioreactors 失效
标题翻译：线性和膜状生物装置和生物反应器
公开(公告)号：US08110385B2
公开(公告)日：2012-02-07
申请号：US11169910
申请日：2005-06-30
申请人： Satoshi Konishi , Tetsuya Miwa
发明人： Satoshi Konishi , Tetsuya Miwa
IPC分类号： C12N11/08 , C12N11/04 , C12N5/00
CPC分类号： C12M25/10
摘要： A linear biodevice is provided with fibers that have a curved portion in its cross section approximately perpendicular to the longitudinal direction and approximately spherical adhesive cells that adhere to the periphery of the fiber. A membrane-like biodevice is provided with a sheet that has an opening and approximately spherical adhesive cells that adhere to the opening. A curved portion is present in the cross section on an inner edge of the opening. A bioreactor is provided with a membrane-like biodevice in which spherical adhesive cells grow densely so as to close the openings of the sheet.
摘要翻译：线性生物装置设置有在其横截面中具有大致垂直于纵向方向的弯曲部分的纤维和粘附到纤维周边的近似球形的粘合剂细胞。膜状生物器件设置有具有开口和粘附到开口的近似球形的粘合剂细胞的片材。在开口的内边缘上的横截面中存在弯曲部分。生物反应器设置有膜状生物器官，其中球形粘合剂细胞密集生长以封闭片材的开口。

43. 发明授权

US07569388B2 Plants having an enhanced amino acid content, plants having an enhanced nitrogen content, plants tolerant to nitrogen deficiency, and methods for producing them 有权
标题翻译：具有增强的氨基酸含量的植物，具有增强的氮含量的植物，耐氮缺乏的植物及其生产方法
公开(公告)号：US07569388B2
公开(公告)日：2009-08-04
申请号：US10360634
申请日：2003-02-10
申请人： Shuichi Yanagisawa , Tetsuya Miwa , Ai Akiyama , Hiroaki Kisaka
发明人： Shuichi Yanagisawa , Tetsuya Miwa , Ai Akiyama , Hiroaki Kisaka
IPC分类号： A01H1/00 , C07H21/04 , C07K14/415 , C12N15/00
CPC分类号： C07K14/415 , C12N15/8251
摘要： The object of the present invention is to provide a transformed plant having an enhanced amino acid content, a progeny of the plant, seeds of the plant and a method for producing the plant. The present invention provides a transformed plant having a gene encoding DNA binding protein of Dof family and also having a free amino acid content higher than that of naturally occurring plants of the same species cultivated under the same conditions. The present invention also provides a method of producing a transformed plant having the free amino acid content higher than that of naturally occurring plants of the same species cultivated under the same conditions by introducing a gene encoding DNA binding protein of Dof family into the plant to express the gene in the plant body.
摘要翻译：本发明的目的是提供具有增强的氨基酸含量的转化植物，植物的后代，植物的种子和用于生产植物的方法。本发明提供了具有编码Dof家族的DNA结合蛋白的基因的转化植物，并且其游离氨基酸含量高于在相同条件下栽培的相同种类的天然植物的游离氨基酸含量。本发明还提供了通过将编码Dof家族的DNA结合蛋白的基因导入植物来表达在相同条件下培养的相同种类的天然植物的游离氨基酸含量高的转化植物的生产方法，以表达植物体内的基因。

44. 发明申请

US20060240547A1 Linear and membrane-like biodevices and bioreactors 失效
标题翻译：线性和膜状生物装置和生物反应器
公开(公告)号：US20060240547A1
公开(公告)日：2006-10-26
申请号：US11169910
申请日：2005-06-30
申请人： Satoshi Konishi , Tetsuya Miwa
发明人： Satoshi Konishi , Tetsuya Miwa
IPC分类号： C12N5/00 , C12M1/12 , C12M3/06
CPC分类号： C12M25/10
摘要： A linear biodevice is provided with fibers that have a curved portion in its cross section approximately perpendicular to the longitudinal direction and approximately spherical adhesive cells that adhere to the periphery of the fiber. A membrane-like biodevice is provided with a sheet that has an opening and approximately spherical adhesive cells that adhere to the opening. A curved portion is present in the cross section on an inner edge of the opening. A bioreactor is provided with a membrane-like biodevice in which spherical adhesive cells grow densely so as to close the openings of the sheet.
摘要翻译：线性生物装置设置有在其横截面中具有大致垂直于纵向方向的弯曲部分的纤维和粘附到纤维周边的近似球形的粘合剂细胞。膜状生物器件设置有具有开口和粘附到开口的近似球形的粘合剂细胞的片材。在开口的内边缘上的横截面中存在弯曲部分。生物反应器设置有膜状生物器官，其中球形粘合剂细胞密集生长以封闭片材的开口。

45. 发明授权

US07086363B2 Spark plug and ignition apparatus using same 有权
标题翻译：火花塞和点火装置使用相同
公开(公告)号：US07086363B2
公开(公告)日：2006-08-08
申请号：US10926003
申请日：2004-08-26
申请人： Tetsuya Miwa , Tohru Yoshinaga , Keiji Kanao , Shinichi Okabe
发明人： Tetsuya Miwa , Tohru Yoshinaga , Keiji Kanao , Shinichi Okabe
IPC分类号： F02P1/00
CPC分类号： H01T13/32 , H01T13/20 , H01T13/39
摘要： A power-saving type of ignition apparatus is provided with a spark plug of which electrodes (30, 40) are regulated in their shapes to lower an amount of energy required for its ignition. To face to a tip (31) of a center electrode (30) extending from a mounting bracket (10), a cylindrical protrusion (41) is built on one surface (43) of an earth electrode (40). The protrusion extends toward the center electrode to form a discharge gap (50) between the protrusion and the tip of the center electrode. Both of the tip of the center electrode and the protrusion of the earth electrode are 2.3 [mm] or less in each diameter (D1, D2), thus an amount of energy required for ignition being limited to 17 [mJ] or less.
摘要翻译：节电型点火装置设置有火花塞，其电极（30,40）的形状被调节以降低其点火所需的能量。为了面对从安装支架（10）延伸的中心电极（30）的尖端（31），在接地电极（40）的一个表面（43）上形成有圆筒状突起（41）。突出部朝向中心电极延伸，以在突起和中心电极的尖端之间形成放电间隙（50）。中心电极的尖端和接地电极的突起在每个直径（D 1，D 2）为2.3 [mm]以下，因此点火所需的能量量被限制在17 [mJ]以下。

46. 发明申请

US20060090219A1 Methods for producing plants with improved growth under nitrogen-limited conditions 审中-公开
标题翻译：在氮限制条件下生长具有改善生长的植物的方法
公开(公告)号：US20060090219A1
公开(公告)日：2006-04-27
申请号：US11302262
申请日：2005-12-14
申请人： Hiroaki Kisaka , Tetsuya Miwa , Ai Akiyama
发明人： Hiroaki Kisaka , Tetsuya Miwa , Ai Akiyama
IPC分类号： A01H1/00 , C12N15/82
CPC分类号： C12N15/8261 , Y02A40/146
摘要： The present invention provides a method of producing a plant which exhibits improved growth and/or yield under reduced nitrogen conditions, that is, under cultivation conditions where nitrogen is limited as compared to ordinary cultivation conditions, by increasing 2-OG content in plants. Particularly, the present invention provides a method of producing a plant which exhibits improved growth and/or yield under conditions where nitrogen is reduced, that is, under cultivation conditions where nitrogen is limited as compared to ordinary cultivation conditions, by introducing a GDH gene or ECASPC gene into plants and expressing the transgene GDH or ECAPS in the plants, which results in increased 2-OG, or by spraying proline on the leaves of plants to increase the 2-OG content, thereby enhancing the incorporation of nitrogen or metabolic activity of plants. Also provided is a method of cultivating such plants under nitrogen-limited conditions.
摘要翻译：本发明提供一种生产植物的方法，其在减少的氮条件下，即通过增加植物中2-OG含量，在与普通培养条件相比限制氮的培养条件下，其生长和/或产量改善。特别地，本发明提供一种生产植物的方法，该方法在氮的还原条件下，即在与普通培养条件相比限制的培养条件下，通过引入GDH基因或 ECASPC基因植入植物中并在植物中表达转基因GDH或ECAPS，其导致增加的2-OG，或通过在植物叶上喷洒脯氨酸以增加2-OG含量，从而增强氮的掺入或代谢活性植物。还提供了在氮限制条件下培养这样的植物的方法。

47. 发明申请

US20050052562A1 Solid-state image pickup device 有权
标题翻译：固态图像拾取装置
公开(公告)号：US20050052562A1
公开(公告)日：2005-03-10
申请号：US10920382
申请日：2004-08-18
申请人： Ryu Shimizu , Mitsuru Okigawa , Tetsuya Miwa , Hayato Nakashima
发明人： Ryu Shimizu , Mitsuru Okigawa , Tetsuya Miwa , Hayato Nakashima
IPC分类号： H01L27/14 , H01L27/146 , H04N5/225 , H04N5/335
CPC分类号： H04N5/335 , H01L27/14627 , H01L27/14685
摘要： A solid-state image pickup device improvable in photosensitivity also in the vicinity of an end of a substrate is provided. This solid-state image pickup device comprises a photodetection part formed on a substrate, a color filter layer and a lens, formed between the substrate and the color filter for condensing light on the photodetection part, having a lens center deviated from the center of the photodetection part by a prescribed distance.
摘要翻译：提供了在基片的端部附近也可以在光敏性方面提高的固态摄像装置。该固态摄像装置包括：形成在基板和滤色片之间的光检测部，形成在基板和滤光片之间，用于将光聚焦在光检测部上，其透镜中心偏离光电检测部分指定距离。

48. 发明授权

US06694050B2 Image processing method and image input device, control device, image output device and image processing system employing same 失效
标题翻译：图像处理方法和图像输入装置，控制装置，图像输出装置以及使用它的图像处理系统
公开(公告)号：US06694050B2
公开(公告)日：2004-02-17
申请号：US09813868
申请日：2001-03-22
申请人： Junji Hiraishi , Tetsuya Miwa , Hitoshi Nakamura , Kiyoshi Imai , Kouichi Ohmae , Shinya Sonoda , Souichi Matsushita , Minoru Inada
发明人： Junji Hiraishi , Tetsuya Miwa , Hitoshi Nakamura , Kiyoshi Imai , Kouichi Ohmae , Shinya Sonoda , Souichi Matsushita , Minoru Inada
IPC分类号： G06K900
CPC分类号： H04N1/00846 , H04N1/00843 , H04N1/00848 , H04N1/00851 , H04N1/00867 , H04N1/00875 , H04N1/0088
摘要： To reliably and simply prevent the output of specified image data, a unit to detect a pattern is installed in one of the devices in a system consisting of an image scanner 1, control device 2 (a personal computer) and printer 3 which are connected consecutively. This pattern detection unit contains a unit which detects a specified pattern from the mass of image data representing a document. Once this pattern has been found, either the subsequent image data are altered before being output or output of the image data is interrupted. When the system starts up, the control unit for each device checks whether that device, or one of the other devices in the system, has a pattern detection unit as described above. If it determines that no device has such a unit, it halts operation.
摘要翻译：为了可靠且简单地防止指定图像数据的输出，在由连续连接的图像扫描仪1，控制装置2（个人计算机）和打印机3构成的系统的一个装置中安装检测图案的单元。该图案检测单元包含从表示文档的图像数据的质量检测指定图案的单元。一旦发现了这种模式，在图像数据的输出或输出被中断之前，随后的图像数据被改变。当系统启动时，每个设备的控制单元检查该设备或系统中其他设备之一是否具有如上所述的模式检测单元。如果确定没有设备具有这样的单元，则停止操作。

49. 发明授权

US06437028B1 Crosslinkable elastic copolymer composition 有权
标题翻译：可交联弹性共聚物组合物
公开(公告)号：US06437028B1
公开(公告)日：2002-08-20
申请号：US09673669
申请日：2000-11-07
申请人： Tetsuya Miwa , Takeo Kaneko , Masayuki Saito , Junichi Nakamura
发明人： Tetsuya Miwa , Takeo Kaneko , Masayuki Saito , Junichi Nakamura
IPC分类号： C08K548
CPC分类号： C08K3/22 , C08K5/50 , C08L27/12
摘要： An elastic copolymer composition which contains a copolymer having a content of polymer units based on vinylidene fluoride as low as from 0.5 to 15 mol %, which is satisfactory in polyol vulcanizability and which gives a vulcanizate excellent in heat resistance and oil resistance. This composition comprises a tetrafluoroethylene (35 to 65 mol %)/propylene (20 to 50 mol %)/vinylidene fluoride (0.5 to 15 mol %) copolymer, an organic quaternary ammonium hydroxide or a salt of an organic quaternary phosphonium compound with a nitrogen-containing heterocyclic compound, an organic polyhydroxy compound, and a metal oxide or a metal hydroxide, and optionally contains an amine compound.
摘要翻译：一种弹性共聚物组合物，其含有共聚物，所述共聚物的聚偏二氟乙烯含量低至0.5〜15摩尔％，多元醇硫化性良好，耐硫性优异，耐油性优异。该组合物包含四氟乙烯（35〜65摩尔％）/丙烯（20〜50摩尔％）/偏二氟乙烯（0.5〜15摩尔％）共聚物，有机季铵氢氧化物或有机季鏻化合物与氮含有杂环化合物，有机多羟基化合物，金属氧化物或金属氢氧化物，任选地含有胺化合物。

50. 发明授权

US06303359B1 DNA molecule encoding new aminopeptidase, and method of producing the aminopeptidase 失效
标题翻译：编码新氨基肽酶的DNA分子，以及氨基肽酶的制备方法
公开(公告)号：US06303359B1
公开(公告)日：2001-10-16
申请号：US09525046
申请日：2000-03-14
申请人： Daiki Ninomiya , Tetsuya Miwa , Minao Asano , Nami Nakamura , Noriki Nio
发明人： Daiki Ninomiya , Tetsuya Miwa , Minao Asano , Nami Nakamura , Noriki Nio
IPC分类号： C12N948
CPC分类号： C12N9/48
摘要： Disclosed are cDNA encoding a new aminopeptidase derived from germinated soybeans, a recombinant expression vector containing the DNA, a transformant obtained by the transformation with the expression vector, and a method of producing an aminopeptidase by culturing the transformed product. According to the present invention, a cDNA can be obtained encoding the amino acid sequence of aminopeptidase GX suitable for producing a highly hydrolized product from a starting material containing a protein and peptide having a high acidic amino acid content. Using the cDNA, a recombinant aminopeptidase can be mass-produced with E. coli or the like. By using the cDNA, it is also possible to produce aminopeptidase GX by using a host other than E. coli. In addition, hydrohized products having high glutamic acid content and aspartic acid content and also excellent seasoning properties can be obtained from soybean protein by combining GX thus produced with protease D3 and leucine aminopeptidase DLAP.
摘要翻译：公开了编码来自发芽大豆的新氨基肽酶的cDNA，含有DNA的重组表达载体，通过用表达载体转化获得的转化体，以及通过培养转化产物来生产氨基肽酶的方法。根据本发明，可以获得编码氨肽酶GX的氨基酸序列的cDNA，该氨基酸序列适于从含有高酸性氨基酸含量的蛋白质和肽的原料制备高度水解的产物。使用该cDNA，重组氨基肽酶可以用大肠杆菌等大量生产。通过使用cDNA，也可以通过使用除大肠杆菌以外的宿主产生氨基肽酶GX。此外，通过将由此产生的GX与蛋白酶D3和亮氨酸氨基肽酶DLAP组合，可以从大豆蛋白获得具有高谷氨酸含量和天冬氨酸含量以及天冬氨酸含量高的水解产物。

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