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31. 发明授权

US06228997B1 Transesterification of insoluble polysaccharides 失效
标题翻译：不溶性多糖的酯交换
公开(公告)号：US06228997B1
公开(公告)日：2001-05-08
申请号：US09192209
申请日：1998-07-10
申请人： Joseph A. Akkara , David L. Kaplan , Ferdinando F. Bruno , Jonathan S. Dordick
发明人： Joseph A. Akkara , David L. Kaplan , Ferdinando F. Bruno , Jonathan S. Dordick
IPC分类号： C08B3708
CPC分类号： C12P7/62 , C08B11/20 , C08B37/00 , C08B37/0012 , C08B37/003 , C12P19/04
摘要： Bacillus subtilis protease catalyzes the acylation of organic solvent-insoluble polysaccharides in isooctane solution containing vinyl esters of fatty acids as acyl donor. The reaction occurs only when the enzyme is solubilized via ion-pairing with the anionic surfactant dioctyl sulfosuccinate, sodium salt (AOT). Enzyme based acylation was demonstrated with amylose, cyclodextrins, cellulose, cellulose derivatives, and other polysaccharides such as chitosan, pullulan, and maltodextrose. These polysaccharides are reactive either as a cryogenically milled powder suspended in the organic solvent or as a thin film deposited onto ZnSe slides. For chitosan, &agr;-cyclodextrin, and hydroxyethyl cellulose (HEC), the enzymatic crosslinking reaction occurs using adipic acid divinyl ester (C6DVE). HEC forms a compound that gels in solvents such as ethyl alcohol and dimethyl sulfone oxide (DMSO). Electron spectroscopy chemical analysis (ESCA) of the first 100 Å of the amylose thin film amylose indicates that the acylated surface had a degree of substitution of 0.9±0.1 acyl chains per glucose moiety and this corresponded well to the expected regioselectivity of subtilisin catalysis on glucose-containing compounds. 1H-NMR studies indicated that only the C-6 hydroxyl groups of the glucose moiety were acylated with amylose and &ggr;-cyclodextrin. However, &bgr;-cyclodextrin, and &agr;-cyclodextrin were modified at secondary alcohols and at all three alcohols, respectively. This approach represents the first attempt at using enzymes to modify organic solvent-insoluble polymers in nonaqueous media.
摘要翻译：枯草芽孢杆菌蛋白酶催化有机溶剂不溶性多糖在含有作为酰基供体的脂肪酸乙烯基酯的异辛烷溶液中的酰化。仅当酶通过离子配对与阴离子表面活性剂二辛基磺基琥珀酸钠（AOT）溶解时才发生反应。用直链淀粉，环糊精，纤维素，纤维素衍生物和其它多糖例如壳聚糖，支链淀粉和麦芽糊精证明酶基酰化。这些多糖作为悬浮在有机溶剂中的低温研磨粉末或沉积在ZnSe载片上的薄膜是反应性的。对于壳聚糖，α-环糊精和羟乙基纤维素（HEC），使用己二酸二乙烯基酯（C 6 DVE）进行酶交联反应。 HEC形成在溶剂如乙醇和二甲基砜氧化物（DMSO）中凝胶化合物。直链淀粉薄膜直链淀粉的第一个100的电子光谱化学分析（ESCA）表明，酰化表面的每个葡萄糖部分具有0.9±0.1个酰基链的取代度，这与枯草杆菌蛋白酶对葡萄糖的催化的预期区域选择性相当的化合物。 1 H-NMR研究表明，只有葡萄糖部分的C-6羟基被直链淀粉和γ-环糊精酰化。然而，β-环糊精和α-环糊精分别在仲醇和所有三种醇中进行了改性。该方法代表了使用酶来修饰非水介质中有机溶剂不溶性聚合物的第一次尝试。

32. 发明授权

US6124129A Compositions and methods for controlling genetically engineered organisms 失效
标题翻译：用于控制转基因生物的组合物和方法
公开(公告)号：US6124129A
公开(公告)日：2000-09-26
申请号：US19966
申请日：1998-02-06
申请人： Przemyslaw Szafranski , Charlene Mello , Takeshi Sano , Cassandra L. Smith , David L. Kaplan , Charles R. Cantor
发明人： Przemyslaw Szafranski , Charlene Mello , Takeshi Sano , Cassandra L. Smith , David L. Kaplan , Charles R. Cantor
IPC分类号： C07K14/36 , C12N1/21 , C12N15/72 , C12N15/74 , C12N1/20
CPC分类号： C07K14/36 , C12N15/72 , C12N15/74
摘要： Compositions and methods for the control of genetically engineered organisms are described. A more effective cell suicide approach is contemplated based on the conditional expression of the lethal Streptomyces avidinii streptavidin gene. Toxicity of streptavidin is derived from its exceptionally high binding affinity for an essential prosthetic group, D-biotin. The general requirement for biotin through the living world makes streptavidin-based conditional lethal designs applicable to a broad range of containment strategies.
摘要翻译：描述了用于控制转基因生物的组合物和方法。基于致死性链霉菌链霉亲和素链霉亲和素基因的条件表达考虑了更有效的细胞自杀方法。链霉抗生物素蛋白的毒性源自其对必需的假基团D-生物素的极高的结合亲和力。生物素通过生活世界的一般要求使基于链霉亲和素的条件致命设计适用于广泛的遏制策略。

33. 发明授权

US5993541A Process for nucleation of ceramics and product thereof 失效
标题翻译：陶瓷成核方法及其制品
公开(公告)号：US5993541A
公开(公告)日：1999-11-30
申请号：US690509
申请日：1996-07-31
申请人： Arkadi L. Litvin , Suresh Valiyaveettil , David L. Kaplan
发明人： Arkadi L. Litvin , Suresh Valiyaveettil , David L. Kaplan
IPC分类号： C30B7/00 , C30B19/12 , C30B29/16 , C30B29/24 , C30B29/58
CPC分类号： C30B29/22 , C30B29/32 , C30B7/00 , Y10S977/778
摘要： A process for controlling the formation of ceramics, achievable under ambient conditions, includes preparing a crystallization medium of a ceramic parent solution and spreading an organic monolayer of a hydrogen-bonded network on the air-liquid interface of the solution. For the formation of aragonite, the process uses an undoped calcium bicarbonate solution and a hydrogen-bonded network with a structural motif approximately matching a calcium ion distance in the a-c plane of aragonite. The aragonite product formed has a [010] axis approximately perpendicular to the monolayer. For perovskite materials, such as BSTO ceramics, the microstructure of the product is controlled by changing different functional groups of the organic monolayer which has been spread on the surface of a perovskite parent solution.
摘要翻译：用于控制在环境条件下可实现的陶瓷形成的方法包括制备陶瓷母体溶液的结晶介质并在溶液的空气 - 液体界面上铺展氢键合网络的有机单层。为了形成文石，该方法使用未掺杂的碳酸氢钙溶液和具有与文石a-c平面中钙离子距离近似匹配的结构基序的氢键网络。形成的文石产品具有大致垂直于单层的[010]轴。对于诸如BSTO陶瓷的钙钛矿材料，通过改变分散在钙钛矿母体溶液表面上的有机单层的不同官能团来控制产物的微结构。

34. 发明授权

US09808557B2 Tubular silk compositions and methods of use thereof 有权
公开(公告)号：US09808557B2
公开(公告)日：2017-11-07
申请号：US12672521
申请日：2008-08-11
申请人： Michael L. Lovett , Xianyan Wang , Christopher M. Cannizzaro , Gordana Vunjak-Novakovic , David L. Kaplan
发明人： Michael L. Lovett , Xianyan Wang , Christopher M. Cannizzaro , Gordana Vunjak-Novakovic , David L. Kaplan
IPC分类号： A61F2/06 , A61L27/36 , A61L27/22 , A61F2/08 , A61L27/26 , A61F2/07 , A61F2/82
CPC分类号： A61L27/227 , A61F2/07 , A61F2/08 , A61F2/82 , A61L27/26 , A61L27/3604 , D10B2211/22 , Y10T428/13 , C08L89/00
摘要： Described are tubular silk fibroin compositions and methods for their manufacture and use. Tubular compositions as described herein can be produced in a range of high burst strengths, can easily be made in a range of inner diameters, can be derivatized with functional moieties, and can be produced in a range of permeabilities suitable for particularized uses. In one aspect, the tubular compositions can be used in the repair or replacement of damaged or diseased blood vessels, including, but not limited to vessels smaller than about 6 mm.

35. 发明授权

US09603971B2 Silk-based ionomeric compositions 有权
公开(公告)号：US09603971B2
公开(公告)日：2017-03-28
申请号：US13582903
申请日：2011-03-04
申请人： David L. Kaplan , Monica A. Serban
发明人： David L. Kaplan , Monica A. Serban
IPC分类号： A61L27/52 , A61K9/08 , A61K9/50 , A61L15/26 , A61L15/32 , A61L27/18 , A61L27/22 , A61L27/26 , A61L27/50 , A61L27/54 , A61L15/42
CPC分类号： A61L27/52 , A61K9/5063 , A61L15/26 , A61L15/32 , A61L15/42 , A61L27/18 , A61L27/227 , A61L27/26 , A61L27/50 , A61L27/54 , A61L2300/00 , Y10T428/2982 , C08L77/04
摘要： Disclosed herein are pH-dependent silk fibroin-based ionomeric compositions and colloids, and methods of making the same. The state of the silk fibroin ionomeric compositions is reversible and can transform from a gel-like colloid to a more fluid-like solution, or vice versa, upon an environmental stimulus, e.g., pH. Thus, the silk-based ionomeric compositions and colloids can be applied in various industries, ranging from electronic applications to biomedical applications, such as sensors, gel diodes, absorbent materials, drug delivery systems, tissue implants and contrast agents.

36. 发明授权

US09585830B2 Vitamin C composition for use in the prevention and treatment of stretch marks, radiation dermatitis, and other skin conditions and methods of using the same 有权
标题翻译：用于预防和治疗妊娠纹，放射性皮炎和其他皮肤病症的维生素C组合物及其使用方法
公开(公告)号：US09585830B2
公开(公告)日：2017-03-07
申请号：US13738125
申请日：2013-01-10
申请人： David L. Kaplan
发明人： David L. Kaplan
IPC分类号： A01N43/08 , A61K31/34 , A61K8/67 , A61K9/00 , A61K47/10 , A61Q19/06 , A61K31/341 , A61Q19/00
CPC分类号： A61K8/676 , A61K9/0014 , A61K31/34 , A61K31/341 , A61K47/10 , A61Q19/004 , A61Q19/06
摘要： A formulation for a stable ascorbic acid composition which, in a simplified form, is comprised of ascorbic acid in solution with a hygroscopic compound (i.e., a substance with the ability to attract water molecules from the surrounding environment through either absorption or adsorption). Also disclosed herein are methods for the production of such compounds and methods of using such compounds in the prevention, inhibition and treatment of striae gravidarum, radiation dermatitis, rhytids, lentigoes, dyschromia, sun-damage induced hyperpigmentation, cellulite, scars and purpura, among other skin diseases or conditions.
摘要翻译：用于稳定的抗坏血酸组合物的制剂，其以简单的形式由溶液中的抗坏血酸与吸湿性化合物（即具有通过吸收或吸附从周围环境吸引水分子的能力的物质）组成。本文还公开了生产这些化合物的方法和使用这些化合物在预防，抑制和治疗妊娠纹，放射性皮炎，ids ids，，，es，dys ia ia ia，，，damage damage ite，among among among among among among among among among among among among among among among among among among 其他皮肤病或病症。

37. 发明授权

US09566365B2 Silk fibroin and polyethylene glycol-based biomaterials 有权
标题翻译：丝素蛋白和聚乙二醇基生物材料
公开(公告)号：US09566365B2
公开(公告)日：2017-02-14
申请号：US13820440
申请日：2011-09-01
申请人： David L. Kaplan , Monica A. Serban
发明人： David L. Kaplan , Monica A. Serban
IPC分类号： A61L24/10 , C08L77/04 , C08L71/02 , C08L89/00 , A61L27/26 , A61L27/52 , A61L27/54 , A61L24/00
CPC分类号： A61L24/108 , A61L24/0094 , A61L27/26 , A61L27/52 , A61L27/54 , A61L2300/406 , C08L71/02 , C08L77/04 , C08L89/00 , C08L89/06
摘要： This invention relates to methods and compositions for preparation of silk-PEGs based biomaterials through crosslinking by chemically reacting active polyethylene glycols (PEGs) possessing different chemical groups (e.g., thiols and maleimides functionalized PEGs) that are additionally stabilized by the beta-sheet formation of silk fibroin. The crosslinked silk-PEGs biomaterials present strong adhesive properties, which are comparable to or better than the current leading PEG-based sealant, depending on the silk concentration in the silk-PEGs biomaterials. In addition, the silk-PEGs based biomaterials are cytocompatible, show decreased swelling behavior and longer degradation times, which make them suitable for hemostatic applications where the current available tissue sealant products can be contraindicated.
摘要翻译：本发明涉及通过化学反应具有不同化学基团的活性聚乙二醇（PEG）（例如硫醇和马来酰亚胺官能化的PEG）进行交联而制备基于丝-PEG的生物材料的方法和组合物，其通过β- 丝素蛋白交联的丝-PEGs生物材料具有强大的粘合性能，其与当前领先的PEG基密封剂相当或更好，这取决于丝-PEG生物材料中的丝浓度。此外，基于丝-PEG的生物材料是细胞相容性的，显示出降低的溶胀行为和更长的降解时间，这使得它们适用于当前可用的组织密封剂产品可以禁忌的止血应用。

38. 发明授权

US09427499B2 Surface modification of silk fibroin matrices with poly(ethylene glycol) useful as anti-adhesion barriers and anti-thrombotic materials 有权
标题翻译：用聚乙二醇作为抗粘连屏障和抗血栓形成材料的丝素蛋白基质的表面改性
公开(公告)号：US09427499B2
公开(公告)日：2016-08-30
申请号：US13129738
申请日：2009-11-17
申请人： Charu P. Vepari , David L. Kaplan
发明人： Charu P. Vepari , David L. Kaplan
IPC分类号： A61L27/14 , A61L27/28 , A61L27/22 , A61L31/10 , A61L33/06 , A61L31/00
CPC分类号： A61L31/005 , A61L31/10 , A61L33/068 , C08L71/02
摘要： The present invention provides compositions and methods for the production of silk fibroin matrices surface-PEGylated on one or more surfaces. Such surface-PEGylated silk fibroin matrices can be used in biomedical applications, such as anti-adhesive and anti-thrombosis materials. Silk matrices may be surface-PEGylated, for example, by a reaction with a functional group-activated PEG. Controlling the degree of PEGylation on surface of silk fibroin matrix can regulate both the degradation rate of the silk matrix, and the differentiated adhesion of cells or differentiated adsorption of proteins on the surface of the silk matrix. The present invention also provides for silk fibroin matrices having one or more surfaces possessing differentiated adhesion properties, which allows for tissue integration on the adherent side and inhibition of tissue adhesion to the opposing tissues or organs. Embedding active agents in silk fibroin matrices provides more benefits, such as promoting tissue ingrowth on the adherent side of the matrix.
摘要翻译：本发明提供了用于生产在一个或多个表面上表面聚乙二醇化的丝素蛋白基质的组合物和方法。这种表面聚乙二醇化的丝素蛋白基质可用于生物医学应用，例如抗粘连和抗血栓形成的材料。丝质基质可以被表面聚乙二醇化，例如通过与官能团活化的PEG的反应。控制丝素蛋白基质表面聚乙二醇化程度可以调节蚕丝基质的降解速率，分化细胞粘附或蛋白质在丝质基质表面的分化吸附。本发明还提供具有一个或多个具有分化的粘附性质的表面的丝素蛋白基质，其允许组合物在粘附层上的整合并抑制组织对相对组织或器官的粘附。在丝素蛋白基质中包埋活性剂提供了更多的益处，例如促进组织向上生长在基质的粘着面上。

39. 发明授权

US09074302B2 Methods of making drawn silk fibers 有权
标题翻译：制作拉丝丝纤维的方法
公开(公告)号：US09074302B2
公开(公告)日：2015-07-07
申请号：US13498722
申请日：2010-09-28
申请人： Tim Jia-Ching Lo , Gary G. Leisk , David L. Kaplan
发明人： Tim Jia-Ching Lo , Gary G. Leisk , David L. Kaplan
IPC分类号： C08L89/00 , D01D5/40 , D01F4/00 , D01F4/02 , D01D1/02 , D01D5/00 , G02B6/02
CPC分类号： D01F4/02 , D01D1/02 , D01D5/00 , G02B6/02033
摘要： The present invention relates to compositions and method for drawing egel silk fibroin fibers. The resulting fibers can transmit light and hence can be used as optical fiber. Silk fibroin fiber is produced by a method comprising applying an electric field to a solubilized silk fibroin solution to create a silk fibroin gel; converting the silk fibroin gel to a viscous silk liquid; and drawing a silk fiber from the viscous silk liquid. The silk fiber of the invention can be used in materials such as textile, medical sutures, and tissue materials, as well as conferring optical properties into these materials.
摘要翻译：本发明涉及丝绒丝素纤维的组合物和方法。所得到的纤维可以透射光，因此可以用作光纤。丝素蛋白纤维通过包括向溶解的丝素蛋白溶液施加电场以产生丝素蛋白凝胶的方法产生; 将丝素蛋白凝胶转化为粘稠的丝液; 并从粘性丝液中抽出丝纤维。本发明的丝纤维可以用于纺织品，医用缝合线和组织材料等材料，并且可以赋予这些材料光学特性。

40. 发明授权

US09016875B2 All-protein implantable, resorbable reflectors 有权
标题翻译：全蛋白可植入，可吸收反射体
公开(公告)号：US09016875B2
公开(公告)日：2015-04-28
申请号：US13386388
申请日：2010-07-20
申请人： Fiorenzo Omenetto , David L. Kaplan
发明人： Fiorenzo Omenetto , David L. Kaplan
IPC分类号： G02B5/124 , A61B5/00 , A61L27/22
CPC分类号： A61B5/0059 , A61K49/0004 , A61L27/227
摘要： The invention provides for compositions and process for fabricating an optical reflector constructed from biocompatible and bioresorbable silk fibroin proteins. For example, the silk retroreflectors may be built based on millimeter size microprism arrays to rotate the image plane of imaged cortical layers, thus enhancing the amount of photons that are detectable in the reflected direction when inserted in a sample to be analyzed, and ultimately increasing in contrast ratio in multiphoton microscopy. Such device can be used as a label-free, biocompatible, bioresorbable, implantable device for various applications ranging from medical imaging/diagnostics, drug/therapeutic delivery, to food chain safety and environmental monitoring.
摘要翻译：本发明提供了用于制造由生物相容性和生物可再吸收的丝素蛋白构成的光反射体的组合物和方法。例如，丝绸后向反射器可以基于毫米尺寸的微棱镜阵列构建，以旋转成像的皮质层的图像平面，从而增加当插入待分析的样品中时在反射方向上可检测的光子的量，并且最终增加在多光子显微镜中的对比度。这种装置可用作用于各种应用的无标签，生物相容性，生物可再吸收的可植入装置，从医学成像/诊断，药物/治疗递送，食品链安全和环境监测。

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