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21. 发明授权

US5656493A System for automated performance of the polymerase chain reaction 失效
标题翻译：用于自动化表现聚合酶链反应的系统
公开(公告)号：US5656493A
公开(公告)日：1997-08-12
申请号：US199505
申请日：1994-02-18
申请人： Kary B. Mullis , Larry Johnson , Richard A. Leath , Timothy J. Wennberg , Louis M. Mezei , Joseph T. Widunas
发明人： Kary B. Mullis , Larry Johnson , Richard A. Leath , Timothy J. Wennberg , Louis M. Mezei , Joseph T. Widunas
IPC分类号： B01J19/00 , B01L7/00 , C12Q1/68 , C40B40/06 , C40B60/14 , C12M3/02 , C12M1/00 , C12N15/00 , G01N21/00
CPC分类号： B01L7/52 , B01J19/0046 , C12Q1/686 , B01J2219/00495 , B01J2219/0059 , B01J2219/00689 , B01J2219/00722 , C40B40/06 , C40B60/14
摘要： There is disclosed herein a machine for performing nucleic acid amplification under computer control. The machine utilizes any one of a number of heating and cooling systems under control of a host computer which directs the heating and cooling systems to heat and cool a reaction-chamber-containing heat exchanger at appropriate times in the process. The reaction chambers are pre-loaded with the nucleic acid(s) to be amplified, a thermostable enzyme to catalyze polymerization, specific oligonucleotide primers, and four different nucleotide triphosphates. Also disclosed is the process for the amplification chain reaction implemented by the machine, which utilizes a thermostable enzyme.
摘要翻译：本文公开了一种用于在计算机控制下进行核酸扩增的机器。该机器在主机的控制下使用多个加热和冷却系统中的任何一个，其引导加热和冷却系统在该过程中适当的时间对含反应室的热交换器进行加热和冷却。反应室预先加载待扩增的核酸，催化聚合的热稳定酶，特异性寡核苷酸引物和四种不同的核苷酸三磷酸。还公开了由机器实现的扩增链反应的方法，其利用热稳定性酶。

22. 发明授权

US5028792A System for the visualization of exposure to ultraviolet radiation 失效
标题翻译：紫外线照射可视化系统
公开(公告)号：US5028792A
公开(公告)日：1991-07-02
申请号：US344274
申请日：1989-04-27
申请人： Kary B. Mullis
发明人： Kary B. Mullis
IPC分类号： G01J1/50 , G03C1/73
CPC分类号： G01J1/50 , G03C1/73
摘要： Photochemical systems for the direct visualization of exposure to ultraviolet radiation that effect visible color changes involving a process in which a photoacid is formed upon irradiation of a nitro-substituted aromatic aldehyde with ultraviolet light and wherein proton transfer to a dye causes the dye to undergo a visible color change. The system undergoes such color change to an extent directly proportional to the cumulative amount of ultraviolet incident thereupon.
摘要翻译：用于直接观察紫外线照射的光化学体系，其影响可见颜色变化，其涉及在用紫外线照射硝基取代的芳族醛时形成光致酸的方法，并且其中质子转移到染料使染料经历可见颜色变化。该系统经历与其入射的紫外线累积量成正比的变化。

23. 发明授权

US4803297A Carbamic acid ester useful for preparing a nucleic acid probe 失效
标题翻译：可用于制备核酸探针的氨基甲酸酯
公开(公告)号：US4803297A
公开(公告)日：1989-02-07
申请号：US72339
申请日：1987-07-13
申请人： Corey H. Levenson , Kary B. Mullis
发明人： Corey H. Levenson , Kary B. Mullis
IPC分类号： C07D493/04 , C07D513/04 , C07D519/00 , C07H21/00 , C07C125/06
CPC分类号： C07D493/04 , C07D513/04 , C07H21/00
摘要： Nucleic acids may be labeled by intercalating the alkylating intercalation moiety of a labeling reagent into a partially double-stranded nucleic acid to form a complex and activating the complex to cause covalent bonding between the reagent and the nucleic acid. Preferably, the labeled nucleic acid is hybridization probe for detecting nucleic acid sequences capable of hybridizing with a hybridizing region of the nucleic acid. Also preferably the label moiety is non-radioactive. The labeling reagent is of the formula:[A--[B--Lwhere A is an alkylating intercalation moiety, B is a divalent organic moiety of the formula: ##STR1## where Y is O, NH or N--CHO, x is a number from 1 to 4, y is a number from 2 to 4, and L is a monovalent label moiety, wherein B is exclusive of any portion of the intercalation and label moieties.Preferably A is a 4-methylene-substituted psoralen moiety, and most preferably A is a 4'-methylene-substituted-4,5',8-trimethylpsoralen moiety and L is biotin.
摘要翻译：可以通过将标记试剂的烷基化插层部分嵌入部分双链核酸中以形成复合物并激活复合物以引起试剂和核酸之间的共价键来标记核酸。优选地，标记的核酸是用于检测能够与核酸的杂交区杂交的核酸序列的杂交探针。还优选地，标记部分是非放射性的。标记试剂具有下式：[A- [BL，其中A是烷基化插层部分，B是下式的二价有机部分：其中Y是O，NH或N-CHO，x是从 1至4，y为2至4的数，L为单价标记部分，其中B不包括插层和标记部分的任何部分。优选A是4-亚甲基取代的补骨脂素部分，最优选A是4'-亚甲基取代的-4,5'，8-三甲基肉豆蔻酸酯部分，L是生物素。

24. 发明授权

US4751313A Precursor to nucleic acid probe 失效
标题翻译：核酸探针前体
公开(公告)号：US4751313A
公开(公告)日：1988-06-14
申请号：US72531
申请日：1987-07-13
申请人： Corey H. Levenson , Kary B. Mullis
发明人： Corey H. Levenson , Kary B. Mullis
IPC分类号： C07D493/04 , C07D513/04 , C07D519/00 , C07H21/00 , C07D495/04
CPC分类号： C07D493/04 , C07D513/04 , C07H21/00
摘要： Nucleic acids may be labeled by intercalating the alkylating intercalation moiety of a labeling reagent into a partially double-stranded nucleic acid to form a complex and activating the complex to cause covalent bonding between the reagent and the nucleic acid. Preferably, the labeled nucleic acid is a hybridization probe for detecting nucleic acid sequences capable of hybridizing with a hybridizing region of the nucleic acid. Also preferably the label moiety is non-radioactive. The labeling reagent is of the formula:[A][B]Lwhere A is an alkylating intercalation moiety, B is a divalent organic moiety of the formula: ##STR1## where Y is O, NH or N--CHO, x is a number from 1 to 4, y is a number from 2 to 4, and L is a monovalent label moiety, wherein B is exclusive of any portion of the intercalation and label moieties.Preferably A is a 4-methylene-substituted psoralen moiety, and most preferably A is a 4'-methylene-substituted-4,5',8-trimethylpsoralen moeity and L is biotin.
摘要翻译：可以通过将标记试剂的烷基化插层部分嵌入部分双链核酸中以形成复合物并激活复合物以引起试剂和核酸之间的共价键来标记核酸。优选地，标记的核酸是用于检测能够与核酸的杂交区域杂交的核酸序列的杂交探针。还优选地，标记部分是非放射性的。标记试剂具有下式：[A] [B] L，其中A是烷基化插层部分，B是下式的二价有机部分：其中Y是O，NH或N-CHO，x是 1至4的数，y为2至4的数，L为单价标记部分，其中B不包括插入和标记部分的任何部分。优选A是4-亚甲基取代的补骨脂素部分，最优选A是4'-亚甲基取代的-4,5'，8-三甲基肉桂酸酯，L是生物素。

25. 发明授权

US08591910B2 Chemically programmable immunity 有权
公开(公告)号：US08591910B2
公开(公告)日：2013-11-26
申请号：US13543718
申请日：2012-07-06
申请人： Kary B. Mullis
发明人： Kary B. Mullis
IPC分类号： A61K38/14 , A61K39/00 , A61K39/385 , C07K9/00 , C07H21/04 , C12N15/115
CPC分类号： A61K39/385 , A61K2039/6025 , Y02A50/386
摘要： The present invention is related to methods and compositions that are capable of immediately immunizing a human or animal against any molecule or compound. The present invention comprises an immunity linker molecule with at least two sites; (1) a first binding site that binds to an immune system molecule in a human or animal that has been preimmunized against the first binding site, and (2) one or more second binding sites that bind specifically to a desired compound or molecule. The first binding site and the second binding site(s) are linked by a linker portion of the molecule.

26. 发明申请

US20090142368A1 Chemically Programmable Immunity 有权
标题翻译：化学可编程抗扰度
公开(公告)号：US20090142368A1
公开(公告)日：2009-06-04
申请号：US12336746
申请日：2008-12-17
申请人： Kary B. Mullis
发明人： Kary B. Mullis
IPC分类号： A61K39/00
CPC分类号： A61K39/385 , A61K2039/6025 , Y02A50/386
摘要： The present invention is related to methods and compositions that are capable of immediately immunizing a human or animal against any molecule or compound. The present invention comprises an immunity linker molecule with at least two sites; (1) a first binding site that binds to an immune system molecule in a human or animal that has been preimmunized against the first binding site, and (2) one or more second binding sites that bind specifically to a desired compound or molecule. The first binding site and the second binding site(s) are linked by a linker portion of the molecule.
摘要翻译：本发明涉及能够立即针对任何分子或化合物免疫人或动物的方法和组合物。本发明包括具有至少两个位点的免疫接头分子; （1）结合已针对第一结合位点预免疫的人或动物中的免疫系统分子的第一结合位点，和（2）特异性结合所需化合物或分子的一个或多个第二结合位点。第一结合位点和第二结合位点通过分子的接头部分连接。

27. 发明授权

US06514736B1 Kits for amplifying and detecting nucleic acid sequences 失效
标题翻译：用于扩增和检测核酸序列的试剂盒
公开(公告)号：US06514736B1
公开(公告)日：2003-02-04
申请号：US09704357
申请日：2000-11-01
申请人： Henry A. Erlich , Glenn Horn , Randall K. Saiki , Kary B. Mullis , David H. Gelfand
发明人： Henry A. Erlich , Glenn Horn , Randall K. Saiki , Kary B. Mullis , David H. Gelfand
IPC分类号： C12N912
CPC分类号： C12Q1/6858 , B01J19/0046 , B01J2219/0059 , B01J2219/00722 , B01L7/52 , C07K14/70539 , C07K14/805 , C12N9/1252 , C12N15/10 , C12P19/34 , C12Q1/6827 , C12Q1/6853 , C12Q1/686 , C40B40/06 , Y10S977/92 , C12Q2549/119 , C12Q2525/149 , C12Q2525/143 , C12Q2525/131
摘要： The present invention is directed to a process for amplifying any target nucleic acid sequence contained in a nucleic acid or mixture thereof using a thermostable enzyme. The process comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers, extending the primers with a thermostable enzyme to form complementary primer extension products which act as templates for synthesizing the desired nucleic acid sequence, and detecting the sequence so amplified. The steps of the reaction can be repeated as often as desired and involve temperature cycling to effect hybridization, promotion of activity of the enzyme, and denaturation of the hybrids formed.
摘要翻译：本发明涉及使用热稳定性酶扩增核酸或其混合物中包含的任何靶核酸序列的方法。该方法包括用摩尔过量的两种寡核苷酸引物处理核酸的分开的互补链，用热稳定酶扩增引物以形成互补引物延伸产物，其用作合成所需核酸序列的模板，并检测序列放大反应的步骤可以根据需要经常重复，并且涉及温度循环以进行杂交，促进酶的活性和所形成的杂交体的变性。

28. 发明授权

US6040166A Kits for amplifying and detecting nucleic acid sequences, including a probe 失效
标题翻译：用于扩增和检测核酸序列的试剂盒，包括探针
公开(公告)号：US6040166A
公开(公告)日：2000-03-21
申请号：US312729
申请日：1994-09-27
申请人： Henry A. Erlich , Glenn Horn , Randall K. Saiki , Kary B. Mullis , David H. Gelfand
发明人： Henry A. Erlich , Glenn Horn , Randall K. Saiki , Kary B. Mullis , David H. Gelfand
IPC分类号： B01J19/00 , B01L7/00 , C07K14/74 , C07K14/805 , C12N9/12 , C12N15/10 , C12P19/34 , C12Q1/68 , C40B40/06
CPC分类号： C12Q1/6858 , B01J19/0046 , B01L7/52 , C07K14/70539 , C07K14/805 , C12N15/10 , C12N9/1252 , C12P19/34 , C12Q1/6827 , C12Q1/6853 , C12Q1/686 , B01J2219/0059 , B01J2219/00722 , C40B40/06 , Y10S977/92
摘要： The present invention is directed to a process for amplifying any target nucleic acid sequence contained in a nucleic acid or mixture thereof using a thermostable enzyme. The process comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers, extending the primers with a thermostable enzyme to form complementary primer extension products which act as templates for synthesizing the desired nucleic acid sequence, and detecting the sequence so amplified. The steps of the reaction can be repeated as often as desired and involve temperature cycling to effect hybridization, promotion of activity of the enzyme, and denaturation of the hybrids formed.
摘要翻译：本发明涉及使用热稳定性酶扩增核酸或其混合物中包含的任何靶核酸序列的方法。该方法包括用摩尔过量的两种寡核苷酸引物处理核酸的分开的互补链，用热稳定酶扩增引物以形成互补引物延伸产物，其用作合成所需核酸序列的模板，并检测序列放大反应的步骤可以根据需要经常重复，并且涉及温度循环以进行杂交，促进酶的活性和所形成的杂交体的变性。

29. 发明授权

US5604099A Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids 失效
标题翻译：用于检测核酸中存在的特异性核苷酸变异和遗传多态性的方法
公开(公告)号：US5604099A
公开(公告)日：1997-02-18
申请号：US457647
申请日：1995-06-01
申请人： Henry A. Erlich , Glenn Horn , Randall K. Saiki , Kary B. Mullis
发明人： Henry A. Erlich , Glenn Horn , Randall K. Saiki , Kary B. Mullis
IPC分类号： C07K14/74 , C12Q1/68 , C12N9/12 , C12N15/11 , C12P19/34
CPC分类号： C12Q1/6881 , C07K14/70539 , C12Q1/6827 , C12Q2600/156 , C12Q2600/172
摘要： Single or multiple nucleotide variations in nucleic acid sequence can be detected in nucleic acids by a process whereby the sample suspected of containing the relevant nucleic acid is repeatedly treated with primers, nucleotide triphosphates, and an agent for polymerization of the triphosphates and then denatured, in a process which amplifies the sequence containing the nucleotide variation if it is present. In one embodiment, the sample is spotted on a membrane and treated with a labeled sequence-specific oligonucleotide probe. Hybridization of the probe to the sample is detected by the label on the probe.
摘要翻译：核酸序列中的单个或多个核苷酸变异可通过以下过程在核酸中检测，其中怀疑含有相关核酸的样品用引物，三磷酸核苷酸和用于聚合三磷酸酯的试剂重复处理，然后变性如果存在，则扩增含有核苷酸变异的序列的过程。在一个实施方案中，将样品点在膜上并用标记的序列特异性寡核苷酸探针处理。通过探头上的标签检测探针与样品的杂交。

30. 发明授权

US5436115A Systems for the visualization of exposure to ultraviolet radiation 失效
标题翻译：暴露于紫外线辐射的可视化系统
公开(公告)号：US5436115A
公开(公告)日：1995-07-25
申请号：US71933
申请日：1993-06-02
申请人： Kary B. Mullis
发明人： Kary B. Mullis
IPC分类号： G01J1/50 , G03C1/73
CPC分类号： G01J1/50 , G03C1/73
摘要： Photochemical systems for the direct visualization of exposure to ultraviolet radiation that effect visible color changes involving a process in which a photoacid is formed upon irradiation of a nitro-substituted aromatic aldehyde with ultraviolet light and wherein proton transfer to a dye causes the dye to undergo a visible color change. The system undergoes such color change to an extent directly proportional to the cumulative amount of ultraviolet incident thereupon.
摘要翻译：用于直接观察紫外线照射的光化学体系，其影响可见颜色变化，其涉及在用紫外线照射硝基取代的芳族醛时形成光致酸的方法，并且其中质子转移到染料使染料经历可见颜色变化。该系统经历与其入射的紫外线累积量成正比的变化。

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