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1. 发明专利

DE19834932A1 Identifying T-cell stimulating fragments of antigenic proteins or peptides, useful as immunostimulants for vaccination or desensitization 未知
公开(公告)号：DE19834932A1
公开(公告)日：2000-06-15
申请号：DE19834932
申请日：1998-07-28
申请人： KERN FLORIAN , BLASCZYK RAINER , SCHEFFOLD ALEXANDER , VOLK HANS DIETER , WALDEN PETER
发明人： KERN FLORIAN , BLASCZYK RAINER , SCHEFFOLD ALEXANDER , VOLK HANS-DIETER , WALDEN PETER
IPC分类号： G01N33/68 , C12Q1/02 , C12P21/00
摘要： A method for identifying T-cell stimulating protein fragments, is new. Identification of T cell stimulatory protein fragments (I) comprises incubating a suspension containing T cells with at least one fragment (of 8-30 amino acids (aa)) from an antigenic protein or peptide, then determining, by flow cytometry, either at least one T-cell cytokine and/or at least one activation marker (both cytokines and markers being intracellular or on the cell surface). Fragments that cause T cell activation are selected. Initially the sequence of an antigen is determined and the sequence divided into fragments which are then synthesized or produced by cleavage of the antigen. An Independent claim is also included for production of T-cell stimulatory (I) identified this way.

2. 发明申请

WO9936568A3 METHOD FOR IDENTIFYING T-CELL STIMULATING PROTEIN FRAGMENTS 审中-公开
标题翻译：一种鉴定T细胞刺激蛋白片段
公开(公告)号：WO9936568A3
公开(公告)日：2000-04-06
申请号：PCT/DE9900175
申请日：1999-01-15
申请人： KERN FLORIAN , VOLK HANS DIETER , WALDEN PETER , SCHEFFOLD ALEXANDER , BLASCZYK RAINER
发明人： KERN FLORIAN , VOLK HANS-DIETER , WALDEN PETER , SCHEFFOLD ALEXANDER , BLASCZYK RAINER
IPC分类号： C07K1/02 , C07K1/04 , C07K7/06 , C07K7/08 , C07K14/045 , C12Q1/02 , G01N33/68 , G01N33/50 , A61K38/17 , C12P21/00
CPC分类号： G01N33/6863
摘要： The invention relates to a method for identifying T-cell stimulating protein fragments using the following steps: a) detecting the amino acid sequence of an antigen; b) subdividing the found amino acid sequence of the antigen into protein fragments; c) synthesizing at least one protein fragment; d) incubating a suspension containing t-cells with the protein fragments; e) identifying an induced T-cell cytokine or activation marker by flow-through cytometry, and; f) assigning the T-cells, with which T-cell cytokines and/or activation markers were identified, to the protein fragments which were incubated with the T-cells. The corresponding protein fragments/peptides are synthetically produced with the assistance of the detected positive sequence, and said corresponding protein fragments/peptides can be utilized to produce a medicament for immunostimulation.
摘要翻译：本发明涉及用于鉴定T细胞刺激蛋白片段，包括以下步骤的方法：a）确定的抗原的氨基酸序列，b）将所述抗原的发现氨基酸序列导入蛋白质片段，c）中合成至少一种蛋白片段d）培养一含T细胞悬浮液与蛋白质的片段，e）鉴定诱导T细胞的细胞因子或活化标记，通过流式细胞术，以及f）分配所述T细胞，其中T细胞的细胞因子和/或活化标记物鉴定为蛋白质片段，其温育与T细胞。与所确定的正序的辅助下的相应蛋白片段/肽合成制备，并且可以被用于免疫刺激的药物中的用途。

3. 发明申请

WO2010091679A3 USE OF NOTCH REGULATORS FOR MODULATING THE IMMUNE RESPONSE BY INDUCTION/SUPPRESSION OF INTERLEUKIN-22 审中-公开
标题翻译： NOTCH调节物质调制免疫应答白细胞介素22的感应/抑制的用途
公开(公告)号：WO2010091679A3
公开(公告)日：2010-11-25
申请号：PCT/DE2010000212
申请日：2010-02-16
申请人： DEUTSCHES RHEUMA FORSCHUNGSZENTRUM BERLIN , SCHEFFOLD ALEXANDER , RUTZ SASCHA , XHEINRICH FREDERICK
发明人： SCHEFFOLD ALEXANDER , RUTZ SASCHA , XHEINRICH FREDERICK
IPC分类号： A61K38/16 , A61K39/395 , A61P29/00 , A61P35/00
CPC分类号： A61K38/16
摘要： The invention relates to the use of notch regulators for modulating IL-22 production in T-cells, by influencing the activity or activation of the notch signal path. The invention further relates to the use for modulating the immune response, primarily in case of infection reactions. The invention in particular relates to the use for treating illnesses associated with infections. The invention further relates to the use for reducing IL-22 production in T-cells.
摘要翻译：本发明涉及使用的Notch调节通过影响活性或Notch信号传导途径的激活来调节IL-22生产中的T细胞。本发明还涉及使用调节免疫应答，特别是在炎性反应。特别地，本发明涉及使用用于治疗与炎症相关的疾病的治疗。外的本发明涉及使用对IL-22生产的T细胞的减少。

4. 发明申请

WO2008092445A3 METHOD FOR MODULATING THE IMMUNE RESPONSE BY ACTIVATING OR INACTIVATING THE NOTCH AND/OR STAT4 SIGNAL PATH 审中-公开
标题翻译：调制免疫反应的方法用激活或失活缺口和/或STAT4信号路径
公开(公告)号：WO2008092445A3
公开(公告)日：2008-10-09
申请号：PCT/DE2008000210
申请日：2008-01-30
申请人： DEUTSCHES RHEUMA FORSCHUNGSZEN , SCHEFFOLD ALEXANDER
发明人： SCHEFFOLD ALEXANDER
IPC分类号： C12N5/0783
CPC分类号： C12N5/0636 , A61K2035/122 , C12N2501/23 , C12N2501/42 , C12N2501/51 , C12N2501/515
摘要： The invention relates to a method for modulating the inflammatory potential of T cells, particularly by regulating the IL-10 production in pro-inflammatory T cells by influencing the activity or the activation of the notch and STAT-4 signal path. The invention further relates to the use of said method for controlling inflammations or for immune suppression. The invention particularly relates to the use of said method for treating diseases associated with inflammations. The invention also relates to the use of said method for reducing the IL-10 production in pro-inflammatory T cells and thus reinforcing T cell-induced immune response, especially in case of infections, tumor diseases, or vaccinations against infections or tumors.
摘要翻译：本发明通过在促炎性T细胞产生IL-10的调节涉及T细胞的炎症潜能的调制的方法，特别是，通过影响活性或Notch和STAT-4信号传导途径的活化。本发明还涉及使用该方法的用于抑制炎症或用于免疫抑制，特别是，本发明涉及使用该方法的用于治疗与炎症相关的疾病的治疗。此外，本发明涉及使用IL-10产生促炎T细胞的方法，并因此增强T细胞介导的免疫应答，特别是在感染，肿瘤疾病或疫苗接种抗感染或肿瘤减少的。

5. 发明申请

WO0173117A9 USE OF PARTICLES WHICH HAVE SIGNAL-EMITTING CHARACTERISTICS AND AT LEAST ONE GROUP WITH AN AFFINITY FOR MARKED NUCLEIC ACIDS 审中-公开
标题翻译：使用该信号的影像学特征和至少出现一个分组亲和力标记的核酸OWN粒子的
公开(公告)号：WO0173117A9
公开(公告)日：2002-07-18
申请号：PCT/EP0103702
申请日：2001-03-31
申请人： MEMOREC MEDICAL MOLECULAR RES , BOSIO ANDREAS , SCHEFFOLD ALEXANDER
发明人： BOSIO ANDREAS , SCHEFFOLD ALEXANDER
IPC分类号： G01N33/53 , C12M1/00 , C12M1/34 , C12N15/09 , C12Q1/68 , G01N33/543 , G01N33/544 , G01N33/566 , G01N37/00
CPC分类号： C12Q1/6804 , C12Q2563/161 , C12Q2563/107
摘要： The invention relates to the use of particles with signal-emitting characteristics and at least one group with an affinity for marked nucleic acids, for detecting marked nucleic acids which are hybridised with nucleic acids that are immobilised on a surface. The invention also relates to supports containing particles which have signal-emitting characteristics and at least one group with an affinity for marked nucleic acids, for detecting marked nucleic acids.
摘要翻译：使用与信号产生特性和用于标记的核酸为标记的核酸的检测，其杂交至固定化的核酸的表面上的至少一个亲和基团的粒子。也有颗粒时，信号产生特性和用于标记的核酸用于检测的标记的核酸，其是本发明的主题的至少一个亲和基团的支持。

6. 发明专利

ES2272074T3 PROCEDIMIENTO PARA LA IDENTIFICACION DE FRAGMENTOS PROTEICOS ESTIMULADORES DE CELULAS T. 未知
公开(公告)号：ES2272074T3
公开(公告)日：2007-04-16
申请号：ES99930888
申请日：1999-01-15
申请人： KERN FLORIAN
发明人： VOLK HANS-DIETER , WALDEN PETER , SCHEFFOLD ALEXANDER , BLASCZYK RAINER , KERN FLORIAN
IPC分类号： G01N33/50 , A61K38/17 , C07K1/02 , C07K1/04 , C07K7/06 , C07K7/08 , C07K14/045 , C12P21/00 , C12Q1/02 , G01N33/68
摘要： Procedimiento para identificar fragmentos proteicos estimuladores de células T que comprende las siguientes etapas: a) determinar la secuencia de aminoácidos de un antígeno, el cual es una proteína o un péptido, b) dividir la secuencia de aminoácidos encontrada del antígeno en fragmentos proteicos, c) sintetizar al menos un fragmento proteico con una longitud de 8 a 30 aminoácidos o escindir la secuencia de aminoácidos del antígeno en al menos un fragmento proteico con una longitud de 8 a 30 aminoácidos, siendo a este respecto el fragmento proteico una secuencia parcial de la secuencia de aminoácidos determinada del antígeno, d) incubar una suspensión que contiene células T con el fragmento o fragmentos proteicos en mezclas de reacción experimentales, e) identificar (i) al menos una citocina de célula T que fue inducida mediante el fragmento o fragmentos proteicos y sintetizada en las células T, estando a este respecto las citocinas de células T presentes en el interior de la célula o unidas a la membrana celular, y/o (ii) al menos un marcador de activación expresando o incrementando en su expresión por el o los fragmentos proteícos debido a la estimulación de las células T que fue inducido por el fragmento o fragmentos proteicos o se incrementó su expresión y se expresa en las células T, pudiendo estar presente a este respecto el marcador de activación en el interior de la célula o expresado en la superficie celular, Identificándose a este respecto la citocina o citocinas de células T o el marcador de activación por citometría de flujo y f) asignar las mezclas de reacción experimentales, en las que se estimularon las células T y se reconoció esta estimulación de células T mediante la identificación de una citocina de células T o varias citocinas de células T y/o uno o varios marcadores de activación a la secuencia o secuencias de aminoácidos de los fragmentos proteicos que se incubaron con las células T, caracterizado porque el tiempo de incubación es tan prolongado que el fragmento o fragmentos proteicos de las moléculas del MHC que se encuentran sobre la superficie celular se captan de forma suficiente, en el que la captación es suficiente cuando es posible una identificación inequívoca de células T estimuladas y porque el tiempo de incubación de la suspensión que contiene células T con el fragmento o fragmentos proteicos es tan corto que no se lleva a cabo una selección y proliferación que vaya acompañada de una eliminación dirigida de determinadas células T.

7. 发明专利

DK1051619T3 未知
公开(公告)号：DK1051619T3
公开(公告)日：2006-12-11
申请号：DK99930888
申请日：1999-01-15
申请人： KERN FLORIAN
发明人： WALDEN PETER , VOLK HANS-DIETER , SCHEFFOLD ALEXANDER , BLASCZYK RAINER , KERN FLORIAN
IPC分类号： G01N33/50 , A61K38/17 , C07K1/02 , C07K1/04 , C07K7/06 , C07K7/08 , C07K14/045 , C12P21/00 , C12Q1/02 , G01N33/68
摘要： The invention relates to a method for identifying T-cell stimulating protein fragments using the following steps: a) detecting the amino acid sequence of an antigen; b) subdividing the found amino acid sequence of the antigen into protein fragments; c) synthesizing at least one protein fragment; d) incubating a suspension containing t-cells with the protein fragments; e) identifying an induced T-cell cytokine or activation marker by flow-through cytometry, and; f) assigning the T-cells, with which T-cell cytokines and/or activation markers were identified, to the protein fragments which were incubated with the T-cells. The corresponding protein fragments/peptides are synthetically produced with the assistance of the detected positive sequence, and said corresponding protein fragments/peptides can be utilized to produce a medicament for immunostimulation.

8. 发明专利

DE59913761D1 未知
公开(公告)号：DE59913761D1
公开(公告)日：2006-09-21
申请号：DE59913761
申请日：1999-01-15
申请人： KERN FLORIAN
发明人： VOLK HANS-DIETER , WALDEN PETER , SCHEFFOLD ALEXANDER , BLASCZYK RAINER , KERN FLORIAN
IPC分类号： G01N33/50 , A61K38/17 , C07K1/02 , C07K1/04 , C07K7/06 , C07K7/08 , C07K14/045 , C12P21/00 , C12Q1/02 , G01N33/68
摘要： The invention relates to a method for identifying T-cell stimulating protein fragments using the following steps: a) detecting the amino acid sequence of an antigen; b) subdividing the found amino acid sequence of the antigen into protein fragments; c) synthesizing at least one protein fragment; d) incubating a suspension containing t-cells with the protein fragments; e) identifying an induced T-cell cytokine or activation marker by flow-through cytometry, and; f) assigning the T-cells, with which T-cell cytokines and/or activation markers were identified, to the protein fragments which were incubated with the T-cells. The corresponding protein fragments/peptides are synthetically produced with the assistance of the detected positive sequence, and said corresponding protein fragments/peptides can be utilized to produce a medicament for immunostimulation.

9. 发明专利

DE19980037D2 未知
公开(公告)号：DE19980037D2
公开(公告)日：2001-03-22
申请号：DE19980037
申请日：1999-01-15
申请人： KERN FLORIAN
发明人： VOLK HANS-DIETER , WALDEN PETER , SCHEFFOLD ALEXANDER , BLASCZYK REINER
IPC分类号： C07K1/02 , C07K1/04 , C07K7/06 , C07K7/08 , C07K14/045 , C12Q1/02 , G01N33/68 , C12Q1/00
摘要： The invention relates to a method for identifying T-cell stimulating protein fragments using the following steps: a) detecting the amino acid sequence of an antigen; b) subdividing the found amino acid sequence of the antigen into protein fragments; c) synthesizing at least one protein fragment; d) incubating a suspension containing t-cells with the protein fragments; e) identifying an induced T-cell cytokine or activation marker by flow-through cytometry, and; f) assigning the T-cells, with which T-cell cytokines and/or activation markers were identified, to the protein fragments which were incubated with the T-cells. The corresponding protein fragments/peptides are synthetically produced with the assistance of the detected positive sequence, and said corresponding protein fragments/peptides can be utilized to produce a medicament for immunostimulation.

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