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21. 发明申请

WO2014043143A1 NUCLEIC ACID AMPLIFICATION 审中-公开
标题翻译：核酸放大
公开(公告)号：WO2014043143A1
公开(公告)日：2014-03-20
申请号：PCT/US2013/059093
申请日：2013-09-10
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： LI, Chieh-Yuan , RUFF, David , CHEN, Shiaw-Min , O'NEIL, Jennifer , KASINSKAS, Rachel , ROTHBERG, Jonathan , HINZ, Wolfgang
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q2521/507 , C12Q2527/119 , C12Q2535/122 , C12Q2565/537 , C12Q2565/607
摘要： In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.
摘要翻译：在一些实施方案中，本发明提供核酸扩增方法，包括形成反应混合物，并使反应混合物经受适合于核酸扩增的条件。在一些实施方案中，用于核酸扩增的方法包括将待扩增的核酸进行部分变性的条件。在一些实施方案中，用于核酸扩增的方法包括扩增而不完全变性扩增的核酸。在一些实施方案中，用于核酸扩增的方法使用催化同源重组的酶和聚合酶。在一些实施方案中，用于核酸扩增的方法可以在单个反应容器中进行。在一些实施方案中，用于核酸扩增的方法可以在反应混合物的单个连续液相中进行，而不需要分隔反应混合物或固定反应组分。在一些实施方案中，用于核酸扩增的方法包括在等温扩增条件下任选地在聚合物存在下在表面上扩增至少一种多核苷酸。聚合物可以包括筛分剂和/或扩散还原剂。

22. 发明申请

WO2013119936A2 HYDROPHILIC POLYMERIC PARTICLES AND METHODS FOR MAKING SAME 审中-公开
标题翻译：水解聚合物颗粒及其制备方法
公开(公告)号：WO2013119936A2
公开(公告)日：2013-08-15
申请号：PCT/US2013/025328
申请日：2013-02-08
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： FONNUM, Geir , MODAHL, Grete , KJUS, Nini , MOLTEBERG, Astrid Evenroed , TRAN, Diem , AASERUD, Jo , GOKMEN, M. Talha , MENCHEN, Steven , FULLER, Carl , ANDRUZZI, Luisa , HINZ, Wolfgang , GREMYACHINSKIY, Dmitriy , LIGHT, David , MASTROIANNI, Alexander , ROSENBLUM, Barnett , KHAN, Shaheer , STOLARCZYK, Craig , SOKOLSKY, Tanya
IPC分类号： C08F2/16 , C08F230/08
CPC分类号： C08F130/08 , C07F7/1852 , C07F7/188 , C08F2/20 , C08F2/26 , C08F6/24 , C08F8/12 , C08F8/32 , C08F8/34 , C08F220/58 , C08F222/385 , C08F230/08 , C08F2800/20 , C08J9/20 , C08J2343/04 , C12P19/34 , C12Q1/686 , C12Q1/6874 , G01N27/4145 , Y10T428/2982 , C08F257/02 , C08F2222/1013 , C08F212/36 , C08F220/60 , C12Q2563/149 , C12Q2563/159 , C12Q2565/543
摘要： A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.
摘要翻译：形成颗粒的方法包括在水性悬浮液中的分散相中聚合具有疏水保护基团的亲水性单体的多个聚合单元，从而形成包含多个疏水保护基团的聚合物颗粒。该方法还包括将聚合物颗粒转化为亲水性颗粒。

23. 发明申请

WO2013109886A1 ISFET SENSOR ARRAY COMPRISING TITANIUM NITRIDE AS A SENSING LAYER LOCATED THE BOTTOM OF A MICROWELL STRUCTURE 审中-公开
标题翻译： ISFET传感器阵列包含硝酸铁作为传感层位于MICROWELL结构的底部
公开(公告)号：WO2013109886A1
公开(公告)日：2013-07-25
申请号：PCT/US2013/022140
申请日：2013-01-18
申请人： LIFE TECHNOLOGIES CORPORATION , BUSTILLO, James , REARICK, Todd , HINZ, Wolfgang , FIFE, Keith
发明人： BUSTILLO, James , REARICK, Todd , HINZ, Wolfgang , FIFE, Keith
IPC分类号： G01N27/414
CPC分类号： B01L3/5027 , B01L3/502707 , B01L2200/0668 , B01L2300/0636 , B01L2300/0645 , B01L2300/0819 , B01L2300/0877 , B01L2300/0893 , G01N27/4145
摘要： A method of fabricating a microwell in an array structure is disclosed herein. The array structure includes a plurality of field effect transistors (FETs), where each FET has a gate structure. The method includes disposing a titanium nitride (TiN) layer on at least one conductive layer coupled to the gate structure of at least one FET. A insulation layer is disposed on the array structure, where the insulation layer lies above the TiN layer. Further, an opening above the gate structure of the at least one FET is etched to remove the insulation layer above the gate structure and to expose the TiN layer. A microwell with at least one sidewall formed from the insulation layer and with a bottom surface formed from the TiN layer is a result of the etching process. The gate structure is specified as a floating gate structure and the FET is an ISFET.
摘要翻译：本文公开了一种制造阵列结构中的微孔的方法。阵列结构包括多个场效应晶体管（FET），其中每个FET具有栅极结构。该方法包括在耦合到至少一个FET的栅极结构的至少一个导电层上设置氮化钛（TiN）层。绝缘层设置在阵列结构上，其中绝缘层位于TiN层上方。此外，蚀刻至少一个FET的栅极结构之上的开口以去除栅极结构上方的绝缘层并暴露TiN层。具有由绝缘层形成的至少一个侧壁和由TiN层形成的底表面的微孔是蚀刻工艺的结果。栅极结构被指定为浮置栅极结构，FET是ISFET。

24. 发明公开

EP4095264A1 SURFACE STABILIZATION OF BIOSENSORS 审中-实审
公开(公告)号：EP4095264A1
公开(公告)日：2022-11-30
申请号：EP22185816.0
申请日：2016-03-04
申请人： Life Technologies Corporation
发明人： WAGGONER, Phil , BALL, James , HINZ, Wolfgang , MINTO, Michael , PARKER, Scott , COX, David , MASTROIANNI, Alexander , GRAY, Jeremy , GLAZER, Marc , GORRELL, Kimberly
IPC分类号： C12Q1/6869 , G01N27/414
摘要： A sensor apparatus includes a substrate, a semiconductor device disposed over the substrate, the semiconductor device having a surface electrode structure, and a saccharide coating formed over the surface electrode structure. The saccharide coating can be removed prior to use. The semiconductor device can further include a well and optionally a bead disposed in the well.

25. 发明公开

EP3265585A1 SURFACE STABILIZATION OF BIOSENSORS 有权
标题翻译：生物传感器的表面稳定化
公开(公告)号：EP3265585A1
公开(公告)日：2018-01-10
申请号：EP16711079.0
申请日：2016-03-04
申请人： Life Technologies Corporation
发明人： WAGGONER, Phil , BALL, James , HINZ, Wolfgang , MINTO, Michael , PARKER, Scott , COX, David , MASTROIANNI, Alexander , GRAY, Jeremy , GLAZER, Marc , GORRELL, Kimberly
IPC分类号： C12Q1/68 , G01N27/414
CPC分类号： C12Q1/6869 , G01N27/4145 , C12Q2527/119 , C12Q2527/125 , C12Q2565/607
摘要： A sensor apparatus includes a substrate, a semiconductor device disposed over the substrate, the semiconductor device having a surface electrode structure, and a saccharide coating formed over the surface electrode structure. The saccharide coating can be removed prior to use. The semiconductor device can further include a well and optionally a bead disposed in the well.

26. 发明公开

EP2762872A1 Methods and apparatus for measuring analytes using large scale FET arrays 有权
标题翻译：大规模方法和装置的分析物测量通过FET阵列的方法
公开(公告)号：EP2762872A1
公开(公告)日：2014-08-06
申请号：EP14152861.2
申请日：2007-12-14
申请人： Life Technologies Corporation
发明人： ROTHBERG, Jonathan M. , HINZ, Wolfgang , JOHNSON, Kim L. , BUSTILLO, James
IPC分类号： G01N27/414 , G01N33/543
CPC分类号： G01N27/4148 , C12Q1/6818 , C12Q1/6874 , G01N27/4145 , G01N33/54373 , G01N33/5438 , H01L27/088 , H01L29/42324 , H01L29/78 , H01L2924/01006 , H01L2924/01013 , H01L2924/01015 , H01L2924/01073 , H01L2924/14 , H01L2924/1433 , Y10T436/22 , C12Q2565/607 , C12Q2565/301 , C12Q2533/101
摘要： A circuit may include an array of CMOS-fabricated pixels formed in columns and rows. Each pixel (105) includes: a chemically-sensitive field effect transistor (chemFET) (150), a first switch (Q3), and a second switch (Q2). The chemFET has a floating gate structure, a source terminal, and a drain terminal that is coupled to a first readout signal line (116). The first switch couples the source terminal of the chemFET to a current source (106) and has one first transistor. The second switch couples the source terminal of the chemFET to a second readout signal line (114) and has one second transistor. The first and second switches are controlled by a row selection line (118) coupled to the two switches.
摘要翻译：一种电路可以包括在制造形成在列和行的CMOS像素阵列。各像素（105）包括：化学敏感的场效应晶体管（个chemFET）（150），第一开关（Q3），和第二开关（Q2）。所述chemFET具有浮动栅极结构，源极端子和漏极端子也被连接到第一读出信号线（116）。第一开关耦合在chemFET的源极端子到电流源（106），并具有一个第一晶体管。所述第二开关耦合在chemFET的源极端子到第二读出信号线（114）和具有一个第二晶体管。第一和第二开关由耦合到两个开关的行选择线（118）控制。

27. 发明公开

EP2321434A2 METHODS FOR SEQUENCING INDIVIDUAL NUCLEIC ACIDS UNDER TENSION 审中-公开
标题翻译：方法的核酸测序单ENERGIZED
公开(公告)号：EP2321434A2
公开(公告)日：2011-05-18
申请号：EP09805290.5
申请日：2009-08-07
申请人： Life Technologies Corporation
发明人： ROTHBERG, Jonathan, M. , LEAMON, John, H. , DAVIDSON, John, F. , VAN OIJEN, Antoine, M. , HINZ, Wolfgang , DAVEY, Melville , HANN, Bradley , SCHULTZ, Jonathan
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q2523/303 , C12Q2565/131 , C12Q2525/204 , C12Q2521/101 , C12Q2535/122
摘要： The invention provides apparatuses and methods of use thereof for sequencing nucleic acids subjected to a force, and thus considered under tension. The methods may employ but are not dependent upon incorporation of extrinsically detectably labeled nucleotides.

28. 发明公开

EP4134667A1 APPARATUS FOR MEASURING ANALYTES USING FET ARRAYS 审中-实审
公开(公告)号：EP4134667A1
公开(公告)日：2023-02-15
申请号：EP22179321.9
申请日：2007-12-14
申请人： Life Technologies Corporation
发明人： ROTHBERG, Johnathan M , HINZ, Wolfgang , JOHNSON, Kim L , BUSTILLO, James
IPC分类号： G01N27/414 , G01N33/543 , C12Q1/6874 , C12Q1/68 , H01L29/78 , H01L29/423 , H01L27/088
摘要： Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g. ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.

29. 发明公开

EP3489367A1 SURFACE STABILIZATION OF BIOSENSORS 有权
公开(公告)号：EP3489367A1
公开(公告)日：2019-05-29
申请号：EP18213839.6
申请日：2016-03-04
申请人： Life Technologies Corporation
发明人： WAGGONER, Phil , BALL, James , HINZ, Wolfgang , MINTO, Michael , PARKER, Scott , COX, David , MASTROIANNI, Alexander , GRAY, Jeremy , GLAZER, Marc , GORRELL, Kimberly
IPC分类号： C12Q1/6869 , G01N27/414
摘要： A sensor apparatus includes a substrate, a semiconductor device disposed over the substrate, the semiconductor device having a surface electrode structure, and a saccharide coating formed over the surface electrode structure. The saccharide coating can be removed prior to use. The semiconductor device can further include a well and optionally a bead disposed in the well.

30. 发明公开

EP3040424A1 MODIFIED PROTEINS AND METHODS OF MAKING AND USING SAME 有权
标题翻译：改良蛋白酶自身VERFAHREN ZU IHRER HERSTELLUNG UND VERWENDUNG
公开(公告)号：EP3040424A1
公开(公告)日：2016-07-06
申请号：EP15192848.8
申请日：2011-02-28
申请人： Life Technologies Corporation
发明人： DAVIDSON, John F. , HINZ, Wolfgang , ROTHBERG, Jonathan , WHITAKER, Richard
IPC分类号： C12Q1/68 , C12Q1/48 , C12N9/12
CPC分类号： C12N9/1252
摘要： Methods, compositions, systems, and kits comprising modified proteins, particularly modified nucleic acid-binding proteins and DNA polymerases with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pKa values are described. Furthermore, the invention provides methods for using such modified proteins in ion-producing reactions, such as ion-based nucleic acid sequencing reactions.
摘要翻译：提供了包含修饰的蛋白质，特别是经修饰的核酸结合蛋白质和具有改变的缓冲性质的DNA聚合酶的方法，组合物，系统和试剂盒。例如，在一些实施方案中，描述了形成包含一个或多个氨基酸修饰以获得所需pKa值的修饰蛋白质的方法。此外，本发明提供了在离子产生反应中使用这种修饰的蛋白质的方法，例如基于离子的核酸测序反应。

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