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21. 发明授权

US08877449B2 Method for obtaining pancreatic progenitor cell using NEPH3 有权
标题翻译：使用NEPH3获得胰腺祖细胞的方法
公开(公告)号：US08877449B2
公开(公告)日：2014-11-04
申请号：US13141063
申请日：2009-12-18
申请人： Yuichi Ono , Tomoya Nakatani , Yasuko Nakagawa
发明人： Yuichi Ono , Tomoya Nakatani , Yasuko Nakagawa
IPC分类号： G01N33/53 , G01N33/68 , C07K14/47 , C12N5/071
CPC分类号： C12N5/0678
摘要： The present invention provides markers that can selectively distinguish pancreatic progenitor cells. The present invention also provides methods for distinguishing pancreatic progenitor cells by using the markers as an indicator, and reagents to be used in the methods.The present inventors successfully identified the surface marker Nephrin-like 3 (Neph3) which is specifically expressed in pancreatic progenitor cells, and isolated pancreatic progenitor cells using the marker as an indicator or such. Viable pancreatic progenitor cells can be selected by using Neph3 as an indicator without using translated products and transcripts of any foreign genes. The marker is useful in preparing and identifying pancreatic progenitor cells which are applied to regenerative medicine or such for treatment of pancreatic diseases.
摘要翻译：本发明提供了可以选择性区分胰腺祖细胞的标志物。本发明还提供了通过使用标记作为指标来区分胰腺祖细胞的方法，以及用于该方法的试剂。本发明人成功地鉴定了在胰腺祖细胞中特异性表达的表面标记物Nephrin样3（Neph3），以及使用该标记作为指示剂的分离的胰腺祖细胞。可以通过使用Neph3作为指标来选择可行的胰腺祖细胞，而不使用翻译的产物和任何外来基因的转录物。该标记物可用于制备和鉴定适用于再生医学或胰腺疾病治疗的胰腺祖细胞。

22. 发明授权

US08580523B2 Specific marker Lmx1a on dopaminergic neurons 有权
标题翻译：多巴胺能神经元上的特异性标记物Lmx1a
公开(公告)号：US08580523B2
公开(公告)日：2013-11-12
申请号：US13352241
申请日：2012-01-17
申请人： Yuichi Ono , Yasuko Nakagawa , Tomoya Nakatani
发明人： Yuichi Ono , Yasuko Nakagawa , Tomoya Nakatani
IPC分类号： C12N5/0793 , C12N5/071 , C12N5/00 , C12N5/07 , C12Q1/68 , G01N33/53 , G01N33/58
CPC分类号： C12Q1/6881 , C12Q1/6883 , C12Q2600/158 , G01N33/6896 , G01N33/9413 , G01N2800/2835
摘要： The present invention identified Lmx1a genes, which are expressed in dopaminergic neurons at all differentiation stages, from proliferating dopaminergic neuron progenitor cells before cell cycle exit to cells after cell cycle exit. Lmx1a expression in cells can be used as an indicator when selecting cells suitable for transplantation therapy for neurodegenerative diseases such as Parkinson's disease, and is useful as a marker for screening agents involved in the induction of dopaminergic neuron differentiation.
摘要翻译：本发明鉴定了在细胞周期退出至细胞周期后出现的细胞周期性增殖的多巴胺能神经元祖细胞中，在所有分化阶段的多巴胺能神经元中表达的Lmx1a基因。当选择适合于帕金森病等神经变性疾病的移植治疗的细胞时，细胞中的Lmx1a表达可用作指示剂，并且可用作参与多巴胺能神经元分化诱导的筛选剂的标记。

23. 发明授权

US08067161B2 Dopaminergic neuron proliferative progenitor cell marker Nato3 失效
标题翻译：多巴胺能神经元增殖祖细胞标记Nato3
公开(公告)号：US08067161B2
公开(公告)日：2011-11-29
申请号：US12064018
申请日：2006-08-18
申请人： Yuichi Ono , Yasuko Nakagawa , Tomoya Nakatani , Yoshimasa Sakamoto
发明人： Yuichi Ono , Yasuko Nakagawa , Tomoya Nakatani , Yoshimasa Sakamoto
IPC分类号： C07H21/04 , C12Q1/68
CPC分类号： C07K16/18 , C07K14/47 , C12Q1/6881 , C12Q2600/158 , G01N33/5073
摘要： The present invention is a probe, a primer, and an antibody, for detecting a dopaminergic neuron proliferative progenitor cell. According to the present invention, there is provided a polynucleotide probe and a polynucleotide primer for use in the detection or selection of a dopaminergic neuron proliferative progenitor cell, which can hybridize with a polynucleotide consisting of a nucleotide sequence of a Nato3 gene, or a complementary sequence thereto, and an antibody against a Nato3 protein, or a part thereof for use in the detection or selection of a dopaminergic neuron proliferative progenitor cell.
摘要翻译：本发明是用于检测多巴胺能神经元增殖祖细胞的探针，引物和抗体。根据本发明，提供了用于检测或选择多巴胺能神经元增殖祖细胞的多核苷酸探针和多核苷酸引物，其可与由Nato3基因的核苷酸序列或互补的多核苷酸组成的多核苷酸杂交序列，以及针对Nato3蛋白或其部分的抗体用于检测或选择多巴胺能神经元增殖祖细胞。

24. 发明申请

US20100323366A1 Methods of Selecting a Dopaminergic Neuron Proliferative Progenitor Cells Using LRP4/CORIN Markers 有权
标题翻译：使用LRP4 / CORIN标记物选择多巴胺能神经元增殖祖细胞的方法
公开(公告)号：US20100323366A1
公开(公告)日：2010-12-23
申请号：US12868598
申请日：2010-08-25
申请人： Yuichi Ono , Yasuko Nakagawa , Yoshimasa Sakamoto
发明人： Yuichi Ono , Yasuko Nakagawa , Yoshimasa Sakamoto
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6883 , C12Q2600/158
摘要： In neuron transplantation therapy, in terms of safety, it is preferable to use a cell population consisting only of a desired type of cells, and to use postmitotic neurons in consideration to avoid the risk of tumorigenesis. Moreover, greater therapeutic effects would be expected through the use of earlier progenitor cells in consideration of post-transplantation viability, proper network formation ability, and such.According to the present invention, Lrp4, a gene that is specifically expressed in dopaminergic neuron proliferative progenitor cells prior to cell cycle exit, was identified. The use of Lrp4 expression in cells as an index allows for the isolation. of cells suitable for transplantation therapy of neurodegenerative diseases such as Parkinson's disease in terms of safety, survival rate, and network formation ability.
摘要翻译：在神经元移植治疗中，在安全性方面，优选使用仅由期望类型的细胞组成的细胞群，并考虑使用神经元神经元以避免肿瘤发生的风险。此外，考虑到移植后的生存力，适当的网络形成能力等，通过使用较早的祖细胞可以预期更大的治疗效果。根据本发明，鉴定了在细胞周期退出之前在多巴胺能神经元增殖祖细胞中特异性表达的基因Lrp4。在细胞中使用Lrp4表达作为指标，可以进行分离。的细胞在安全性，存活率和网络形成能力方面适用于帕金森病等神经变性疾病的移植治疗。

25. 发明授权

US07824676B2 Exocrine gland tight junction-constituting protein jeap family 失效
标题翻译：外分泌腺紧密连接蛋白家族
公开(公告)号：US07824676B2
公开(公告)日：2010-11-02
申请号：US11522043
申请日：2006-09-15
申请人： Miyuki Nishimura , Mayumi Asano , Yuichi Ono , Koji Morimoto , Masakazu Takeuchi , Yoko Inoue , Toshio Imai , Yoshimi Takai
发明人： Miyuki Nishimura , Mayumi Asano , Yuichi Ono , Koji Morimoto , Masakazu Takeuchi , Yoko Inoue , Toshio Imai , Yoshimi Takai
IPC分类号： C07K16/18
CPC分类号： C07K14/47 , C07K14/705
摘要： A mouse cDNA library from gene fragments encoding proteins localizing at cell-cell junctions was screened by a technique visualizing localization of a protein to identify a junction-enriched and -associated protein, JEAP. GenBank homology search was performed based on the sequence. Based on the obtained sequence, a mouse cDNA library was screened to identify JEAP-2. By using prepared antibodies against these proteins, it was revealed that these proteins express specifically at tight junctions, in particular, tight junctions in exocrine glands.
摘要翻译：通过技术可视化蛋白质的定位技术筛选编码在细胞连接处定位的蛋白质的蛋白质的基因片段的小鼠cDNA文库，以鉴定连接蛋白和相关蛋白JEAP。 GenBank同源性检索是根据序列进行的。基于获得的序列，筛选小鼠cDNA文库以鉴定JEAP-2。通过使用针对这些蛋白质的制备的抗体，显示这些蛋白质在紧密连接处特别是在外分泌腺中的紧密连接处特异性表达。

26. 发明申请

US20100203505A1 DOPAMINERGIC NEURON PROLIFERATIVE PROGENITOR CELL MARKER Msx1/2 审中-公开
标题翻译：多巴胺神经元增殖性前体细胞标记Msx1 / 2
公开(公告)号：US20100203505A1
公开(公告)日：2010-08-12
申请号：US12064019
申请日：2006-08-18
申请人： Yuichi Ono , Yasuko Nakagawa , Tomoya Nakatani , Yoshimasa Sakamoto
发明人： Yuichi Ono , Yasuko Nakagawa , Tomoya Nakatani , Yoshimasa Sakamoto
IPC分类号： C12Q1/68 , C07H21/04 , C07K16/00
CPC分类号： G01N33/56966 , C07K16/18 , C12Q1/6881 , C12Q2600/158
摘要： The present invention is a probe, a primer, and an antibody, for detecting a dopaminergic neuron proliferative progenitor cell. According to the present invention, there is provided a polynucleotide probe and a polynucleotide primer for use in the detection or selection of a dopaminergic neuron proliferative progenitor cell, which can hybridize with a polynucleotide consisting of a nucleotide sequence of an Msx1 gene or an Msx2 gene, or a complementary sequence thereto, and an antibody against an Msx1 protein or an Msx2 protein, or a part thereof for use in the detection or selection of a dopaminergic neuron proliferative progenitor cell.
摘要翻译：本发明是用于检测多巴胺能神经元增殖祖细胞的探针，引物和抗体。根据本发明，提供了用于检测或选择多巴胺能神经元增殖祖细胞的多核苷酸探针和多核苷酸引物，其可以与由Msx1基因或Msx2基因的核苷酸序列组成的多核苷酸杂交，或其互补序列，以及针对用于检测或选择多巴胺能神经元增殖祖细胞的Msx1蛋白或Msx2蛋白或其一部分的抗体。

27. 发明申请

US20060240432A1 Lrp4/corin dopamine-producing neuron proliferation precursor cell marker 审中-公开
标题翻译： Lrp4 / corin多巴胺产生神经元增殖前体细胞标记
公开(公告)号：US20060240432A1
公开(公告)日：2006-10-26
申请号：US10543003
申请日：2004-01-23
申请人： Yuichi Ono , Yasuko Nakagawa , Yoshimasa Sakamoto
发明人： Yuichi Ono , Yasuko Nakagawa , Yoshimasa Sakamoto
IPC分类号： C12Q1/68 , C12N5/08
CPC分类号： C12Q1/6883 , C12Q2600/158
摘要： In neuron transplantation therapy, in terms of safety, it is preferable to use a cell population consisting only of a desired type of cells, and to use postmitotic neurons in consideration to avoid the risk of tumorigenesis. Moreover, greater therapeutic effects would be expected through the use of earlier progenitor cells in consideration of post-transplantation viability, proper network formation ability, and such. According to the present invention, Lrp4, a gene that is specifically expressed in dopaminergic neuron proliferative progenitor cells prior to cell cycle exit, was identified. The use of Lrp4 expression in cells as an index allows for the isolation. of cells suitable for transplantation therapy of neurodegenerative diseases such as Parkinson's disease in terms of safety, survival rate, and network formation ability.
摘要翻译：在神经元移植治疗中，在安全性方面，优选使用仅由期望类型的细胞组成的细胞群，并考虑使用神经元神经元以避免肿瘤发生的风险。此外，考虑到移植后的生存力，适当的网络形成能力等，通过使用较早的祖细胞可以预期更大的治疗效果。根据本发明，鉴定了在细胞周期退出之前在多巴胺能神经元增殖祖细胞中特异性表达的基因Lrp4。在细胞中使用Lrp4表达作为指标，可以进行分离。的细胞在安全性，存活率和网络形成能力方面适用于帕金森病等神经变性疾病的移植治疗。

28. 发明授权

US06325434B1 Tongs apparatus equipped with supplemental-holding-force apparatus 有权
标题翻译：钳子装备配备补充力装置
公开(公告)号：US06325434B1
公开(公告)日：2001-12-04
申请号：US09590292
申请日：2000-06-09
申请人： Seiyu Takao , Yuichi Ono , Kazuhiro Tanaka , Kazuo Izumi
发明人： Seiyu Takao , Yuichi Ono , Kazuhiro Tanaka , Kazuo Izumi
IPC分类号： B66C142
CPC分类号： B66C1/422 , B66C1/425
摘要： A tongs apparatus includes an upper frame suspended from a crane, a pair of first arms journaled on the upper frame, a pair of second arms journaled on the first arms and disposed to cross each other, and a supplemental-holding-force apparatus. The supplemental-holding-force apparatus comprises a chain motor; a chain wound and unwound by the chain motor; a ring-shaped member connected to the chain and having an elongated hole; and a lifting metal fitting having a guide pin slidably engaged with the ring-shaped member. The chain is wound or unwound by the chain motor in order to move the ring-shaped member vertically to thereby supplement a holding force generated through closing of the second arms.
摘要翻译：夹钳装置包括从起重机悬挂的上框架，在上框架上轴颈的一对第一臂，一对第二臂，其位于第一臂上并彼此交叉设置，以及辅助保持力装置。补充保持力装置包括链式电动机; 由链条电机缠绕并展开的链条; 连接到链条并具有细长孔的环形构件; 以及具有与所述环形构件可滑动地接合的引导销的起重金属配件。链条由链式电动机缠绕或退绕，以便垂直移动环形构件，从而补充通过关闭第二臂产生的保持力。

29. 发明授权

US5283846A Optical isolator, optical circuit, and rare-earth-doped fiber optical amplifier 失效
标题翻译：光隔离器，光电路和稀土掺杂光纤放大器
公开(公告)号：US5283846A
公开(公告)日：1994-02-01
申请号：US898556
申请日：1992-06-15
申请人： Takashi Toyonaka , Shinji Tsuji , Makoto Haneda , Yuichi Ono
发明人： Takashi Toyonaka , Shinji Tsuji , Makoto Haneda , Yuichi Ono
IPC分类号： G02B27/28 , G02B6/42 , G02F1/09 , G02F1/35 , H01S3/06 , H01S3/067 , H01S3/07 , H01S3/10 , G02B6/32
CPC分类号： H01S3/06754 , G02B6/4208 , G02F1/093 , G02F2203/06 , Y10S372/703
摘要： In a polarization dependency type optical isolator comprising wedgelike polarizing prisms 3 and 5 and a Faraday rotator 4, the mode field diameters of an input side fiber 1 and an output side fiber 7 are the same as that of a transmission fiber and an erbium-doped fiber to be connected to them. The shape and other features of each lens are controlled so that the spot size of a parallel ray which is converted from an incident ray by an input side lens 2 is equal to the spot size of a parallel ray which is converted by an output side lens 6 when a reference beam is irradiated from the fiber 7. In a rare-earth-doped fiber optical amplifier using an optical isolator which is configured in a manner similar to this, the connecting portion of the fiber and the optical isolator can be spliced by fusion without increasing the insertion loss of the optical isolator in the forward direction and the connection loss due to a difference in the mode field diameter is reduced. Therefore, the noise figure decreases and the gain increases.
摘要翻译：在包括楔形偏振棱镜3和5以及法拉第旋转器4的偏振依赖型光隔离器中，输入侧光纤1和输出侧光纤7的模场直径与传输光纤和掺铒光纤的模场直径相同光纤连接到它们。控制每个透镜的形状和其它特征，使得通过输入侧透镜2从入射光线转换的平行光线的光斑尺寸等于由输出侧透镜转换的平行光线的光斑尺寸 6，当从光纤7照射参考光束时。在采用与此类似的方式配置的光隔离器的稀土掺杂光纤放大器中，光纤和光隔离器的连接部分可以被融合而不增加光隔离器在正向上的插入损耗，并且由于模场直径差导致的连接损耗减小。因此，噪声系数下降，增益增加。

30. 发明授权

US5139125A Temperature sensitive type fluid fan coupling apparatus 失效
标题翻译：温度敏感型流体传感器联轴器
公开(公告)号：US5139125A
公开(公告)日：1992-08-18
申请号：US718024
申请日：1991-06-20
申请人： Kazunori Takikawa , Yuichi Ono , Hiroshi Inoue
发明人： Kazunori Takikawa , Yuichi Ono , Hiroshi Inoue
IPC分类号： F01P7/08 , F16D35/00 , F16D35/02
CPC分类号： F16D35/023
摘要： Herein disclosed is a temperature sensitive fluid type fan coupling apparatus which comprises: a sealed container including a casing and a cover, a partition dividing the inside of the sealed container into an oil sump and a torque transmission chamber housing the drive disc; a dam formed in a portion of such an inner circumference of the sealed container as faces the outer circumference of the drive disc, on which the oil is collected at the time of rotation; a circulation passage communicating with the dam and leading from the torque transmission chamber to the oil sump; a valve member fitted in the oil sump for opening an outflow adjusting orifice leading to the torque transmission chamber, if the ambient temperature exceeds a preset value, and closing the same if below the preset value; and a spiral temperature sensing element mounted on the front face of the cover and associated with the valve member if spirally deformed in accordance with the temperature change. The effective contact area of the oil at a torque transmission gap is increased or decreased to control the torque transmission from the rotating shaft at the drive side to the sealed container at the driven side. The output adjusting orifice is formed through a portion of the inner circumference of the cover forming the oil sump. A sealing portion for sliding in the circumferential directions to open or close the outflow adjusting orifice is formed at the leading end of the valve member.
摘要翻译：这里公开了一种温度敏感型的风扇联接装置，其包括：密封容器，其包括壳体和盖子，将密封容器内部分成油底壳的分隔件和容纳驱动盘的扭矩传递室; 形成在所述密封容器的所述内圆周的面向所述驱动盘的外周的部分，所述驱动盘的外周在旋转时收集油; 与坝连通并从扭矩传递室引导到油底壳的循环通道; 安装在油底壳中的阀构件，用于打开通向扭矩传递室的流出调节孔，如果环境温度超过预设值，并且如果低于预设值则将其关闭; 以及安装在所述盖的前表面上且与所述阀构件相关联的螺旋温度感测元件，如果根据所述温度变化而螺旋形变形。在转矩传递间隙处的油的有效接触面积被增大或减小，以控制从驱动侧的旋转轴到从动侧的密封容器的转矩传递。输出调节孔通过形成油底壳的盖的内周的一部分形成。在阀构件的前端形成有用于在圆周方向上滑动以打开或关闭流出调节孔的密封部。

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