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    • 37. 发明申请
    • AUTOMATIC CULTURE DEVICE
    • 自动文化装置
    • US20130143307A1
    • 2013-06-06
    • US13816069
    • 2010-08-12
    • Takayuki NozakiToyoshige KobayashiShizu MatsuokaRyota Nakajima
    • Takayuki NozakiToyoshige KobayashiShizu MatsuokaRyota Nakajima
    • C12M1/36
    • C12M1/36C12M23/14C12M23/42C12M27/10C12M27/20C12M29/10C12M29/20C12M41/00
    • In order to uniformly sow cells on the culture surface of a cartridge-type closed culture vessel and remove bubbles contaminating in liquid culture medium in the course of automatic culture, an automatic culture equipment is provided with a flow-controlling mechanism section for solving the non-uniformity in cell distribution by, after filling up a culture space in the cartridge-type closed culture vessel, said cartridge-type closed culture vessel being in an upright position, with cell suspension, turning the cartridge-type closed culture vessel into a horizontal position, and then repeatedly supplying the cell suspension and sucking the same multiple times to thereby create a mixing flow in the cell suspension. In this process, the liquid in channels is efficiently sent by applying a reduced pressure and an elevated pressure respectively to a channel on one side and a channel on the opposite side, using two syringes and check valves connected to the channels, to thereby load forces to the liquid from both sides. The liquid can be sent without any cell loss by conducting the same operations of the flow-controlling mechanism section in a tank and in a cell bag.
    • 为了在筒式封闭培养容器的培养表面上均匀地播种细胞,并且在自动培养过程中去除在液体培养基中污染的气泡,自动培养装置设有流动控制机构部分, - 细胞分布均匀性,在填充盒式封闭培养容器中的培养空间后,所述盒式封闭培养容器处于直立位置,具有细胞悬浮液,将盒式封闭培养容器转换成水平 位置,然后反复供给细胞悬浮液并多次吸吮,从而在细胞悬液中产生混合流。 在这个过程中,通过使用两个注射器和连接到通道的止回阀,将减压和升高的压力分别施加到一侧的通道和相对侧上的通道上来有效地发送通道中的液体,从而加载力 来自双方的液体。 通过进行槽内和流通池中的流动控制机构部分的相同操作,可以不发生任何细胞损失而送出液体。