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21. 发明申请

US20100129814A1 Method for separation and characterization of microorganisms using identifier agents 有权
标题翻译：使用识别剂分离和表征微生物的方法
公开(公告)号：US20100129814A1
公开(公告)日：2010-05-27
申请号：US12589985
申请日：2009-10-30
申请人： John Walsh , Jones M. Hyman , Thurman Thorpe , Bradford Clay
发明人： John Walsh , Jones M. Hyman , Thurman Thorpe , Bradford Clay
IPC分类号： C12Q1/68 , G01N33/567 , C12Q1/37 , C12Q1/04
CPC分类号： C12Q1/24 , C12Q1/04 , C12Q1/37 , C12Q1/6816 , G01N21/35 , G01N21/3581 , G01N21/47 , G01N21/64 , G01N21/65 , G01N33/6848
摘要： The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides for the use of one or more identifier agents and interrogating the microorganism sample and/or said one or more identifier agents to produce measurements which characterizing and/or identifying the microorganism based on the produced measurements and/or the presence or absence of the identifier agent or a metabolized form of the identifier agent in the microorganism sample.
摘要翻译：本发明涉及一种用于分离，表征和/或鉴定测试样品中的微生物的方法。本发明的方法包括用于裂解可能存在于测试样品中的非微生物细胞的任选裂解步骤，随后是随后的分离步骤。该方法可用于从诸如含血培养基的复杂样品中分离，表征和/或鉴定微生物。本发明进一步提供一种或多种识别剂的使用，并且询问微生物样品和/或所述一种或多种标识物以产生基于产生的测量值和/或存在或不存在来表征和/或鉴定微生物的测量结果的识别剂或微生物样品中识别剂的代谢形式。

22. 发明授权

US07183073B2 Method for detecting microorganisms 有权
标题翻译：微生物检测方法
公开(公告)号：US07183073B2
公开(公告)日：2007-02-27
申请号：US10428655
申请日：2003-05-02
申请人： Jones M. Hyman , Paul M. Matsumura , Scott R. Jeffrey , Martin J. Maresch , Thurman C. Thorpe
发明人： Jones M. Hyman , Paul M. Matsumura , Scott R. Jeffrey , Martin J. Maresch , Thurman C. Thorpe
IPC分类号： C12Q1/02 , C12Q1/04 , C12N11/02 , C12N11/10 , C12N11/08 , C12M1/34
CPC分类号： C12M25/14 , C12Q1/04 , C12Q1/045 , C12Q1/24
摘要： A device and method are provided for isolating and culturing microorganisms from a bulk fluid sample. The device comprises a container having therein a polymeric immobilization layer having interstitial spaces between polymer chains such as a gel matrix. The interstitial spaces are of an average size less than an average size of microorganisms to be separated from the sample and cultured. A bulk fluid sample is applied to the immobilization layer where fluid is absorbed by the layer and microorganisms remain on the surface of the layer. After culturing, microorganism colonies are readily accessible on the surface of the layer for harvest and testing. The immobilization layer may contain one or more of nutrients for microorganisms growth, lytic agents, lytic enzymes, antibiotics, antibiotic neutralizers, indicators, detergents and selective agents. An adjacent support layer may be above and/or below the immobilization layer. The immobilization layer may be in combination with a sensor layer that changes color in areas corresponding to portions of the layer having microorganisms thereon. A membrane may be embedded in the immobilization layer for enhancing microorganism visibility and facilitating microorganism harvest.
摘要翻译：提供了用于从大量流体样品中分离和培养微生物的装置和方法。该装置包括其中具有聚合物固定化层的容器，其在聚合物链之间具有间隙，例如凝胶基质。间隙空间的平均尺寸小于从样品中分离的微生物的平均尺寸并进行培养。将大量流体样品施加到固定层，其中流体被层吸收，微生物保留在层的表面上。培养后，微生物菌落易于在层的表面进行收获和测试。固定层可以含有一种或多种用于微生物生长的营养物，溶解剂，溶解酶，抗生素，抗生素中和剂，指示剂，洗涤剂和选择剂。相邻的支撑层可以在固定层的上方和/或下方。固定层可以与在其上具有微生物层的部分相对应的区域中改变颜色的传感器层组合。膜可以嵌入在固定层中以增强微生物的可见性并促进微生物收获。

23. 发明授权

US08652800B2 Method for separation, characterization and/or identification of microorganisms using spectroscopy 有权
标题翻译：使用光谱法分离，表征和/或鉴定微生物的方法
公开(公告)号：US08652800B2
公开(公告)日：2014-02-18
申请号：US12589952
申请日：2009-10-30
申请人： John Walsh , Jones M. Hyman , Thurman Thorpe , Bradford Clay , Christopher Ronsick
发明人： John Walsh , Jones M. Hyman , Thurman Thorpe , Bradford Clay , Christopher Ronsick
IPC分类号： C12Q1/04
CPC分类号： C12Q1/04 , G01N21/35 , G01N21/3581 , G01N21/359 , G01N21/47 , G01N21/64 , G01N21/65 , G01N33/6848 , Y02A50/55
摘要： The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides for spectroscopic interrogation of the separated microorganism sample to produce measurements of the microorganism and characterizing and/or identifying the microorganism in the sample using said spectroscopic measurements.
摘要翻译：本发明涉及一种用于分离，表征和/或鉴定测试样品中的微生物的方法。本发明的方法包括用于裂解可能存在于测试样品中的非微生物细胞的任选裂解步骤，随后是随后的分离步骤。该方法可用于从诸如含血培养基的复杂样品中分离，表征和/或鉴定微生物。本发明进一步提供分离的微生物样品的光谱询问以产生微生物的测量，并使用所述光谱测量来表征和/或鉴定样品中的微生物。

24. 发明授权

US06777226B2 Sensor device for detecting microorganisms, and method therefor 有权
标题翻译：用于检测微生物的传感器装置及其方法
公开(公告)号：US06777226B2
公开(公告)日：2004-08-17
申请号：US09741402
申请日：2000-12-18
申请人： Scott R. Jeffrey , Paul M. Matsumura , Martin J. Maresch , Jones M. Hyman , Thurman C. Thorpe
发明人： Scott R. Jeffrey , Paul M. Matsumura , Martin J. Maresch , Jones M. Hyman , Thurman C. Thorpe
IPC分类号： C12M134
CPC分类号： C12Q1/04 , C12M23/04 , C12M23/22 , C12M23/24 , C12M25/06 , C12M25/14 , C12Q1/045 , C12Q1/24
摘要： A device and method allow for detecting the presence of microorganisms in clinical and non-clinical specimens. The device, a sensor, provides an environment to culture microbial organism colonies from a fluid sample, and a means to facilitate microbial detection and quantification, either manually or with an instrument. The sensor has a microorganism immobilization matrix layer and a sensor layer. Detected microbial colonies are immediately available for further testing. The sensor provides an area for accepting a fluid sample, a mechanism to immobilize the fluid sample on an interior surface of the plate, nutrients to facilitate growth of microorganisms in the sample, and a sensor for allowing the detection and/or enumeration of microorganism colonies within the sample.
摘要翻译：一种装置和方法允许检测临床和非临床标本中微生物的存在。该装置，传感器，提供了从流体样品培养微生物菌落的环境，以及手动或用仪器促进微生物检测和定量的手段。传感器具有微生物固定基质层和传感器层。检测到的微生物菌落立即可用于进一步测试。传感器提供用于接受流体样品的区域，将流体样品固定在板的内表面上的机制，营养物质以促进样品中微生物的生长，以及用于允许微生物菌落的检测和/或计数的传感器在样品内。

25. 发明授权

US06251624B1 Apparatus and method for detecting, quantifying and characterizing microorganisms 有权
标题翻译：用于检测，定量和表征微生物的装置和方法
公开(公告)号：US06251624B1
公开(公告)日：2001-06-26
申请号：US09502678
申请日：2000-02-11
申请人： Paul M. Matsumura , Jones M. Hyman , Scott R. Jeffrey , Martin J. Maresch , Thurman C. Thorpe , William G. Barron
发明人： Paul M. Matsumura , Jones M. Hyman , Scott R. Jeffrey , Martin J. Maresch , Thurman C. Thorpe , William G. Barron
IPC分类号： C12Q104
CPC分类号： C12M41/46 , C12M41/36 , C12Q1/04 , C12Q1/18 , G01N21/253 , G01N2035/0425
摘要： A method and apparatus for performing microbial antibiotic susceptibility testing include disposable, multi-chambered susceptibility plates and an automated plate handler and image acquisition and processing instrument is described. The susceptibility plates are inoculated with a suitable microorganism such as bacteria, fungi, protozoa, algae or viruses and anti-microbial agent(s) are applied that the microorganism is exposed to at a variety of concentrations or a gradient of each anti-microbial agent. The plates are then placed in the instrument, which monitors and measures the growth or lack thereof of the microorganisms. This data is used to determine the susceptibility of the microorganism to the antibiotics. Such a system automates anti-microbial susceptibility testing using solid media and Carbo-Bauer standardized result reporting. The system provides a level of automation previously associated only with broth micro dilution testing, while retaining the advantages of the manual disk diffusion test.
摘要翻译：用于进行微生物抗生素敏感性试验的方法和装置包括一次性多室敏感性板和自动板处理器以及图像采集和处理仪器。敏感性板用合适的微生物如细菌，真菌，原生动物，藻类或病毒接种，并且应用抗微生物剂，微生物以各种浓度或每种抗微生物剂的梯度暴露。然后将板放置在仪器中，其监测和测量微生物的生长或缺乏。该数据用于确定微生物对抗生素的敏感性。这种系统使用固体培养基和Carbo-Bauer标准化结果报告自动化抗微生物敏感性测试。该系统提供以前仅与肉汤微量稀释测试相关的自动化水平，同时保留手动盘扩散测试的优点。

26. 发明授权

US10167494B2 Method for detection, characterization and/or identification of microorganisms in a sealed container 有权
公开(公告)号：US10167494B2
公开(公告)日：2019-01-01
申请号：US12589968
申请日：2009-10-30
申请人： John Walsh , Jones M. Hyman , Thurman Thorpe , Bradford Clay
发明人： John Walsh , Jones M. Hyman , Thurman Thorpe , Bradford Clay
IPC分类号： C12Q1/00 , C12Q1/04 , G01N21/65 , G01N33/68 , G01N21/35 , G01N21/3581 , G01N21/47 , G01N21/64 , G01N21/3577
摘要： The present invention provides a method and system for monitoring, detecting, and/or characterizing a biological particle that may be present in a sample. The method may be accomplished in a sealed container by utilizing a first step time-dependent spectroscopic technique to obtain at least two measurements of a growth composition comprising a sample and correlating said measurements for the detection and/or characterization of a biological particle that may be present in the sample. The method further provides for a subsequent step for the separation, characterizion and/or identification of the microorganisms in the sealed container.

27. 发明授权

US10059975B2 Methods for the isolation and identification of microorganisms 有权
公开(公告)号：US10059975B2
公开(公告)日：2018-08-28
申请号：US12589929
申请日：2009-10-30
申请人： John Walsh , Jones M. Hyman , Thurman Thorpe , Bradford Clay
发明人： John Walsh , Jones M. Hyman , Thurman Thorpe , Bradford Clay
IPC分类号： C12Q1/04 , G01N21/65 , G01N33/68 , G01N21/35 , G01N21/3581 , G01N21/47 , G01N21/64 , G01N21/359
CPC分类号： C12Q1/04 , G01N21/35 , G01N21/3581 , G01N21/359 , G01N21/47 , G01N21/64 , G01N21/65 , G01N33/6848
摘要： The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides methods for separating, characterizing and/or identifying microorganisms in situ within a single system.

28. 发明申请

US20100129858A1 Method for separation, characterization and/or identification of microorganisms using spectroscopy 有权
标题翻译：使用光谱法分离，表征和/或鉴定微生物的方法
公开(公告)号：US20100129858A1
公开(公告)日：2010-05-27
申请号：US12589952
申请日：2009-10-30
申请人： John Walsh , Jones M. Hyman , Thurman Thorpe , Bradford Clay , Christopher Ronsick
发明人： John Walsh , Jones M. Hyman , Thurman Thorpe , Bradford Clay , Christopher Ronsick
IPC分类号： C12Q1/04
CPC分类号： C12Q1/04 , G01N21/35 , G01N21/3581 , G01N21/359 , G01N21/47 , G01N21/64 , G01N21/65 , G01N33/6848 , Y02A50/55
摘要： The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides for spectroscopic interrogation of the separated microorganism sample to produce measurements of the microorganism and characterizing and/or identifying the microorganism in the sample using said spectroscopic measurements.
摘要翻译：本发明涉及一种用于分离，表征和/或鉴定测试样品中的微生物的方法。本发明的方法包括用于裂解可能存在于测试样品中的非微生物细胞的任选裂解步骤，随后是随后的分离步骤。该方法可用于从诸如含血培养基的复杂样品中分离，表征和/或鉴定微生物。本发明还提供分离的微生物样品的光谱询问以产生微生物的测量，并使用所述光谱测量来表征和/或鉴定样品中的微生物。

29. 发明申请

US20100129857A1 Methods for the isolation and identification of microorganisms 审中-公开
标题翻译：微生物分离鉴定方法
公开(公告)号：US20100129857A1
公开(公告)日：2010-05-27
申请号：US12589929
申请日：2009-10-30
申请人： John Walsh , Jones M. Hyman , Thurman Thorpe , Bradford Clay
发明人： John Walsh , Jones M. Hyman , Thurman Thorpe , Bradford Clay
IPC分类号： C12Q1/04 , C12N1/00
CPC分类号： C12Q1/04 , G01N21/35 , G01N21/3581 , G01N21/359 , G01N21/47 , G01N21/64 , G01N21/65 , G01N33/6848
摘要： The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides methods for separating, characterizing and/or identifying microorganisms in situ within a single system.
摘要翻译：本发明涉及一种用于分离，表征和/或鉴定测试样品中的微生物的方法。本发明的方法包括用于裂解可能存在于测试样品中的非微生物细胞的任选裂解步骤，随后是随后的分离步骤。该方法可用于从诸如含血培养基的复杂样品中分离，表征和/或鉴定微生物。本发明还提供了在单个系统内原位分离，表征和/或鉴定微生物的方法。

30. 发明授权

US06596532B1 Device for isolation and surface culture of microorganisms from bulk fluids 有权
标题翻译：用于从散装流体中分离和表面培养微生物的装置
公开(公告)号：US06596532B1
公开(公告)日：2003-07-22
申请号：US09502324
申请日：2000-02-11
申请人： Jones M. Hyman , Paul M. Matsumura , Scott R. Jeffrey , Martin J. Maresch , Thurman C. Thorpe
发明人： Jones M. Hyman , Paul M. Matsumura , Scott R. Jeffrey , Martin J. Maresch , Thurman C. Thorpe
IPC分类号： C12M134
CPC分类号： C12M25/14 , C12Q1/04 , C12Q1/045 , C12Q1/24
摘要： A device and method are provided for isolating and culturing microorganisms from a bulk fluid sample. The device comprises a container having therein a polymeric immobilization layer having interstitial spaces between polymer chains such as a gel matrix. The interstitial spaces are of an average size less than an average size of microorganisms to be separated from the sample and cultured. A bulk fluid sample is applied to the immobilization layer where fluid is absorbed by the layer and microorganisms remain on the surface of the layer. After culturing, microorganism colonies are readily accessible on the surface of the layer for harvest and testing. The immobilization layer may contain one or more of nutrients for microorganism growth, lytic agents, lytic enzymes, antibiotics, antibiotic neutralizers, indicators, detergents and selective agents. An adjacent support layer may be above and/or below the immobilization layer. The immobilization layer may be in combination with a sensor layer that changes color in areas corresponding to portions of the layer having microorganisms thereon. A membrane may be embedded in the immobilization layer for enhancing microorganism visibility and facilitating microorganism harvest.
摘要翻译：提供了用于从大量流体样品中分离和培养微生物的装置和方法。该装置包括其中具有聚合物固定化层的容器，其在聚合物链之间具有间隙，例如凝胶基质。间隙空间的平均尺寸小于从样品中分离的微生物的平均尺寸并进行培养。将大量流体样品施加到固定层，其中流体被层吸收，微生物保留在层的表面上。培养后，微生物菌落易于在层的表面进行收获和测试。固定层可以含有一种或多种用于微生物生长的营养物，溶解剂，溶解酶，抗生素，抗生素中和剂，指示剂，洗涤剂和选择剂。相邻的支撑层可以在固定层的上方和/或下方。固定层可以与在其上具有微生物层的部分相对应的区域中改变颜色的传感器层组合。膜可以嵌入在固定层中以增强微生物的可见性并促进微生物的收获。

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