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    • 39. 发明申请
    • VIRAL PARTICLES WHICH ARE MASKED OR UNMASKED WITH RESPECT TO A CELL RECEPTOR
    • 与细胞受体相关的病毒颗粒被隐藏或被忽略
    • WO1997044474A2
    • 1997-11-27
    • PCT/FR1997000870
    • 1997-05-16
    • CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUECOSSET, François-LoîcVALSESIA, SandrineRUSSELL, Stephen, J.
    • CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
    • C12N15/86
    • C07K14/005A61K47/6901A61K48/00C07K1/1075C07K14/485C07K2319/00C12N7/00C12N15/62C12N15/86C12N15/87C12N2740/13022C12N2740/13043C12N2740/13045C12N2740/13062C12N2810/60C12N2810/6018C12N2810/6054C12N2810/80C12N2810/851C12N2810/852C12N2810/854
    • The invention features the use of a peptide for transferring genes in a eukaryotic target cell, which peptide has about 10 to about 200, in particular about 15 to about 150 amino acids, and advantageously about 20 amino acids, in which 30 % at least of the amino acids are constituted by proline radicals, which proline radicals are regularly arranged so as to induce polypeptide chain turnings at about 180 DEG (" beta -turn" or "reverse-turn"), these turns being regularly spaced and gathering in a polyproline beta -turn helix, in a polypeptide construction containing, on the said peptide N-terminal side (upstream), an N-terminal (upstream) proteinic domain capable of recognising a targeted surface molecule or an antigen expressed on a cellular surface, in particular an appropriate receptor (targeted receptor) located on the said eukaryotic cell, and on the said peptide C-terminal side (downstream), a C-terminal (downstream) protein domain capable of recognising an appropriate receptor (auxiliary receptor) located on the said eukaryotic cell, which peptide is capable of facilitating or inhibiting interaction between the C-terminal (downstream) protein domain and the auxiliary receptor, the inhibition of this interaction taking place so long as the N-terminal (upstream) protein domain has not interacted with the targeted receptor, and the facilitating of the interaction between the C-terminal (downstream) protein domain and the auxiliary receptor taking place when the N-terminal (upstream) protein domain has interacted with the targeted receptor.
    • 本发明的特征在于使用肽在真核靶细胞中转移基因,该肽具有约10至约200,特别是约15至约150个氨基酸,并且有利地约20个氨基酸,其中至少30% 氨基酸由脯氨酸基团构成,脯氨酸自由基被规则排列,以便在约180°(“β转”或“反转”)处诱导多肽链转角,这些转动规则地间隔开并聚集在聚脯氨酸 特别是在所述肽N-末端侧(上游)上含有能够识别目标表面分子或表达于细胞表面的抗原的N末端(上游)蛋白质结构域的多肽构建体中,β转角螺旋 位于所述真核细胞上的适当受体(靶向受体)和所述肽C-末端侧(下游),能够识别合适受体的C末端(下游)蛋白结构域(a 位于所述真核细胞上的所述肽,所述肽能够促进或抑制C-末端(下游)蛋白质结构域和辅助受体之间的相互作用,只要N末端(上游 )蛋白质结构域没有与靶向受体相互作用,并且当N-末端(上游)蛋白质结构域与靶向受体相互作用时,C末端(下游)蛋白质结构域和辅助受体之间的相互作用的促进发生。