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31. 发明专利

DK151396C 未知
公开(公告)号：DK151396C
公开(公告)日：1988-07-04
申请号：DK172374
申请日：1974-03-28
申请人： BOEHRINGER MANNHEIM GMBH
发明人： BEAUCAMP KLAUS , MOLLERING HANS , LANG GUNTER , GRUBER WOLFGANG , ROESCHLAU PETER
IPC分类号： C12N9/18 , C12Q1/60 , C12Q1/00
摘要： 1429526 Cholesterol esterase BOEHRINGER MANNHEIM GmbH 25 March 1974 [28 March 1973 3 April 1973] 13159/74 Heading C3H [Also in Division G1] Cholesterol esterase-containing materials are obtained from Candida rugosa ATCC 14830, Aspergillus spec. WS 90030 and the following micro-organisms: Actinomyces aureoverticillium WS 90002, Actinomyces cyaneofuscatus WS 90003, Actinomyces griseomycini WS 90004, Actinomyces longisporus-fl. WS 90005, Actinomyces malachiticus WS 90006, Actinomyces roseolus WS 90007, Actinomyces toxytricini WS 90008, Actinomyces variabilis Streptomyces spec. WS 90010, Streptomyces autotrophicus WS 90011, Streptomyces canescens WS 90012, Streptomyces chartreusis WS 90013, Streptomyces michiganensis WS 90014, Streptomyces murinus WS 90015, Streptomyces hachijoensis WS 90016, Streptomyces caelestes WS 90017, Streptomyces tendae WS 90018, Nocardia rubra WS 90019, Candida mycoderma WS 90020, Candida albicans WS 90021, Candida albicans WS 90022, Candida albicans WS 90023, Candida spec. WS 90024, Cunninghamella elegans WS 90025, Mucor mucedo WS 90026, Shizopus spec. WS 90027, Penicillium spec. WS 90028 and Aspergillus spec. WS 90029. The micro-organisms may be used as such or in digested form, e.g. as an acetonedried powder as sources of cholesterol esterase. The micro-organisms are preferably cultured in nutrient media containing cholesterol esters, multistage culturing being particularly suitable.

32. 发明专利

DK149759C 未知
公开(公告)号：DK149759C
公开(公告)日：1987-03-02
申请号：DK41772
申请日：1972-01-31
申请人： BOEHRINGER MANNHEIM GMBH
发明人： WAHLEFELD AUGUST WILHELM , BERNT ERICH , GRUBER WOLFGANG , BERGMEYER HANS ULRICH
IPC分类号： G01N33/72 , G01N33/50
摘要： 1330094 Test pack for bilirubin BOEHRINGER MANNHEIM GmbH 2 March 1972 [5 March 1971] 9794/72 Heading B1X A test pack for the determination of total bilirubin comprises, 2,4-dichtoro-, 2,5-dichloro- or 2-chloro-4-nitro-phenyl diazonium chloride and an alkyl-phenol polyethylene oxide. The diazonium compound may be the solid salt; the polyethylene oxide may be dissolved in HCl, H 2 SO 4 or H 3 PO 4 . Alternatively the components and the acid may be mixed, but the storage stability is thereby reduced. The composition may also contain a stabiliser for the diazonium salt. In use, a serum sample is added to a known volume of reagent and the extinction measured at 546 nm when the end valve is reached.

33. 发明专利

DK148362C 未知
公开(公告)号：DK148362C
公开(公告)日：1985-11-04
申请号：DK319180
申请日：1980-07-24
申请人： BOEHRINGER MANNHEIM GMBH
发明人： BEAUCAMP KLAUS , NELBOECK MICHAEL , GAUHL HELMGARD , SEIDEL HANS , GRUBER WOLFGANG , BRUNNER HERWIG
IPC分类号： C12N1/14 , C12N1/20 , C12N9/18 , C12Q1/60 , C12R1/38
摘要： The present invention provides a process for obtaining cholesterol esterase from micro-organisms, wherein a micro-organism capable of cholesterol esterase formation is cultured in an appropriate nutrient medium in the presence of lecithin as inducer and the enzyme is obtained from the culture medium and/or from the cells.

34. 发明专利

SU1168103A3 METHOD OF DETERMINING TOTAL CHOLESTEROL IN BLOOD SERUM 未知
公开(公告)号：SU1168103A3
公开(公告)日：1985-07-15
申请号：SU2012029
申请日：1974-03-28
申请人： BOEHRINGER MANNHEIM GMBH
发明人： BEAUCAMP KLAUS , MOELLERING HANS , LANG GUENTER , GRUBER WOLFGANG , ROESCHLAU PETER
IPC分类号： C12Q1/60 , A61B10/00 , C12R1/01 , C12R1/365 , C12R1/38 , C12R1/845 , G01N20060101 , G01N33/15 , G01N33/48

35. 发明专利

CS236751B2 PROCESSING OF SUBSTRATES FOR DETERMINING OF ALPHA-AMYLASE 未知
公开(公告)号：CS236751B2
公开(公告)日：1985-05-15
申请号：CS824079
申请日：1978-09-13
申请人： BOEHRINGER MANNHEIM GMBH
发明人： RAUSCHER ELLI , NEUMANN ULRICH , WAHLEFELD AUGUST W , HAGEN ALEXANDER , GRUBER WOLFGANG , ZIEGENHORN JOACHIM , SCHAICH EUGEN , DENEKE ULFERT , MICHAL GERHARD , WEIMANN GUENTER
IPC分类号： C08B37/00 , C12Q1/40 , G01N33/48 , G01N31/00

36. 发明专利

ZA8406093B HEPTAOSE COMPOUNDS,PREPARATION AND USE THEREOF 未知
公开(公告)号：ZA8406093B
公开(公告)日：1985-04-24
申请号：ZA8406093
申请日：1984-08-07
申请人： BOEHRINGER MANNHEIM GMBH
发明人： RAUSCHER ELLI , SCHAICH EUGEN , NEUMANN ULRICH , WAHLEFELD AUGUST , GRUBER WOLFGANG , EMPL BERNHARD
IPC分类号： G01N33/50 , C07H3/06 , C07H15/04 , C07H15/203 , C07H21/00 , C08B37/00 , C12Q1/40 , A61K , C07H , C12Q
CPC分类号： C07H21/00 , C07H3/06 , C12Q1/40 , C12Q2334/10 , Y10S435/81

37. 发明专利

CA1174149A PROCESS FOR THE ENZYMATIC DETERMINATION OF FREE AND ESTERIFIED CHLORESTEROL 未知
公开(公告)号：CA1174149A
公开(公告)日：1984-09-11
申请号：CA171416
申请日：1973-05-15
申请人： BOEHRINGER MANNHEIM GMBH
发明人： GRUBER WOLFGANG , BERGMEYER HANS U , BERNT ERICH , HAGEN ALEXANDER , ROESCHLAU PETER , LANG GUNTER , BEAUCAMP KLAUS
IPC分类号： C12Q1/60 , G01N33/92
摘要： Cholesterol is determined by incubating a sample suspectod of containing cholesterol in an aqueous medium with cholesterol oxidase and determining either the oxygen consumption, the hydrogen peroxide formed, or cholestenone, as a measure of the initial cholesterol content; reagent compositions comprising choloaterol oxidase and a system for the determination of H2O2 or for the determination of cholestenone are provided.

38. 发明专利

DK147163B 未知
公开(公告)号：DK147163B
公开(公告)日：1984-04-30
申请号：DK303172
申请日：1972-06-16
申请人： BOEHRINGER MANNHEIM GMBH
发明人： HAGEN ALEXANDER , BERGMEYER HANS ULRICH , GRUBER WOLFGANG , BEAUCAMP KLAUS , JAWOREK DIETER
IPC分类号： G01N27/48 , B01L99/00 , C12Q1/00 , G01N33/487
摘要： 1392878 Delivering measured quantities of liquid BOEHRINGER MANNHEIM GmbH 16 June 1972 [18 June 1971] 28229/72 Heading B8N [Also in Division G1] A device for introduction of a predetermined amount of air-bubble-free sample solution into a flowing stream of liquid without altering the velocity of flow comprises casing 10 having inlet bores 13a, 12a for the flowing stream from a common feed line (12, Fig. 4, not shown), bore 21 for introduction of sample and bore 20 for introduction of air, and outlet bores 15a, 14a for the flowing stream and 16a for application of vacuum, and core 11 longitudinally slidable in casing 10 and having bores 17, 18, 19, the arrangement of the bores being such that in use of the device with the core as shown and a stream of liquid flowing through 13a, 17, 15a, sample is placed in opening 21 by pouring it in, by dipping the bore opening into sample or by opening it to a container of sample, then the core is moved to connect 12a, 18, 14a when the sample is carried out of bores 18, 14a by the flow of liquid and simultaneously any sample remaining in 21 is sucked away through 19, 16a, the core is moved to connect 20, 18, 16a when liquid remaining in bore 18 is removed and the liquid stream can again flow in 13a, 17, 15a, then the core is returned to its original position which is as shown.

39. 发明专利

HU183202B PROCESS FOR PREPARING CHOLESTEROL ESTERASE 未知
公开(公告)号：HU183202B
公开(公告)日：1984-04-28
申请号：HU206680
申请日：1980-08-19
申请人： BOEHRINGER MANNHEIM GMBH
发明人： BEUCAMP KLAUS , NELBOECK MICHAEL , GAUHL HELMGARD , SEIDEL HANS , GRUBER WOLFGANG , BRUNNER HERWIG
IPC分类号： C12N9/18 , C12Q1/60 , C12R1/38
摘要： The present invention provides a process for obtaining cholesterol esterase from micro-organisms of the genus Pseudomonas, wherein Pseudomonas sp. DSM 1280 or DSM 1281 is cultured in an appropriate culture medium in the presence of an inducer and the enzyme is obtained from the culture medium and/or from the cells.

40. 发明专利

DK146399C 未知
公开(公告)号：DK146399C
公开(公告)日：1984-03-05
申请号：DK303272
申请日：1972-06-16
申请人： BOEHRINGER MANNHEIM GMBH
发明人： HAGEN ALEXANDER , GRUBER WOLFGANG , BERGMEYER HANS ULRICH , JAWOREK DIETER
IPC分类号： G01N27/48 , C12Q1/00 , G01N33/487 , C12Q1/26

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