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21. 发明授权

US06184007B2 Method for the fermentative production of D-pantothenic acid by enhancement of the panD gene in microorganisms 失效
标题翻译：通过增加微生物中panD基因来发酵D-泛酸的方法
公开(公告)号：US06184007B2
公开(公告)日：2001-02-06
申请号：US09318793
申请日：1999-05-26
申请人： Nicole Dusch , Jörn Kalinowski , Alfred Puhler
发明人： Nicole Dusch , Jörn Kalinowski , Alfred Puhler
IPC分类号： C12P1300
CPC分类号： C12N9/88 , C12N15/52 , C12P13/02
摘要： The invention is a method for the production of D-pantothenic acid by the fermentation of microorganisms in which at least the panD gene is enhanced (overexpressed), optionally in combination with the panB and/or panC gene, to provide for the enrichment of the pantothenic acid in the medium or the cells of the microorganisms.
摘要翻译：本发明是通过微生物的发酵来生产D-泛酸的方法，其中至少panD基因被增强（过表达），任选与panB和/或panC基因组合，以提供丰富的培养基中的泛酸或微生物的细胞。

22. 发明授权

US4673642A Plasmid P SVH 1 and its use 失效
标题翻译：质粒P SVH 1及其用途
公开(公告)号：US4673642A
公开(公告)日：1987-06-16
申请号：US642742
申请日：1984-08-20
申请人： Alfred Puhler , Wolfgang Wolleben , Michael Leineweber
发明人： Alfred Puhler , Wolfgang Wolleben , Michael Leineweber
IPC分类号： C12N15/09 , C07H21/04 , C12N15/76 , C12P19/34 , C12R1/465 , C12R1/61 , C12N1/00 , C12N1/20 , C12N15/00 , C12P21/00
CPC分类号： C12N15/76 , Y10S435/886 , Y10S435/906
摘要： The plasmid p SVH 1, which is obtained from Streptomyces venezuelae DSM 40755 and which has a molecular weight of 8.4 megadaltons, a contour length of 4.1 .mu.m and a molecular size of 12.6 kilobases, and its use for the construction of a vector and for cloning foreign DNA in suitable host organisms.
摘要翻译：得自Streptomyces venezuelae DSM 40755，分子量为8.4兆道尔顿，轮廓长度为4.1μm，分子大小为12.6千碱基的质粒p SVH 1及其用于构建载体和在适当的宿主生物体内克隆外来DNA。

23. 发明授权

US5175108A Plasmids from corynebacterium glutamicum and plasmid vectors derived therefrom 失效
标题翻译：来自CORYNERBACTERIUM GLUTAMICUM的PLASMIDS和其衍生的PLASMID VECTOR
公开(公告)号：US5175108A
公开(公告)日：1992-12-29
申请号：US750043
申请日：1991-08-27
申请人： Frank Bachmann , Hans J. Kutzner , Hans Sonnen , Georg Thierbach , Petra-Sabine Kautz , Alfred Puhler , Andreas Schaefer
发明人： Frank Bachmann , Hans J. Kutzner , Hans Sonnen , Georg Thierbach , Petra-Sabine Kautz , Alfred Puhler , Andreas Schaefer
IPC分类号： C12N1/21 , C12N15/09 , C12N15/77 , C12P13/08 , C12R1/15
CPC分类号： C12P13/08 , C12N15/77 , Y10S435/843
摘要： The present invention relates to new plasmids isolated from Corynebacterium glutamicum, plasmid pGA1 and pGA2, which are compatible with one another. In addition, the present invention relates to recombinant plasmids and to plasmid vectors (shuttle vectors) containing the new plasmids. The plasmids and plasmid vectors of the present invention are suitable for use with genetic engineering to improve bacteria strains.
摘要翻译：本发明涉及从谷氨酸棒杆菌分离的新质粒，质粒pGA1和pGA2，其彼此相容。此外，本发明涉及重组质粒和含有新质粒的质粒载体（穿梭载体）。本发明的质粒和质粒载体适用于遗传工程以改善细菌菌株。

24. 发明授权

US4782022A Nitrogen fixation regulator genes 失效
标题翻译：氮固定调节基因
公开(公告)号：US4782022A
公开(公告)日：1988-11-01
申请号：US737029
申请日：1985-05-22
申请人： Alfred Puhler , Helmut Reilaender , Gerhard Weber
发明人： Alfred Puhler , Helmut Reilaender , Gerhard Weber
IPC分类号： C12N15/00 , C07H15/12 , C12N1/20
CPC分类号： C12N15/00
摘要： Isolation and characterization of a gene which activated nitrogen fixation genes of Rhizobium meliloti when that bacterium is in a symbiotic relationship with a plant is disclosed. This newly discovered gene, designated fix D, can activate the nifHD promoter. A method of making this inducible gene constitutive is presented. This is useful for making nifHD constitutive. The combination of the fixD promoter with heterologous structural genes is taught. Such combinations are useful for limiting expression of an encoded protein to rhizobia involved in a symbiotic relationship with a plant. Plasmids and methods useful in performance of this invention are also disclosed.
摘要翻译：公开了当该细菌与植物共生关系时活化根瘤菌根瘤菌的固氮基因的基因的分离和表征。这个新发现的基因，命名为固定物D，可以激活nifHD启动子。提出了一种制作这种诱导型基因本构的方法。这对于制造nifHD本构是有用的。教导了fixD启动子与异源结构基因的组合。这样的组合可用于将编码的蛋白质的表达限制为与植物共生关系参与的根瘤菌。还公开了用于实施本发明的质粒和方法。

25. 发明申请

US20130302855A1 GENOMICS OF ACTINOPLANES UTAHENSIS 有权
标题翻译： ACTINOPLANES UTAHENSIS的基因学
公开(公告)号：US20130302855A1
公开(公告)日：2013-11-14
申请号：US13814235
申请日：2011-08-01
申请人： Klaus Selber , Bernhard Weingaertner , Hermann Wehlmann , Winfried Rosen , Alfred Puhler , Patrick Schwientek , Jörn Kalinowski , Udo Wehmeier
发明人： Klaus Selber , Bernhard Weingaertner , Hermann Wehlmann , Winfried Rosen , Alfred Puhler , Patrick Schwientek , Jörn Kalinowski , Udo Wehmeier
IPC分类号： C12N1/20 , C12P19/26
CPC分类号： C12N1/20 , C07K14/365 , C12P19/26
摘要： The present invention describes the DNA-sequence of the wild type genome as well as all genetic modifications which were introduced into the wild type-and further developed strains, based thereon. Thereby the first genotypic characterization of the developed strains, including the latest production strain, has been accomplished, accounting for the major part of the invention. Furthermore, on the basis of the determined DNA-sequences, potential genes were identified and account, combined with their functional annotation, for another part of the invention. In particular, the gene-and DNA-sequences, as well as protein-sequences derived there out, contribute to the invention which were affected by mutagenic modifications throughout the strain development process, potentially contributing to the increased production yield.
摘要翻译：本发明描述了野生型基因组的DNA序列以及引入到野生型基因中的所有遗传修饰以及进一步开发的菌株。因此，已经完成了开发菌株的第一个基因型表征，包括最新的生产菌株，占本发明的主要部分。此外，在确定的DNA序列的基础上，鉴定潜在的基因并结合其功能注释，与本发明的另一部分相结合。特别地，基因和DNA序列以及从那里导出的蛋白质序列有助于在整个应变发展过程中受诱变修饰影响的本发明，潜在地有助于提高产量。

26. 发明授权

US06184006B2 Method for the fermentative production of D-pantothenic acid using strains of the family enterobacteriaceae 失效
标题翻译：使用肠杆菌科菌株发酵生产D-泛酸的方法
公开(公告)号：US06184006B2
公开(公告)日：2001-02-06
申请号：US09318739
申请日：1999-05-26
申请人： Mechthild Rieping , Georg Thierbach , Walter Pfefferle , Nicole Dusch , Jorn Kalinowski , Alfred Puhler
发明人： Mechthild Rieping , Georg Thierbach , Walter Pfefferle , Nicole Dusch , Jorn Kalinowski , Alfred Puhler
IPC分类号： C12P1300
CPC分类号： C12N15/52 , C12N9/88 , C12P13/02
摘要： A method for the production of D-pantothenic acid by the fermentation of microorganisms of the family Enterobacteriacae producing D-pantothenic acid which is characterized in that strains are used which a) Contain the plasmid pFV31 and/or pFV202 and that b) A panD gene and optionally other nucleotide sequences coding for aspartate-1-decarboxylase are enhanced, especially overexpressed in these microorganisms, c) The pantothenic acid is enriched in the medium or in the cells of the microorganisms and d) The pantothenic acid formed is isolated.
摘要翻译：通过发酵产生D-泛酸家族肠杆菌的方法生产D-泛酸的方法，其特征在于使用以下菌株：a）含有质粒pFV31和/或pFV202，以及b）PanD基因和任选地编码天冬氨酸-1-脱羧酶的其他核苷酸序列增强，特别是在这些微生物中过表达，c）泛酸在培养基中或微生物细胞中富集，d）分离形成的泛酸。

27. 发明授权

US5516669A Transposon consisting of a fragment of the R plasmid pCxM82B, test vectors containing this transposon, and methods of mutagenesis 失效
标题翻译：由R质粒pCxM82B的片段组成的转座子，含有该转座子的测试载体和诱变方法
公开(公告)号：US5516669A
公开(公告)日：1996-05-14
申请号：US181164
申请日：1994-01-13
申请人： Friedrich Kassing , Andreas Schafer , Jorn Kalinowski , Alfred Puhler
发明人： Friedrich Kassing , Andreas Schafer , Jorn Kalinowski , Alfred Puhler
IPC分类号： C12N1/21 , C12N15/09 , C12N15/70 , C12N15/74 , C12N15/77 , C12R1/19 , C12N15/52 , C12N15/63
CPC分类号： C12N15/77 , C12N15/74
摘要： The invention relates to a transposon consisting of a fragment of the R plasmid pCxM82B from Corynebacterium xerosis DSM 5021, to mobilizable, non-self-transferrable vectors which contain this transposon, and to methods for the mutagenesis of gram-positive bacteria by means of the transfer of these vectors and the generation of auxotrophic mutants.
摘要翻译：本发明涉及一种转座子，其由来自棒状杆菌DSM 5021的R质粒pCxM82B的片段组成，含有该转座子的可移动的非自身转移载体，以及通过以下方法诱变革兰氏阳性细菌的方法：这些载体的转移和营养缺陷突变体的产生。

28. 发明授权

US4686184A Gene transfer vector 失效
标题翻译：基因转移载体
公开(公告)号：US4686184A
公开(公告)日：1987-08-11
申请号：US510296
申请日：1983-07-01
申请人： Alfred Puhler , Reinhard Simon
发明人： Alfred Puhler , Reinhard Simon
IPC分类号： C12N15/74 , C12N15/00 , C12N1/00 , C12N1/20 , C12R1/41
CPC分类号： C12N15/743 , Y10S435/878
摘要： A Tn-Mob element is provided comprising a transposon with a Mob-site inserted therein. Vectors and bacterial strains are provided containing the Tn-Mob element, as well as methods for its use. Such methods include the production of plasmid free bacterial strains.
摘要翻译：提供Tn-Mob元件，其包含其中插入有Mob位点的转座子。提供含有Tn-Mob元件的载体和细菌菌株，以及其使用方法。这样的方法包括产生无质粒细菌菌株。

29. 发明申请

US20100190653A1 Nucleotide sequences encoding alanine racemase from coryneform 有权
标题翻译：从棒状杆菌编码丙氨酸消旋酶的核苷酸序列
公开(公告)号：US20100190653A1
公开(公告)日：2010-07-29
申请号：US10304743
申请日：2002-11-27
申请人： Andreas Tauch , Michael Binder , Walter Pfefferle , Georg Thierbach , Jorn Kalinowski , Alfred Puhler
发明人： Andreas Tauch , Michael Binder , Walter Pfefferle , Georg Thierbach , Jorn Kalinowski , Alfred Puhler
IPC分类号： C40B30/04 , C07H21/04 , C12N1/20 , C12N1/21 , C12N15/74 , C12P13/04 , C12P13/06 , C12P13/08
CPC分类号： C12N9/90 , C12P13/06 , C12P13/08 , C12Y501/01001
摘要： The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the alr gene, and a host-vector system having a coryneform host bacterium in which the alr gene is present in attenuated form and a vector which carries at least the alr gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
摘要翻译：本发明涉及具有编码alr基因的多核苷酸序列的分离的多核苷酸，以及具有其中alr基因以减毒形式存在的棒状细胞宿主细菌的宿主 - 载体系统和至少携带alr基因的载体至SEQ ID No 1，以及使用包含根据本发明的序列的多核苷酸作为杂交探针。

30. 发明申请

US20060205046A1 NUCLEOTIDE SEQUENCES WHICH CODE FOR THE ZWA1 GENE 失效
标题翻译：编号为ZWA1基因的核苷酸序列
公开(公告)号：US20060205046A1
公开(公告)日：2006-09-14
申请号：US11419529
申请日：2006-05-22
申请人： Bettina Mockel , Walter Pfefferle , Achim Marx , Jorn Kalinowski , Brigitte Bathe , Alfred Puhler
发明人： Bettina Mockel , Walter Pfefferle , Achim Marx , Jorn Kalinowski , Brigitte Bathe , Alfred Puhler
IPC分类号： C12P13/08 , C12N15/74 , C12N1/21
CPC分类号： C07K14/34 , C12N9/00 , C12P13/08
摘要： Disclosed herein is an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID NO:2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID NO:2 c) polynucleotide which is complementary to the polynucleotides of a) and b) and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acid with amplification of the zwa1 gene in the coryneform bacteria employed.
摘要翻译：本文公开了一种分离的多核苷酸，其包含选自以下的多核苷酸序列：a）与编码包含SEQ ID NO：2的氨基酸序列的多肽的多核苷酸的程度相同的多核苷酸 b）编码多肽的多核苷酸，其包含与SEQ ID NO：2的氨基酸序列至少70％相同程度的氨基酸序列c）与a）和 b）和d）包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及在所用棒状细菌中扩增zwa1基因的L-氨基酸的发酵制剂的方法。

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