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    • 42. 发明申请
    • PROCESS FOR CONCENTRATING AND PROCESSING FLUID SAMPLES
    • 浓缩和加工流体样品的方法
    • WO2010002552A1
    • 2010-01-07
    • PCT/US2009/046755
    • 2009-06-09
    • GENERAL ELECTRIC COMPANYBOYETTE, ScottXU, RongLUO, JingXU, WeiqingCHEN, JingXIAO, WeiminCHEN, Tong
    • BOYETTE, ScottXU, RongLUO, JingXU, WeiqingCHEN, JingXIAO, WeiminCHEN, Tong
    • B01D61/00C12Q1/04C12Q1/24G01N33/487
    • C12Q1/24
    • A method of treating a liquid sample having microbiological target species therein to concentrate the species and collect lysate is disclosed. The liquid sample comprises non-target microbiological particles, inorganic particles, and microbiological target species. The liquid is passed through a prefilter medium to allow the target species to pass through as filtrate and retain non-target microbiological products and inorganic particles thereon. The filtrate is contacted with a main filtration medium adapted to retain the target species thereon as retentate. The retentate is lysed to form a lysate containing target material that was enveloped within the microbiological target species. The microbiological target species may comprise cellular organisms such as bacteria, algae, fungi, prokaryotes, etc. or viruses. Target materials comprise intracellular nucleic acids, or in the case of viral sampling, nucleic acids encased within the protein sheath or coating of the virus.
    • 公开了一种处理其中具有微生物靶物种的液体样品以浓缩物种并收集裂解物的方法。 液体样品包括非目标微生物颗粒,无机颗粒和微生物目标物种。 使液体通过预过滤介质,使目标物质作为滤液通过,并在其上保留非目标微生物产物和无机颗粒。 将滤液与适于将其上保留目标物质的主过滤介质接触,作为保留物。 将渗余物溶解以形成包含在微生物目标物种内的目标物质的裂解物。 微生物靶物种可以包括细菌生物,如细菌,藻类,真菌,原核生物等,或病毒。 目标材料包括细胞内核酸,或在病毒取样的情况下,包裹在病毒的蛋白质鞘或涂层内的核酸。