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31. 发明专利

JP3829157B2 Gibberellin 2β hydroxylase gene and its use from rice 有权
公开(公告)号：JP3829157B2
公开(公告)日：2006-10-04
申请号：JP36589999
申请日：1999-12-24
申请人：独立行政法人理化学研究所 , 独立行政法人農業生物資源研究所
发明人：正智小林 , 信松岡 , 宥司田中 , 暁明萱野
IPC分类号： C12N15/09 , A01H5/00 , C07K14/415 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/02 , C12N15/53 , C12N15/82 , C12P17/18 , C12P21/02
CPC分类号： C12N9/0071 , C12N15/8297
摘要： Novel GA2 beta -hydroxylase genes were successfully isolated from rice. In addition, plants whose plant type has been modified compared with their wild type counterparts, were successfully constructed via these genes.

32. 发明公开

KR1020010090954A 수박의 종자 발생시 종피특이적으로 유전자의 발현을조절하는 Ｃｖ２０ｏｘ유전자 프로모터 无效
标题翻译： CV20OX基因启动子在番茄发育过程中特异性调节基因表达
公开(公告)号：KR1020010090954A
公开(公告)日：2001-10-22
申请号：KR1020000018483
申请日：2000-04-08
申请人： 주식회사 디비하이텍
发明人： 안진흥 , 강홍규 , 전성훈 , 김준열 , 정기환 , 박용주 , 이상엽
IPC分类号： C12N15/63
CPC分类号： C12N9/0071 , C12N15/8234 , C12N15/8287 , C12N15/8297
摘要： PURPOSE: Cv20ox gene promotor is provided which is separated from Citrullus lanatus seeds on a developmental stage and regulates gene expression spornioderm-specifically. CONSTITUTION: Cv20ox gene promotor is expressed in an integumental tissue of Citrullus lanatus seeds on a developmental stage. Cv20ox gene promotor regulating the gene expression spornioderm-specifically is prepared by following steps of: separating cDNA of Cv20ox gene which codes Gibberellin(GA)20-oxidant Cv20ox; separating Cv20ox gene promotor from total genome DNA using 700bp of 5' upstream region of Cv20ox promotor as PCR cloning kit; and measuring the activity of Cv20ox gene promotor by histochemical detection using expression vector pGA2118. Wherein, The expression vector pGA2118 is prepared by the steps of: cutting vector derivative pGA1230 including GUS reporter gene, with HindIII and PstI; and inserting 0.7kb promotor segment of Cv20ox obtained from a recombinant vector pGA2044.
摘要翻译：目的：提供Cv20ox基因启动子，在发育阶段与柑橘种子分离，特异性调节基因表达。构成：Cv20ox基因启动子在发育阶段在柑橘种子的皮肤组织中表达。通过以下步骤制备Cv20ox基因启动子：通过以下步骤分离编码赤霉素（GA）20氧化剂Cv20ox的Cv20ox基因的cDNA; 使用700bp的Cv20ox启动子的5'上游区域作为PCR克隆试剂盒将Cv20ox基因启动子与总基因组DNA分离; 并用表达载体pGA2118通过组织化学检测来测定Cv20ox基因启动子的活性。其中，通过以下步骤制备表达载体pGA2118：用HindIII和PstI切割包含GUS报告基因的载体衍生物pGA1230; 并插入从重组载体pGA2044获得的0.7kb的Cv20ox启动子片段。

33. 发明公开

KR1020020093028A 수박의 종자 발생시 종피 특이적으로 유전자의 발현을조절하는 Ｃｖ20ｏｘＰ 유전자 프로모터 및 이를 이용한씨 없는 식물체의 제조방법 无效
标题翻译：促进剂CV20OXP调节GIBBERELLIN 20氧化酶基因在开发水稻种子中的综合特异性表达和使用促进剂产生无籽水果的方法
公开(公告)号：KR1020020093028A
公开(公告)日：2002-12-12
申请号：KR1020027013435
申请日：2000-10-09
申请人： 주식회사 디비하이텍
发明人： 안진흥 , 강홍규 , 전성훈 , 김준열 , 정기환 , 박용주 , 이상엽
IPC分类号： C12N15/53
CPC分类号： C12N9/0071 , C12N15/8234 , C12N15/8287 , C12N15/8297
摘要： PURPOSE: Provided are a testa specific gene, which is isolated from the developing seed of Citrullus lanatus, a promoter and a method for generating seedless fruits by using the promoter of the said testa specific gene. CONSTITUTION: The Cv20ox gene, gibberellin 20-oxidase(GA 20-oxidase) gene showing the tissue-specific expression pattern, is strongly expressed in the integumental tissue at seed development of watermelon and is weakly expressed in the inner tissue of seed. The promoter regulates the integument-specific expression of the gene and the method for generating seedless fruits is characterized by introducing plant growth regulation gene ligated to the promoter and regulating the overexpression of plant growth regulation composition in the testa.
摘要翻译：目的：提供一种睾丸特异性基因，其通过使用所述睾丸特异性基因的启动子从柑橘柑橘的发育种子，启动子和产生无核果实的方法中分离。构成：显示组织特异性表达模式的赤霉素20-氧化酶（GA 20-氧化酶）基因的Cv20ox基因在西瓜种子发育中在皮肤组织中强烈表达，并且在种子的内部组织中弱表达。启动子调节基因的特异性表达，产生无核果实的方法的特征在于引入连接到启动子的植物生长调节基因并调节试验中植物生长调节组成的过表达。

34. 发明公开

KR1020120078950A 식물 뿌리 성장 조절용 형질전환 식물 및 그 방법 有权
标题翻译：用于调节植物根生长的转基因植物及其方法
公开(公告)号：KR1020120078950A
公开(公告)日：2012-07-11
申请号：KR1020110000263
申请日：2011-01-03
申请人： 건국대학교 산학협력단
发明人： 임준
IPC分类号： A01H5/00 , C12N15/29 , C12N15/82
CPC分类号： C12N15/8261 , C12N15/8205 , C12N15/8227 , C12N15/8297
摘要： PURPOSE: A transgenic plant for controlling plant root growth is provided to control cell proliferation and cell division. CONSTITUTION: A transgenic plant for controlling plant root growth is produced by transforming a plant with a gene encoding SCARECROW-LIKE'SCL 3 polypeptides. The polypeptide has an amino acid sequence of sequence number 1. The gene encoding the polypeptide has a base of sequence number 2. A method for enhancing plant root growth comprises a step of overexpressing the gene under the control of a Cauliflower mosaic virus 35S promoter. The promoter has a base of sequence number 3.
摘要翻译：目的：提供一种控制植物根生长的转基因植物，以控制细胞增殖和细胞分裂。构成：通过用编码SCARECROW-LIKE'SCL 3多肽的基因转化植物来产生用于控制植物根生长的转基因植物。多肽具有序列号1的氨基酸序列。编码多肽的基因具有序列号2的碱基。用于增强植物根生长的方法包括在花椰菜花叶病毒35S启动子控制下过表达该基因的步骤。启动子具有序列号3的碱基。

35. 发明公开

KR1020090118286A ＳＯＭＮＵＳ 또는 이와 기능적 동등물의 세포내 수준을조절하여 식물의 종자의 발아를 변화시키는 방법 失效
标题翻译：通过控制细胞中SOMNUS或其功能等价物来修饰植物发芽的方法
公开(公告)号：KR1020090118286A
公开(公告)日：2009-11-18
申请号：KR1020080043981
申请日：2008-05-13
申请人： 한국과학기술원
发明人： 최길주 , 김동환
IPC分类号： A01H4/00
CPC分类号： C12N15/8218 , C12N15/113 , C12N15/8205 , C12N15/8206 , C12N15/8293 , C12N15/8297 , C12N2310/14
摘要： PURPOSE: A method for modifying germination of plant seed by controlling SOMNUS or functional equivalent at intracellular level is provided. CONSTITUTION: A method for modifying germination of plant seed by controlling SOMNUS or functional equivalent at intracellular level comprises a step of controlling the amount of ABA and GA by regulating the expression of gene which relates to synthesis or lysis of GA and ABA. When the expression is suppressed, the necessity for light is reduced in germination of seed.
摘要翻译：目的：提供一种通过控制SOMNUS或功能等同于细胞内水平来修饰植物种子发芽的方法。构成：通过控制SOMNUS或在细胞内水平上的功能等价物来修饰植物种子发芽的方法包括通过调节与GA和ABA的合成或裂解有关的基因的表达来控制ABA和GA的量的步骤。当表达被抑制时，种子的发芽减少了光的必要性。

36. 发明公开

KR1020040019016A 식물의 반왜성화에 관여하는 ｓｄ1 유전자 및 그의 이용 有权
标题翻译： Sd1基因参与植物的抗除草和利用
公开(公告)号：KR1020040019016A
公开(公告)日：2004-03-04
申请号：KR1020037016443
申请日：2002-06-07
申请人： 혼다 기켄 고교 가부시키가이샤
发明人： 오카와미호 , 마쓰오카마코토 , 아시카리모토유키
IPC分类号： C12N15/09 , C12N15/63 , C12N5/14 , C12N15/29
CPC分类号： C12N9/0071 , C12N15/8297
摘要： 본 발명자 등은, sd1 유전자가 C20 산화효소 유전자라고 생각하여, 아라비돕시스 C20 산화효소 유전자의 벼 카운터파트의 분리 및 동정을 행하였다. 그 결과, 벼 sd1 유전자는 신규한 C20 산화효소로부터 코드된 유전자라는 것이 판명되었다. 이후 검토의 결과, 그 유전자의 변이가, 식물의 반왜성화을 유도하는 것이 명확하게 되었다. 식물의 sd1 유전자의 이용에 의하여, 유용 농작물 및 관상용 식물 등의 식물의 고수량화 또는 왜성화에 의하여 미적 가치의 부가, 나아가 마커 선발에 의한 고수량화 또는 왜성화된 식물의 효율적 육종 등이 가능하게 될 것으로 크게 기대된다.

37. 发明公开

KR1020000050798A 식물 호르몬 지베렐린의 신호전달 과정에 관여하는 새로운 키나 제 및 그 유전자 失效
标题翻译：与吉非贝因及其基因信号转导相关的激酶
公开(公告)号：KR1020000050798A
公开(公告)日：2000-08-05
申请号：KR1019990000906
申请日：1999-01-15
申请人： 한국과학기술연구원
发明人： 배현숙
IPC分类号： C12N9/12
CPC分类号： C12N9/12 , C12N15/70 , C12N15/8297
摘要： PURPOSE: A kinase related to the signal transduction of gibberellin, the type of phytohormone and a gene encoding the kinase are provided which are useful in regulating physiological function of gibberellin. CONSTITUTION: A kinase, NtDSK1, is isolated from cytoplasm of Nicotin tabacum. The kinase has dual specificity phosphorylating residues of serine, treosine or tyrosine and promotes gene expression regulated by gibberellin or related thereto in the maturation of stamen and the seed thereof, and has amino acid sequence and nucleotide sequence represented by SEQ. ID. NO:1 and SEQ. ID. NO:2, respectively. And plasmid vector pNtDSK1(KCTC 8917P) comprising cDNA gene of the kinase. Consequently a recombinant plant having modified signal transduction of gibberellin is prepared by using E. coli transformed with the plasmid vector pNtDSK1 having kinase genes.
摘要翻译：目的：提供与赤霉素信号转导相关的激酶，植物激素类型和编码激酶的基因，可用于调节赤霉素的生理功能。构成：从Nicotin tabacum的细胞质中分离出一种激酶NtDSK1。该激酶具有丝氨酸，铁蛋白或酪氨酸的双重特异性磷酸化残基，并促进雄蕊及其种子成熟时由赤霉素或与之相关的基因表达，并具有SEQ ID NO：1所示的氨基酸序列和核苷酸序列。 ID。 NO：1和SEQ。 ID。 NO：2。和质粒载体pNtDSK1（KCTC 8917P），其包含激酶的cDNA基因。因此，通过使用用具有激酶基因的质粒载体pNtDSK1转化的大肠杆菌来制备具有修饰的赤霉素信号转导的重组植物。

38. 发明公开

EP2894967A4 WHEAT WITH NEW ALLELES OF RHT-B1 审中-公开
标题翻译：用新的等位基因RHT-B1小麦
公开(公告)号：EP2894967A4
公开(公告)日：2016-05-18
申请号：EP13830254
申请日：2013-08-22
申请人： COMMW SCIENT IND RES ORG , GRAINS RES & DEV CORP
发明人： CHANDLER PETER MICHAEL , HARDING CAROL ANNE
IPC分类号： A01H5/00 , C12N15/29
CPC分类号： A01H5/10 , A01D91/04 , A01F12/30 , A01F12/60 , A01H1/02 , A01H1/04 , A21D13/80 , A23K10/30 , A23L7/109 , A23L7/115 , A23L7/117 , A23L7/198 , A23L23/00 , A23V2002/00 , C07K14/415 , C08B30/046 , C12N15/8261 , C12N15/8297 , C12P7/06 , C12Q1/6895 , Y02A40/146 , Y02E50/17
摘要： The present invention provides a wheat plant comprising an Rht-B1 allele which encodes an Rht-B1 (DELLA) poly-peptide. Grain from a near-isogenic wheat line comprising the dwarfmg Rht-B1c allele was subjected to sodium azide mutagenesis. Plants exhibiting early leaf elongation rates or mature plant height greater than the dwarf parent were selected and the Rht-B1 gene sequenced. This identified 35 mutated alleles of Rht-B1c. Similar methods were also used to identify mutant alleles of the dwarfmg s1n1d allele in barley, where DELLA is encoded by the s1n1 gene.

39. 发明公开

EP2020846A2 COMPOSITIONS FOR SILENCING THE EXPRESSION OF GIBBERELLIN 2-OXIDASE AND USES THEREOF 审中-公开
标题翻译：组合物灭活表达赤霉素2氧化酶和用途，因此
公开(公告)号：EP2020846A2
公开(公告)日：2009-02-11
申请号：EP07736369.5
申请日：2007-05-24
申请人： Ramot at Tel-Aviv University Ltd.
发明人： ALONI, Roni , AVNI, Adi , DAYAN, Jonathan
IPC分类号： A01H5/00 , A01H5/10 , A01H5/04 , C12N5/14 , C12N15/82 , C12N9/02
CPC分类号： C12N15/8261 , C12N9/0071 , C12N15/8297 , Y02A40/146
摘要： The present invention provides an isolated nucleic acid sequence encoding gibberellin 2-oxidase enzyme (GA 2-oxidase) of Hibiscus cannabinus. Furthermore, the present invention provides methods of silencing the expression of GA 2-oxidase gene via RNA interference for increasing fibers in plants, in particular in kenaf.

40. 发明授权

EP1245680B1 GIBBERELLIN 2 BETA-HYDROXYLASE GENES OF RICE AND USES THEREOF 有权
标题翻译：赤霉素2 BETA羟化酶水稻和基因及其用途
公开(公告)号：EP1245680B1
公开(公告)日：2007-05-16
申请号：EP00987655.8
申请日：2000-12-20
申请人： NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES , RIKEN
发明人： TANAKA, Hiroshi, National Instit. of Agrobiol.Scie , KAYANO, Toshiaki, National Instit. of Agrobio.Scie , MATSUOKA, Makoto, Nagoya Univ. BioScience Center , KOBAYASHI, Masatomo, Riken Tsukuba Institute
IPC分类号： C12N15/53
CPC分类号： C12N9/0071 , C12N15/8297
摘要： Novel GA2β-hydroxylase genes are successfully isolated from rice. Also, plants with modified grass types, compared with the wild type plants, are successfully constructed by using these genes.

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