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    • 22. 发明授权
    • Cryopreserving and cryogenically processing biological objects
    • 冷冻保存和低温处理生物体
    • US5925511A
    • 1999-07-20
    • US836092
    • 1997-08-29
    • Gunter FuhrJan HornungRolf HagedornTorsten MullerSteffen HowitzBernd WagnerUlrich Hofmann
    • Gunter FuhrJan HornungRolf HagedornTorsten MullerSteffen HowitzBernd WagnerUlrich Hofmann
    • A01N1/00A01N1/02A01N3/00
    • A01N1/02A01N1/0257A01N1/0278A01N1/0294
    • For the fixated cryopreservation of single living biological objects or s objects compiled in a given number (for example cells) a cryogenically cooled substrate (13) is jetted therewith in a enveloping solution in microdroplet form (12) from a storage vessel, for example by means of a microdroplet jetting device (11). The substrate is temperature-controlled via a coolant (15), the surface to be jetted being located in a gas atmosphere or in vacuum (14). The substrate surface is maintained at a temperature T1 resulting in freezing of the impinging microdroplet, the substrate surface being possibly supportingly microstructured and comprising sensing elements. By controlled movement of either the substrate or the microdroplet jetting device the microdroplets can be applied singly and in patterns in arrays freely selectable or predetermined by the structuring of the substrate. The substrate with the applied microdroplets and the cells frozen therein are stored and/or processed by a processing means (17) ablating the material or applying said material at temperatures T2 of down to -273.degree. C. In the cryogenic condition manipulations such as removal by mechanical ablation or addition of solutions or substances to the surface of the substrate can be undertaken. During thawing the substrate surface is brought to a temperature T1 above the freezing point of the enveloping solution and the microdroplet array thawed in a predefined manner.
    • PCT No.PCT / DE95 / 01490 Sec。 371日期1997年8月29日 102(e)日期1997年8月29日PCT提交1995年10月26日PCT公布。 第WO96 / 13159号公报 日期1996年5月9日对于以一定数量(例如细胞)编制的单一活生物体或此类物体的固定式低温保存,将低温冷却的基底(13)以微滴形式(12)的包封溶液从储存容器 ,例如通过微滴喷射装置(11)。 衬底通过冷却剂(15)进行温度控制,待喷射的表面位于气体气氛或真空中(14)。 衬底表面保持在温度T1,导致撞击微滴的冻结,衬底表面可能被支撑地微结构化并且包括感测元件。 通过基板或微滴喷射装置的受控运动,微滴可以单独施加,并且可以通过基板的结构自由选择或预定的阵列中的图案施加。 具有施加的微滴和在其中冷冻的细胞的基质通过处理装置(17)储存和/或处理,该处理装置(17)在低至-273℃的温度T2下烧蚀材料或施加所述材料。在低温条件下操作,例如去除 可以通过机械消融或将溶液或物质添加到基材表面进行。 在解冻期间,将衬底表面的温度T1提高到高于包络溶液的凝固点并且微滴阵列以预定方式解冻。
    • 25. 发明授权
    • Method and device for aligning and storing in a given order moldings
having a tubular perforation
    • 用于以给定的顺序对准和存储具有管状穿孔的模制品的方法和装置
    • US4995779A
    • 1991-02-26
    • US302164
    • 1989-01-25
    • Torsten Muller
    • Torsten Muller
    • B23Q7/18B65G47/14B65G47/256
    • B65G47/256
    • "Method and device for aligning and storing in a given order moldings having a tubular perforation."Individual moldings (6) are introduced successively from above into a turbulence space above a receiving mandrel (5) profiled for reception in the perforation (7) in the molding (6). Each molding is subjected to an aligning air turbulence until the molding (6) has been aligned with its perforation (7) with respect to the receiving mandrel (5) in such a manner that the molding (6) falls onto the receiving mandrel (5) due to an air resistance decreasing as a result of the alignment. The receiving mandrel (5) then passes the molding (6) on to a deposition transport rail (19).
    • “用于以给定的顺序对准和存储具有管状穿孔的模制品的方法和装置”。将各个模制件(6)从上方连续地引入到接收心轴(5)上方的湍流空间中,该容纳心轴(5)被成形为用于在穿孔(7)中接收 成型(6)。 每个模制件经受对准的空气湍流,直到模制件(6)相对于接收心轴(5)已经与其穿孔(7)对准,使得模制件(6)落在接收心轴(5)上 )由于对准的结果导致空气阻力降低。 接收心轴(5)然后将模制件(6)传递到沉积输送轨道(19)上。