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    • 31. 发明申请
    • NOVEL METHOD OF ASSAYING NUCLEIC ACID USING LABELED NUCLEOTIDE
    • 使用标记核苷酸测定核酸的新方法
    • US20090233308A1
    • 2009-09-17
    • US12421983
    • 2009-04-10
    • Shinya KURATAKyoko TakatsuKazunori NakamuraTakahiro Kanagawa
    • Shinya KURATAKyoko TakatsuKazunori NakamuraTakahiro Kanagawa
    • C12Q1/68
    • C12Q1/6844C12Q2563/107
    • A novel method is provided to assay at least one nucleic acid. According to this method, a nucleic acid polymerization reaction is conducted in a nucleic acid polymerization reaction system, which contains (A) the at least one nucleic acid as a template, (B) at least one nucleotide monomer labeled with at least one label selected from the group consisting of (a) fluorescent dyes, (b) quenchers and (c) immune related substances with a fluorescent dye or quencher contained therein, and (C) at least one nucleic acid-synthesizing enzyme. The template nucleic acid or a nucleic acid, which has been synthesized using the template nucleic acid as a template, is then assayed from a change or an amount of a change in an optical character of the nucleic acid polymerization system. This method makes it possible to specifically and accurately assay at least one nucleic acid, which is contained in a single system and can be an unknown nucleic acid and/or a known nucleic acid, with excellent sensitivity, in short time and with ease.
    • 提供了一种新的方法来测定至少一种核酸。 根据该方法,在核酸聚合反应体系中进行核酸聚合反应,所述核酸聚合反应体系含有(A)至少一种核酸作为模板,(B)至少一种用至少一个选自标记的标记的核苷酸单体 由(a)荧光染料,(b)猝灭剂和(c)与其中含有荧光染料或猝灭剂的免疫相关物质组成的组,和(C)至少一种核酸合成酶。 然后使用模板核酸作为模板合成的模板核酸或核酸从核酸聚合体系的光学特性的变化或量的变化来测定。 该方法使得可以在短时间和容易地特异性且准确地测定包含在单一系统中并且可以是未知核酸和/或已知核酸的至少一种核酸,具有优异的灵敏度。
    • 33. 发明授权
    • Hydraulic drive device
    • 液压驱动装置
    • US07434394B2
    • 2008-10-14
    • US10553414
    • 2004-04-16
    • Kouji IshikawaYusuke KajitaKazunori NakamuraGenroku SugiyamaHideo Karasawa
    • Kouji IshikawaYusuke KajitaKazunori NakamuraGenroku SugiyamaHideo Karasawa
    • F16D31/02F15B13/06
    • E02F9/2235E02F9/2285E02F9/2296
    • To permit using hold-side pressure oil in a first hydraulic cylinder for the acceleration of a second hydraulic cylinder upon performing a combined operation of the first and second hydraulic cylinders, a hydraulic drive system is provided with a main hydraulic pump 21, a boom cylinder 6 and an arm cylinder 7, a directional control valve 23 for a boom and a directional control valve 24 for an arm, and a boom control device 25 and an arm control device 26. The hydraulic drive system is also provided with a pressure oil feed means for feeding pressure oil in a rod chamber 6b of the boom cylinder 6 to an upstream side of the directional control valve 24 for the arm when a bottom pressure of the arm cylinder 7 has increased to a high pressure equal to or higher than a predetermined pressure. This pressure oil feed means includes a flow combiner valve 44 arranged in a reservoir line 42 which can communicate to the rod chamber 6b of the boom cylinder 6. When the bottom pressure of the arm cylinder 7 is the predetermined pressure or higher, the flow combiner valve 44 maintains a communication line 40, which communicates the reservoir line 42 and the upstream side of the directional control valve 24 for the arm with each other, in a state that pressure oil can be fed to the upstream side of the directional control valve 24 for the arm.
    • 为了在执行第一和第二液压缸的组合操作时允许在第一液压缸中使用保持侧压力油来加速第二液压缸,液压驱动系统设置有主液压泵21,动臂缸 悬臂气缸7,用于悬臂的方向控制阀23和用于臂的方向控制阀24,以及起重臂控制装置25和臂控制装置26。 液压驱动系统还设置有压力油进给装置,用于当臂缸7的底部压力向臂的方向控制阀24的上游侧供给动臂缸6的杆室6b中的压力油时 已经增加到等于或高于预定压力的高压。 该压力供油装置包括设置在储存管路42中的流动组合器阀44,该储存管路42可与动臂缸6的杆室6b连通。 当气缸7的底部压力为预定压力或更高压力时,组合阀44保持连通管路40,该连通管路40将储存管路42和臂的方向控制阀24的上游侧彼此连通, 在压力油可以供给到用于臂的方向控制阀24的上游侧的状态下。
    • 37. 发明申请
    • Hydraulic drive device
    • 液压驱动装置
    • US20070028607A1
    • 2007-02-08
    • US10553414
    • 2004-04-16
    • Kouji IshikawaYusuke KajitaKazunori NakamuraGenroku SugiyamaHideo Karasawa
    • Kouji IshikawaYusuke KajitaKazunori NakamuraGenroku SugiyamaHideo Karasawa
    • F03C1/00
    • E02F9/2235E02F9/2285E02F9/2296
    • To permit using hold-side pressure oil in a first hydraulic cylinder for the acceleration of a second hydraulic cylinder upon performing a combined operation of the first and second hydraulic cylinders, a hydraulic drive system is provided with a main hydraulic pump 21, a boom cylinder 6 and an arm cylinder 7, a directional control valve 23 for a boom and a directional control valve 24 for an arm, and a boom control device 25 and an arm control device 26. The hydraulic drive system is also provided with a pressure oil feed means for feeding pressure oil in a rod chamber 6b of the boom cylinder 6 to an upstream side of the directional control valve 24 for the arm when a bottom pressure of the arm cylinder 7 has increased to a high pressure equal to or higher than a predetermined pressure. This pressure oil feed means includes a flow combiner valve 44 arranged in a reservoir line 42 which can communicate to the rod chamber 6b of the boom cylinder 6. When the bottom pressure of the arm cylinder 7 is the predetermined pressure or higher, the flow combiner valve 44 maintains a communication line 40, which communicates the reservoir line 42 and the upstream side of the directional control valve 24 for the arm with each other, in a state that pressure oil can be fed to the upstream side of the directional control valve 24 for the arm.
    • 为了在执行第一和第二液压缸的组合操作时允许在第一液压缸中使用保持侧压力油来加速第二液压缸,液压驱动系统设置有主液压泵21,动臂缸 悬臂气缸7,用于悬臂的方向控制阀23和用于臂的方向控制阀24,以及悬臂控制装置25和臂控制装置26.液压驱动系统还设有压力油进给 用于当臂缸7的底部压力增加到等于或高于其的高压时将臂架缸6的杆室6b中的压力油供给到用于臂的方向控制阀24的上游侧的装置 预定压力。 该压力供油装置包括配置在储存管路42中的流动组合器阀44,其可与臂缸6的杆室6b连通。当臂缸7的底部压力为预定压力或更高时, 组合阀44保持连通管线40,该连通管线40在压力油可以被供给到方向控制阀的上游侧的状态下将储存管路42和用于臂的方向控制阀24的上游侧彼此连通 24为手臂。