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41. 发明授权

US5998588A Interactive molecular conjugates 失效
标题翻译：交互分子共轭物
公开(公告)号：US5998588A
公开(公告)日：1999-12-07
申请号：US697904
申请日：1996-08-30
申请人： Allan S. Hoffman , Patrick S. Stayton
发明人： Allan S. Hoffman , Patrick S. Stayton
IPC分类号： G01N33/53 , A61K31/70 , A61K31/715 , A61K38/00 , A61K38/43 , A61K39/00 , A61K39/395 , A61K45/00 , A61K47/48 , C07K1/16 , C07K17/00 , C08F8/36 , C08F20/54 , G01N33/532 , G01N33/566 , C07K17/08 , A61K39/385 , A61K3/74
CPC分类号： B82Y5/00 , A61K47/48369 , A61K47/48723 , A61K47/48753 , G01N33/532
摘要： The combination of the capabilities of stimuli-responsive components such as polymers and interactive molecules to form site-specific conjugates which are useful in a variety of assays, separations, processing, and other uses is disclosed. The polymer chain conformation and volume can be manipulated through alteration in pH, temperature, light, or other stimuli. The interactive molecules can be biomolecules like proteins or peptides, such as antibodies, receptors, or enzymes, polysaccharides or glycoproteins which specifically bind to ligands, or nucleic acids such as antisense, ribozymes, and aptamers, or ligands for organic or inorganic molecules in the environment or manufacturing processes. The stimuli-responsive polymers are coupled to the recognition biomolecules at a specific site so that the polymer can be manipulated by stimulation to alter ligand-biomolecule binding at an adjacent binding site, for example, the biotin binding site of streptavidin, the antigen-binding site of an antibody or the active, substrate-binding site of an enzyme. Binding may be completely blocked (i.e., the conjugate acts as an on-off switch) or partially blocked (i.e., the conjugate acts as a rheostat to partially block binding or to block binding only of larger ligands). Once a ligand is bound, it may also be ejected from the binding site by stimulating one (or more) conjugated polymers to cause ejection of the ligand and whatever is attached to it. Alternatively, selective partitioning, phase separation or precipitation of the polymer-conjugated biomolecule can be achieved through exposure of the stimulus-responsive component to an appropriate environmental stimulus.
摘要翻译：公开了可用于各种测定，分离，加工和其它用途的刺激响应组分如聚合物和交互分子的能力的组合以形成位点特异性缀合物。聚合物链构象和体积可以通过改变pH，温度，光或其他刺激来操纵。交互分子可以是诸如蛋白质或肽的生物分子，例如抗体，受体或酶，特异性结合配体的多糖或糖蛋白，或核酸如反义，核酶和适体，或者在有机或无机分子中的配体环境或制造过程。刺激响应聚合物与特定位点处的识别生物分子偶联，使得可以通过刺激来操纵聚合物，以改变相邻结合位点处的配体 - 生物分子结合，例如链霉抗生物素蛋白的生物素结合位点，抗原结合抗体的位点或酶的活性，底物结合位点。结合可能被完全阻断（即，共轭体作为开 - 关开关）或部分封闭（即共轭物作为变阻器部分阻断结合或仅阻断较大配体的结合）。一旦配体结合，它也可以通过刺激一种（或多种）共轭聚合物从结合位点喷射，引起配体的排出以及附着于其上的任何物质。或者，可以通过将刺激响应组分暴露于合适的环境刺激来实现聚合物共轭生物分子的选择性分配，相分离或沉淀。

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