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    • 34. 发明申请
    • Recombinant Microorganism
    • 重组微生物
    • US20110151567A1
    • 2011-06-23
    • US12530135
    • 2008-04-08
    • Shenghao LiuKeiji EndoKatsutoshi Ara
    • Shenghao LiuKeiji EndoKatsutoshi Ara
    • C12N15/87C12N1/21C12N9/50C12N9/42
    • C12P21/02
    • A recombinant microorganism having improved productivity for a protein or a polypeptide, and a method for producing a protein or a polypeptide using the recombinant microorganism, are provided. A recombinant microorganism obtained by transfecting a gene for encoding a desired protein or polypeptide into a microorganism strain which is obtained by genetically constructing to overexpress secY gene of Bacillus subtilis or a gene corresponding to the secY gene, and deleting or inactivating one or more genes selected from sporulation-associated genes and genes corresponding to the sporulation-associated genes from the genome.
    • 提供了具有提高的蛋白质或多肽的生产力的重组微生物,以及使用该重组微生物生产蛋白质或多肽的方法。 将通过将所需蛋白质或多肽编码的基因转染到通过遗传构建以过表达枯草芽孢杆菌的secY基因或相应于所述secY基因的基因而获得的微生物菌株获得的重组微生物,以及删除或灭活所选择的一种或多种基因 来自与来自基因组的孢子形成相关基因相对应的孢子形成相关基因和基因。
    • 35. 发明申请
    • USE OF SULFONANILIDES AS HERBICIDE
    • 使用磺酰亚胺作为除草剂
    • US20100323896A1
    • 2010-12-23
    • US12868032
    • 2010-08-25
    • Koichi ArakiYoshitaka SatoTakuya GomibuchiKeiji EndoShinichi ShirakuraShin NakamuraChristopher RosingerDieter Feucht
    • Koichi ArakiYoshitaka SatoTakuya GomibuchiKeiji EndoShinichi ShirakuraShin NakamuraChristopher RosingerDieter Feucht
    • A01N43/66C07D239/52C07D251/16A01N43/54A01P13/00
    • C07D251/20A01N43/54A01N43/66C07D239/52
    • Herbicidal compositions comprising containing sulfonanilides of the formula (I) wherein R1 represents hydrogen, fluorine, chlorine, C1-4 alkyl, C1-4 alkoxy, C3-6 cycloalkyl-C1-4 alkyloxy or C1-4 haloalkoxy, R2 represents hydrogen, fluorine or chlorine, R3 represents hydrogen or fluorine, R4 represents hydrogen or C1-4 alkyl which may be optionally C1-4 alkoxy-substituted, C3-6 alkenyl or C3-6 alkynyl, R5 represents hydrogen, R6 represents hydroxy, fluorine or chlorine, or R5 and R6 may form, together with the carbon to which they are bonded, C═O, and X represents CH or N, provided that the following cases are excluded: (i) R1 represents hydrogen, fluorine or chlorine, R2 represents hydrogen, R3 represents hydrogen, R4 represents hydrogen, R5 represents hydrogen, and R6 represents hydroxy, (ii) R1 represents hydrogen, fluorine or chlorine, R2 represents hydrogen, R3 represents hydrogen, R4 represents hydrogen, and R5 and R6 form C═O together with the carbon to which they are bonded, (iii) R1 represents C1-4 alkyl, R2 represents hydrogen, R3 represents hydrogen, R4 represents hydrogen, R5 represents hydrogen, R6 represents hydroxy, and X represents CH, or (iv) R1 represents C1-4 alkyl, R2 represents hydrogen, R3 represents hydrogen, R4 represents hydrogen, R5 and R6 form C═O together with the carbon to which they are bonded, and X represents CH, and new compounds being embraced by the formula (I).
    • 包含含有式(I)的磺酰苯胺的除草组合物,其中R 1表示氢,氟,氯,C 1-4烷基,C 1-4烷氧基,C 3-6环烷基-C 1-4烷氧基或C 1-4卤代烷氧基,R 2表示氢,氟 或氯,R 3表示氢或氟,R 4表示氢或可以任选被C 1-4烷氧基取代的C 1-4烷基,C 3-6烯基或C 3-6炔基,R 5表示氢,R 6表示羟基,氟或氯, 或者R 5和R 6可以与它们所键合的碳一起形成C = O,X表示CH或N,条件是排除以下情况:(i)R 1表示氢,氟或氯,R 2表示氢 ,R3表示氢,R4表示氢,R5表示氢,R6表示羟基,(ii)R1表示氢,氟或氯,R2表示氢,R3表示氢,R4表示氢,R5和R6表示C = O 与它们结合的碳, (iii)R 1表示C 1-4烷基,R 2表示氢,R 3表示氢,R 4表示氢,R 5表示氢,R 6表示羟基,X表示CH,或(ⅳ)R 1表示C 1-4烷基, R3表示氢,R4表示氢,R5和R6与它们所键合的碳一起形成C = O,X表示CH,并且新化合物被式(I)所包围。
    • 36. 发明授权
    • Herbicide composition for paddy field
    • 稻田除草剂组成
    • US07855165B2
    • 2010-12-21
    • US11622514
    • 2007-01-12
    • Keiji EndoShinichi ShirakuraShin NakamuraNatsuko Minegishi
    • Keiji EndoShinichi ShirakuraShin NakamuraNatsuko Minegishi
    • A01N43/64A01N43/60
    • A01N43/54A01N43/66A01N25/32A01N37/18A01N37/22A01N39/02A01N43/08A01N43/12A01N43/56A01N43/78A01N43/80A01N43/86A01N43/90A01N47/30A01N47/36A01N47/38A01N57/14A01N2300/00
    • A herbicide composition for paddy field characterized by containing (a) a herbicidal difluoromethanesulfonamide derivative represented by the formula (I) and (b) at least one herbicidal compound selected from the group consisting of pretilachlor, butachlor, alachlor, metolachlor, acetochlor, clomeprop, bromobutide, benfuresate, indanofan, pyrazolate, benzofenap, pyrazoxyfen, pyraclonil, oxaziclomefone, bensulfuron-methyl, azimsulfuron, imazosulfuron, pyrazosulfuron-ethyl, cyclosulfamuron, ethoxysulfuron, halosulfuron-methyl, orthosulfamuron, cinosulfuron, metsulfuron-methyl, penoxsulam, thiobencarb, pyributycarb, molinate, dimethametryn, simetryn, cafenstrole, quinclorac, anilofos, mefenacet, fentrazamide, pentoxazone, oxadiargyl, oxadiazon, benzobicyclon, mesotrione, AVH301 (code no.), cyhalofop-butyl, metamifop, bispyribac-sodium, pyriftalid, pyrimisulfan, pyrimenobac-methyl, chlormethoxynil, oxyfluorfen, dithiopyr, MCPA, MCPB, 2,4-D, dymron, cumyluron, quinoclamine and clomazone, and/or (c) one or more compounds selected from the group consisting of safeners dymron, isoxadifen(-ethyl), flurazole, fenchlorazole-ethyl, fenclorim, cloquintocet-mexyl, oxabetrinil, fluxofenim, mefenpyr-diethyl, furilazole, R-29148 (code no.), benoxacor, dichlormid and dicyclonone as effective components.
    • 一种用于稻田的除草剂组合物,其特征在于含有(a)由式(I)和(b)表示的除草二氟甲磺酰胺衍生物,至少一种选自草酰草胺,丁草胺,甲草胺,异丙甲草胺,乙草胺,氯米普, 溴替丁,苯呋酮,吡唑啉,吡嘧磺隆,苯唑芬酮,吡嘧磺隆,吡嘧磺隆,恶唑磺隆,吡嘧磺隆,吡嘧磺隆,咪唑磺隆,吡嘧磺隆,环磺胺嘧啶,乙氧基磺隆,卤代磺隆,邻氨基磺隆, 茉莉酸,二甲基苯甲酸,西妥昔单抗,喹诺酮,芬太拉唑,依诺沙星,恶草精,恶二酮,苯并环己酮,甲基磺草酮,AVH301(编号),cyhalofop-butyl,metamifop,bispyribac-sodium,pyriftalid, ,氯氟乙氧基,氧氟芬,二硫代吡啶,MCPA,MCPB,2,4-D,胞嘧啶,异戊肾上腺素,喹诺酮和克罗莫他, d /或(c)一种或多种选自安息剂dymron,异恶草酮( - 乙基),氟尿嘧啶,芬氯唑,芬舍洛芬,氯喹毒素,奥沙利坦,氟吡芬,咪芬吡啶二乙基,呋喃唑,R-29148 (代号),benoxacor,dichlormid和dicyclonone作为有效成分。
    • 38. 发明申请
    • Recombinant Microorganism
    • 重组微生物
    • US20100255534A1
    • 2010-10-07
    • US12528039
    • 2007-12-27
    • Keiji EndoKatsutoshi Ara
    • Keiji EndoKatsutoshi Ara
    • C12P21/00C12N1/21
    • C12N15/75C07K14/32C12P21/02
    • Provision of a recombinant microorganism which has increased productivity of a protein or polypeptide of interest and a method for producing a protein or polypeptide of interest using the recombinant microorganism. The recombinant microorganism is produced by transferring a gene encoding a protein or polypeptide of interest to a microorganism strain, wherein the microorganism strain is prepared by: introducing a transcription initiation regulatory region that functions in the microorganism or both the transcription initiation regulatory region and a ribosome-binding site that functions in the microorganism into the upstream of a Bacillus subtilis prsA gene or a gene corresponding thereto in the genome of a parental microorganism, or introducing a gene fragment prepared by ligating a transcription initiation regulatory region that functions in the microorganism or both the transcription initiation regulatory region and a ribosome-binding site that functions in the microorganism to the upstream of the Bacillus subtilis prsA gene or a gene corresponding thereto into the genome of a parental microorganism; and deleting or inactivating one or more genes selected from an abrB gene, a dltA gene, a dltB gene, a dltC gene, a dltD gene, a dltE gene, and a gene (genes) corresponding thereto.
    • 提供具有增加的目的蛋白质或多肽的生产力的重组微生物以及使用重组微生物产生感兴趣的蛋白质或多肽的方法。 通过将编码目的蛋白质或多肽的基因转移到微生物菌株来产生重组微生物,其中微生物菌株通过以下方式制备:引入在微生物中起作用的转录起始调节区或转录起始调节区和核糖体 在微生物中起作用的枯草芽孢杆菌prsA基因或与亲本微生物基因组相对应的基因的上游的引入位点,或引入通过连接在微生物中起作用的转录起始调节区或两者兼有的基因片段 转录起始调节区和在微生物中起作用的枯草芽孢杆菌prsA基因上游的核糖体结合位点或与之相对应的基因转化为亲本微生物的基因组; 以及删除或失活一个或多个选自abrB基因,dltA基因,dltB基因,dltC基因,dltD基因,dltE基因和与其相对应的基因(基因)的基因。
    • 39. 发明申请
    • Mutant bacterium belonging to the genus bacillus
    • 属于杆菌属的突变细菌
    • US20090170154A1
    • 2009-07-02
    • US10590275
    • 2005-03-04
    • Keiji EndoKatsuya Ozaki
    • Keiji EndoKatsuya Ozaki
    • C12P21/00C12N1/21C12N15/87
    • C12N9/2417C12N9/2437C12N9/54C12Y302/01004
    • The present invention provides a mutant Bacillus bacterium capable of enhancing productivity of proteins or polypeptides, a recombinant microorganism produced by introducing genes encoding heterologous proteins or polypeptides into the mutant Bacillus bacterium, and a method for producing proteins or polypeptides by use of the recombinant microorganism.A mutant Bacillus bacterium which has, on the genome or plasmid thereof, DNA having a promoter sequence which is recognized and transcribed specifically during the sporulation stage and which is ligated to an upstream end of a sigA gene or a gene equivalent thereto, a recombinant microorganism produced by introducing genes encoding heterologous proteins or polypeptides into the mutant Bacillus bacterium, and a method for producing proteins or polypeptides by use of the recombinant microorganism.
    • 本发明提供能够提高蛋白质或多肽生产力的突变芽孢杆菌属细菌,通过将编码异源蛋白质或多肽的基因导入突变芽孢杆菌属细菌产生的重组微生物,以及通过使用重组微生物产生蛋白质或多肽的方法。 一种突变芽孢杆菌属细菌,其在基因组或质粒上具有在孢子形成阶段中特异性识别并转录并与sigA基因或其等同基因相连的基因的上游端的启动子序列的DNA,重组微生物 通过将编码异源蛋白质或多肽的基因导入突变芽孢杆菌属细菌而产生,以及通过使用重组微生物产生蛋白质或多肽的方法。