发明申请
US20150196917A1 METHODS AND APPARATUS FOR NUCLEIC ACID SYNTHESIS USING OLIGO-TEMPLATED POLYMERIZATION
审中-公开

基本信息:
- 专利标题: METHODS AND APPARATUS FOR NUCLEIC ACID SYNTHESIS USING OLIGO-TEMPLATED POLYMERIZATION
- 专利标题(中):使用低分子量聚合法制备核酸合成的方法和装置
- 申请号:US14605538 申请日:2015-01-26
- 公开(公告)号:US20150196917A1 公开(公告)日:2015-07-16
- 发明人: Mark A. Kay , Adi Barzel
- 申请人: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY
- 申请人地址: US CA Palo Alto
- 专利权人: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY
- 当前专利权人: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY
- 当前专利权人地址: US CA Palo Alto
- 主分类号: B01L7/00
- IPC分类号: B01L7/00
摘要:
A method and apparatus for synthesizing nucleic acid (NA) by sequential addition of nucleotide triphosphates to a single stranded NA molecule (growing-chain) is described. The growing-chain is annealed to an oligonucleotide, wherein the 3′ end of the oligonucleotide is complementary to the 3′ end of the growing-chain and wherein the protruding 5′ end of the oligonucleotide has the correct sequence to serve as a template for the growing-chain to produce a NA product. The growing-chain is then brought into contact with a polymerase in the presence of one or more nucleotide triphosphates under conditions permitting the polymerase to catalyze addition of one or more nucleotide triphosphates to the 3′ end of the growing-chain using the annealed oligonucleotide as a template. After polymerization has occurred, the annealed growing-chain and oligonucleotide are denatured so that the steps can be repeated until the NA product of desired length is synthesized.
摘要(中):
描述了通过向单链NA分子(生长链)顺序加入核苷酸三磷酸合成核酸(NA)的方法和装置。 生长链与寡核苷酸退火,其中寡核苷酸的3'末端与生长链的3'端互补,并且其中寡核苷酸的突出的5'末端具有正确的序列以用作 生产NA产品的增长链。 然后使生长链在一种或多种核苷酸三磷酸存在的条件下与聚合酶接触,条件是允许聚合酶催化一个或多个核苷酸三磷酸酯加入生长链的3'末端,使用退火寡核苷酸作为 一个模板 发生聚合后,退火的生长链和寡核苷酸被变性,使得可以重复这些步骤,直到合成所需长度的NA产物。
IPC结构图谱:
B | 作业;运输 |
--B01 | 一般的物理或化学的方法或装置 |
----B01L | 通用化学或物理实验室设备 |
------B01L7/00 | 加热或冷却设备 |