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21. 发明授权

US07399852B2 Kits and reaction mixtures containing modified probe molecules 有权
标题翻译：试剂盒和含有修饰的探针分子的反应混合物
公开(公告)号：US07399852B2
公开(公告)日：2008-07-15
申请号：US09808558
申请日：2001-03-14
申请人： Michael M. Becker , Mehrdad Majlessi , Steven T. Brentano
发明人： Michael M. Becker , Mehrdad Majlessi , Steven T. Brentano
IPC分类号： C07H21/04
CPC分类号： C12Q1/6832 , C07H21/00 , C12Q1/6816 , C12Q1/6844 , C12Q1/686 , C12Q1/6865 , C12Q2563/131 , C12Q2563/113 , C12Q2525/101 , C12Q2525/117 , C12Q2527/107
摘要： The present invention concerns oligonucleotides containing one or more modified nucleotides which increase the binding affinity of the oligonucleotides to target nucleic acids having a complementary nucleotide base sequence. These modified oligonucleotides hybridize to the target sequence at a faster rate than unmodified oligonucleotides having an identical nucleotide base sequence. Such modified oligonucleotides include oligonucleotides containing at least one 2′-O-methylribofuranosyl moiety joined to a nitrogenous base. Oligonucleotides can be modified in accordance with the present invention to preferentially bind RNA targets. The present invention also concerns methods of using these modified oligonucleotides and kits containing the same.
摘要翻译：本发明涉及含有一个或多个修饰的核苷酸的寡核苷酸，其增加寡核苷酸与具有互补核苷酸碱基序列的靶核酸的结合亲和力。这些修饰的寡核苷酸以比具有相同核苷酸碱基序列的未修饰寡核苷酸更快的速率与靶序列杂交。这种修饰的寡核苷酸包括含有至少一个连接到含氮碱基的2'-O-甲基呋喃核糖基部分的寡核苷酸。可以根据本发明修饰寡核苷酸以优先结合RNA靶。本发明还涉及使用这些修饰的寡核苷酸的方法和含有该寡核苷酸的试剂盒。

22. 发明授权

US6130038A Method for amplifying target nucleic acids using modified primers 失效
标题翻译：使用改良引物扩增靶核酸的方法
公开(公告)号：US6130038A
公开(公告)日：2000-10-10
申请号：US893300
申请日：1997-07-15
申请人： Michael M. Becker , Mehrdad Majlessi , Steven T. Brentano
发明人： Michael M. Becker , Mehrdad Majlessi , Steven T. Brentano
IPC分类号： C12N15/09 , C07H21/00 , C12Q1/68 , C07H21/04
CPC分类号： C07H21/00 , C12Q1/6832 , C12Q1/686 , C12Q1/6865
摘要： The present invention concerns oligonucleotides containing one or more modified nucleotides which increase the binding affinity of the oligonucleotides to target nucleic acids having a complementary nucleotide base sequence. These modified oligonucleotides hybridize to the target sequence at a faster rate than unmodified oligonucleotides having an identical nucleotide base sequence. Such modified oligonucleotides include oligonucleotides containing at least one 2'-O-methylribofuranosyl moiety joined to a nitrogenous base. Oligonucleotides can be modified in accordance with the present invention to preferentially bind RNA targets. The present invention also concerns methods of using these modified oligonucleotides and kits containing the same.
摘要翻译：本发明涉及含有一个或多个修饰的核苷酸的寡核苷酸，其增加寡核苷酸与具有互补核苷酸碱基序列的靶核酸的结合亲和力。这些修饰的寡核苷酸以比具有相同核苷酸碱基序列的未修饰寡核苷酸更快的速率与靶序列杂交。这种修饰的寡核苷酸包括含有至少一个连接到含氮碱基的2'-O-甲基呋喃核糖基部分的寡核苷酸。可以根据本发明修饰寡核苷酸以优先结合RNA靶。本发明还涉及使用这些修饰的寡核苷酸的方法和含有该寡核苷酸的试剂盒。

23. 发明授权

US07326533B2 Detection rpoB sequences of Mycobacterium tuberculosis 有权
标题翻译：检测结核分枝杆菌的rpoB序列
公开(公告)号：US07326533B2
公开(公告)日：2008-02-05
申请号：US11495262
申请日：2006-07-27
申请人： Markus T. Jucker , Steven T. Brentano , Francisco D. Delgado , Philippe Cleuziat
发明人： Markus T. Jucker , Steven T. Brentano , Francisco D. Delgado , Philippe Cleuziat
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/689 , C12Q2600/156
摘要： A method of detecting rpoB sequences of Mycobacterium tuberculosis present in a biological sample that includes steps of amplifying the M. tuberculosis rpoB sequence in vitro in a nucleic acid amplification mixture that includes specific disclosed primer sequences, and detecting the amplified sequences by using probes that provide information by their specific hybridization to portions of the amplified nucleic acid is disclosed. Compositions for amplifying and detecting in vitro the rpoB sequences of M. tuberculosis in a sample are disclosed.
摘要翻译：一种检测存在于生物样品中的结核分枝杆菌的rpoB序列的方法，其包括在包括特异性公开的引物序列的核酸扩增混合物中体外扩增结核分枝杆菌rpoB序列的步骤，并通过使用提供的探针检测扩增的序列公开了通过其与部分扩增核酸的特异性杂交的信息。公开了用于在体外扩增和检测样品中结核分枝杆菌的rpoB序列的组合物。

24. 发明授权

US07094542B2 Detection of rpoB sequences of Mycobacterium tuberculosis 有权
公开(公告)号：US07094542B2
公开(公告)日：2006-08-22
申请号：US10245988
申请日：2002-09-18
申请人： Markus T. Jucker , Steven T. Brentano , Francisco D. Delgado , Philippe Cleuziat
发明人： Markus T. Jucker , Steven T. Brentano , Francisco D. Delgado , Philippe Cleuziat
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/689 , C12Q2600/156
摘要： A method of detecting rpoB sequences of Mycobacterium tuberculosis present in a biological sample that includes steps of amplifying the M. tuberculosis rpoB sequence in vitro in a nucleic acid amplification mixture that includes specific disclosed primer sequences, and detecting the amplified sequences using multiple probes that provide sequence information by their specific hybridization to portions of the amplified nucleic acid. Compositions for amplifying and detecting in vitro the rpoB sequences of M. tuberculosis in a sample are disclosed.

25. 发明授权

US06297365B1 Decoy probes 有权
标题翻译：诱饵探针
公开(公告)号：US06297365B1
公开(公告)日：2001-10-02
申请号：US09365121
申请日：1999-07-30
申请人： Christopher C. Adams , Steven T. Brentano , Gary P. Schroth
发明人： Christopher C. Adams , Steven T. Brentano , Gary P. Schroth
IPC分类号： C07H2102
CPC分类号： C12Q1/6865 , C07H21/00 , C12Q1/6806 , C12Q2525/301 , C12Q2549/125
摘要： The present invention features inhibitors of target-independent amplification and the use of such inhibitors for enhancing an amplification protocol. The inhibitors are believed to enhance an amplification protocol by inhibiting the ability of one or more nucleic acid polymerases to use nucleic acid in a polymerase reaction in the absence of target nucleic acid.
摘要翻译：本发明的特征在于靶依赖性扩增的抑制剂以及这种抑制剂用于增强扩增方案的用途。认为抑制剂通过抑制一种或多种核酸聚合酶在不存在靶核酸的聚合酶反应中使用核酸的能力来增强扩增方案。

26. 发明授权

US06218107B1 Compositions and methods for detecting the presence of Mycobacterium kansassii 失效
标题翻译：用于检测堪萨斯分枝杆菌存在的组合物和方法
公开(公告)号：US06218107B1
公开(公告)日：2001-04-17
申请号：US08856963
申请日：1997-05-15
申请人： Steven T. Brentano , Irene Andruszkiewicz , Caroline F. Knott
发明人： Steven T. Brentano , Irene Andruszkiewicz , Caroline F. Knott
IPC分类号： C07H2104
CPC分类号： C12Q1/689
摘要： The featured invention discloses and claims oligonucleotide hybridization assay probes and helper oligonucleotides which are designed to be complementary to specific regions of M. kansasii rRNA or the DNA encoding it, or to an oligonucleotide or nucleic acid comprising, consisting essentially of, or consisting of, a M. kansasii rRNA or rDNA nucleotide sequence. The hybridization probes of the present invention are designed to hybridize to a target nucleic acid in a region of the molecule having a specific target nucleotide sequence under conditions which allow the selective detection of the target nucleic acid. The probes are further designed to detect M. kansasii typical as well as atypical strains. The present invention also discloses and claims double-stranded nucleic acid hybrid molecules formed between the hybridization probes and their specific target nucleic acids.
摘要翻译：本发明公开并要求的是寡核苷酸杂交测定探针和辅助寡核苷酸，其被设计为与堪萨斯堪萨斯大学rRNA或编码它的DNA的特定区域互补，或者涉及包含基本上由...组成或由其构成的寡核苷酸或核酸，堪萨斯大肠杆菌rRNA或rDNA核苷酸序列。本发明的杂交探针被设计为在允许选择性检测靶核酸的条件下与具有特异性靶核苷酸序列的分子区域中的靶核酸杂交。探针进一步设计用于检测典型的堪萨斯（Kansasii）典型菌株和非典型菌株。本发明还公开并要求在杂交探针与其特异性靶核酸之间形成的双链核酸杂交分子。

27. 发明授权

US08354226B2 Assay for detection of human parvovirus B19 nucleic acid 有权
公开(公告)号：US08354226B2
公开(公告)日：2013-01-15
申请号：US11459908
申请日：2006-07-25
申请人： Steven T. Brentano , Margarita Batranina-Kaminsky , Cynthia S. Hasselkus-Light , Daniel P. Kolk
发明人： Steven T. Brentano , Margarita Batranina-Kaminsky , Cynthia S. Hasselkus-Light , Daniel P. Kolk
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/701 , C12Q1/6865 , C12Q2525/161 , C12Q2525/113
摘要： Nucleic acid oligomers specific for human parvovirus B19 genomic DNA are disclosed. An assay for amplifying and detecting human parvovirus B19 nucleic acid in biological specimens is disclosed. Compositions for detecting the presence of parvovirus B19 genomic DNA in human biological specimens are disclosed.

28. 发明授权

US07361746B2 Methods and compositions for detection of Mycobacterium avium complex species 有权
标题翻译：用于检测鸟分枝杆菌复合物种的方法和组合物
公开(公告)号：US07361746B2
公开(公告)日：2008-04-22
申请号：US10862026
申请日：2004-06-04
申请人： Steven T. Brentano , Roger L. Lankford
发明人： Steven T. Brentano , Roger L. Lankford
IPC分类号： C07H19/00 , C12Q1/68 , C12P19/34
CPC分类号： C12Q1/689
摘要： Methods of detecting Mycobacterium avium complex (MAC) organisms using in vitro nucleic acid amplification with amplification oligonucleotides specific for 16S rRNA or DNA sequences encoding 16S rRNA from MAC species are disclosed. Compositions and kits containing oligonucleotides for amplifying and detecting 16S rRNA or DNA sequences encoding 16S rRNA from MAC species are disclosed.
摘要翻译：公开了使用具有16S rRNA特异性的扩增寡核苷酸或编码来自MAC物种的16S rRNA的DNA序列的体外核酸扩增来检测鸟分枝杆菌复合物（MAC）生物体的方法。公开了含有用于扩增和检测来自MAC物种的16S rRNA的16S rRNA或DNA序列的寡核苷酸的组合物和试剂盒。

29. 发明授权

US07355034B2 Oligonucleotides for use in determining the presence of human papilloma virus in a test sample 有权
公开(公告)号：US07355034B2
公开(公告)日：2008-04-08
申请号：US10607416
申请日：2003-06-26
申请人： Patricia Gordon , Nick M. Carter , Steven T. Brentano , Philip W. Hammond
发明人： Patricia Gordon , Nick M. Carter , Steven T. Brentano , Philip W. Hammond
IPC分类号： C07H21/02 , C12Q1/68
CPC分类号： C12Q1/708 , C12Q1/6865 , C12Q1/6886 , C12Q2600/112 , C12Q2600/16
摘要： The present invention describes oligonucleotides targeted to HPV Type 16 and/or Type 18 nucleic acid sequences which are particularly useful to aid in detecting HPV type 16 and or 18. The oligonucleotides can aid in detecting HPV Type 16 and/or Type 18 in different ways such as by acting as hybridization assay probes, helper probes, and/or amplification primers.

30. 发明授权

US07294489B2 Nucleic acid amplification and detection of Mycobacterium species 有权
标题翻译：核酸扩增和检测分枝杆菌种
公开(公告)号：US07294489B2
公开(公告)日：2007-11-13
申请号：US10665708
申请日：2003-09-18
申请人： Steven T. Brentano , Markus T. Jucker , Francisco D. Delgado , Philippe Cleuziat , Marc Rodrigue
发明人： Steven T. Brentano , Markus T. Jucker , Francisco D. Delgado , Philippe Cleuziat , Marc Rodrigue
IPC分类号： C12P19/34 , C07H21/04
CPC分类号： C12Q1/689 , C12Q2600/16
摘要： Oligonucleotides used to prime in vitro nucleic acid amplification of 16S rRNA sequences or DNA encoding 16S rRNA sequences for many species within the genus Mycobacterium are disclosed. Kits including such oligonucleotides are disclosed. Methods of detecting Mycobacterium species using the oligonucleotides in in vitro nucleic acid amplification are disclosed.
摘要翻译：公开了用于引发16S rRNA序列的体外核酸扩增的寡核苷酸或编码16S rRNA序列的DNA，用于分枝杆菌属中的许多物种。公开了包括这种寡核苷酸的试剂盒。公开了在体外核酸扩增中使用寡核苷酸检测分枝杆菌物种的方法。

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