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31. 发明申请

US20040106193A1 Novel adenoviral vector and methods for making and using the same 有权
标题翻译：新型腺病毒载体及其制备和使用方法
公开(公告)号：US20040106193A1
公开(公告)日：2004-06-03
申请号：US09930832
申请日：2001-08-15
发明人： Mark A. Kay , Hiroyuki Mizuguchi
IPC分类号： C12P021/06 , A61K039/12 , A61K039/23 , A61K039/235 , C12N015/00 , C12N015/09 , C12N015/63 , C12N015/70 , C12N015/74
CPC分类号： C12N15/86 , A61K48/00 , C07K14/8125 , C12N2710/10343 , C12N2800/80
摘要： In vitro methods for making a recombinant adenoviral genome, as well as kits for practicing the same and the recombinant adenovirus vectors produced thereby, are provided. In the subject methods, the subject genomes are prepared from first and second vectors. The first vector includes an adenoviral genome having an E region deletion and three different, non-adenoviral restriction endonuclease sites located in the E region. The second vector is a shuttle vector and includes an insertion nucleic acid flanked by two of the three different non-adenoviral vectors present in the first vector. Cleavage products are prepared from the first and second vectors using the appropriate restriction endonucleases. The resultant cleavage products are then ligated to produce the subject recombinant adenovirus genome. The subject adenoviral genomes find use in a variety of application, including as vectors for use in a variety of applications, including gene therapy.
摘要翻译：提供了制备重组腺病毒基因组的体外方法，以及用于实施其的试剂盒和由此产生的重组腺病毒载体。在本发明方法中，从第一和第二载体制备受试者基因组。第一载体包括具有E区缺失的腺病毒基因组和位于E区中的三个不同的非腺病毒限制性内切核酸酶位点。第二载体是穿梭载体，并且包含侧翼于第一载体中存在的三种不同非腺病毒载体中的两个的插入核酸。使用适当的限制性内切核酸酶从第一和第二载体制备裂解产物。然后将所得裂解产物连接以产生本发明的重组腺病毒基因组。主题腺病毒基因组可用于各种应用，包括用于各种应用的载体，包括基因治疗。

32. 发明申请

US20040091966A1 Polypeptide regulation by conditional inteins 审中-公开
标题翻译：多肽调节条件内含肽
公开(公告)号：US20040091966A1
公开(公告)日：2004-05-13
申请号：US10441147
申请日：2003-05-19
发明人： Martin Zeidler , Norbert Perrimon
IPC分类号： C12P021/02 , C07H021/04 , C12P021/06 , C07K014/47 , C12N015/85
CPC分类号： C12N15/67 , C12N9/104
摘要： The present invention relates to methods and reagents for the regulation of a target polypeptide bioactivity by controlled self-excision of an intein.
摘要翻译：本发明涉及通过受体自身切除内含肽调节靶多肽生物活性的方法和试剂。

33. 发明申请

US20040091957A1 Methods for making antibody fragments and compositions resulting therefrom 审中-公开
标题翻译：制备抗体片段的方法及由此产生的组合物
公开(公告)号：US20040091957A1
公开(公告)日：2004-05-13
申请号：US10701887
申请日：2003-11-04
申请人： Zyomyx, Inc.
发明人： Steffen Nock , David S. Wilson , Jiangchun Wu
IPC分类号： C12P021/06 , C07K016/18
CPC分类号： C07K16/065 , C07K2317/41 , C07K2317/54 , Y10S530/866
摘要： Improved methods for making antibody fragments, preferably F(abnull)2 fragments from various classes and subclasses of antibodies is described. Pretreatment of antibodies with deglycosylases or cellular inhibition of glycosylation during expression, yields antibodies having improved susceptibility towards protease cleavage, preferably pepsinolysis, which yields F(abnull)2 antibody fragments. Compositions resulting from such methods are further disclosed.
摘要翻译：描述了用于制备来自抗体的各种类型和亚类的抗体片段，优选F（ab'）2片段的改进方法。在表达过程中用去糖基化酶预处理抗体或细胞抑制糖基化，产生具有改善的对蛋白酶切割的易感性的抗体，优选为肽溶解，其产生F（ab'）2抗体片段。进一步公开了由这些方法得到的组合物。

34. 发明申请

US20040086959A1 Method of preparing polypeptides in cell-free system and device for its realization 审中-公开
标题翻译：在无细胞系统中制备多肽的方法及其实现的装置
公开(公告)号：US20040086959A1
公开(公告)日：2004-05-06
申请号：US10307122
申请日：2002-11-27
发明人： Sergey Vladimirovich Biryukov , Peter Nikolayevich Simonenko , Alena Simonenko , Vladimir Anatolievich Shirokov , Sergey Gennadievich Mayorov , Alexander Sergeyevich Spirin
IPC分类号： C12P021/06
CPC分类号： C12M23/34 , C12M23/02 , C12M25/16 , C12M29/04 , C12M29/16 , C12M29/18 , C12P21/02
摘要： The present invention provides a method for synthesis of polypeptides in a cell-free system. In this method, products of synthesis are separated into a low molecular weight fraction and a high molecular weight fraction, with the high molecular weight fraction containing the target polypeptide. The method calls for the removal of at least a portion of the low molecular weight fraction via a porous barrier.
摘要翻译：本发明提供了在无细胞系统中合成多肽的方法。在该方法中，将合成产物分离成低分子量级分和高分子量级分，高分子量级分含有靶多肽。该方法要求通过多孔屏障除去至少一部分低分子量馏分。

35. 发明申请

US20040082027A1 Conjugate of a tissue non-specific alkaline phosphatase and dextran, process for its production and use thereof 失效
标题翻译：组织非特异性碱性磷酸酶和葡聚糖的缀合物，其生产和使用的方法
公开(公告)号：US20040082027A1
公开(公告)日：2004-04-29
申请号：US10624154
申请日：2003-07-21
发明人： Bernd Schneidinger , Thomas Meier , Rainer Schmuck , Zhixin Shao
IPC分类号： C12P021/06 , C12N009/16
CPC分类号： G01N33/96 , C12N9/14 , C12Q1/42
摘要： A conjugate of a tissue non-specific alkaline phosphatase (tns-AP) and dextran which can be obtained by reacting unglycosylated tns-AP with activated dextran by incubation in aqueous solution, stopping the reaction and isolating the conjugate from the solution. The conjugate obtained in this manner is suitable as a standard for the determination of alkaline phosphatase.
摘要翻译：组织非特异性碱性磷酸酶（tns-AP）和葡聚糖的缀合物，其可以通过在水溶液中温育而使未糖基化的tns-AP与活化的葡聚糖反应而获得，停止反应并从溶液中分离缀合物。以这种方式获得的缀合物适合作为碱性磷酸酶测定的标准。

36. 发明申请

US20040082026A1 Interferon alpha: remodeling and glycoconjugation of interferon alpha 有权
标题翻译：干扰素α：干扰素α的重塑和糖结合
公开(公告)号：US20040082026A1
公开(公告)日：2004-04-29
申请号：US10411049
申请日：2003-04-09
申请人： Neose Technologies, Inc.
发明人： Shawn DeFrees , David Zopf , Robert Bayer , Caryn Bowe , David Hakes , Xi Chen
IPC分类号： C12P021/06 , C07K014/54
CPC分类号： C07K1/006 , A61K38/00 , C07K1/1077 , C07K1/13 , C07K9/00 , C07K14/505 , Y02A50/472
摘要： The invention includes a multitude of methods and compositions for remodeling a peptide molecule, including the addition or deletion of one or more glycosyl groups to a peptide, and/or the addition of a modifying group to a peptide.
摘要翻译：本发明包括用于重组肽分子的多种方法和组合物，包括向肽添加或缺失一个或多个糖基，和/或向肽添加修饰基团。

37. 发明申请

US20040081968A1 Method and mixture for curing autoimmune disease 审中-公开
标题翻译：治疗自身免疫性疾病的方法和混合物
公开(公告)号：US20040081968A1
公开(公告)日：2004-04-29
申请号：US10281862
申请日：2002-10-28
发明人： Harvey Lang
IPC分类号： C12Q001/68 , A61K031/70 , A01N043/04 , C12P021/06 , A61K039/12
CPC分类号： A61K39/12 , A61K2039/545 , A61K2039/58 , A61K2039/70 , C12N2710/16234 , C12N2710/16634 , C12N2710/16734 , C12N2760/16034 , C12N2770/32334
摘要： Method and mixture for curing autoimmune disease. An Applied Kinesiology technique used to desensitize a patient to allergies is used to desensitize a patient to seven basic viruses. Desensitizing the patient to the seven basic viruses cures autoimmune disease. A homeopathic mixture of the seven basic viruses is used for optimal performance of the curative method.
摘要翻译：治疗自身免疫性疾病的方法和混合物用于使患者过敏的应用运动机能学技术用于使患者脱敏为七种基本病毒。使患者脱敏至七种基本病毒治愈自身免疫性疾病。七种基本病毒的顺势疗法混合物用于治疗方法的最佳表现。

38. 发明申请

US20040077038A1 Methods for production of proteins in host cells 审中-公开
公开(公告)号：US20040077038A1
公开(公告)日：2004-04-22
申请号：US10714212
申请日：2003-11-13
发明人： Mark Donnelly , Andrzej Joachimiak
IPC分类号： C12P021/06 , C12N009/48 , C12N001/21 , C12N015/74
CPC分类号： C12N15/70
摘要： The present invention provides methods for the production of proteins, particularly toxic proteins, in host cells. The invention provides methods which use a fusion protein comprising a chaperonin binding domain in host cells induced or regulated to have increased levels of chaperonin which binds the chaperonin binding domain.

39. 发明申请

US20040072282A1 Synthesis of membrane proteins 审中-公开
标题翻译：膜蛋白的合成
公开(公告)号：US20040072282A1
公开(公告)日：2004-04-15
申请号：US10602265
申请日：2003-06-23
申请人： Sarnoff Corporation
发明人： Frank Guarnieri , John Kulp III , William Chiang
IPC分类号： C12P021/06 , C07K014/47
CPC分类号： C07K1/026
摘要： Methods for chemical ligation and synthesis of peptides and polypeptides including membrane polypeptides and hydrophobic peptides and polypeptides or membrane polypeptide domains in a multiphase solvent mixture comprising at least two component solvents; compositions produced using these methods and assays performed using these compositions.
摘要翻译：包含至少两种组分溶剂的多相溶剂混合物中化学连接和合成肽和多肽的方法，包括膜多肽和疏水肽和多肽或膜多肽结构域; 使用这些方法制备的组合物和使用这些组合物进行的测定。

40. 发明申请

US20040063911A1 Protein remodeling methods and proteins/peptides produced by the methods 有权
标题翻译：蛋白质重塑方法和蛋白质/肽通过方法生产
公开(公告)号：US20040063911A1
公开(公告)日：2004-04-01
申请号：US10411026
申请日：2003-04-09
申请人： Neose Technologies, Inc.
发明人： Shawn DeFrees , David Zopf , Robert Bayer , David Hakes , Xi Chen
IPC分类号： C12P021/06 , C07K014/54
CPC分类号： C07K1/006 , C07K1/1077 , C07K1/13 , C07K9/00 , C07K14/505 , C07K2319/00 , Y02A50/472
摘要： The invention includes methods and compositions for remodeling a peptide molecule, including the addition or deletion of one or more glycosyl groups to a peptide, and/or the addition of a modifying group to a peptide.
摘要翻译：本发明包括用于重组肽分子的方法和组合物，包括向肽添加或缺失一个或多个糖基，和/或向肽中加入修饰基团。

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