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21. 发明专利

AT497971T 未知
公开(公告)号：AT497971T
公开(公告)日：2011-02-15
申请号：AT00945603
申请日：2000-06-02
申请人： KERN FLORIAN
发明人： KERN FLORIAN , VOLK HANS-DIETER , REINKE PETRA , FAULHABER NICOLE , SUREL INGOLF-PASCAL , KHATAMZAS ELHAM
IPC分类号： C07K14/045 , C12N15/09 , A61K38/00 , A61K39/00 , A61K39/25 , A61P31/12 , C07K7/00 , C12N15/38 , C12N15/63 , G01N33/68
摘要： The present invention relates to peptides or pretide derivatives of the 65kD lower matrix phosphoprotein of human cytomegalovirus. The peptides disclosed are useful for determining whether subjects have een exposed to human cytomegalovirus. Methods for using these peptides or derivatives thereof to determine the peptide-induced production of interferon-gamma and/or TNF-alpha in CD8+ T cells isolated from subjects are also disclosed.

22. 发明专利

DK1257290T3 未知
公开(公告)号：DK1257290T3
公开(公告)日：2009-03-30
申请号：DK01919295
申请日：2001-02-17
申请人： KERN FLORIAN
发明人： KERN FLORIAN , VOLK HANS-DIETER , REINKE PETRA , FAULHABER NICOLE , SUREL INGOLF-PASCAL , KHATAMZAS ELHAM
IPC分类号： A61K39/00 , G01N33/53 , A61K35/26 , A61K38/08 , A61K38/10 , A61K38/16 , A61K39/245 , A61P37/04 , C12N5/06 , C12N5/08 , C12Q1/02 , G01N33/50 , G01N33/68
摘要： The invention relates to a method for the antigen-specific stimulation of T-lymphocytes with a synthetic peptide library, by preparing a plurality of peptides, each of said peptides comprising a fragment less than the whole of the total amino acid sequence of the antigen, each of said peptides being a minimum of 9 amino acid residues (AAs) in length, and the amino acid sequence of each peptide overlapping with the amino acid sequence of at least one other of said peptides; combining a plurality of the peptides from a) into a peptide library, said peptide library comprises a plurality of the peptides selected such that their collective overlapping amino acid sequences span the total amino acid sequence of the antigen; and incubating a suspension comprising CD8+ T lymphocytes, CD4+ T lymphocytes or a mixture of CD8+ and CD4+ with said peptide library in a single culture run.

23. 发明专利

DE50114639D1 未知
公开(公告)号：DE50114639D1
公开(公告)日：2009-02-26
申请号：DE50114639
申请日：2001-02-17
申请人： KERN FLORIAN
发明人： KERN FLORIAN , VOLK HANS-DIETER , REINKE PETRA , FAULHABER NICOLE , SUREL INGOLF-PASCAL , KHATAMZAS ELHAM
IPC分类号： A61K39/00 , G01N33/53 , A61K35/26 , A61K38/08 , A61K38/10 , A61K38/16 , A61K39/245 , A61P37/04 , C12N5/06 , C12N5/08 , C12Q1/02 , G01N33/50 , G01N33/68
摘要： The invention relates to a method for the antigen-specific stimulation of T-lymphocytes with a synthetic peptide library, by preparing a plurality of peptides, each of said peptides comprising a fragment less than the whole of the total amino acid sequence of the antigen, each of said peptides being a minimum of 9 amino acid residues (AAs) in length, and the amino acid sequence of each peptide overlapping with the amino acid sequence of at least one other of said peptides; combining a plurality of the peptides from a) into a peptide library, said peptide library comprises a plurality of the peptides selected such that their collective overlapping amino acid sequences span the total amino acid sequence of the antigen; and incubating a suspension comprising CD8+ T lymphocytes, CD4+ T lymphocytes or a mixture of CD8+ and CD4+ with said peptide library in a single culture run.

24. 发明专利

ES2272074T3 PROCEDIMIENTO PARA LA IDENTIFICACION DE FRAGMENTOS PROTEICOS ESTIMULADORES DE CELULAS T. 未知
公开(公告)号：ES2272074T3
公开(公告)日：2007-04-16
申请号：ES99930888
申请日：1999-01-15
申请人： KERN FLORIAN
发明人： VOLK HANS-DIETER , WALDEN PETER , SCHEFFOLD ALEXANDER , BLASCZYK RAINER , KERN FLORIAN
IPC分类号： G01N33/50 , A61K38/17 , C07K1/02 , C07K1/04 , C07K7/06 , C07K7/08 , C07K14/045 , C12P21/00 , C12Q1/02 , G01N33/68
摘要： Procedimiento para identificar fragmentos proteicos estimuladores de células T que comprende las siguientes etapas: a) determinar la secuencia de aminoácidos de un antígeno, el cual es una proteína o un péptido, b) dividir la secuencia de aminoácidos encontrada del antígeno en fragmentos proteicos, c) sintetizar al menos un fragmento proteico con una longitud de 8 a 30 aminoácidos o escindir la secuencia de aminoácidos del antígeno en al menos un fragmento proteico con una longitud de 8 a 30 aminoácidos, siendo a este respecto el fragmento proteico una secuencia parcial de la secuencia de aminoácidos determinada del antígeno, d) incubar una suspensión que contiene células T con el fragmento o fragmentos proteicos en mezclas de reacción experimentales, e) identificar (i) al menos una citocina de célula T que fue inducida mediante el fragmento o fragmentos proteicos y sintetizada en las células T, estando a este respecto las citocinas de células T presentes en el interior de la célula o unidas a la membrana celular, y/o (ii) al menos un marcador de activación expresando o incrementando en su expresión por el o los fragmentos proteícos debido a la estimulación de las células T que fue inducido por el fragmento o fragmentos proteicos o se incrementó su expresión y se expresa en las células T, pudiendo estar presente a este respecto el marcador de activación en el interior de la célula o expresado en la superficie celular, Identificándose a este respecto la citocina o citocinas de células T o el marcador de activación por citometría de flujo y f) asignar las mezclas de reacción experimentales, en las que se estimularon las células T y se reconoció esta estimulación de células T mediante la identificación de una citocina de células T o varias citocinas de células T y/o uno o varios marcadores de activación a la secuencia o secuencias de aminoácidos de los fragmentos proteicos que se incubaron con las células T, caracterizado porque el tiempo de incubación es tan prolongado que el fragmento o fragmentos proteicos de las moléculas del MHC que se encuentran sobre la superficie celular se captan de forma suficiente, en el que la captación es suficiente cuando es posible una identificación inequívoca de células T estimuladas y porque el tiempo de incubación de la suspensión que contiene células T con el fragmento o fragmentos proteicos es tan corto que no se lleva a cabo una selección y proliferación que vaya acompañada de una eliminación dirigida de determinadas células T.

25. 发明专利

DE10009341A1 未知
公开(公告)号：DE10009341A1
公开(公告)日：2001-09-06
申请号：DE10009341
申请日：2000-02-22
申请人： KERN FLORIAN
发明人： KERN FLORIAN , VOLK HANS-DIETER , REINKE PETRA , FAULHABER NICOLE , SUREL INGOLF-PASCAL , KHATAMAZAS ELHAM
IPC分类号： G01N33/53 , A61K35/26 , A61P37/04 , C12N5/06 , C12Q1/02 , G01N33/50 , A61K39/39
摘要： The invention relates to a method for the antigen-specific stimulation of T-lymphocytes with synthetic peptide libraries, comprising the following steps: a) dividing the whole amino acid sequence of the antigen into protein fragments with partial amino acid sequences; b) synthesising a peptide library containing these protein fragments; c) incubating a suspension containing CD8- and/or CD4-T-lymphocytes with all of the protein fragments of the peptide library in a single culture batch. The method can be used for immunostimulation of T-lymphocytes of mammals, especially humans, as well as for diagnosis purposes, in order to determine whether a mammal, especially a human being, has previously responded to a specific protein with its/his/her immune system, and the strength of this response.

26. 发明专利

DE19927039A1 New peptide compounds including Immediate-Early 1 Protein fragments, useful for the production of vaccines against human cytomegaloviruses (HCMV) and diagnostics for the identification of HCMV infections 未知
公开(公告)号：DE19927039A1
公开(公告)日：2000-12-07
申请号：DE19927039
申请日：1999-06-04
申请人： KERN FLORIAN
发明人： KERN FLORIAN , VOLK HANS-DIETER , REINKE PETRA , FAULHABER NICOLE , SUREL INGOLF-PASCAL , KHATAMZAS ELHAM
IPC分类号： A61K38/00 , C07K7/06 , C07K14/045 , A61K38/08 , A61K38/10 , C07K7/08
摘要： Peptides (including Immediate-Early 1 Protein (IE-1) fragments) and their derivatives resulting from deletions, insertions or substitutions are new. New peptides comprise sequences (a)-(l) and their derivatives result from a deletion, insertion or substitution of 1-3 amino acids of these sequences and have the function of SerTyrPheProGluIleThrHisIle to induce the secretion of interferon- psi in CD8 cells. RN-SerTyrPheProGluIleThrHisIle-RC (a) RN-IE-1037-051-RC (b) RN-IE-1073-087-RC (c) RN-IE-1085-099-RC (d) RN-IE-1181-195-RC (e) RN-IE-1193-207-RC (= RN-AlaLysAlaLysLysA- spGluLeuArgArgLysMetMetTyrMet-RC) (f) RN-IE-1198-207-RC (= RN-AspGluLeuArgArgLysMetMetTyrMet-RC) (g) RN-IE-1199-213-RC (=RN-GluLeuArgLysMetMetTyrMetCysTyrArgAsnIleGlu-RC) (h) RN-IE-1289-303-RC (i) RN-IE-1307-321-RC (=RN-GluPheCysArgValLeuCysCysTyrValLeuGluGluThrSer-RC (j) RN-IE-1379-393-RC (k) RN-IE-1421-435-RC (l) RN = H; a NH2 protecting group; or 1 or more additional amino acids; RC = OH; a COOH protecting group; or 1 or more additional amino acids; IE-1nnn-nnn = sequence from the IE-1 protein corresponding to amino acids in the nnn to nnn position of IE-1

27. 发明申请

WO0075180A3 PEPTIDES FOR VACCINATING AGAINST HUMAN CMV 审中-公开
标题翻译：肽疫苗接种反对人类巨细胞病毒
公开(公告)号：WO0075180A3
公开(公告)日：2001-07-26
申请号：PCT/DE0001854
申请日：2000-06-02
申请人： KERN FLORIAN , VOLK HANS DIETER , REINKE PETRA , FAULHABER NICOLE , SUREL INGOLF PASCAL , KHATAMZAS ELHAM
发明人： KERN FLORIAN , VOLK HANS-DIETER , REINKE PETRA , FAULHABER NICOLE , SUREL INGOLF-PASCAL , KHATAMZAS ELHAM
IPC分类号： C12N15/09 , A61K38/00 , A61K39/00 , A61K39/25 , A61P31/12 , C07K7/00 , C07K14/045 , C12N15/38 , C12N15/63 , G01N33/68
CPC分类号： C07K14/005 , A61K39/00 , C12N2710/16122 , C12N2710/16134 , Y02P20/55
摘要： The invention relates to peptides which are optionally fragments of the IE-1 or pp65 protein selected from the following group having sequence RN - Gln Thr Met Leu Arg Lys Glu Val Asn Ser Gln Leu Ser Leu Gly - RC; RN - Cys Asn Glu Asn Pro Glu Lys Asp Val Leu Ala Glu Leu Val Lys - RC; RN - Leu Val Lys Gln Ile Lys Val Arg Val Asp Met Val Arg His Arg - RC; RN - Ala Ala Asn Lys Leu Gly Gly Ala Leu Gln Ala Lys Ala Arg Ala - RC; RN - Ala Arg Ala Lys Lys Asp Glu Leu Arg Arg Lys Met Met Tyr Met - RC; RN - Asp Glu Leu Arg Arg Lys Met Met Tyr Met - RC; RN - Glu Leu Arg Arg Lys Met Met Tyr Met Cys Tyr Arg Asn Ile Glu - RC; RN - Val Thr Ser Asp Ala Cys Met Met Thr Met Tyr Gly Gly Ile Ser - RC; RN - Glu Phe Cys Arg Val Leu Cys Cys Tyr Val Leu Glu Glu Thr Ser - RC; RN - Met Ser Ile Tyr Val Tyr Ala Leu Pro Leu Lys Met Leu Asn Ile - RC; RN - Val Tyr Ala Leu Pro Leu Lys Met Leu Asn Ile Pro Ser Ile Asn - RC; RN - Ala Leu Pro Leu Lys Met Leu Asn Ile - RC; RN - His Ile Met Leu Asp Val Ala Phe Thr Ser His Glu His Phe Gly RC; RN - Asp Val Ala Phe Thr Ser His Glu His Phe Gly Leu Leu Cys Pro - RC; RN - Val Ala Phe Thr Ser His Glu His Phe - RC; RN - Ala Phe Thr Ser His Glu His Phe Gly - RC; RN - Ala Asn Asp Ile Tyr Arg Ile Phe Ala Glu Leu Glu Gly Val Trp - RC; RN - Val Cys Ser Met Glu Asn Thr Arg Ala Thr Lys Met Gln Val Ile - RC; RN - Glu Asn Thr Arg Ala Thr Lys Met Gln Val Ile Gly Asp Gln Tyr - RC; RN - Asn Thr Arg Ala Thr Lys Met Gln Val - RC; RN - Thr Arg Ala Thr Lys Met Gln Val Ile - RC; RN - Gln Pro Phe Met Arg Pro His Glu Arg Asn Gly Phe Thr Val Leu - RC; RN - Pro Leu Lys Met Leu Asn Ile Pro Ser Ile Asn Val His His Tyr - RC; RN - Leu Asn Ile Pro Ser Ile Asn Val His His Tyr Pro Ser Ala Ala - RC; RN Glu Asp Val Pro Ser Glu Lys Leu Phe Met His Val Thr Leu Gly - RC; RN - Cys Arg Val Leu Cys Cys Tyr Val Leu - RC; RN - Arg Val Leu Cys Cys Tyr Val Leu Glu - RC; RN - Val Leu Cys Cys Tyr Val Leu Glu Glu - RC; RN - Glu Leu Arg Arg Lys Met Met Tyr Met - RC; RN - Asp Glu Leu Arg Arg Lys Met Met Tyr - RC; RN Asp Glu Leu Arg Arg Lys Met Met Tyr Met - RC; RN - Asp Glu Glu Glu Ala Ile Val Ala Tyr Tyr Leu Ala Thr Ala Gly - RC or RN - Glu Asn Ser Asp Gln Glu Ser Glu Gln Ser Asp Glu Glu Glu - RC, whereby RN represents -H or an amino protecting group; RC represents -OH or a carboxyl protecting group. The inventive peptides are used in the preparation of a medicaments for vaccination against HVMV infections or a diagnostic reagent for identifying an immune response against HCMV.
摘要翻译：肽，其是IE-1蛋白或病毒pp65的任选片段，选自下组，其具有序列：RN - 谷氨酰胺苏氨酸亮氨酸蛋氨酸赖氨酸精氨酸谷氨酸的Asn缬氨酸丝氨酸谷氨酰胺亮氨酸亮氨酸丝氨酸甘氨酸 - RC; RN - 半胱氨酸天冬酰胺谷氨酸天冬酰胺临谷氨酸天冬氨酸赖氨酸缬氨酸丙氨酸谷氨酸亮氨酸亮氨酸缬氨酸赖氨酸 - RC; RN - 亮氨酸缬氨酸赖氨酸谷氨酰胺异亮氨酸赖氨酸精氨酸瓦尔瓦尔会见瓦尔天冬氨酸精氨酸他精氨酸 - RC; RN - 丙氨酸丙氨酸天冬酰胺赖氨酸列伊甘氨酸甘氨酸丙氨酸亮氨酸谷氨酰胺丙氨酸赖氨酸丙氨酸精氨酸丙氨酸 - RC; RN - 丙氨酸精氨酸丙氨酸赖氨酸赖氨酸天冬氨酸谷氨酸列伊ARG ARG赖氨酸蛋氨酸的Met酪氨酸蛋氨酸 - RC; RN - 天冬氨酸谷氨酸列伊ARG ARG赖氨酸蛋氨酸的Met酪氨酸蛋氨酸 - RC; RN - 谷氨酸列伊ARG ARG赖氨酸蛋氨酸的Met酪氨酸蛋氨酸半胱氨酸酪氨酸天冬酰胺精氨酸谷氨酸岛 - RC; RN - 瓦尔苏氨酸丝氨酸天冬氨酸丙氨酸半胱氨酸苏氨酸蛋氨酸蛋氨酸蛋氨酸酪氨酸甘氨酸甘氨酸丝氨酸岛 - RC; RN - 谷氨酸苯丙氨酸半胱氨酸精氨酸瓦尔·莱半胱氨酸半胱氨酸酪氨酸瓦尔·莱谷氨酸谷氨酸苏氨酸丝氨酸 - RC; RN - 蛋氨酸序列异亮氨酸酪氨酸酪氨酸丙氨酸瓦尔·莱临赖氨酸列伊列伊蛋氨酸天冬酰胺岛 - RC; RN - 瓦尔酪氨酸亮氨酸丙氨酸临列伊赖氨酸列伊蛋氨酸天冬酰胺岛临天冬酰胺丝氨酸岛 - RC; RN - 阿拉列伊临亮氨酸赖氨酸蛋氨酸亮氨酸天冬酰胺岛 - RC; RN - 他的异亮氨酸天冬氨酸列伊会见瓦尔丙氨酸苯丙氨酸苏氨酸丝氨酸谷氨酸他的苯丙氨酸甘氨酸他的RC; RN - 天冬氨酸缬氨酸丙氨酸苯丙氨酸苏氨酸丝氨酸谷氨酸他对他的苯丙氨酸亮氨酸亮氨酸甘氨酸半胱氨酸临 - RC; RN - 瓦尔丙氨酸苯丙氨酸苏氨酸丝氨酸谷氨酸他对他的苯丙氨酸 - RC; RN - 丙氨酸苯丙氨酸苏氨酸丝氨酸谷氨酸他对他的苯丙氨酸甘氨酸 - RC; RN - 丙氨酸天冬酰胺天冬氨酸异亮氨酸酪氨酸精氨酸异亮氨酸苯丙氨酸丙氨酸亮氨酸谷氨酸谷氨酸甘氨酸缬氨酸色氨酸 - RC; RN - 瓦尔丝氨酸半胱氨酸蛋氨酸谷氨酸苏氨酸天冬酰胺精氨酸丙氨酸苏氨酸谷氨酰胺赖氨酸会见瓦尔岛 - RC; RN - 谷氨酸天冬酰胺苏氨酸丙氨酸苏氨酸精氨酸赖氨酸蛋氨酸谷氨酰胺异亮氨酸缬氨酸甘氨酸天冬氨酸谷氨酰胺酪氨酸 - RC; RN - 天冬酰胺苏氨酸精氨酸苏氨酸丙氨酸赖氨酸谷氨酰胺会见瓦尔 - RC; RN - 苏氨酸精氨酸苏氨酸丙氨酸赖氨酸谷氨酰胺会见瓦尔岛 - RC; RN - 谷氨酰胺临苯丙氨酸精氨酸会见临他天冬酰胺谷氨酸精氨酸甘氨酸苯丙氨酸苏氨酸瓦尔·莱 - RC; RN - 临亮氨酸赖氨酸蛋氨酸亮氨酸天冬酰胺岛临丝氨酸天冬酰胺异亮氨酸缬氨酸他对他的酪氨酸 - RC; RN - 亮氨酸天冬酰胺岛临丝氨酸天冬酰胺异亮氨酸缬氨酸他对他的酪氨酸丝氨酸临丙氨酸丙氨酸 - RC; RN谷氨酸天冬氨酸丝氨酸缬氨酸临谷氨酸赖氨酸列伊苯丙氨酸遇见了他的Val苏氨酸亮氨酸甘氨酸 - RC; RN - 半胱氨酸精氨酸瓦尔·莱半胱氨酸半胱氨酸酪氨酸瓦尔·莱 - RC; RN - 精氨酸瓦尔·莱半胱氨酸半胱氨酸酪氨酸瓦尔·莱谷氨酸 - RC; RN - 瓦尔·莱半胱氨酸半胱氨酸酪氨酸瓦尔·莱谷氨酸谷氨酸 - RC; RN - 谷氨酸列伊ARG ARG赖氨酸蛋氨酸的Met酪氨酸蛋氨酸 - RC; RN - 天冬氨酸谷氨酸列伊ARG ARG赖氨酸蛋氨酸的Met酪氨酸 - RC; RN - 天冬氨酸谷氨酸列伊ARG ARG赖氨酸蛋氨酸的Met酪氨酸蛋氨酸 - RC; RN - 天冬氨酸谷氨酸谷氨酸谷氨酸丙氨酸异亮氨酸缬氨酸丙氨酸酪氨酸酪氨酸亮氨酸丙氨酸苏氨酸丙氨酸甘氨酸 - RC或RN - 谷氨酸的Asn丝氨酸天冬氨酸谷氨酰胺谷氨酸丝氨酸谷氨酸谷氨酰胺丝氨酸天冬氨酸谷氨酸谷氨酸谷氨酸 - RC焦点是RN是-H或一个氨基保护基; RC -OH或羧基保护基团。新颖的肽用于制备药物的免疫接种抗感染HVMV或诊断剂，以确定针对HCMV的免疫应答。

28. 发明申请

WO0163286A3 METHOD FOR ANTIGEN-SPECIFIC STIMULATION OF T-LYMPHOCYTES WITH SYNTHETIC PEPTIDE LIBRARIES 审中-公开
标题翻译：方法用合成肽库的T淋巴细胞抗原特异性刺激
公开(公告)号：WO0163286A3
公开(公告)日：2002-03-14
申请号：PCT/EP0101773
申请日：2001-02-17
申请人： KERN FLORIAN , VOLK HANS DIETER , REINKE PETRA , FAULHABER NICOLE , SUREL INGOLF PASCAL , KHATAMZAS ELHAM
发明人： KERN FLORIAN , VOLK HANS-DIETER , REINKE PETRA , FAULHABER NICOLE , SUREL INGOLF-PASCAL , KHATAMZAS ELHAM
IPC分类号： G01N33/53 , A61K35/26 , A61P37/04 , C12N5/06 , C12Q1/02 , G01N33/50 , A61K39/00 , A61K38/08 , A61K38/10 , A61K38/16 , A61K39/245 , C12N5/08 , G01N33/68
CPC分类号： G01N33/505
摘要： The invention relates to a method for the antigen-specific stimulation of T-lymphocytes with synthetic peptide libraries, comprising the following steps: a) dividing the whole amino acid sequence of the antigen into protein fragments with partial amino acid sequences; b) synthesising a peptide library containing these protein fragments; c) incubating a suspension containing CD8- and/or CD4-T-lymphocytes with all of the protein fragments of the peptide library in a single culture batch. The method can be used for immunostimulation of T-lymphocytes of mammals, especially humans, as well as for diagnosis purposes, in order to determine whether a mammal, especially a human being, has previously responded to a specific protein with its/his/her immune system, and the strength of this response.
摘要翻译：本发明涉及一种用于用合成肽文库的T淋巴细胞的抗原特异性刺激的方法，包括以下步骤：a）将抗原的总氨基酸序列与部分氨基酸序列的蛋白片段，b）合成含有这些蛋白片段的肽文库 ; c）中温育CD8和/或含有该肽库的所有蛋白质片段CD4 + T淋巴细胞悬浮在单一培养物制备。该方法既可以用于哺乳动物，特别是人类的T淋巴细胞的免疫刺激以及用于诊断来确定是否哺乳动物，尤其是人，已响应特定的蛋白质，其免疫系统有多强这个答案，

29. 发明申请

WO9936568A3 METHOD FOR IDENTIFYING T-CELL STIMULATING PROTEIN FRAGMENTS 审中-公开
标题翻译：一种鉴定T细胞刺激蛋白片段
公开(公告)号：WO9936568A3
公开(公告)日：2000-04-06
申请号：PCT/DE9900175
申请日：1999-01-15
申请人： KERN FLORIAN , VOLK HANS DIETER , WALDEN PETER , SCHEFFOLD ALEXANDER , BLASCZYK RAINER
发明人： KERN FLORIAN , VOLK HANS-DIETER , WALDEN PETER , SCHEFFOLD ALEXANDER , BLASCZYK RAINER
IPC分类号： C07K1/02 , C07K1/04 , C07K7/06 , C07K7/08 , C07K14/045 , C12Q1/02 , G01N33/68 , G01N33/50 , A61K38/17 , C12P21/00
CPC分类号： G01N33/6863
摘要： The invention relates to a method for identifying T-cell stimulating protein fragments using the following steps: a) detecting the amino acid sequence of an antigen; b) subdividing the found amino acid sequence of the antigen into protein fragments; c) synthesizing at least one protein fragment; d) incubating a suspension containing t-cells with the protein fragments; e) identifying an induced T-cell cytokine or activation marker by flow-through cytometry, and; f) assigning the T-cells, with which T-cell cytokines and/or activation markers were identified, to the protein fragments which were incubated with the T-cells. The corresponding protein fragments/peptides are synthetically produced with the assistance of the detected positive sequence, and said corresponding protein fragments/peptides can be utilized to produce a medicament for immunostimulation.
摘要翻译：本发明涉及用于鉴定T细胞刺激蛋白片段，包括以下步骤的方法：a）确定的抗原的氨基酸序列，b）将所述抗原的发现氨基酸序列导入蛋白质片段，c）中合成至少一种蛋白片段d）培养一含T细胞悬浮液与蛋白质的片段，e）鉴定诱导T细胞的细胞因子或活化标记，通过流式细胞术，以及f）分配所述T细胞，其中T细胞的细胞因子和/或活化标记物鉴定为蛋白质片段，其温育与T细胞。与所确定的正序的辅助下的相应蛋白片段/肽合成制备，并且可以被用于免疫刺激的药物中的用途。

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