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31. 发明授权

US07901633B2 Quantitative cell dispensing apparatus using liquid drop manipulation 有权
标题翻译：使用液滴操作的定量细胞分配装置
公开(公告)号：US07901633B2
公开(公告)日：2011-03-08
申请号：US11491825
申请日：2006-07-24
申请人： Nam Huh , Jeong-gun Lee
发明人： Nam Huh , Jeong-gun Lee
IPC分类号： B01L3/02 , G01N1/10
CPC分类号： B01L3/0241 , B01L2200/0647 , B01L2200/0673 , B01L2200/143 , B01L2300/0819 , B01L2300/089 , B01L2400/027 , B01L2400/0427 , G01N2035/00237 , Y10T436/2575
摘要： Provided is a quantitative cell dispensing apparatus using liquid droplet manipulation, for quantitatively dispensing cells from hydrophilic suspension containing a plurality of cells. The quantitative cell dispensing apparatus includes hydrophilic suspension, a liquid droplet manipulation unit, a dispensing unit, a sensing unit, and a control unit. The hydrophilic suspension includes a plurality of cells and electrolyte, and the liquid droplet manipulation unit. The liquid droplet manipulation unit includes an inlet and a liquid droplet outlet portion. The dispensing unit discharges a liquid droplet received from the liquid droplet outlet portion to a target portion. The sensing unit optically senses liquid droplets distributed on a panel of the liquid droplet manipulation unit. The control unit analyses the number of cells contained in each liquid droplet using a single sensed by the sensing unit, and controls the liquid droplet manipulation unit to transfer a liquid droplet containing a predetermined number of cells to the liquid droplet outlet portion.
摘要翻译：提供了一种使用液滴操作的定量细胞分配装置，用于从含有多个细胞的亲水性悬浮液定量分配细胞。定量细胞分配装置包括亲水性悬浮液，液滴操作单元，分配单元，感测单元和控制单元。亲水性悬浮液包括多个细胞和电解质，以及液滴操作单元。液滴操作单元包括入口和液滴出口部分。分配单元将从液滴出口部分接收的液滴排放到目标部分。感测单元光学地感测分布在液滴操作单元的面板上的液滴。控制单元使用感测单元检测到的单个分析每个液滴中包含的细胞的数量，并且控制液滴操作单元将含有预定数量的细胞的液滴转移到液滴出口部分。

32. 发明授权

US07819138B2 Microfluidic device using centrifugal force and pump to control fluid movement, microfluidic system comprising the same and method of manufacturing the microfluidic device 有权
标题翻译：使用离心力和泵来控制流体运动的微流体装置，包括其的微流体系统和制造微流体装置的方法
公开(公告)号：US07819138B2
公开(公告)日：2010-10-26
申请号：US11869835
申请日：2007-10-10
申请人： Hun-joo Lee , Nam Huh , Soo-suk Lee , Sung-ouk Jung , Soo-hyung Choi
发明人： Hun-joo Lee , Nam Huh , Soo-suk Lee , Sung-ouk Jung , Soo-hyung Choi
IPC分类号： F15C1/04
CPC分类号： G05D7/0694 , Y10T29/494 , Y10T137/0753 , Y10T137/2191 , Y10T137/86187
摘要： The microfluidic device includes a rotatable platform, a plurality of connection ports disposed at a portion of the platform proximate to a shaft connection hole, the plurality of connection ports capable of being connected to an external connector for selectively injecting and discharging fluid and being closed by the connector, a trap chamber disposed at a portion of the platform further away from the shaft connection hole than the plurality of connection ports, the trap chamber including an inlet connected with at least one connection port of the plurality of connection ports, an outlet connected with another connection port of the plurality of connection ports and structures which enlarge a contact area with the fluid and a temporary storage including an inlet connected with the outlet of the trap chamber and an outlet connected with another connection port of the plurality of connection ports.
摘要翻译：所述微流体装置包括可旋转平台，多个连接端口，设置在所述平台的靠近轴连接孔的部分处，所述多个连接端口能够连接到外部连接器，用于选择性地注入和排出流体并且由所述连接器，设置在所述平台的远离所述轴连接孔的所述平台部分处的所述收集室，所述收集室包括与所述多个连接端口中的至少一个连接端口连接的入口，所述入口连接到所述入口多个连接端口中的另一个连接端口和扩大与流体的接触面积的结构以及包括与捕集室的出口连接的入口的临时存储器和与多个连接端口中的另一连接端口连接的出口。

33. 发明授权

US07687259B2 Method for noncovalently immobilizing a biomolecule on a solid substrate and microarray produced according to the method 失效
标题翻译：用于非固定地将生物分子固定在固体基质上的方法和根据该方法制备的微阵列
公开(公告)号：US07687259B2
公开(公告)日：2010-03-30
申请号：US12145921
申请日：2008-06-25
申请人： Nam Huh , Jong-myeon Park
发明人： Nam Huh , Jong-myeon Park
IPC分类号： C12M1/34 , C12Q1/68 , G01N33/53 , C07K1/10
CPC分类号： C12Q1/6837 , B01J2219/00387 , B01J2219/00529 , B01J2219/00596 , B01J2219/00608 , B01J2219/00612 , B01J2219/0063 , B01J2219/00637 , B01J2219/00659 , B01J2219/00677 , C40B60/14 , G01N33/54393
摘要： Provided is a method for noncovalently immobilizing a biomolecule on a solid substrate, including: providing a solid substrate having a first functional group attached thereto, the first functional group having a hydrogen bond donating ability; and reacting a mixture of a compound having a hydrogen bond accepting ability and a biomolecule functionalized with a second functional group, with the surface of the substrate, the second functional group having a hydrogen bond donating ability, in order to noncovalently immobilize the biomolecule on the substrate.
摘要翻译：本发明提供一种在固体基质上非共价固定生物分子的方法，包括：提供具有第一官能团的固体基质，具有氢键赋予能力的第一官能团; 并使具有氢键接受能力的化合物和用第二官能团官能化的生物分子的混合物与底物的表面，第二官能团具有氢键供体能力反应，以将生物分子非常固定地固定在基质。

34. 发明授权

US07648838B2 Cell lysis method by immobilized metal-ligand complex 失效
标题翻译：固定化金属配体复合物的细胞裂解法
公开(公告)号：US07648838B2
公开(公告)日：2010-01-19
申请号：US11267005
申请日：2005-11-04
申请人： Chang-eun Yoo , Joon-ho Kim , Kyu-youn Hwang , Hun-joo Lee , Hee-kyun Lim , Soo-min Ma , Nam Huh , Soo-suk Lee
发明人： Chang-eun Yoo , Joon-ho Kim , Kyu-youn Hwang , Hun-joo Lee , Hee-kyun Lim , Soo-min Ma , Nam Huh , Soo-suk Lee
IPC分类号： G01N33/48 , G01N33/20 , G01N1/00
CPC分类号： C12Q1/6806 , Y10T436/25 , C12Q2523/308
摘要： Provided is a lysis method for cells or viruses, including: immobilizing a metal-ligand complex on a solid support; and mixing the complex immobilized on the support with a cell or virus solution. According to the lysis method, by immobilizing a chemical on a solid support to perform cell lysis, the dilution problem according to the addition of a cell lysis solution can be resolved and a separate process of removing the chemical is not required so as to reduce the steps upon LOC implementation. In addition, since a variety of solid supports, such as chips, beads, nanoparticles etc. can be used, cell lysis apparatuses of various forms can be fabricated.
摘要翻译：提供了一种细胞或病毒的裂解方法，包括：将固定支持物上的金属 - 配体复合物固定; 并将固定在载体上的复合物与细胞或病毒溶液混合。根据裂解方法，通过将化学物质固定在固体支持体上以进行细胞裂解，可以解决根据细胞溶解溶液的添加的稀释问题，并且不需要单独的除去化学品的方法，以减少 LOC实施步骤。此外，由于可以使用各种固体支持物，例如芯片，珠粒，纳米粒子等，因此可以制造各种形式的细胞裂解装置。

35. 发明申请

US20080226504A1 PUMP UNIT AND CENTRIFUGAL MICROFLUIDIC SYSTEM HAVING THE SAME 审中-公开
标题翻译：泵单元和离心微流体系统
公开(公告)号：US20080226504A1
公开(公告)日：2008-09-18
申请号：US11861324
申请日：2007-09-26
申请人： Jong-myeon PARK , Nam Huh , Jeong-gun Lee , Kyu-youn Hwang
发明人： Jong-myeon PARK , Nam Huh , Jeong-gun Lee , Kyu-youn Hwang
IPC分类号： B04B5/10
CPC分类号： B01L3/50273 , B01L3/502738 , B01L2300/0803 , B01L2400/0442 , B01L2400/0487 , B01L2400/0677 , F04B19/006 , F04B19/24 , F04F1/06
摘要： Provided are a pump unit and a centrifugal microfluidic system including the pump unit. The pump unit includes a gas generating agent. The gas generating agent includes a plurality of heating particles generating heat by absorbing energy and one of a sublimation material, azide, and azo compound mixed with the heating particles. When energy is supplied to the gas generating agent, the gas generating agent generates gas due to heat emitted from the heating particles to increase an air pressure around the gas generation agent so that the fluid is moved by the increased air pressure.
摘要翻译：提供了包括泵单元的泵单元和离心微流体系统。泵单元包括气体发生剂。气体发生剂包括通过吸收能量产生热的多个加热颗粒和与加热颗粒混合的升华材料，叠氮化物和偶氮化合物之一。当向气体发生剂供应能量时，气体发生剂由加热颗粒发出的热量产生气体，从而增加气体发生剂周围的空气压力，使得流体通过增加空气压力而移动。

36. 发明申请

US20060255267A1 Device for printing biomolecules on substrate using electrohydrodynamic effect 审中-公开
标题翻译：使用电动力学效应在基板上印刷生物分子的装置
公开(公告)号：US20060255267A1
公开(公告)日：2006-11-16
申请号：US11418602
申请日：2006-05-05
申请人： Jeong-gun Lee , Jeong-woo Choi , Nam Huh , Hye-jung Cho
发明人： Jeong-gun Lee , Jeong-woo Choi , Nam Huh , Hye-jung Cho
IPC分类号： G21K7/00
CPC分类号： B01L3/0244 , B01J2219/0036 , B01J2219/00378 , B01J2219/00659 , B01J2219/00722 , B01J2219/00725 , B01J2219/00729 , B01L3/0241 , B01L2300/0819
摘要： Provided is a device for printing a biomolecule solution onto a substrate using an electrohydrodynamic (EHD) effect. The device can deposit drops of the biomolecule solution with small size and volume and print more of the biomolecule solution onto a substrate, thereby enabling the manufacture of a high density biochip. The device includes a first electric field forming electrode that is shaped like a needle, is made of a conductive material, is disposed vertically, and includes an accommodating area in which the biomolecule solution is accommodated and an outlet formed on a bottom end of the accommodating area through which the biomolecule solution is discharged; a substrate disposed below the first electric field forming electrode, and including a target surface onto which the biomolecule solution discharged from the outlet of the first electric field forming electrode is deposited; a second electric field forming electrode made of a conductive material and disposed below the first electric field forming electrode; and a voltage applying device which is electrically connected to the first and second electric field forming electrodes to apply a voltage between the first and second electric field forming electrodes so that an electric field is generated around the biomolecule solution suspended in the outlet, wherein, due to the interaction between the electric field and a difference between dielectric constants of the biomolecule solution having a free surface and the surrounding atmosphere, the electric force acts inward on the biomolecule solution, thereby causing a predetermined amount of the biomolecule solution to drop onto the target surface of the substrate.
摘要翻译：提供了使用电动力学（EHD）效应将生物分子溶液印刷到基底上的装置。该装置可以沉积小尺寸和体积的生物分子溶液，并将更多的生物分子溶液印刷到基底上，从而能够制造高密度生物芯片。该装置包括成形为针状的第一电场形成电极，由导电材料制成，垂直设置，并且包括其中容纳有生物分子溶液的容纳区域和形成在容纳物的底端的出口生物分子溶液排出的区域; 设置在所述第一电场形成电极下方的基板，并且包括从所述第一电场形成用电极的出口排出的生物分子溶液沉积在其上的目标表面; 由导电材料制成并设置在第一电场形成电极下方的第二电场形成电极; 以及电压施加装置，其电连接到第一和第二电场形成电极，以在第一和第二电场形成电极之间施加电压，使得在悬浮在出口中的生物分子溶液周围产生电场，其中，由于对于电场与具有自由表面和周围大气的生物分子溶液的介电常数之间的差异的相互作用，电力向内作用于生物分子溶液，从而使预定量的生物分子溶液落到目标上基板的表面。

37. 发明申请

US20060154360A1 Hybridization system using the control of pump and valves in closed system 失效
标题翻译：混合系统使用封闭系统中的泵和阀的控制
公开(公告)号：US20060154360A1
公开(公告)日：2006-07-13
申请号：US11295843
申请日：2005-12-06
申请人： Soo-suk Lee , Sung-ouk Jung , Chang-eun Yoo , Hun-joo Lee , Christopher Ko , Nam Huh
发明人： Soo-suk Lee , Sung-ouk Jung , Chang-eun Yoo , Hun-joo Lee , Christopher Ko , Nam Huh
IPC分类号： C12M1/34
CPC分类号： B01L7/00 , B01F13/0059 , B01F13/0222 , B01L3/5027 , B01L2200/0689 , B01L2300/0636 , B01L2300/14 , B01L2300/1822 , B01L2400/0487 , G01N2035/00544
摘要： Provided is a hybridization system for hybridizing a biochip including: a chamber device including at least a hybridization chamber including a support for a biochip and a first cover having a sample inlet; an agitation device including: two air channels connected to ends of the hybridization chamber; two valves disposed in the air channels; an integrated air channel to which the two air channels are connected; and an air pump disposed in the integrated air channel; and a washing and drying device including: a flow channel connected to one of the two air channels through a branched valve; a flow pump disposed in the flow channel; and a buffer inlet disposed opposite the flow channel.
摘要翻译：提供了一种用于杂交生物芯片的杂交系统，包括：至少包括一个包括用于生物芯片的支撑物的杂交室的腔室装置和具有样品入口的第一盖子; 一种搅拌装置，包括：两个连接到杂交室端部的空气通道; 设置在空气通道中的两个阀; 两个空气通道连接的集成空气通道; 以及设置在所述集成空气通道中的空气泵; 以及洗涤干燥装置，包括：流路，其通过分支阀连接到所述两个空气通道中的一个; 设置在流路中的流量泵; 以及与流动通道相对设置的缓冲器入口。

38. 发明申请

US20060147960A1 Hybridization chamber agitation device using pump and valves 失效
公开(公告)号：US20060147960A1
公开(公告)日：2006-07-06
申请号：US11295844
申请日：2005-12-06
申请人： Sung-ouk Jung , Nam Huh , Soo-suk Lee , Chang-eun Yoo , Hun-joo Lee
发明人： Sung-ouk Jung , Nam Huh , Soo-suk Lee , Chang-eun Yoo , Hun-joo Lee
IPC分类号： C12Q1/68 , C12M1/34 , B01F13/02
CPC分类号： B01L3/502738 , B01F13/0059 , B01F13/0222 , B01L3/50273 , B01L7/52 , B01L2300/0636 , B01L2300/0822 , B01L2300/0877
摘要： Provided is an agitation device used to agitate a solution in a hybridization chamber, the agitation device including: the hybridization chamber; first and second air channels connected to ends of the hybridization chamber; a first valve disposed in the first air channel; a second valve disposed in the second air channel; an integrated air channel connecting the first and second air channels; and a pump disposed in the integrated air channel. The agitation device is suitable for effective diffusion of a sample when performing hybridization using a DNA chip. Therefore, a probe can be effectively hybridized with a target material.

39. 发明申请

US20050244871A1 Polynucleotide probe having enhanced binding specificity, microarray having the probe immobilized thereon, and method of designing the probe 审中-公开
标题翻译：具有增强的结合特异性的多核苷酸探针，其上固定有探针的微阵列，以及设计探针的方法
公开(公告)号：US20050244871A1
公开(公告)日：2005-11-03
申请号：US11110281
申请日：2005-04-20
申请人： Ji-young Oh , Nam Huh
发明人： Ji-young Oh , Nam Huh
IPC分类号： G01N33/53 , C12M1/00 , C12N15/09 , G01N37/00 , C12Q1/68 , G06F19/00 , G01N33/48 , G01N33/50
CPC分类号： C12Q1/6832 , C12Q2527/107
摘要： An oligonucleotide probe, a microarray having the probe immobilized thereon, and a method of designing the probe are provided. In the oligonucleotide probe, a difference between a hybridization temperature (Tm) of an oligonucleotide sequence including the first nucleotide in the direction of 5′ end from a nucleotide corresponding to a target site through 5′ end nucleotide and an oligonucleotide sequence complementary thereto and a hybridization temperature (Tm) of an oligonucleotide sequence including the first nucleotide in the direction of 3′ end from the nucleotide corresponding to the target site through 3′ end nucleotide and an oligonucleotide sequence complementary thereto is not greater than 5° C.
摘要翻译：提供寡核苷酸探针，其上固定有探针的微阵列和设计探针的方法。在寡核苷酸探针中，从对应于目标部位至5'末端核苷酸的核苷酸的核苷酸对应的5'末端方向的第一核苷酸的寡核苷酸序列与其互补的寡核苷酸序列的杂交温度（Tm）与通过3'末端核苷酸对应于目标部位的核苷酸和与其互补的寡核苷酸序列，在3'末端方向上包含第1个核苷酸的寡核苷酸序列的杂交温度（Tm）不大于5℃。

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