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1. 发明授权

KR100497717B1 브로콜리의 체세포배 배양 방법 및 그를 이용한 브로콜리식물체싹의 대량 생산 방법 失效
标题翻译：培养西兰花体细胞胚的方法和使用该方法大量生产西兰花芽的方法
公开(公告)号：KR100497717B1
公开(公告)日：2005-06-28
申请号：KR1020020066165
申请日：2002-10-29
申请人： 주식회사 디비하이텍
发明人： 박용주 , 윤은정 , 김형태 , 정기환 , 우은택
IPC分类号： A01H4/00
摘要： 본 발명은 브로콜리의 체세포배 배양 방법 및 이를 이용한 브로콜리 식물체싹의 대량 생산 방법에 관한 것으로서, 보다 상세하게는 1) 배를 형성할 수 있는 브로콜리의 조직을 배 유기용 배지에 배양하여 배를 유기시키는 단계; 2) 단계 1의 배를 절단하여 캘러스 형성 배지에 치상한 후 배양하여 캘러스를 형성시키는 단계; 3) 단계 2의 캘러스로부터 배발생 세포를 형성시키는 단계; 및 4) 단계 3의 배발생 세포를 호르몬이 제거된 배지에 옮겨 계대배양을 실시하는 단계를 포함하는 브로콜리의 체세포배 배양 방법 및 이를 이용한 브로콜리 식물체싹의 대량 생산 방법에 관한 것이다. 본 발명의 브로콜리의 체세포배 배양 방법은 종자로부터 얻을 수밖에 없었던 브로콜리의 식물체싹을 체세포를 이용하여 대량 생산하게 함으로써 짧은 시간 내에 간편하게 무한정으로 브로콜리의 식물체싹을 생산할 수 있으며, 상기의 방법으로 생산된 브로콜리의 유식물체는 항암제성분인 설포라판이 다량 함유되어 있으므로 항암용 건강식품 또는 의약품의 원료로써 값싸게 사용될 수 있을 뿐만 아니라, 브로콜리의 형질전환 등과 같은 생명공학 기술에 매우 유용하게 사용될 수 있다.

2. 发明授权

KR100414641B1 형질전환식물체의 생체분석방법 및 그를 이용한 시스템 失效
标题翻译： 형질전환식물체의생체분석방법및그를이용한시스템
公开(公告)号：KR100414641B1
公开(公告)日：2004-01-13
申请号：KR1020000018104
申请日：2000-04-07
申请人： 이춘환 , 주식회사 디비하이텍
发明人： 이춘환 , 김주곤 , 정병철 , 박용주
IPC分类号： G01N33/48
CPC分类号： G01N21/6428
摘要： The present invention relates to a method for visualizing GFP expression in callus, various tissue and organ of the transgenic plants as image and system using the same. The said method needs no other additional genetic product, substrate or cofactor and can detect very simply and quickly GFP expression by using the said system of the present invention consisting of a CCD camera, a light source, band-pass filter and data processing computer, so it provides many advantages for selection of transgenic seeds, for studying of gene expression in the tissue or organ of plants, or for studying of specificity of each development step.
摘要翻译：本发明涉及用于使转基因植物的愈伤组织，各种组织和器官中的GFP表达可视化的方法和使用其的系统。所述方法不需要其他额外的遗传产物，底物或辅因子，并且通过使用由CCD照相机，光源，带通滤波器和数据处理计算机组成的本发明的所述系统，可以非常简单且快速地检测GFP表达，因此它为选择转基因种子，研究植物组织或器官中的基因表达，或研究每个发育步骤的特异性提供了许多优点。

3. 发明公开

KR1020010094812A 형질전환식물체의 생체분석방법 및 그를 이용한 시스템 失效
标题翻译：变形植物及其系统的生物样品分析方法
公开(公告)号：KR1020010094812A
公开(公告)日：2001-11-03
申请号：KR1020000018104
申请日：2000-04-07
申请人： 이춘환 , 주식회사 디비하이텍
发明人： 이춘환 , 김주곤 , 정병철 , 박용주
IPC分类号： G01N33/48
CPC分类号： G01N21/6428
摘要： PURPOSE: A method for analyzing a bio-sample of a transformed plant and a system thereof are provided to simply and speedily detect the generation of GFP without any genes, substrate or auxiliary genes, thereby improving the study or development of gene generation of plant organisms or sorting of transformed seeds. CONSTITUTION: In a method for analyzing a bio-sample of a transformed plant, excited light is generated by using a light source(1), radiating light to a sample of a transformed plant exhibiting GFP(green fluorescent protein) fluorescence by an angle of 45° by passing light in the vicinity of 488nm which exciting the GFP by disposing blue band-pass filter(2) in front of the light source for passing only light of 470-490nm, transmitting light in the vicinity of 500-550nm with a zoom lens(5) by passing light in the vicinity of 509nm with a green band-pass filter(4) when the GFP in the sample becomes fluorescent, taking a photograph of an image by a CCD color video camera(6) attached with the zoom lens, and processing the image by a computer connected to the camera, thereby determining the transformation of the plant sample.
摘要翻译：目的：提供一种分析转化植物的生物样品及其系统的方法，以简单快速地检测GFP的产生，而无需任何基因，底物或辅助基因，从而改善植物生物基因生成的研究或开发或分类转化的种子。构成：在用于分析转化植物的生物样品的方法中，通过使用光源（1）产生激发光，将光照射到呈现GFP（绿色荧光蛋白）荧光的转化植物的样品上，角度为通过在488nm附近通过光，通过在光源前方设置蓝色带通滤光器（2），仅通过470-490nm的光而激发GFP，光在500-550nm附近传输光，变焦透镜（5）通过在绿色带通滤光器（4）中通过使绿色带通滤光器（4）照射到509nm附近，当样品中的GFP变为荧光时，通过附带有CCD的彩色摄像机（6）拍摄图像的照片变焦镜头，并通过与相机连接的计算机处理图像，从而确定植物样本的变换。

4. 发明公开

KR1020010090954A 수박의 종자 발생시 종피특이적으로 유전자의 발현을조절하는 Ｃｖ２０ｏｘ유전자 프로모터 无效
标题翻译： CV20OX基因启动子在番茄发育过程中特异性调节基因表达
公开(公告)号：KR1020010090954A
公开(公告)日：2001-10-22
申请号：KR1020000018483
申请日：2000-04-08
申请人： 주식회사 디비하이텍
发明人： 안진흥 , 강홍규 , 전성훈 , 김준열 , 정기환 , 박용주 , 이상엽
IPC分类号： C12N15/63
CPC分类号： C12N9/0071 , C12N15/8234 , C12N15/8287 , C12N15/8297
摘要： PURPOSE: Cv20ox gene promotor is provided which is separated from Citrullus lanatus seeds on a developmental stage and regulates gene expression spornioderm-specifically. CONSTITUTION: Cv20ox gene promotor is expressed in an integumental tissue of Citrullus lanatus seeds on a developmental stage. Cv20ox gene promotor regulating the gene expression spornioderm-specifically is prepared by following steps of: separating cDNA of Cv20ox gene which codes Gibberellin(GA)20-oxidant Cv20ox; separating Cv20ox gene promotor from total genome DNA using 700bp of 5' upstream region of Cv20ox promotor as PCR cloning kit; and measuring the activity of Cv20ox gene promotor by histochemical detection using expression vector pGA2118. Wherein, The expression vector pGA2118 is prepared by the steps of: cutting vector derivative pGA1230 including GUS reporter gene, with HindIII and PstI; and inserting 0.7kb promotor segment of Cv20ox obtained from a recombinant vector pGA2044.
摘要翻译：目的：提供Cv20ox基因启动子，在发育阶段与柑橘种子分离，特异性调节基因表达。构成：Cv20ox基因启动子在发育阶段在柑橘种子的皮肤组织中表达。通过以下步骤制备Cv20ox基因启动子：通过以下步骤分离编码赤霉素（GA）20氧化剂Cv20ox的Cv20ox基因的cDNA; 使用700bp的Cv20ox启动子的5'上游区域作为PCR克隆试剂盒将Cv20ox基因启动子与总基因组DNA分离; 并用表达载体pGA2118通过组织化学检测来测定Cv20ox基因启动子的活性。其中，通过以下步骤制备表达载体pGA2118：用HindIII和PstI切割包含GUS报告基因的载体衍生物pGA1230; 并插入从重组载体pGA2044获得的0.7kb的Cv20ox启动子片段。

5. 发明公开

KR1020040037611A 브로콜리의 체세포배 배양 방법 및 그를 이용한 브로콜리식물체싹의 대량 생산 방법 失效
标题翻译：用于培养BROCCOLI的人造胚的方法和使用该方法生产BROCCOLI SPROUTS的方法
公开(公告)号：KR1020040037611A
公开(公告)日：2004-05-07
申请号：KR1020020066165
申请日：2002-10-29
申请人： 주식회사 디비하이텍
发明人： 박용주 , 윤은정 , 김형태 , 정기환 , 우은택
IPC分类号： A01H4/00
CPC分类号： A01H4/005 , A01H4/001 , C12N5/0025 , C12N2501/30
摘要： PURPOSE: A method for culturing a somatic embryo of broccoli and a method for mass-producing broccoli sprouts using the same are provided, thereby from the somatic embryo of broccoli rapidly and easily mass-producing broccoli sprouts which are used in anticancer foods or medicines. CONSTITUTION: A method for culturing a somatic embryo of broccoli comprises the steps of: (1) culturing broccoli tissue which is selected from seeds, anther, root, stem and leaf of broccoli and is capable of forming an embryo in a medium to produce the broccoli embryo; (2) cutting the broccoli embryo and culturing it on 2,4-D containing medium to form callus of broccoli; (3) forming embryo-developed cells from the callus; and (4) subculturing the embryo-developed cells in a hormone free medium. A method for mass-producing broccoli sprouts comprises the steps of: (1) selecting embryo-developed cells and culturing them; (2) proliferating the embryo-developed cells and isolating cell mass; and (3) mass-culturing the cell mass.
摘要翻译：目的：提供用于培养西兰花体细胞胚的方法和使用该方法大量生产西兰花芽的方法，从而从西兰花的体细胞胚迅速且容易地批量生产用于抗癌食品或药物的西兰花芽。构成：用于培养西兰花体细胞胚的方法包括以下步骤：（1）培养选自西兰花的种子，花药，根，茎和叶的花椰菜组织，并且能够在培养基中形成胚胎以产生西兰花胚胎; （2）切割西兰花胚，并在含2,4-D培养基上培养以形成西兰花的愈伤组织; （3）从愈伤组织形成胚胎发育的细胞; 和（4）在无激素培养基中将胚胎发育的细胞传代培养。大量生产西兰花芽苗的方法包括以下步骤：（1）选择胚胎发育的细胞并培养它们; （2）增殖胚胎发育的细胞并分离细胞团; 和（3）大量培养细胞团。

6. 发明公开

KR1020020093028A 수박의 종자 발생시 종피 특이적으로 유전자의 발현을조절하는 Ｃｖ20ｏｘＰ 유전자 프로모터 및 이를 이용한씨 없는 식물체의 제조방법 无效
标题翻译：促进剂CV20OXP调节GIBBERELLIN 20氧化酶基因在开发水稻种子中的综合特异性表达和使用促进剂产生无籽水果的方法
公开(公告)号：KR1020020093028A
公开(公告)日：2002-12-12
申请号：KR1020027013435
申请日：2000-10-09
申请人： 주식회사 디비하이텍
发明人： 안진흥 , 강홍규 , 전성훈 , 김준열 , 정기환 , 박용주 , 이상엽
IPC分类号： C12N15/53
CPC分类号： C12N9/0071 , C12N15/8234 , C12N15/8287 , C12N15/8297
摘要： PURPOSE: Provided are a testa specific gene, which is isolated from the developing seed of Citrullus lanatus, a promoter and a method for generating seedless fruits by using the promoter of the said testa specific gene. CONSTITUTION: The Cv20ox gene, gibberellin 20-oxidase(GA 20-oxidase) gene showing the tissue-specific expression pattern, is strongly expressed in the integumental tissue at seed development of watermelon and is weakly expressed in the inner tissue of seed. The promoter regulates the integument-specific expression of the gene and the method for generating seedless fruits is characterized by introducing plant growth regulation gene ligated to the promoter and regulating the overexpression of plant growth regulation composition in the testa.
摘要翻译：目的：提供一种睾丸特异性基因，其通过使用所述睾丸特异性基因的启动子从柑橘柑橘的发育种子，启动子和产生无核果实的方法中分离。构成：显示组织特异性表达模式的赤霉素20-氧化酶（GA 20-氧化酶）基因的Cv20ox基因在西瓜种子发育中在皮肤组织中强烈表达，并且在种子的内部组织中弱表达。启动子调节基因的特异性表达，产生无核果实的方法的特征在于引入连接到启动子的植物生长调节基因并调节试验中植物生长调节组成的过表达。

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