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    • 33. 发明公开
    • Process for selecting and maintaining recombinant DNA in lactic acid bacteria
    • Verfahren zur Selektion und Erhohlung rekombinanter DNA inMilchsäurebakterien。
    • EP0355036A1
    • 1990-02-21
    • EP89201454.9
    • 1989-06-06
    • NEDERLANDS INSTITUUT VOOR ZUIVELONDERZOEK
    • De Vos, Willem Meindert
    • C12N15/00C12N1/20
    • C12N9/2402C12N15/52C12N15/68C12N15/746C12Y302/01085
    • Procedure for selecting lactic acid bacteria which contain recombinant DNA, and keeping said DNA stable, with the aid of a marker acceptable in foodstuffs for human consumption, which procedure is characterized in that a lactic acid bacterium which lacks one or two enzymes with sufficient activity and indispensable for lactose fermentation is transformed with a DNA fragment which, as a marker, codes at least for the enzyme or enzymes missing in said lactic acid bacterium and indispensable for lactose fermentation. Said DNA-fragment may also code for a function which imparts an improved or new characteristic to the lactic acid bacterium, for instance for the production of proteolytic enzymes, for insensitivity to bacteriophages, for bacteriocin production and/or bacteriocin immunity or for β-­galactosidase, α-amylase, chymosin or pepsin.
    • 选择含有重组DNA的乳酸菌,并借助于食品中可接受的用于人类消费的标记物来保持所述DNA稳定的方法,其特征在于,缺少一种或两种具有足够活性的酶的乳酸菌和 用作为标记的DNA片段转化乳糖发酵必不可少的DNA片段,至少对所述乳酸菌缺失的酶或酶进行编码,并且对乳糖发酵是不可缺少的。 所述DNA片段还可以编码赋予乳酸菌的改进或新特征的功能,例如用于产生蛋白水解酶,对噬菌体不敏感,对细菌素生成和/或细菌素免疫或用于β-半乳糖苷酶 ,α-淀粉酶,凝乳酶或胃蛋白酶。