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    • 37. 发明授权
    • Method for increasing the content of docosahexaenoic acid in fat-containing materials or in fats and oils
    • 增加含脂肪物质或脂肪和油中二十二碳六烯酸含量的方法
    • US08349595B2
    • 2013-01-08
    • US12524647
    • 2008-01-25
    • Masahiro HayashiSanae NakajimaNaoki NaganoYosuke Taoka
    • Masahiro HayashiSanae NakajimaNaoki NaganoYosuke Taoka
    • C12P7/64C12N1/12C07C57/03
    • C12P7/6427C11C3/00C12N1/14C12P7/6472
    • [PROBLEMS] To provide a fat-and-oil in which the content of docosahexaenoic acid is increased.[MEANS FOR SOLVING PROBLEMS] A process for producing highly unsaturated fatty acids comprising culturing a stramenopile capable of producing highly unsaturated fatty acids in a culture medium containing an inhibitor for fatty acid desaturases; fats-and-oils in which the content of highly unsaturated fatty acids, particularly docosahexaenoic acid produced by the relevant method, is increased. A method for enhancing the productivity of highly unsaturated fatty acids in stramenopiles, comprising culturing a stramenopile in a culture medium containing an inhibitor for fatty acid desaturases; stramenopiles having the enhanced productivity of highly unsaturated fatty acids, generated by the relevant method. Particularly, the process for producing highly unsaturated fatty acids, the method for enhancing the productivity of highly unsaturated fatty acids, and the stramenopile having the enhanced productivity of highly unsaturated fatty acids, wherein the stramenopile is a microorganism classified into Labyrinthulea.
    • [问题]提供其中二十二碳六烯酸含量增加的脂肪和油。 解决问题的手段一种高不饱和脂肪酸的制造方法,其特征在于,在含有脂肪酸去饱和酶抑制剂的培养基中培养能够生产高度不饱和脂肪酸的前列腺素; 其中通过相关方法生产的高度不饱和脂肪酸,特别是二十二碳六烯酸的含量增加的脂肪和油。 一种增强层状高分子量不饱和脂肪酸的生产率的方法,包括在含有脂肪酸去饱和酶抑制剂的培养基中培养前列腺素; 具有通过相关方法生成的高度不饱和脂肪酸的生产率提高的前列腺素。 特别是生产高不饱和脂肪酸的方法,提高高不饱和脂肪酸的生产率的方法以及具有提高的高度不饱和脂肪酸的生产率的前列腺素,其中所述地釉菌是分类为迷迭虫的微生物。
    • 38. 发明申请
    • METHOD FOR DETERMINING FATTY ACID SYNTHESIS PATHWAY OF MICROORGANISM, AND PCR PRIMER SET FOR USE IN THE METHOD
    • 用于确定微生物的脂肪酸合成途径的方法,以及用于该方法的PCR引物组
    • US20120100550A1
    • 2012-04-26
    • US13254794
    • 2010-03-04
    • Naoki NaganoMasahiro HayashiKeishi SakaguchiMakoto ItoYosuke TaokaYuji Okita
    • Naoki NaganoMasahiro HayashiKeishi SakaguchiMakoto ItoYosuke TaokaYuji Okita
    • C12Q1/68
    • C12Q1/689
    • Disclosed is a method for determining the fatty acid synthesis pathway of a microorganism. Specifically disclosed is a method for determining the fatty acid synthesis pathway of a microorganism, which is characterized by producing degenerate primers for a fatty acid synthesis-related enzyme gene and determining the presence or absence of the fatty acid synthesis-related gene in the genome gene extracted from the microorganism using the degenerate primers. The sequences for degenerate primers for C20-elongase gene, which is a fatty acid synthesis-related enzyme gene, are ATHGARTWYTKBRTITTYGTICA (SEQ ID NO:1) and TARTRISWRTACATIADIAMRTG (SEQ ID NO:2), and the sequences for degenerate primers for ?4-desaturase gene are GGNCAYCAYCMITAYACNAA (SEQ ID NO:3) and TCDATYTGRTGIBWIARNCC (SEQ ID NO:4). The microorganism is one belonging to the class Labyrinthulea. Also specifically disclosed is a PCR primer set for use in the determination method. The method is useful as a method for screening a microorganism capable of synthesizing a fatty acid.
    • 公开了一种测定微生物的脂肪酸合成途径的方法。 具体公开的是测定微生物的脂肪酸合成途径的方法,其特征在于制备脂肪酸合成相关酶基因的简并引物,并测定基因组基因中脂肪酸合成相关基因的存在或不存在 使用简并引物从微生物中提取。 作为脂肪酸合成相关酶基因的C20延伸酶基因的简并引物的序列是ATHGARTWYTKBRTITTYGTICA(SEQ ID NO:1)和TARTRISWRTACATIADIAMRTG(SEQ ID NO:2),并且用于Δ4的简并引物的序列 - 脱氢酶基因是GGNCAYCAYCMITAYACNAA(SEQ ID NO:3)和TCDATYTGRTGIBWIARNCC(SEQ ID NO:4)。 该微生物属于Labyrinthulea类。 还具体公开了用于确定方法的PCR引物组。 该方法可用作筛选能够合成脂肪酸的微生物的方法。
    • 40. 发明申请
    • CABLE PROTECTION DEVICE
    • 电缆保护装置
    • US20100213316A1
    • 2010-08-26
    • US12678814
    • 2008-02-25
    • Masahiro Hayashi
    • Masahiro Hayashi
    • B64D1/22
    • B64D1/22B64C25/52B64C27/04B64C2025/325B64D47/08
    • PROBLEM TO BE SOLVED: To prevent a cable from being damaged by rubbing against a helicopter.SOLUTION: A protection frame 2 is installed on a side surface of a machine body below a winch 11 equipped with a helicopter 10. A supporting groove 213 of a circular cross section long in a horizontal direction is formed on the protection frame 2, and a slide-contact body 3 of a cylindrical shape is rotatably fitted in the support groove 213. A part of the peripheral surface of the slide-contact body 3 fitted from an opening part of the supporting groove 213 is exposed, and the cable 12 hung down from the winch 11 is brought into contact with the exposed portion. The slide contact body 3 is divided into two or more parts, and it is formed by continuously aligning the divided slide-contact bodies 30 of the cylindrical shape. Both ends of the divided slide-contact body 30 are formed to recession-like or projection-like taper shapes 31, 32, and they are aligned by fitting the recession and the projection of the tapers 31, 32 of the ends of the adjacent divided slide-contact bodies 30 to each other.
    • 要解决的问题:防止电缆与直升机摩擦而损坏。 解决方案:保护框架2安装在装备有直升机10的绞盘11下面的机体的侧表面上。在保护框架2上形成有沿水平方向长的圆形横截面的支撑槽213, 圆筒形的滑动接触体3可旋转地装配在支撑槽213中。滑动接触体3的从支撑槽213的开口部分装配的一部分周面露出,电缆12被悬挂 从绞盘11与露出部接触。 滑动接触体3被分成两部分,并且通过使圆柱形的分割的滑动接触体30连续对准而形成。 分开的滑动接触体30的两端形成为凹陷状或突起状的锥形形状31,32,并且通过将相邻的分割的滑动接触体30的端部的锥形部31,32的凹部和突起嵌合 滑动接触体30彼此连接。