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31. 发明授权

US07471817B2 Method for performing interactions on microscopic subjects that change in space and time, and system therefor 有权
标题翻译：在空间和时间上改变的微观科目上执行相互作用的方法及其系统
公开(公告)号：US07471817B2
公开(公告)日：2008-12-30
申请号：US10809960
申请日：2004-03-26
申请人： Frank Olschewski , Volker Seyfried
发明人： Frank Olschewski , Volker Seyfried
IPC分类号： G06K9/00
CPC分类号： G06T7/0012 , G06K9/00127
摘要： The method and the system simplify moving interactions by means of virtual reference subjects and flux-based coordinate transformations in order to generate a changeable frame of reference.
摘要翻译：该方法和系统通过虚拟参考对象和基于通量的坐标变换简化移动交互，以便生成可变的参考框架。

32. 发明申请

US20070200052A1 Apparatus, microscope with an apparatus, and method for calibration of a photosensor chip 有权
标题翻译：装置，具有装置的显微镜和用于校准光电传感器芯片的方法
公开(公告)号：US20070200052A1
公开(公告)日：2007-08-30
申请号：US11649877
申请日：2007-01-05
申请人： Volker Seyfried , Frank Schreiber
发明人： Volker Seyfried , Frank Schreiber
IPC分类号： G02B27/40
CPC分类号： H01L27/14806 , G01J1/02 , G01J1/0295 , G01J1/04 , G01J1/0414 , G01J1/0437 , G01J1/0444 , G01J1/0488 , G01J1/08 , G01J1/10
摘要： An apparatus, a microscope having an apparatus, and a method for calibration of a photosensor chip (19) are disclosed. The apparatus has a photosensor chip (19) which has a multiplicity of light-sensitive elements. A reference light source (30) is provided and directs the light at at least one part of the photosensor chip (19). In addition, an open-loop or closed-loop control unit (19a) is provided and determines and corrects variances between the individual light-sensitive elements.
摘要翻译：公开了一种装置，具有装置的显微镜和用于校准光传感器芯片（19）的方法。该装置具有光传感器芯片（19），其具有多个感光元件。提供参考光源（30）并将光引导到光传感器芯片（19）的至少一部分。此外，提供开环或闭环控制单元（19a），并确定和校正各个感光元件之间的差异。

33. 发明授权

US08293490B2 Method for high spatial resolution stochastic examination of a sample structure labeled with a substance 有权
标题翻译：用物质标记的样品结构的高空间分辨率随机检验方法
公开(公告)号：US08293490B2
公开(公告)日：2012-10-23
申请号：US12632639
申请日：2009-12-07
申请人： Volker Seyfried , Jochen Sieber
发明人： Volker Seyfried , Jochen Sieber
IPC分类号： G01N33/53 , G01N31/00
CPC分类号： G01N21/6428 , G01N21/6458
摘要： A method for high spatial resolution stochastic examination of a biological sample structure labeled with a labeling substance is described. The method comprises providing a biological sample structure; choosing such a labeling substance that has molecules present in a first state and in a second state, and the first and second states differ from one another in at least one photophysical property such that there is sufficient probability that one portion of the molecules of the substance will be in the first state and another portion of the molecules will be in the second state and within which labeling substance a change of the state of the molecules can occur spontaneously between the two states in both directions; and labeling the biological sample structure with the substance.
摘要翻译：描述了用标记物质标记的生物样品结构的高空间分辨率随机检查方法。该方法包括提供生物样品结构; 选择具有以第一状态和第二状态存在的分子的这种标记物质，并且第一和第二状态在至少一种光物理性质上彼此不同，使得物质分子的一部分有足够的可能性将处于第一状态，并且分子的另一部分将处于第二状态，并且标记物质内的分子状态的变化可以在两个方向的两个状态之间自发地发生; 并用物质标记生物样品结构。

34. 发明申请

US20110279890A1 METHOD AND DEVICE FOR ILLUMINATING A SAMPLE IN A LASER MICROSCOPE 有权
标题翻译：用于在激光显微镜中照射样品的方法和装置
公开(公告)号：US20110279890A1
公开(公告)日：2011-11-17
申请号：US12674343
申请日：2008-08-11
申请人： Volker Seyfried , Hilmar Gugel , Carsten L. Thomsen
发明人： Volker Seyfried , Hilmar Gugel , Carsten L. Thomsen
IPC分类号： H01S3/063 , G02B21/06
CPC分类号： G02B21/0032
摘要： The invention relates to a method and a device for illuminating or irradiating an object, a sample (8), or the like, for the purpose of imaging or analysis, particularly for use in a laser microscope (1), preferably in a confocal microscope having a laser light source (2) emitting the illuminating light, the laser light being coupled directly or by means of a glass fiber into an illumination light path (4), characterized in that the laser light source (2) is switched on rapidly upon a trigger signal directly prior to the actual need, for example, directly prior to imaging.
摘要翻译：本发明涉及一种用于照射或照射物体的方法和装置，用于成像或分析的样品（8）等，特别是用于激光显微镜（1）中，优选在共聚焦显微镜具有发射所述照明光的激光源（2），所述激光直接或通过玻璃纤维耦合到照明光路（4）中，其特征在于，所述激光源（2）被快速地接通直接在实际需要之前的触发信号，例如直接在成像之前。

35. 发明授权

US07830506B2 Method for high spatial resolution examination of samples 有权
标题翻译：样品高空间分辨率检测方法
公开(公告)号：US07830506B2
公开(公告)日：2010-11-09
申请号：US11653444
申请日：2007-01-16
申请人： Hilmar Gugel , Marcus Dyba , Volker Seyfried
发明人： Hilmar Gugel , Marcus Dyba , Volker Seyfried
IPC分类号： G01J3/30
CPC分类号： G01N21/6458
摘要： A method for high spatial resolution examination of a sample, the sample to be examined including a substance that can be repeatedly converted from a first state into a second state, the first and the second states differing from one another in at least one optical property. The method includes: a) bringing the substance into the first state by means of a switching signal in a sample region to be recorded, b)inducing the second state by means of an optical signal, spatially delimited subregions being specifically excluded within the sample region to be recorded, c) reading out the remaining first states, and d) steps a) to c) are repeated, the optical signal being displaced upon each repetition in order to scan the sample, wherein the individual steps a) to d) are carried out in a sequence adapted to the respective measuring situation.
摘要翻译：一种用于样品的高空间分辨率检查的方法，待检查的样品包括可以从第一状态重复转换为第二状态的物质，第一和第二状态在至少一个光学性质中彼此不同。该方法包括：a）借助于要记录的样本区域中的切换信号使物质进入第一状态，b）通过光信号诱导第二状态，在样本区域内具体排除空间划分的子区域被记录，c）读出剩余的第一状态，并且d）步骤a）至c）被重复，光信号在每次重复时被移位以便扫描样品，其中各个步骤a）至d）是以适合于相应测量情况的顺序进行。

36. 发明授权

US07576316B2 Apparatus, microscope with an apparatus, and method for calibration of a photosensor chip 有权
标题翻译：装置，具有装置的显微镜和用于校准光电传感器芯片的方法
公开(公告)号：US07576316B2
公开(公告)日：2009-08-18
申请号：US11649877
申请日：2007-01-05
申请人： Volker Seyfried , Frank Schreiber
发明人： Volker Seyfried , Frank Schreiber
IPC分类号： G12B13/00
CPC分类号： H01L27/14806 , G01J1/02 , G01J1/0295 , G01J1/04 , G01J1/0414 , G01J1/0437 , G01J1/0444 , G01J1/0488 , G01J1/08 , G01J1/10
摘要： An apparatus, a microscope having an apparatus, and a method for calibration of a photosensor chip (19) are disclosed. The apparatus has a photosensor chip (19) which has a multiplicity of light-sensitive elements. A reference light source (30) is provided and directs the light at at least one part of the photosensor chip (19). In addition, an open-loop or closed-loop control unit (19a) is provided and determines and corrects variances between the individual light-sensitive elements.
摘要翻译：公开了一种装置，具有装置的显微镜和用于校准光传感器芯片（19）的方法。该装置具有光传感器芯片（19），其具有多个感光元件。提供参考光源（30）并将光引导到光传感器芯片（19）的至少一部分。此外，提供开环或闭环控制单元（19a），并确定和校正各个感光元件之间的差异。

37. 发明申请

US20060054800A1 Method for investigating transport processes 有权
标题翻译：调查运输过程的方法
公开(公告)号：US20060054800A1
公开(公告)日：2006-03-16
申请号：US11225809
申请日：2005-09-13
申请人： Volker Seyfried
发明人： Volker Seyfried
IPC分类号： H01J3/14 , G01N21/86
CPC分类号： G01N21/6458 , G01N21/6428 , Y10T436/117497
摘要： A method for investigating transport processes in a preferably biological specimen, a laser light beam (2, 3) being guided by means of a scanning apparatus line by line over the specimen within definable specimen regions, and the light proceeding from the specimen being detected by means of a detection apparatus, is characterized, in the interest of the capability of investigating, with high accuracy, processes within the specimen that proceed on a short time scale, in that both an image production light beam (5) for the purpose of observing the specimen and a manipulation light beam (4) for the purpose of manipulating the specimen are used as the laser light beam (2, 3), the image production light beam (5) preceding the manipulation light beam (4) in such a way that pixels of the specimen not yet manipulated by the manipulation light beam (4) are illuminated with the image production light beam (5).
摘要翻译：一种用于研究优选生物样品中的运输过程的方法，通过扫描设备在可限定的样品区域内逐个线上的样品引导的激光束（2,3），并且从样品进行的光由检测装置的特征在于，为了以高精度调查在短时间尺度进行的标本内的处理的能力，为了观察目的，为了观察图像产生光束（5）的能力，将用于操作样本的样本和操作光束（4）用作激光束（2,3），操作光束（4）之前的图像产生光束（5）以这种方式未被操作光束（4）操纵的样本的像素被图像产生光束（5）照亮。

38. 发明授权

US07005654B2 Method for microscopy, and microscope 有权
标题翻译：显微镜方法和显微镜
公开(公告)号：US07005654B2
公开(公告)日：2006-02-28
申请号：US10601804
申请日：2003-06-23
申请人： Volker Seyfried
发明人： Volker Seyfried
IPC分类号： G01N21/64
CPC分类号： G01N21/6458 , G01J3/0229 , G01J2003/1282 , G02B21/0004 , G02B21/0032 , G02B21/0064 , G02B21/0068 , G02B21/008
摘要： The invention discloses a method for microscopy in which a specimen is illuminated with pulsed illuminating light that comprises light from a spectral region, and detection light proceeding from the specimen is detected in a detection spectral region. The method is characterized in that the detection spectral region lies within the spectral region, and that the illuminating light contains no light from the detection spectral region or at least none having the same polarization properties. A microscope is additionally disclosed.
摘要翻译：本发明公开了一种用于显微镜的方法，其中用包括来自光谱区域的光的脉冲照明光照射样本，并且在检测光谱区域中检测从样本进行的检测光。该方法的特征在于检测光谱区域位于光谱区域内，并且照明光不包含来自检测光谱区域的光或至少没有具有相同偏振特性的光。另外公开了显微镜。

39. 发明申请

US20050286122A1 Microscope 审中-公开
标题翻译：显微镜
公开(公告)号：US20050286122A1
公开(公告)日：2005-12-29
申请号：US11167450
申请日：2005-06-27
申请人： Volker Seyfried , Werner Knebel , Jan Schroeder
发明人： Volker Seyfried , Werner Knebel , Jan Schroeder
IPC分类号： G02B21/06 , G02B21/16
CPC分类号： G02B21/16
摘要： A microscope includes at least one illuminating light source and an illumination beam path for guiding the illuminating light to a specimen. A detection beam path guides detected light from the specimen to a detector. An activatable element is positioned in the detection beam path for regulating and/or limiting the light power level in the detection beam path.
摘要翻译：显微镜包括至少一个照明光源和用于将照射光引导到样本的照明光束路径。检测光束路径将检测到的光从样本引导到检测器。可激活元件位于检测光束路径中，用于调节和/或限制检测光束路径中的光功率电平。

40. 发明授权

US07835601B2 Device for generating a laser light beam 有权
标题翻译：用于产生激光束的装置
公开(公告)号：US07835601B2
公开(公告)日：2010-11-16
申请号：US11871389
申请日：2007-10-12
申请人： Volker Seyfried , Rafael Storz
发明人： Volker Seyfried , Rafael Storz
IPC分类号： G02B6/28 , G02B6/36 , H01S3/30 , H01S3/10
CPC分类号： H01S3/02 , G01N21/6458 , G01N2201/024 , G02B21/0032 , H01S3/2383
摘要： A device for generating a laser light beam includes a module. The module includes at least one laser light source, and a mechanical, an electrical and/or an optical interface defined towards an outside of the module.
摘要翻译：用于产生激光束的装置包括模块。该模块包括至少一个激光光源，以及朝向模块的外部限定的机械，电和/或光学接口。

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