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21. 发明申请

US20150211076A1 POLYNUCLEOTIDE PRIMERS 审中-公开
公开(公告)号：US20150211076A1
公开(公告)日：2015-07-30
申请号：US14682620
申请日：2015-04-09
申请人： QIAGEN MANCHESTER LIMITED
发明人： David Mark WHITCOMBE , Nicola Jo THELWELL , Paul Francis RAVETTO
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q1/6851 , C12Q1/6883 , C12Q2600/156 , C12Q2600/16 , C12Q2537/155
摘要： A polynucleotide primer comprising at least the final six nucleotides of one of the following primer sequences, or a sequence complementary thereto: SEQ. ID NOS. 1 to 18, 21 to 45 or 74 to 77.
摘要翻译：至少包含以下引物序列之一的最后六个核苷酸或其互补序列的多核苷酸引物：SEQ ID NO： ID NOS。 1至18，21至45或74至77。

22. 发明授权

US09029084B2 Polynucleotide primers 有权
标题翻译：多核苷酸引物
公开(公告)号：US09029084B2
公开(公告)日：2015-05-12
申请号：US11910511
申请日：2006-04-04
申请人： David Mark Whitcombe , Nicola Jo Thelwell , Paul Francis Ravetto
发明人： David Mark Whitcombe , Nicola Jo Thelwell , Paul Francis Ravetto
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q1/6851 , C12Q1/6883 , C12Q2600/156 , C12Q2600/16 , C12Q2537/155
摘要： A polynucleotide primer comprising at least the final six nucleotides of one of the following primer sequences, or a sequence complementary thereto: SEQ. ID NOS. 1 to 18, 21 to 45 or 74 to 77.
摘要翻译：至少包含以下引物序列之一的最后六个核苷酸或其互补序列的多核苷酸引物：SEQ ID NO： ID NOS。 1至18，21至45或74至77。

23. 发明申请

US20140031238A1 ALTERNATIVE NUCLEOTIDE FLOWS IN SEQUENCING-BY-SYNTHESIS METHODS 审中-公开
标题翻译：序列合成方法中的替代核酸流
公开(公告)号：US20140031238A1
公开(公告)日：2014-01-30
申请号：US13859667
申请日：2013-04-09
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： Jonathan SCHULTZ , John DAVIDSON
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , B01L3/5027 , C12Q1/6874 , C12Q2537/155 , C12Q2563/149 , C12Q2565/301 , C12Q2565/629
摘要： A method for sequencing a polynucleotide strand by using sequencing-by-synthesis techniques. To address the problem of incomplete extension (IE) and/or carry forward (CF) errors that can occur in sequencing-by-synthesis reactions, an alternative flow ordering of dNTPs is used. In contrast to conventional flow orderings, the dNTPs are flowed in an ordering that is not a continuous repeat of an ordering of the four different dNTPs. This alternate flow ordering may reduce the loss of phasic synchrony in the population of template polynucleotide strands that result from IE and/or CF errors.
摘要翻译：通过使用按顺序合成技术测序多核苷酸链的方法。为了解决在逐个合成反应中可能发生的不完全扩展（IE）和/或结转（CF）错误的问题，使用了dNTP的替代流顺序。与常规流顺序相反，dNTP以不是四种不同dNTP的顺序的连续重复的顺序流动。这种交替流顺序可以减少由IE和/或CF错误导致的模板多核苷酸链群体中的相位同步损失。

24. 发明申请

US20040197774A1 Representational approach to DNA analysis 审中-公开
标题翻译：代表性DNA分析方法
公开(公告)号：US20040197774A1
公开(公告)日：2004-10-07
申请号：US09935367
申请日：2001-08-22
发明人： Michael Wigler , Nikolai Lisitsyn
IPC分类号： C12Q001/68
CPC分类号： C12Q1/6853 , C12Q1/6809 , C12Q1/683 , C12Q1/6855 , C12Q2539/107 , C12Q2525/191 , C12Q2531/113 , C12Q2537/155
摘要： Methodology is provided for developing probes for identifying sequence differences between two related DNA populations, sets of DNA fragments or collections of restriction-endonuclease-cleaved DNA or cDNA. The method employs an initial stage to obtain a representation of both DNA populations, namely using the PCR to produce relatively short fragments, referred to as amplicons. Tester amplicons containing target DNA, sequences of interest, are ligated to adaptors and mixed with excess driver amplicons under melting and annealing conditions, followed by PCR amplification. The process may be repeated so as to greatly enrich the target DNA. Optionally, the target DNA may then be cloned and the DNA used as probes.
摘要翻译：提供了用于开发用于鉴定两个相关DNA群体，DNA片段集合或限制性内切核酸酶切割的DNA或cDNA集合之间的序列差异的探针的方法。该方法采用初始阶段来获得两个DNA群体的表示，即使用PCR产生相对较短的片段，称为扩增子。将目标DNA（目的序列）的测试仪扩增子连接到衔接子上，并在熔融和退火条件下与过量的驱动子扩增子混合，然后进行PCR扩增。可以重复该过程以大大丰富靶DNA。任选地，可以克隆目标DNA，并将DNA用作探针。

25. 发明申请

US20030087279A1 Analysis of gene expression profiles using sequential hybridization 有权
标题翻译：使用顺序杂交分析基因表达谱
公开(公告)号：US20030087279A1
公开(公告)日：2003-05-08
申请号：US10222459
申请日：2002-08-16
发明人： Zhifeng Shao , Sitong Sheng , Shoudan Liang
IPC分类号： C12Q001/68
CPC分类号： C12Q1/6876 , C12Q1/6809 , C12Q2600/158 , C12Q2565/519 , C12Q2537/155 , C12Q2537/149
摘要： This invention relates generally to analysis of gene expression profiles. In particular, the present invention provides a method for a method for analyzing gene expression profiles of a cell, which method comprises: a) providing for isolated mRNA or cDNA target sequences from a cell; b) sequentially hybridizing said isolated mRNA or cDNA target sequences with a plurality of nucleotide probes; and c) assessing the sequential hybridization between said isolated mRNA or cDNA target sequences and said plurality of nucleotide probes to analyze gene expression profiles of said cell. Systems for analyzing gene expression profiles are also provided. Optical devices for detecting hybridization signal are further provided.
摘要翻译：本发明一般涉及基因表达谱的分析。特别地，本发明提供了一种分析细胞基因表达谱的方法，该方法包括：a）从细胞提供分离的mRNA或cDNA靶序列; b）将所述分离的mRNA或cDNA靶序列与多个核苷酸探针顺序杂交; 和c）评估所述分离的mRNA或cDNA靶序列与所述多个核苷酸探针之间的顺序杂交，以分析所述细胞的基因表达谱。还提供了分析基因表达谱的系统。还提供用于检测杂交信号的光学装置。

26. 发明公开

US20230348969A1 METHODS AND SYSTEMS FOR NUCLEIC ACID SEQUENCING 审中-公开
公开(公告)号：US20230348969A1
公开(公告)日：2023-11-02
申请号：US18132703
申请日：2023-04-10
申请人： ULTIMA GENOMICS, INC.
发明人： Mark PRATT , Florian OBERSTRASS , Linda LEE , Steven MENCHEN
IPC分类号： C12Q1/6874
CPC分类号： C12Q1/6874 , C12Y207/07007 , C12Q2537/157 , C12Q2549/10 , C12Q2537/155 , C12Q2535/113
摘要： The present disclosure provides methods and systems for processing a nucleotide mixture. A nucleotide mixture can be purified. A nucleotide mixture can be processed for use in nucleic acid synthesis. A nucleotide mixture can be processed for use in nucleic acid sequencing.

27. 发明授权

US09920364B2 Cyclic single molecule sequencing process 有权
公开(公告)号：US09920364B2
公开(公告)日：2018-03-20
申请号：US14383155
申请日：2013-03-05
申请人： Rudolf Rigler
发明人： Rudolf Rigler
IPC分类号： C12Q1/68 , C07H21/02
CPC分类号： C12Q1/6869 , C12Q1/6874 , C12Q2521/101 , C12Q2521/319 , C12Q2525/125 , C12Q2525/307 , C12Q2531/125 , C12Q2537/155 , C12Q2563/107 , C12Q2565/101 , C12Q2565/518 , C12Q2521/543
摘要： The invention relates to a process for parallel high throughput sequencing of nucleic acid molecules, in particular in the single molecule format.

28. 发明授权

US09605308B2 Alternative nucleotide flows in sequencing-by-synthesis methods 有权
公开(公告)号：US09605308B2
公开(公告)日：2017-03-28
申请号：US13689252
申请日：2012-11-29
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： Jonathan Schultz , John Davidson
IPC分类号： C12Q1/68 , B01L3/00
CPC分类号： C12Q1/6869 , B01L3/5027 , C12Q1/6874 , C12Q2537/155 , C12Q2563/149 , C12Q2565/301 , C12Q2565/629
摘要： A method for sequencing a polynucleotide strand by using sequencing-by-synthesis techniques. To address the problem of incomplete extension (IE) and/or carry forward (CF) errors that can occur in sequencing-by-synthesis reactions, an alternative flow ordering of dNTPs is used. In contrast to conventional flow orderings, the dNTPs are flowed in an ordering that is not a continuous repeat of an ordering of the four different dNTPs. This alternate flow ordering may reduce the loss of phasic synchrony in the population of template polynucleotide strands that result from IE and/or CF errors.

29. 发明申请

US20150196917A1 METHODS AND APPARATUS FOR NUCLEIC ACID SYNTHESIS USING OLIGO-TEMPLATED POLYMERIZATION 审中-公开
标题翻译：使用低分子量聚合法制备核酸合成的方法和装置
公开(公告)号：US20150196917A1
公开(公告)日：2015-07-16
申请号：US14605538
申请日：2015-01-26
申请人： THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY
发明人： Mark A. Kay , Adi Barzel
IPC分类号： B01L7/00
CPC分类号： B01L7/525 , B01L3/50851 , B01L7/52 , B01L2200/0647 , B01L2200/18 , B01L2300/0829 , C07H21/04 , C12Q1/6834 , C12Q1/6844 , C12Q2533/101 , C12Q2537/155 , C12Q2537/157 , C12Q2565/518
摘要： A method and apparatus for synthesizing nucleic acid (NA) by sequential addition of nucleotide triphosphates to a single stranded NA molecule (growing-chain) is described. The growing-chain is annealed to an oligonucleotide, wherein the 3′ end of the oligonucleotide is complementary to the 3′ end of the growing-chain and wherein the protruding 5′ end of the oligonucleotide has the correct sequence to serve as a template for the growing-chain to produce a NA product. The growing-chain is then brought into contact with a polymerase in the presence of one or more nucleotide triphosphates under conditions permitting the polymerase to catalyze addition of one or more nucleotide triphosphates to the 3′ end of the growing-chain using the annealed oligonucleotide as a template. After polymerization has occurred, the annealed growing-chain and oligonucleotide are denatured so that the steps can be repeated until the NA product of desired length is synthesized.
摘要翻译：描述了通过向单链NA分子（生长链）顺序加入核苷酸三磷酸合成核酸（NA）的方法和装置。生长链与寡核苷酸退火，其中寡核苷酸的3'末端与生长链的3'端互补，并且其中寡核苷酸的突出的5'末端具有正确的序列以用作生产NA产品的增长链。然后使生长链在一种或多种核苷酸三磷酸存在的条件下与聚合酶接触，条件是允许聚合酶催化一个或多个核苷酸三磷酸酯加入生长链的3'末端，使用退火寡核苷酸作为一个模板发生聚合后，退火的生长链和寡核苷酸被变性，使得可以重复这些步骤，直到合成所需长度的NA产物。

30. 发明申请

US20130071839A1 SYSTEMS AND METHODS FOR DETECTING BIOMARKERS OF INTEREST 审中-公开
标题翻译：用于检测生物标志物的系统和方法
公开(公告)号：US20130071839A1
公开(公告)日：2013-03-21
申请号：US13603298
申请日：2012-09-04
申请人： Georg Seelig , Barry Lutz
发明人： Georg Seelig , Barry Lutz
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C12Q1/682 , C12Q1/6823 , Y10S977/81 , Y10S977/902 , C12Q2537/1373 , C12Q2537/155 , C12Q2537/157 , C12Q2565/625
摘要： In some embodiments, a strand displacement system is provided. Such a system may include a first nucleic acid catalyst molecule; a nucleic acid gate molecule, wherein the first nucleic acid catalyst molecule binds the nucleic acid gate molecule forming a nucleic acid gate-catalyst complex and releases an output molecule; and a nucleic acid sink molecule. The nucleic acid sink molecule sequesters a putative second nucleic acid catalyst, wherein the second nucleic acid catalyst differs from the first nucleic acid catalyst molecule by at least one nucleotide. In some aspects, the first nucleic acid catalyst may include a biomarker of interest or a nucleic acid aptamer which binds an amino acid-based biomarker of interest.
摘要翻译：在一些实施例中，提供了一种线位移系统。这样的系统可以包括第一核酸催化剂分子; 核酸门分子，其中所述第一核酸催化剂分子结合形成核酸门 - 催化剂复合物的核酸门分子并释放输出分子; 和核酸宿分子。核酸沉积分子螯合假定的第二核酸催化剂，其中第二核酸催化剂与第一核酸催化剂分子不同于至少一个核苷酸。在一些方面，第一核酸催化剂可以包括感兴趣的生物标志物或结合目的基于氨基酸的生物标志物的核酸适体。

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