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    • 32. 发明申请
    • METHOD FOR PRODUCING A LARGE REGION DUPLICATION OF ASPERGILLUS CHROMOSOME
    • 用于生产青US色素的大区域重叠的方法
    • US20130171319A1
    • 2013-07-04
    • US13704029
    • 2011-05-25
    • Tadashi Takahashi
    • Tadashi Takahashi
    • C12N15/80
    • C12N15/80A23L27/50
    • Disclosed is a technique which is for duplicating across a wide region any large region on a chromosome of a fungus belonging to Aspergillus strain, and which enables the stable and systematic acquisition of a previously un-acquirable fungus belonging to the Aspergillus strain having novel traits. Disclosed is a transformant wherein a transformation marker gene sequence is arranged on the outside of terminals (5′, 3′) of a region so as to sandwich an arbitrary region of a chromosome, and chromosome duplication has taken place by means of homologous recombination after double strand breakage generated in a homologous region inside the gene. Also disclosed is a method for duplicating an arbitrary region of a chromosome of a fungus belonging to Aspergillus strain by culturing said transformant.
    • 公开了一种技术,用于在广泛区域上复制属于曲霉菌属菌株的真菌染色体上的任何大区域,并且其能够稳定和系统地获取具有新特征的属于曲霉菌属的先前不可获得的真菌。 披露了转化标记基因序列设置在区域的末端(5',3')的外侧,以夹持染色体的任意区域,并且通过同源重组后发生染色体复制的转化体 在基因内的同源区域产生双链断裂。 还公开了通过培养所述转化体来复制属于曲霉属菌株的真菌染色体的任意区域的方法。
    • 35. 发明申请
    • Method for the deletion of a large chromosomal region
    • 删除大染色体区域的方法
    • US20090035863A1
    • 2009-02-05
    • US12081999
    • 2008-04-24
    • Tadashi TakahashiYasuji Koyama
    • Tadashi TakahashiYasuji Koyama
    • C12N15/80C12N1/15
    • C12N15/80
    • In order to efficiently delete a large chromosomal region with a length of from several tens to hundreds kb such as a cluster of biosynthesis genes encoding a toxic substance such as Aflatoxin, the present invention provides a method for the deletion of a large chromosomal region comprising transforming a transformant having an increased frequency of homologous recombination due to suppression of a Ku gene, which is a mitosporic filamentous fungus belonging to Trichocomaceae with a vector for the deletion of a chromosomal region, which comprises a pair of homologous region arms having a nucleotide sequence that is homologous with a fragment at either end of said chromosomal region, and deleting the chromosomal region by means of homologous recombination between the resulting transformant and said vector.
    • 为了有效地删除长度为几十到几百kb的大染色体区域,例如编码毒性物质如黄曲霉毒素的生物合成基因簇,本发明提供了一种删除大染色体区域的方法,包括转化 由于抑制Ku基因而具有增加的同源重组频率的转化体,该Ku基因是具有用于缺失染色体区域的载体的属于三齿科的丝裂霉丝状真菌,其包含一对具有核苷酸序列的同源区域臂, 与所述染色体区域的任一端的片段同源,并通过所得转化体和所述载体之间的同源重组来删除染色体区域。
    • 38. 发明授权
    • Electronic endoscope system
    • 电子内窥镜系统
    • US06690409B1
    • 2004-02-10
    • US09396374
    • 1999-09-15
    • Tadashi Takahashi
    • Tadashi Takahashi
    • H04N718
    • H04N7/183
    • In an electronic endoscope system, a flexible scope has a solid image sensor provided at a distal end thereof, to generate image-pixel signals. A proximal end of the scope is detachably connected to a video-signal processing unit, which produces a video signal based on the image-pixel signals. A monitor reproduces an image in accordance with the video signal. A displaying-size changer changes a size of a displaying-area of the image on a screen of the monitor to another size and vice versa. A pointer-image generator generates a pointer image on the monitor screen. A pointer-image controller controls a shifting of the pointer image on the monitor screen, and includes a pointer-image-position converter that converts a position of the pointer image on the monitor screen in accordance with the size-change of the displaying-area of the image on the monitor screen, such that two positions, indicated by the pointer image on the two-sized displaying-areas, correspond to each other.
    • 在电子内窥镜系统中,柔性镜具有设置在其远端的实心图像传感器,以产生图像像素信号。 示波器的近端可拆卸地连接到视频信号处理单元,该视频信号处理单元基于图像像素信号产生视频信号。 监视器根据视频信号再现图像。 显示尺寸改变器将监视器的屏幕上的图像的显示区域的大小改变为另一尺寸,反之亦然。 指针图像生成器在监视器屏幕上生成指针图像。 指针图像控制器控制指示器图像在监视器屏幕上的偏移,并且包括指针图像位置转换器,其根据显示区域的尺寸变化而在监视器屏幕上转换指针图像的位置 ,使得由两个尺寸的显示区域上的指针图像指示的两个位置彼此对应。
    • 40. 发明授权
    • Device for controlling an amount of light of a lighting unit for an endoscope
    • 用于控制用于内窥镜的照明单元的光量的装置
    • US06524236B2
    • 2003-02-25
    • US09867433
    • 2001-05-31
    • Ryoji HondaMakoto TakadaTadashi Takahashi
    • Ryoji HondaMakoto TakadaTadashi Takahashi
    • A61B1045
    • G01J1/08A61B1/045A61B1/0638A61B1/0669A61B1/07G01J1/04G01J1/0437G01J1/044G01J1/0492G02B23/2461G02B26/02H04N5/2354H04N2005/2255
    • A device for controlling an amount of light of a lighting unit for use in an endoscope that is used to view an image of an object. A light shielding system shields light generated by a light source and transmitted to the endoscope and a stepping motor drives the light shielding system for a plurality of predetermined time intervals. A system detects a brightness of the image during each of the plurality of predetermined time intervals and an input system inputs one of a plurality of desired brightnesses of the image. A generating system generates a predetermined number of pulses during each of the plurality of predetermined time intervals, the predetermined number of pulses being transmitted to the stepping motor and a system determines an angular position of the light shielding system. A setting system sets one of a plurality of allowed brightness ranges of the image in response to the determined angular position of the light shielding system and a system determines whether the detected brightness is within the set one of the plurality of allowed brightness ranges.
    • 一种用于控制用于观察物体的图像的内窥镜中使用的照明单元的光量的装置。 遮光系统屏蔽由光源产生的光并传输到内窥镜,并且步进电机以多个预定时间间隔驱动遮光系统。 系统在多个预定时间间隔的每一个期间检测图像的亮度,并且输入系统输入图像的多个期望亮度中的一个。 生成系统在多个预定时间间隔的每一个期间产生预定数量的脉冲,预定数量的脉冲被发送到步进电机,并且系统确定遮光系统的角度位置。 设置系统响应于所确定的遮光系统的角度位置来设置图像的多个允许的亮度范围中的一个,并且系统确定检测到的亮度是否在多个允许的亮度范围的设定的一个之内。