专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

21. 发明授权

US06855550B2 Expression of G protein coupled receptors in yeast 失效
标题翻译：酵母中G蛋白偶联受体的表达
公开(公告)号：US06855550B2
公开(公告)日：2005-02-15
申请号：US09752145
申请日：2000-12-29
申请人： Klim King , Henrik G. Dohlman , Marc G. Caron , Robert J. Lefkowitz
发明人： Klim King , Henrik G. Dohlman , Marc G. Caron , Robert J. Lefkowitz
IPC分类号： C07K14/39 , C07K14/47 , C07K14/705 , C12N1/18 , C12N1/19 , C12N15/09 , C12N15/12 , C12N15/74 , C12N15/81 , C12P21/02 , C12Q1/00 , C12Q1/02 , C12Q1/68 , C12R1/865 , G01N33/566 , C12P21/04 , G01N33/569
CPC分类号： G01N33/566 , C07K14/39 , C07K14/4722 , C07K14/70571 , C07K2319/00 , C07K2319/02 , C07K2319/32 , C07K2319/61 , C07K2319/705 , C12N15/81 , C12Q1/6897 , G01N2333/726 , G01N2500/00
摘要： Disclosed is a transformed yeast cell containing a first heterologous DNA sequence which codes for a mammalian G protean coupled receptor and a second heterologous DNA sequence which codes for a mammalian G protein α subunit (mammalian Gα). The first and second heterologous DNA sequences are capable of expression in the cell, but the cell is incapable of expressing an endogenous G protein α-subunit (yeast Gα). The cells are useful for screening compounds which affect the rate of dissociation of Gα from Gβτ in a cell.Also disclosed is a novel DNA expression vector useful for making cells as described above. The vector contains a first segment comprising at least a fragment of the extreme amino-terminal coding sequence of a yeast G protein coupled receptor. A second segment is positioned downstream from the first segment (and in correct reading frame therewith), with the second segment comprising a DNA sequence encoding a heterologous G protein coupled receptor.
摘要翻译：公开了含有编码哺乳动物G蛋白偶联受体的第一异源DNA序列和编码哺乳动物G蛋白α亚基（哺乳动物Galpha）的第二异源DNA序列的转化酵母细胞。第一和第二异源DNA序列能够在细胞中表达，但是细胞不能表达内源性G蛋白α亚基（酵母Galpha）。细胞可用于筛选影响细胞中Gbetatau中Galpha解离速率的化合物。还公开了一种用于如上所述制备细胞的新型DNA表达载体。载体含有至少包含酵母G蛋白偶联受体的极端氨基末端编码序列的片段的第一片段。第二片段位于第一片段的下游（并且在其正确的阅读框架中），其中第二片段包含编码异源G蛋白偶联受体的DNA序列。

22. 发明授权

US06528271B1 Inhibition of &bgr;arrestin mediated effects prolongs and potentiates opioid receptor-mediated analgesia 失效
标题翻译：抑制βarrestin介导的作用延长并增强阿片受体介导的镇痛作用
公开(公告)号：US06528271B1
公开(公告)日：2003-03-04
申请号：US09469554
申请日：1999-12-22
申请人： Laura M. Bohn , Fang-Tsyr Lin , Marc G. Caron , Robert J. Lefkowitz
发明人： Laura M. Bohn , Fang-Tsyr Lin , Marc G. Caron , Robert J. Lefkowitz
IPC分类号： C07K1400
CPC分类号： C12N15/8509 , A01K67/0276 , A01K2217/075 , A01K2227/105 , A01K2267/03 , A01K2267/0356 , A01K2267/0393 , C07K14/47 , C07K14/4702 , C07K2319/00 , C07K2319/60 , C12N15/62
摘要： The present invention provides a &bgr;arrestin knockout mouse useful for screeening compounds for efficacy in controlling pain, methods of controlling pain in subjects by inhibiting binding of &bgr;arrestin to phosphorylated &mgr; opioid receptors, and methods of screening a compound for activity in potentiating &mgr; opioid receptor agonist activity (e.g., morphine activity) by determining whether or not said compound inhibits &bgr;arrestin binding to a phosphorylated &mgr; opioid receptor.
摘要翻译：本发明提供了一种可用于抑制化合物治疗疼痛疗效的贝伐星蛋白敲除小鼠，通过抑制βarrestin与磷酸化的阿片样物质受体的结合来控制受试者的疼痛的方法，以及筛选化合物以增强μ阿片受体激动剂活性的活性的方法（例如，吗啡活性），通过确定所述化合物是否抑制βarrestin与磷酸化的阿片样物质受体结合。

23. 发明授权

US06218595B1 Dopamine transporter knockout mice 有权
标题翻译：多巴胺转运蛋白敲除小鼠
公开(公告)号：US06218595B1
公开(公告)日：2001-04-17
申请号：US09134185
申请日：1998-08-14
申请人： Bruno Giros , Mohamed Jaber , Marc G. Caron
发明人： Bruno Giros , Mohamed Jaber , Marc G. Caron
IPC分类号： G01N3315
CPC分类号： C12N15/8509 , A01K67/0276 , A01K2217/075 , A01K2227/105 , A01K2267/03 , A01K2267/0356 , A61K49/0008 , C07K14/47 , C07K14/4702
摘要： A recombinant rodent comprises cells containing a pair genomic dopamine transporter protein alleles, wherein at least one of said alleles is incapable of expressing endogenous dopamine transporter protein. The rodent may be a homozygote, where both of said alleles are incapable of expressing endogenous dopamine transporter protein, or the rodent may be a heterozygote, and one of said alleles expresses endogenous dopamine transporter protein. The rodent is preferably a mouse.
摘要翻译：重组啮齿动物包含含有一对基因组多巴胺转运蛋白等位基因的细胞，其中至少一个所述等位基因不能表达内源性多巴胺转运蛋白。啮齿动物可以是纯合子，其中两个所述等位基因都不能表达内源性多巴胺转运蛋白，或者啮齿动物可以是杂合子，并且所述等位基因之一表达内源性多巴胺转运蛋白。啮齿动物优选是小鼠。

24. 发明授权

US5215915A Cloned gene encoding rat D.sub.1B dopamine receptor 失效
标题翻译：克隆基因编码大鼠D1B多巴胺受体
公开(公告)号：US5215915A
公开(公告)日：1993-06-01
申请号：US686591
申请日：1991-04-16
申请人： Mario Tiberi , Keith R. Jarvie , Marc G. Caron
发明人： Mario Tiberi , Keith R. Jarvie , Marc G. Caron
IPC分类号： C07K14/705 , C12N15/12
CPC分类号： C07K14/70571
摘要： Disclosed is isolated DNA encoding a D.sub.1B -dopamine receptor selected from the group consisting of: (a) isolated DNA which encodes rat D.sub.1B -dopamine receptor; (b) isolated DNA which hybridizes to isolated DNA of (a) above and which encodes a D.sub.1B -dopamine receptor; and (c) isolated DNA differing from the isolated DNAs of (a) and (b) above in codon sequence due to the degeneracy of the genetic code, and which encodes a D.sub.1B dopamine receptor Vectors and host cells containing the same, assay procedures employing D.sub.1B -dopamine receptors, oligonucleotide probes for identifying D.sub.1B -dopamine receptors, and isolated and purified D.sub.1B -dopamine receptors are also disclosed.
摘要翻译：公开了编码D1B-多巴胺受体的分离的DNA，其选自：（a）编码大鼠D1B-多巴胺受体的分离的DNA; （b）分离的DNA，其与上述（a）的分离的DNA杂交并编码D1B-多巴胺受体; 和（c）由于遗传密码的简并性，编码D1B多巴胺受体载体和含有相同的宿主细胞的密码子序列中与上述（a）和（b）的分离的DNA不同的分离的DNA，采用还公开了D1B-多巴胺受体，用于鉴定D1B-多巴胺受体的寡核苷酸探针，以及分离和纯化的D1B-多巴胺受体。

25. 发明授权

US07572888B2 Methods of assaying receptor activity and constructs useful in such methods 有权
公开(公告)号：US07572888B2
公开(公告)日：2009-08-11
申请号：US10785841
申请日：2004-02-24
申请人： Lawrence S. Barak , Marc G. Caron , Stephen S. Ferguson , Jie Zhang
发明人： Lawrence S. Barak , Marc G. Caron , Stephen S. Ferguson , Jie Zhang
IPC分类号： C07K14/00 , C07K17/00 , G01N33/53 , G01N33/567
CPC分类号： C12N15/8509 , A01K67/0276 , A01K2217/075 , A01K2227/105 , A01K2267/03 , A01K2267/0356 , A01K2267/0393 , C07K14/47 , C07K14/4702 , C07K2319/00 , C07K2319/60 , C12N15/62
摘要： Described are methods of detecting G-protein coupled receptor (GPCR) activity in vivo and in vitro; methods of assaying GPCR activity; and methods of screening for GPCR ligands, G protein-coupled receptor kinase (GRK) activity, and compounds that interact with components of the GPCR regulatory process. Constructs useful in such methods are described.

26. 发明授权

US07018812B2 Modified G-protein coupled receptors 有权
公开(公告)号：US07018812B2
公开(公告)日：2006-03-28
申请号：US09993844
申请日：2001-11-05
申请人： Robert H. Oakley , Lawrence S. Barak , Stephane A. Laporte , Marc G. Caron
发明人： Robert H. Oakley , Lawrence S. Barak , Stephane A. Laporte , Marc G. Caron
IPC分类号： C07K14/705 , C07K19/00 , C12N15/62
CPC分类号： C07K14/705
摘要： The present invention relates to modified G-protein coupled receptors (GPCRs). The modified GPCRs of the present invention include GPCRs that have been modified to have carboxyl terminal tails comprising one or more sites of phosphorylation, preferably one or more clusters of phosphorylation sites. The modified GPCRs of the present invention may comprise a retained portion of a carboxyl-terminus region from a first GPCR fused to a polypeptide, wherein the polypeptide comprises the one or more clusters of phosphorylation. The present invention also relates to methods of screening compounds and sample solutions for GPCR activity using the modified GPCRs.

27. 发明授权

US06770449B2 Methods of assaying receptor activity and constructs useful in such methods 有权
公开(公告)号：US06770449B2
公开(公告)日：2004-08-03
申请号：US09772644
申请日：2001-01-30
申请人： Lawrence S. Barak , Marc G. Caron , Stephen S. Ferguson , Jie Zhang
发明人： Lawrence S. Barak , Marc G. Caron , Stephen S. Ferguson , Jie Zhang
IPC分类号： G01N33567
CPC分类号： C12N15/8509 , A01K67/0276 , A01K2217/075 , A01K2227/105 , A01K2267/03 , A01K2267/0356 , A01K2267/0393 , C07K14/47 , C07K14/4702 , C07K2319/00 , C07K2319/60 , C12N15/62
摘要： Described are methods of detecting G-protein coupled receptor (GPCR) activity in vivo and in vitro; methods of assaying GPCR activity; and methods of screening for GPCR ligands, G protein-coupled receptor kinase (GRK) activity, and compounds that interact with components of the GPCR regulatory process. Constructs useful in such methods are described.

28. 发明授权

US06168927A Expression of G protein coupled receptors in yeast 失效
标题翻译：酵母中G蛋白偶联受体的表达
公开(公告)号：US06168927A
公开(公告)日：2001-01-02
申请号：US09056920
申请日：1998-04-08
申请人： Klim King , Henrik G. Dohlman , Marc G. Caron , Robert J. Lefkowitz
发明人： Klim King , Henrik G. Dohlman , Marc G. Caron , Robert J. Lefkowitz
IPC分类号： G01N3353
CPC分类号： G01N33/566 , C07K14/39 , C07K14/4722 , C07K14/70571 , C07K2319/00 , C07K2319/02 , C07K2319/32 , C07K2319/61 , C07K2319/705 , C12N15/81 , C12Q1/6897 , G01N2333/726 , G01N2500/00
摘要： Disclosed is a transformed yeast cell containing a first heterologous DNA sequence which codes for a mammalian G protein coupled receptor and a second heterologous DNA sequence which codes for a mammalian G protein &agr; subunit (mammalian G&agr;). The first and second heterologous DNA sequences are capable of expression in the cell, but the cell is incapable of expressing an endogenous G protein &agr;-subunit (yeast G&agr;) The cells are useful for screening compounds which affect the rate of dissociation of G&agr; from G&bgr;&ggr; in a cell. Also disclosed is a novel DNA expression vector useful for making cells as described above. The vector contains a first segment comprising at least a fragment of the extreme amino-terminal coding sequence of a yeast G protein coupled receptor. A second segment is positioned downstream from the first segment (and in correct reading frame therewith), with the second segment comprising a DNA sequence encoding a heterologous G protein coupled receptor.
摘要翻译：公开了含有编码哺乳动物G蛋白偶联受体的第一异源DNA序列和编码哺乳动物G蛋白α亚基（哺乳动物Galpha）的第二异源DNA序列的转化酵母细胞。第一和第二异源DNA序列能够在细胞中表达，但是细胞不能表达内源性G蛋白α亚基（酵母Galpha）。细胞可用于筛选影响Galeta从Gbetagamma分离速率的化合物在一个单元格还公开了用于如上所述制备细胞的新型DNA表达载体。载体含有至少包含酵母G蛋白偶联受体的极端氨基末端编码序列的片段的第一片段。第二片段位于第一片段的下游（并且在其正确的阅读框架中），其中第二片段包含编码异源G蛋白偶联受体的DNA序列。

29. 发明授权

US6096705A Inhibition of agonist-specific desensitization of .beta.2 adrenergic receptors 失效
标题翻译：抑制β2肾上腺素能受体激动剂特异性脱敏作用
公开(公告)号：US6096705A
公开(公告)日：2000-08-01
申请号：US38072
申请日：1993-03-29
申请人： Robert J. Lefkowitz , Martin J. Lohse , Jeffrey L. Benovic , Marc G. Caron
发明人： Robert J. Lefkowitz , Martin J. Lohse , Jeffrey L. Benovic , Marc G. Caron
IPC分类号： A61K31/21 , A61K31/66 , A61K31/675 , A61K38/16 , A61K38/17 , G01N33/566 , G01N33/573 , A61K31/715 , C12N9/12
CPC分类号： A61K31/715 , A61K31/21 , A61K31/66 , A61K31/675 , A61K38/1787 , G01N33/566 , G01N33/573
摘要： The present invention relates to a method of inhibiting desensitization of a cell to the effects of a compound. The method comprises contacting the cell with an agent capable of inhibiting phosphorylation, by a protein kinase, of a receptor for the compound present on the surface of the cell. The present invention also relates to a method of screening a compound for its ability to inhibit desensitization. The method comprises: i) contacting a receptor specific kinase-containing sample with the compound under conditions such that interaction between receptor specific kinase present in the sample and the compound can occur, andii) determining the ability of the receptor specific kinase to phosphorylate the receptor for which it is specific.
摘要翻译：本发明涉及抑制细胞对化合物作用的脱敏的方法。该方法包括使细胞与能够通过蛋白激酶抑制存在于细胞表面的化合物的受体的磷酸化的试剂接触。本发明还涉及一种筛选化合物抑制脱敏能力的方法。该方法包括：i）使受体特异性激酶样品与化合物在使得存在于样品中的受体特异性激酶与化合物之间发生相互作用的条件下接触，以及ii）确定受体特异性激酶磷酸化的能力受体是特异性的。

30. 发明授权

US5866756A Dopamine transporter knockout mice 失效
标题翻译：多巴胺转运蛋白敲除小鼠
公开(公告)号：US5866756A
公开(公告)日：1999-02-02
申请号：US724405
申请日：1996-10-02
申请人： Bruno Giros , Mohamed Jaber , Marc G. Caron
发明人： Bruno Giros , Mohamed Jaber , Marc G. Caron
IPC分类号： A61K49/00 , C07K14/47 , C12N15/85 , C12N5/00 , C12N15/00
CPC分类号： C12N15/8509 , A01K67/0276 , A61K49/0008 , C07K14/47 , C07K14/4702 , A01K2217/075 , A01K2227/105 , A01K2267/03 , A01K2267/0356
摘要： A recombinant rodent comprises cells containing a pair of genomic dopamine transporter protein alleles, wherein at least one of said alleles is incapable of expressing endogenous dopamine transporter protein. The rodent may be a homozygote, where both of said alleles are incapable of expressing endogenous dopamine transporter protein, or the rodent may be a heterozygote, and one of said alleles expresses endogenous dopamine transporter protein. The rodent is preferably a mouse.
摘要翻译：重组啮齿动物包含含有一对基因组多巴胺转运蛋白等位基因的细胞，其中所述等位基因中的至少一个不能表达内源性多巴胺转运蛋白。啮齿动物可以是纯合子，其中两个所述等位基因都不能表达内源性多巴胺转运蛋白，或者啮齿动物可以是杂合子，并且所述等位基因之一表达内源性多巴胺转运蛋白。啮齿动物优选是小鼠。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式