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31. 发明申请

US20100172968A1 CHIMERIC CANNULAE PROTEINS AND METHODS FOR MAKING AND USING THEM 审中-公开
标题翻译： CHIMERIC CANNULAE蛋白质及其制备和使用方法
公开(公告)号：US20100172968A1
公开(公告)日：2010-07-08
申请号：US11929781
申请日：2007-10-30
申请人： Jay Short , Eric J. Mathur , W. Michael Lafferty , Nelson Barton , Kevin Chow
发明人： Jay Short , Eric J. Mathur , W. Michael Lafferty , Nelson Barton , Kevin Chow
IPC分类号： A61K9/48 , A61K9/10 , C12N15/63 , C07K14/00 , C07K16/00
CPC分类号： B82Y30/00 , A61K9/0092 , A61K9/1274 , C07K14/195
摘要： A polymer is prepared by self-assembly of a plurality of monomeric polypeptide units. The polymer tends to form a nanotube and is capable of encapsulating a particular drug molecule. Once encapsulated in the polymer of the present invention, the drug molecule may be delivered to a particular location of human body to effectively cure a disease or treat a symptom.Generally, the monomeric polypeptide unit of the present invention has a sequence found in Pyrodictium abyssi, a microorganism that produces an extracellular network having hollow protein tubes, or a sequence substantially identical thereto. The monomeric polypeptide may be mass produced using recombinant biotechnologies and be polymerized into the polymer of the present invention. One or more additional targeting vector may be attached to the monomeric polypeptide unit or the polymer to facilitate the targeting of the drug molecule that may be held there within. The sequence contained in the monomeric polypeptide unit may be further optimized using one or more technique selected from Gene Site Saturation Mutagenesis and GeneReasembly.
摘要翻译：通过多个单体多肽单元的自组装制备聚合物。聚合物倾向于形成纳米管并且能够封装特定的药物分子。一旦包封在本发明的聚合物中，药物分子可以被递送到人体的特定位置以有效治愈疾病或治疗症状。通常，本发明的单体多肽单元具有在黑腹果蝇（Pyrodictium abyssi）中发现的序列，该菌株产生具有中空蛋白管的细胞外网络或与其基本相同的序列。单体多肽可以使用重组生物技术大规模生产并聚合成本发明的聚合物。一个或多个另外的靶向载体可以连接到单体多肽单元或聚合物，以促进可能在其中保持的药物分子的靶向。可以使用选自基因位点饱和诱变和基因重组的一种或多种技术进一步优化单体多肽单元中所含的序列。

32. 发明申请

US20050142658A1 Chromosomal saturation mutagenesis 审中-公开
标题翻译：染色体饱和诱变
公开(公告)号：US20050142658A1
公开(公告)日：2005-06-30
申请号：US10494875
申请日：2002-12-03
申请人： Jay Short , Michelle Cayouette
发明人： Jay Short , Michelle Cayouette
IPC分类号： C12N15/09 , C12N20060101 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N15/00 , C12N15/10 , C12Q1/68
CPC分类号： C12N15/102
摘要： In One aspect, the invention provides methods for Chromosomal Saturation Mutagenesis (CSM) comprising generating randomly mutated, overlapping segments for an entire chromosome using error-prone PCR or other techniques, and libraries of nucleic acids made by these methods.
摘要翻译：一方面，本发明提供了染色体饱和诱变（CSM）的方法，其包括使用易错PCR或其他技术为整个染色体生成随机突变的重叠片段，以及通过这些方法制备的核酸文库。

33. 发明申请

US20050064498A1 High throughput screening for sequences of interest 审中-公开
标题翻译：感兴趣的序列的高通量筛选
公开(公告)号：US20050064498A1
公开(公告)日：2005-03-24
申请号：US10960137
申请日：2004-10-07
申请人： Jay Short , Martin Keller
发明人： Jay Short , Martin Keller
IPC分类号： C12N1/12 , C12N1/20 , C12Q1/68 , G01N33/554 , G01N33/569
CPC分类号： C12N15/1034 , C12N1/20 , C12N11/04 , C12N15/1086 , C12Q1/04 , C40B40/08 , C40B50/06 , G01N33/56911
摘要： Provided is a method of screening or enriching a sample containing polynucleotides from a mixed population of organisms. The method includes creating a DNA library from a plurality of nucleic acid sequences of a mixed population of organisms and separating clones containing a polynucleotide sequence of interest on a fluorescence analyzer based upon hybridization of a fluorescence labeled oligonucleotide probe to a target sequence in a clone. The separated or enrich library can then be further process by activity based screening or sequence based screening. In addition, the enriched sequence can be compared to a database and to identify sequences in the database which have homology to a clone in the library thereby obtaining a nucleic acid profile of the mixed population of organisms.
摘要翻译：提供了筛选或富集来自生物体的混合群体的含有多核苷酸的样品的方法。该方法包括基于荧光标记的寡核苷酸探针与克隆中的靶序列的杂交，在荧光分析仪上从多个生物体的多个核酸序列产生DNA文库和分离含有多核苷酸序列的克隆。然后可以通过基于活性的筛选或基于序列的筛选进一步处理分离或富集的文库。此外，可将富集的序列与数据库进行比较，并鉴定数据库中与文库中的克隆具有同源性的序列，从而获得混合生物群体的核酸谱。

34. 发明申请

US20050009080A1 Production of enzymes having desired activities by mutagenesis 审中-公开
标题翻译：通过诱变生产具有所需活性的酶
公开(公告)号：US20050009080A1
公开(公告)日：2005-01-13
申请号：US10912465
申请日：2004-08-04
申请人： Jay Short
发明人： Jay Short
IPC分类号： C12N9/00 , C12N9/16 , C12N9/24 , C12N15/10 , C12Q1/68 , C12N9/26
CPC分类号： C12N9/00 , C12N9/16 , C12N9/2445 , C12N15/102 , C12N15/1034 , C12Y301/03001 , C12Y302/01021
摘要： Disclosed is a process for obtaining an enzyme having a specified enzyme activity derived from a heterogeneous DNA population by screening, for the specified enzyme activity, a library of clones containing DNA from the heterogeneous DNA population which have been exposed to directed mutagenesis towards production of the specified enzyme activity. Also disclosed is a process for obtaining an enzyme having a specified enzyme activity by screening, for the specified enzyme activity, a library of clones containing DNA from a pool of DNA populations which have been exposed to directed mutagenesis in an attempt to produce in the library of clones DNA encoding an enzyme having one or more desired characteristics which can be the same or different from the specified enzyme activity.
摘要翻译：公开了一种获得具有来自异质DNA群体的特定酶活性的酶的方法，对于规定的酶活性，筛选含有已经暴露于定向诱变的异源DNA群体的DNA的克隆的文库，指定的酶活性。还公开了获得具有特定酶活性的酶的方法，通过筛选已经暴露于定向诱变的DNA群体中含有DNA的克隆的特定酶活性文库，试图在文库中产生的克隆DNA，其编码具有一个或多个可以与指定酶活性相同或不同的所需特征的酶。

35. 发明申请

US20110229451A2 TEMPERATURE SENSITIVE MUTANTS OF MATRIX METALLOPROTEASES AND USES THEREOF 审中-公开
标题翻译：基质金属的温度敏感性及其用途
公开(公告)号：US20110229451A2
公开(公告)日：2011-09-22
申请号：US12660894
申请日：2010-03-05
申请人： Louis Bookbinder , Gregory Frost , Gilbert Keller , Gerhard Frey , Hwai Chang , Jay Short
发明人： Louis Bookbinder , Gregory Frost , Gilbert Keller , Gerhard Frey , Hwai Chang , Jay Short
IPC分类号： A61K38/48 , A61K38/47 , A61P17/02 , A61P19/00 , A61P17/00 , C12N9/50 , C12N9/96 , C07H21/04 , C12N15/63 , C12N5/10
CPC分类号： C12N9/6491 , A61K38/4886 , C12Y304/24007
摘要： Provided are modified matrix metalloprotease (MMP) enzymes that exhibit temperature-dependent activity and uses thereof. The MMPs can be used, for example, to treat ECM-mediated diseases or disorders characterized by increased deposition or accumulation of one or more ECM components.
摘要翻译：提供了显示温度依赖性活性及其用途的修饰的基质金属蛋白酶（MMP）酶。 MMP可用于例如治疗ECM介导的疾病或特征在于增加一种或多种ECM组分的沉积或积累的疾病。

36. 发明申请

US20060088821A1 Construction and use of catalogued nucleic acid libraries that contain advantageously adjusted representations of defined components 审中-公开
公开(公告)号：US20060088821A1
公开(公告)日：2006-04-27
申请号：US10034622
申请日：2001-12-21
申请人： Jay Short
发明人： Jay Short
IPC分类号： C40B40/08 , C12Q1/68
CPC分类号： C07H21/00 , C12N15/1093 , C40B40/00 , C40B40/08
摘要： A process for constructing a catalogued nucleic acid library in which the proportional representation of the constituents is adjusted to advantage through the use of disclosed technologies for positive and negative selection. The resultant benefit is that significantly fewer library constituents will need to be screened in order to identify a potentially desired constituent. Moreover, library constituents that previously would have been essentially “lost” are now recoverable. Preferred embodiments of this invention include the cataloguing, normalization, and enrichment of library constituents. By way of example, but not limitation, this technology is serviceable for constructing a library that contains an adequate representation of desirable constituents that (1) are initially found in low-copy numbers within a sample source or (2) originate from an organism that is problematic to culture. Applicable uses of this invention include any library-screening endeavor previously hindered by logistical impediments. By expanding previous logistical frontiers this invention allows for a novel generation of previously unattainable molecules—particularly molecules that are “unclonable” from conventional, unadjusted libraries—to now be detected, cloned, manipulated, expressed, studied, and used. By disclosing the construction and screening of high yielding nucleic acid libraries from mixed and uncultivated organisms, the instant technology eclipses former boundaries in the area of biological discovery and enables the full breadth of biological diversity to be accessed in the search for previously undiscovered genes and gene products. The benefits of the present invention are seen to extend to areas of diagnosis, medicine, agriculture, manufacturing, and academia.

37. 发明申请

US20050246780A1 Phytases, nucleic acids encoding them and methods for making and using them 有权
标题翻译：植酸酶，编码它们的核酸和制备和使用它们的方法
公开(公告)号：US20050246780A1
公开(公告)日：2005-11-03
申请号：US11056354
申请日：2005-02-11
申请人： Jay Short , Keith Kretz , Kevin Gray , Nelson Barton , James Garrett , Eileen O'Donoghue , Eric Mathur
发明人： Jay Short , Keith Kretz , Kevin Gray , Nelson Barton , James Garrett , Eileen O'Donoghue , Eric Mathur
IPC分类号： A01H1/00 , A01K67/00 , A21D8/04 , A23J3/14 , A23K1/14 , A23K1/165 , A23L1/015 , A23L1/03 , A23L1/105 , A23L1/211 , A61K38/00 , C07H21/04 , C12N1/21 , C12N5/04 , C12N9/16 , C12N15/82
CPC分类号： A21D8/042 , A01K2217/05 , A23J3/14 , A23K10/30 , A23K20/189 , A23L5/25 , A23L7/107 , A23L11/33 , A23L29/06 , A23V2002/00 , A61K38/00 , C12N9/16 , C12Y301/03008 , C12Y301/03026 , A23V2300/21
摘要： The invention provides isolated and recombinant phytase enzymes. In one aspect, the phytases are produced by modification of the wild type appA of E. coli. The enzyme can be produced from recombinant host cells. The phytases of the invention can be used to aid in the digestion of phytate where desired. In particular, the phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be thermotolerant and/or thermostable. Also provided are methods for obtaining a variant polynucleotide encoding a phytase and for obtaining a phytase with thermostability or thermotolerant at high or low temperatures.
摘要翻译：本发明提供了分离和重组的植酸酶。一方面，通过修饰大肠杆菌的野生型appA产生植酸酶。该酶可以从重组宿主细胞产生。本发明的植酸酶可以用于有助于在需要时消化植酸盐。特别地，本发明的植酸酶可用于食品中以改善富含植酸盐的成分的摄食量。本发明的植酸酶可以是耐热和/或耐热的。还提供了获得编码植酸酶的变体多核苷酸和在高温或低温下获得具有热稳定性或耐热性的植酸酶的方法。

38. 发明申请

US20050208515A1 Method of DNA shuffling with polynucleotides produced by blocking or interrupting a synthesis or amplification process 审中-公开
标题翻译：通过阻断或中断合成或扩增过程产生的多核苷酸进行DNA改组的方法
公开(公告)号：US20050208515A1
公开(公告)日：2005-09-22
申请号：US10981044
申请日：2004-11-04
申请人： Jay Short
发明人： Jay Short
IPC分类号： C12N15/10 , C12P19/34 , C12Q1/68 , C12Q1/6811 , C12Q1/6844
CPC分类号： C12N15/1027 , C12Q1/6811 , C12Q1/6844 , C12Q2600/156 , C12Q2525/179 , C12Q2523/313
摘要： Disclosed is a process of performing “sexual” PCR which includes generating random polynucleotides by interrupting or blocking a synthesis or amplification process to show or halt synthesis or amplification of at least one polynucleotide, optionally amplifying the polynucleotides, and reannealing the polynucleotides to produce random mutant polynucleotides. Also provided are vector and expression vehicles including such mutant polynucleotides, polypeptides expressed by the mutant polynucleotides and a method for producing random mutant polypeptides.
摘要翻译：披露了进行“性”PCR的过程，其包括通过中断或阻断合成或扩增过程产生随机多核苷酸，以显示或停止至少一种多核苷酸的合成或扩增，任选地扩增多核苷酸，以及重新退火多核苷酸以产生随机突变体多核苷酸。还提供了载体和表达载体，包括这种突变多核苷酸，由突变多核苷酸表达的多肽和用于产生随机突变多肽的方法。

39. 发明申请

US20050202494A1 Catalases 审中-公开
标题翻译：过氧化氢酶
公开(公告)号：US20050202494A1
公开(公告)日：2005-09-15
申请号：US11096819
申请日：2005-04-01
申请人： Jay Short , Dan Robertson , Indrajit Sanyal , Robert Adhikari , Eric Mathur
发明人： Jay Short , Dan Robertson , Indrajit Sanyal , Robert Adhikari , Eric Mathur
IPC分类号： C12N1/15 , C12N1/19 , C12N1/21 , C12N9/08 , C12Q1/30 , C12Q1/68 , C07H21/04 , C12N15/09
CPC分类号： C12N9/0065 , A01K2217/05 , C12Q1/30
摘要： The invention relates to catalases and to polynucleotides encoding the catalases. In addition methods of designing new catalases and method of use thereof are also provided. The catalases have increased activity and stability at increased pH and temperature.
摘要翻译：本发明涉及过氧化氢酶和编码该过氧化氢酶的多核苷酸。另外还提供了设计新的过氧化氢酶的方法及其使用方法。过氧化氢酶在增加的pH和温度下具有增加的活性和稳定性。

40. 发明申请

US20050130156A1 Methods for making polynucleotides and purifying double-stranded polynucleotides 审中-公开
标题翻译：制备多核苷酸和纯化双链多核苷酸的方法
公开(公告)号：US20050130156A1
公开(公告)日：2005-06-16
申请号：US10501592
申请日：2003-01-14
申请人： Gerhard Frey , Jay Short , Lilian Parra-Gessert
发明人： Gerhard Frey , Jay Short , Lilian Parra-Gessert
IPC分类号： A01K67/027 , B01J20/281 , C07H21/04 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N15/09 , C12N15/10 , C12P21/08 , C12Q1/68 , G01N30/88
CPC分类号： C12Q1/6806 , C12Q2521/514 , C12Q2563/131
摘要： The invention provides methods for identifying and purifying double-stranded polynucleotides lacking base pair mismatches, insertion/deletion loops and/or nucleotide gaps. The invention provides libraries of nucleic acid building blocks and methods for generating any nucleic acid sequence, including synthetic genes, antisense constructs and polypeptide coding sequences. The invention provides chimeric antigen binding molecules and the nucleic acids that encode them.
摘要翻译：本发明提供了鉴定和纯化缺乏碱基对失配，插入/缺失环和/或核苷酸间隙的双链多核苷酸的方法。本发明提供了核酸构建块的文库和用于产生任何核酸序列的方法，包括合成基因，反义构建体和多肽编码序列。本发明提供嵌合抗原结合分子和编码它们的核酸。

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