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21. 发明授权

US4708939A Process and reagent for the complete and rapid removal of a turbidity in a biological fluid 失效
标题翻译：用于完全和快速地去除生物流体中的浊度的方法和试剂
公开(公告)号：US4708939A
公开(公告)日：1987-11-24
申请号：US803496
申请日：1985-12-02
申请人： Joachim Siedel , Johnny Staepels , Joachim Ziegenhorn
发明人： Joachim Siedel , Johnny Staepels , Joachim Ziegenhorn
IPC分类号： G01N33/48 , B01F17/02 , B01F17/34 , G01N33/53 , G01N33/536 , G01N33/92 , G01N31/00
CPC分类号： G01N33/92 , Y10T436/104165 , Y10T436/107497 , Y10T436/25125
摘要： The present invention provides an agent for the removal of a turbidity in a biological fluid, wherein it contains(a) a polyethoxylated triglyceride with an HLB value of 4 to 14,(b) a secondary n-alkane sulphonate, as well as optionally(c) a futher non- or anionic tenside, in aqueous, optionally buffered solution.
摘要翻译：本发明提供了用于去除生物流体中的浊度的试剂，其中其包含（a）HLB值为4至14的聚乙氧基化甘油三酸酯，（b）仲正烷烃磺酸二酯以及任选的（ c）在水性，任选缓冲溶液中的另外的非或者阴离子表面活性剂。

22. 发明授权

US4551427A Process and reagent for the determination of blood glucose in haemolysed whole blood 失效
标题翻译：用于测定溶血全血中血糖的方法和试剂
公开(公告)号：US4551427A
公开(公告)日：1985-11-05
申请号：US575409
申请日：1984-01-30
申请人： Brigitte Draeger , Joachim Ziegenhorn
发明人： Brigitte Draeger , Joachim Ziegenhorn
IPC分类号： G01N33/66 , C12Q1/32 , C12Q1/48 , C12Q1/54
CPC分类号： C12Q1/54
摘要： A reagent and improved process for the determination of D-glucose in body fluids by reaction with ATP in the presence of hexokinase, glucose-6-phosphate dehydrogenase, NADP, Mg.sup.++ ions and buffer and measurement of the NADPH formed. The improved process requires, before reaction with ATP, the mixing of the whole blood with a reagent which is composed of 0.01 to 0.5% weight/volume of an alkyl sulphate or sulphonate or of an alkyl-aryl sulphonate wherein the alkyl moiety is C.sub.8 to C.sub.32 and optionally contains one or more hydroxylalkylamino radicals as substituents and/or is interrupted by one or more ether oxygen atoms and the alkyl-aryl radicals having C.sub.8 to C.sub.18 alkyls and optionally a preserving agent.
摘要翻译：在己糖激酶，葡萄糖-6-磷酸脱氢酶，NADP，Mg ++离子和缓冲液的存在下与ATP反应测定体液中D-葡萄糖的试剂和改进方法，并测量形成的NADPH。改进的方法需要在与ATP反应之前，将全血与由0.01至0.5％重量/体积的烷基硫酸盐或磺酸盐或烷基 - 芳基磺酸盐组成的试剂混合，其中烷基部分为C8至 C32并且任选地含有一个或多个羟烷基氨基作为取代基和/或被一个或多个醚氧原子间隔，并且具有C8-C18烷基的烷基 - 芳基和任选的保存剂。

23. 发明授权

US4503144A Method and composition for the determination of cholesterol 失效
标题翻译：测定胆固醇的方法和组成
公开(公告)号：US4503144A
公开(公告)日：1985-03-05
申请号：US328350
申请日：1981-12-07
申请人： Rolf Deeg , Helmut Schlumberger , Joachim Ziegenhorn
发明人： Rolf Deeg , Helmut Schlumberger , Joachim Ziegenhorn
IPC分类号： G01N33/50 , C12Q1/60 , C12Q1/26 , C12Q1/28 , C12Q1/44 , C12R1/465
CPC分类号： C12Q1/60 , Y10S435/805 , Y10S435/81 , Y10S435/886
摘要： The present invention provides a process for the determination of cholesterol in free or bound form by means of cholesterol oxidase and optionally of cholesterol esterase by measurement of the oxygen consumption, of the hydrogen peroxide formed or of the cholestenone formed, wherein the determination is carried out kinetically with the use of a cholesterol oxidase which has been obtained from a micro-organism of the genus Streptomyces and with the addition of 3,4-dichlorophenol.The present invention also provides a reagent for the kinetic determination of cholesterol, comprising cholesterol oxidase and a system for the determination of hydrogen peroxide or of cholestenone and optionally cholesterol esterase, wherein it additionally contains 3,4-dichlorophenol and the cholesterol oxidase used originates from a micro-organism of the genus Streptomyces.
摘要翻译：本发明提供了一种通过胆固醇氧化酶和任选的胆固醇酯酶通过测量所形成的过氧化氢或形成的胆碱酯的氧消耗来测定游离或结合形式的胆固醇的方法，其中进行测定动态地使用已经从链霉菌属的微生物获得的胆固醇氧化酶，并加入3,4-二氯苯酚。本发明还提供用于胆固醇的动力学测定的试剂，其包含胆固醇氧化酶和用于测定过氧化氢或胆石素和任选的胆固醇酯酶的系统，其中还含有3,4-二氯苯酚，所用的胆固醇氧化酶源自链霉菌属的微生物。

24. 发明授权

US5094943A Process and reagent for the improved quantitative colorimetic determination of hydrogen peroxide 失效
标题翻译：改进过氧化氢定量色谱法的方法和试剂
公开(公告)号：US5094943A
公开(公告)日：1992-03-10
申请号：US147576
申请日：1988-01-19
申请人： Joachim Siedel , Albert Roder , Joachim Ziegenhorn
发明人： Joachim Siedel , Albert Roder , Joachim Ziegenhorn
IPC分类号： C12Q1/26 , C12Q1/28
CPC分类号： C12Q1/28 , C12Q1/26
摘要： The present invention provides a process for the improved colorimetric determination of hydrogen peroxide as formed by a hydrogen peroxide-producing oxidase, by addition of a chromogenic system and measurement of the colored material formed, wherein superoxide dismutase (E.C. 1.15.1.1) is added to the reagent solution.The present invention also provides a reagent for the improved colorimetric determination of hydrogen peroxide, comprising a hydrogen peroxide-producing oxidase, a chromogenic system, a buffer and optionally adjuvant enzymes, wherein it also contains superoxide dismutase.
摘要翻译：本发明提供了通过添加显色系统和形成的有色材料的测量来改进由产生过氧化氢的氧化酶形成的过氧化氢的比色测定方法，其中将超氧化物歧化酶（EC 1.15.1.1）加入到试剂溶液。本发明还提供了用于改进过氧化氢比色测定的试剂，其包含产生过氧化氢的氧化酶，显色系统，缓冲液和任选的辅助酶，其中还含有超氧化物歧化酶。

25. 发明授权

US5013647A Removal of interfering reducing substances with tetrazolium salts in redox measurement reactions 失效
标题翻译：在氧化还原测定反应中用四唑盐除去干扰还原物质
公开(公告)号：US5013647A
公开(公告)日：1991-05-07
申请号：US35617
申请日：1987-04-03
申请人： Michael-Harold Town , Joachim Siedel , Joachim Ziegenhorn
发明人： Michael-Harold Town , Joachim Siedel , Joachim Ziegenhorn
IPC分类号： C07D257/04 , C12Q1/26 , C12Q1/28 , G01N33/50
CPC分类号： C07D257/04 , C12Q1/26 , C12Q1/28 , C12Q2326/92 , Y10S435/805
摘要： A process for the determination of substrate or enzyme activities by the use of a redox reaction as a measurement reaction is carried out in the presence of one or more additionally added tetrazolium salts to remove disturbing substances. The tetrazolium salts have the formula ##STR1## in which R.sup.1 is a hydrogen atom, a carboxyl group or an alkyl, phenyl, nitrophenyl, dinitrophenyl, carboxyl-substituted phenyl or trialkylammoniumphenyl radical, R.sup.2 is a phenyl, nitrophenyl, biphenylyl or naphthyl radical, R.sup.3 is a phenyl, carboxyl-substituted phenyl, carboxyl-substituted hydroxyphenyl or dimethylthiazolyl radical, and A.sup..crclbar. is a monovalent anion. The formazanes formed by reaction with reducing substances do not absorb light at all, or absorb light only to a negligible extent, at the measurement wavelength of the redox reaction. New compounds included within the formula are those in which R.sup.1 is carboxy, phenyl, 2-carboxy, phenyl, 2-carboxyphenyl, 4-carboxyphenyl, 2,4-dinitrophenyl or 4-trimethylammoniumphenyl, R.sup.2 is phenyl, and R.sup.3 is phenyl or 2-carboxyphenyl; with the proviso that, when R.sup.3 is unsubstituted phenyl or 2-carboxyphenyl, R.sup.1 may not be unsubstituted phenyl.
摘要翻译：通过使用氧化还原反应作为测量反应来测定底物或酶活性的方法在一种或多种另外加入的四唑鎓盐的存在下进行以除去扰乱物质。所述四唑鎓盐具有其中R 1为氢原子，羧基或烷基，苯基，硝基苯基，二硝基苯基，羧基取代的苯基或三烷基铵苯基的式（I），R 2为苯基，硝基苯基，联苯基或萘基，R3是苯基，羧基取代的苯基，羧基取代的羟基苯基或二甲基噻唑基，A（ - ）是一价阴离子。通过与还原物质反应而形成的甲烷在氧化还原反应的测定波长下根本不吸收光，或仅吸收光可忽略的程度。包括在式中的新化合物是其中R1是羧基，苯基，2-羧基，苯基，2-羧基苯基，4-羧基苯基，2,4-二硝基苯基或4-三甲基铵苯基，R2是苯基，R3是苯基或2 -carboxyphenyl; 条件是当R3是未取代的苯基或2-羧基苯基时，R 1可以不是未被取代的苯基。

26. 发明授权

US4895799A Process and reagent for the determination of total bilirubin 失效
标题翻译：用于测定总胆红素的方法和试剂
公开(公告)号：US4895799A
公开(公告)日：1990-01-23
申请号：US17662
申请日：1987-02-24
申请人： Cornelia Kruse-Muller , Joachim Siedel , Joachim Ziegenhorn
发明人： Cornelia Kruse-Muller , Joachim Siedel , Joachim Ziegenhorn
IPC分类号： C12Q1/26 , C12Q1/37 , G01N33/72
CPC分类号： C12Q1/26 , C12Q1/37 , Y10S435/81
摘要： The present invention provides a process for the determination of total bilirubin in samples of body fluids. The method comprises the incubation of a sample of body fluid with both subtilisin which completely liberates bilirubin from its albumin and with bilirubin oxidase as a measurement of total bilirubin content in said body fluid sample.
摘要翻译：本发明提供了一种测定体液样品中总胆红素的方法。该方法包括将体液样品与从其白蛋白完全释放胆红素的枯草杆菌蛋白酶和胆红素氧化酶一起孵育，作为所述体液样品中总胆红素含量的量度。

27. 发明授权

US4868139A Aqueous cholesterol standard solution and process for its preparation 失效
标题翻译：水性胆固醇标准溶液及其制备方法
公开(公告)号：US4868139A
公开(公告)日：1989-09-19
申请号：US122019
申请日：1987-11-17
申请人： Rolf Deeg , Gisela Dengler , Joachim Ziegenhorn
发明人： Rolf Deeg , Gisela Dengler , Joachim Ziegenhorn
IPC分类号： G01N33/50 , C12Q1/60 , G01N33/92
CPC分类号： C12Q1/60 , Y10T436/104165 , Y10T436/108331
摘要： The present invention provides an aqueous cholesterol standard solution with a definite content of cholesterol, wherein it contains a detergent mixture of 10 to 90% of cholic acid and 90 to 10% of desoxycholic acid or of appropriate salts or derivatives of these acids.The present invention also provides a process for the preparation of this aqueous cholesterol standard solution, wherein a detergent mixture of cholic acid and desoxycholic acid or of appropriate salts and derivatives of these acids is dissolved in distilled water or in 0.9% aqueous sodium chloride solution, an appropriate preservation agent and/or a buffer effective in the pH range of from 7 to 9 optionally added thereto, and a definite, precisely defined amount of cholesterol is dissolved in the solution thus obtained, while stirring and warming to 40.degree. to 60.degree. C.
摘要翻译：本发明提供具有一定胆固醇含量的胆固醇水溶液，其中含有10-90％胆酸和90-10％脱氧胆酸或这些酸的适当盐或衍生物的洗涤剂混合物。本发明还提供了制备这种胆固醇水溶液标准溶液的方法，其中将胆酸和脱氧胆酸的洗涤剂混合物或这些酸的适当盐和衍生物溶解在蒸馏水中或在0.9％氯化钠水溶液中，适当的保存剂和/或在任意添加的pH范围为7〜9的有效缓冲液中，将一定精确定量的胆固醇溶于所得溶液中，同时搅拌并升温至40〜60℃ C。

28. 发明授权

US4746605A Process and a reagent for the determination of low density lipoproteins (LDL) 失效
标题翻译：用于测定低密度脂蛋白（LDL）的方法和试剂
公开(公告)号：US4746605A
公开(公告)日：1988-05-24
申请号：US656954
申请日：1984-10-02
申请人： Lorenz Kerscher , Joachim Ziegenhorn , Sigbert Schiefer
发明人： Lorenz Kerscher , Joachim Ziegenhorn , Sigbert Schiefer
IPC分类号： G01N33/53 , C12Q1/00 , C12Q1/60 , G01N33/531 , G01N33/536 , G01N33/68 , G01N33/92
CPC分类号： C12Q1/60 , G01N33/92 , G01N2800/044 , Y10S435/962 , Y10S436/817 , Y10S436/825 , Y10S436/826 , Y10T436/25125
摘要： A method for determining low density lipoproteins (LDLs) in a body fluid sample, as well as a reagent suited for this use, are taught. The method involves precipitating high density lipoproteins (HDLs) from the sample, using an HDL specific antibody or reactive fragment, and then determining the presence and amount of LDL in the supernatant which results. The reagent contains the HDL specific antibodies, as well as polyanions and divalent cations.
摘要翻译：教导了体液样品中低密度脂蛋白（LDL）的测定方法，以及适用于此用途的试剂。该方法包括使用HDL特异性抗体或活性片段从样品中沉淀高密度脂蛋白（HDL），然后测定上清液中LDL的存在和量。试剂含有HDL特异性抗体，以及聚阴离子和二价阳离子。

29. 发明授权

US4727025A Process and reagent for the fully enzymatic determination of urea 失效
标题翻译：用于全面酶法测定尿素的方法和试剂
公开(公告)号：US4727025A
公开(公告)日：1988-02-23
申请号：US823489
申请日：1986-01-10
申请人： Joachim Siedel , August W. Wahlefeld , Joachim Ziegenhorn
发明人： Joachim Siedel , August W. Wahlefeld , Joachim Ziegenhorn
IPC分类号： G01N33/62 , C12N9/04 , C12Q1/56 , C12Q1/58
CPC分类号： C12N9/0006 , C12Q1/58 , C12Y101/01154
摘要： For the enzymatic determination of urea, one reacts the urea with glyoxylate in the presence of ureidoglycolate synthetase to give (S)-ureidoglycolate and oxidizes the latter with NAD(P).sup.+ and ureidoglycolate dehydrogenase to carbamoyloxamate and measures NAD(P)H formed either directly or via a color indicator system. A reagent suitable herefor contains glyoxylate, NAD(P).sup.+, (S)-ureidoglycolate synthetase, ureidoglycolate dehydrogenase and buffer substance.
摘要翻译： PCT No.PCT / EP85 / 00250 Sec。 371日期1986年1月10日 102（e）日期1986年1月10日PCT提交1985年5月23日PCT公布。出版物WO85 / 05641 1985年12月19日。尿素的酶法测定中，尿素与脲醛酸酯在脲基乙酸合成酶的存在下反应，得到（S） - 脲基乙醇酸酯，并用NAD（P）+和脲基乙酸脱氢酶将其氧化成氨基甲酰氨基甲酸酯 NAD（P）H直接或通过颜色指示器系统形成。适用于此的试剂含有乙醛酸，NAD（P）+，（S） - 脲基乙酸合成酶，脲基乙酸脱氢酶和缓冲物质。

30. 发明授权

US4705749A Process and reagent for the determination of glycerol with the use of glycerol kinase 失效
标题翻译：使用甘油激酶测定甘油的方法和试剂
公开(公告)号：US4705749A
公开(公告)日：1987-11-10
申请号：US667279
申请日：1984-11-01
申请人： Peter Willnow , Paul Lehmann , Joachim Ziegenhorn , August W. Wahlefeld
发明人： Peter Willnow , Paul Lehmann , Joachim Ziegenhorn , August W. Wahlefeld
IPC分类号： C12Q1/28 , C12Q1/44 , C12Q1/48 , C12N9/99 , C12Q1/26 , C12Q1/32
CPC分类号： C12Q1/44 , C12Q1/28 , C12Q1/48 , C12Q2326/96 , C12Q2334/00 , G01N2333/904 , G01N2333/9121 , Y10S435/805 , Y10S435/81
摘要： The present invention provides a process for the determination of glycerol in free or bound form by reaction with ATP in the presence of glycerol kinase (GK) and optionally of a hydrolase with the formation of glycerol-3-phosphate and ADP and determination of one of these reaction products with the help of at least one subsequent enzymatic reaction, wherein the determination is carried out kinetically and, for this purpose, the reaction with ATP is made rate-determining for the whole reaction and this is allowed to proceed according to the pseudo-first order in that there is added a sugar of the general formula: ##STR1## in which carbon atoms 2 and 3 have the D-threo configuration and R is a carbohydrate radical containing up to 3 carbon atoms and in which one hydroxyl group can also be replaced by a hydrogen atom.The present invention also provides a reagent for the kinetic determination of glycerol, comprising glycerol kinase, ATP and a system for the determination of glycerol-3-phosphate or of ADP and optionally a hydrolase, wherein it additionally contains a sugar of the general formula: ##STR2## in which carbon atoms 2 and 3 have the D-threo configuration and R is a carbohydrate radical containing up to 3 carbon atoms and in which one hydroxyl group can also be replaced by a hydrogen atom.
摘要翻译：本发明提供了一种通过在甘油激酶（GK）存在下与ATP反应来测定游离或结合形式的甘油的方法，并且任选地形成甘油-3-磷酸酯和ADP的水解酶，并测定其中之一这些反应产物借助于至少一个随后的酶反应，其中所述测定在动力学上进行，为此，与ATP的反应对整个反应进行速率确定，并且允许其根据伪添加了具有以下通式的糖：其中碳原子2和3具有D-苏糖构型并且R是含有至多3个碳原子的碳水化合物基团，其中一个羟基也可以被氢原子代替。本发明还提供用于动力学测定甘油的试剂，其包含甘油激酶，ATP和用于测定甘油-3-磷酸或ADP和任选的水解酶的系统，其中其还含有以下通式的糖：其中碳原子2和3具有D-苏结构，并且R是含有至多3个碳原子的碳水化合物基团，其中一个羟基也可被氢原子代替。

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