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21. 发明申请

US20140017749A1 Processes for Producing Fermentation Products 有权
标题翻译：生产发酵产品的工艺
公开(公告)号：US20140017749A1
公开(公告)日：2014-01-16
申请号：US13994310
申请日：2011-12-21
申请人： Randy Deinhammer , Suzanne Clark , Mauricio Quiros , John Matthews , Anne Glud Hjulmand , Chee Leong Soong , Tomoko Matsui , Shinobu Takagi
发明人： Randy Deinhammer , Suzanne Clark , Mauricio Quiros , John Matthews , Anne Glud Hjulmand , Chee Leong Soong , Tomoko Matsui , Shinobu Takagi
IPC分类号： C12P7/06
CPC分类号： C12P7/06 , C12P19/02 , C12P19/14 , C12P19/20 , C12Y302/01001 , C12Y302/01003 , C12Y304/24 , Y02E50/17
摘要： The present invention relates to processes for producing fermentation products from starch-containing material, wherein a thermostable alpha-amylase and optionally a thermostable protease are present and/or added during liquefaction. The invention also relates to a composition suitable for use in a process of the invention.
摘要翻译：本发明涉及用于从含淀粉材料生产发酵产物的方法，其中在液化期间存在和/或加入热稳定性α-淀粉酶和任选的热稳定蛋白酶。本发明还涉及适用于本发明方法的组合物。

22. 发明申请

US20120225468A1 Hafnia Phytase Variants 有权
标题翻译： Hafnia植酸酶变体
公开(公告)号：US20120225468A1
公开(公告)日：2012-09-06
申请号：US13472822
申请日：2012-05-16
申请人： Soeren Flensted Lassen , Leonardo De Maria , Esben Peter Friis , Tomoko Matsui , Allan Noergaard , Lars Kobberoee Skov , Jesper Vind
发明人： Soeren Flensted Lassen , Leonardo De Maria , Esben Peter Friis , Tomoko Matsui , Allan Noergaard , Lars Kobberoee Skov , Jesper Vind
IPC分类号： C12N9/16
CPC分类号： C12N9/16 , A23K20/189 , A23K50/30 , A23K50/75 , Y02E50/17
摘要： The present invention relates to phytases having at least 76% identity to a phytase derived from Hafnia alvei and comprises at least one modification in the amino acid sequence thereof. These phytase variants have modified, preferably improved, properties, such as, reduced protease sensibility, preferably they exhibit improved properties in respect of thermal performance, such as heat-stability (temperature stability, thermostability), steam stability, pelleting stability and/or temperature profile; and/or protease stability, in particular pepsin stability, pH profile, specific activity, substrate specificity, performance in animal feed (such as an improved release and/or degradation of phytate), susceptibility to glycation, and/or glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g., in animal feed and animal feed additives.
摘要翻译：本发明涉及与衍生自哈夫单胞菌属植物的植酸酶具有至少76％同一性的植酸酶，并且包含其氨基酸序列中的至少一个修饰。这些植酸酶变体具有改性的，优选改进的性质，例如降低的蛋白酶敏感性，优选它们在热性能方面表现出改进的性能，例如热稳定性（温度稳定性，热稳定性），蒸汽稳定性，造粒稳定性和/或温度简介和/或蛋白酶稳定性，特别是胃蛋白酶稳定性，pH曲线，比活性，底物特异性，动物饲料中的性能（如植酸盐的改善的释放和/或降解），对糖基化的敏感性和/或糖基化模式。本发明还涉及编码这些植酸酶的DNA，其生产方法及其用途，例如在动物饲料和动物饲料添加剂中。

23. 发明授权

US08206962B2 Hafnia phytase variants 有权
标题翻译： Hafnia植酸酶变体
公开(公告)号：US08206962B2
公开(公告)日：2012-06-26
申请号：US12568312
申请日：2009-09-28
申请人： Soeren Flensted Lassen , Leonardo De Maria , Esben Peter Friis , Tomoko Matsui , Allan Noergaard , Lars Kobberoee Skov , Jesper Vind
发明人： Soeren Flensted Lassen , Leonardo De Maria , Esben Peter Friis , Tomoko Matsui , Allan Noergaard , Lars Kobberoee Skov , Jesper Vind
IPC分类号： C12N9/14 , C12Q1/34 , C07H21/04
CPC分类号： C12N9/16 , A23K20/189 , A23K50/30 , A23K50/75 , Y02E50/17
摘要： The present invention relates to phytases having at least 76% identity to a phytase derived from Hafnia alvei and comprises at least one modification in the amino acid sequence thereof. These phytase variants have modified, preferably improved, properties, such as, reduced protease sensibility, preferably they exhibit improved properties in respect of thermal performance, such as heat-stability (temperature stability, thermostability), steam stability, pelleting stability and/or temperature profile; and/or protease stability, in particular pepsin stability, pH profile, specific activity, substrate specificity, performance in animal feed (such as an improved release and/or degradation of phytate), susceptibility to glycation, and/or glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g., in animal feed and animal feed additives.
摘要翻译：本发明涉及与衍生自哈夫单胞菌属植物的植酸酶具有至少76％同一性的植酸酶，并且包含其氨基酸序列中的至少一个修饰。这些植酸酶变体具有改性的，优选改进的性质，例如降低的蛋白酶敏感性，优选它们在热性能方面表现出改进的性能，例如热稳定性（温度稳定性，热稳定性），蒸汽稳定性，造粒稳定性和/或温度简介和/或蛋白酶稳定性，特别是胃蛋白酶稳定性，pH曲线，比活性，底物特异性，动物饲料中的性能（如植酸盐的改善的释放和/或降解），对糖基化的敏感性和/或糖基化模式。本发明还涉及编码这些植酸酶的DNA，其生产方法及其用途，例如在动物饲料和动物饲料添加剂中。

24. 发明授权

US08124379B2 Signal peptide for producing a polypeptide 有权
标题翻译：产生多肽的信号肽
公开(公告)号：US08124379B2
公开(公告)日：2012-02-28
申请号：US12415174
申请日：2009-03-31
申请人： Tomoko Matsui , Henriette Draborg , Steffen Danielsen
发明人： Tomoko Matsui , Henriette Draborg , Steffen Danielsen
IPC分类号： C12N15/62 , C12N15/80 , C12N15/81
CPC分类号： C12N9/6472 , C07K14/37 , C12N9/16 , C12N9/2437 , C12N9/6424 , C12N15/625
摘要： The present invention relates to a method for producing a polypeptide comprising using a signal peptide, to nucleic acid constructs comprising a first nucleotide sequence encoding the signal peptide and a second nucleotide sequence encoding a polypeptide which is foreign to the first nucleotide sequence. Furthermore, it also relates to expression vectors and host cells comprising the nuclei acid construct.
摘要翻译：本发明涉及包含使用信号肽的多肽的制备方法，其包含编码信号肽的第一核苷酸序列的核酸构建体和编码第一核苷酸序列异源的多肽的第二核苷酸序列。此外，它还涉及包含核酸构建体的表达载体和宿主细胞。

25. 发明申请

US20100180095A1 BUFFER CONTROL DEVICE AND BUFFER MEMORY DEVICE 审中-公开
标题翻译：缓冲器控制器件和缓冲器存储器件
公开(公告)号：US20100180095A1
公开(公告)日：2010-07-15
申请号：US12095610
申请日：2006-11-28
申请人： Masanori Fujibayashi , Nobuo Higaki , Kazushi Kurata , Tomoko Matsui
发明人： Masanori Fujibayashi , Nobuo Higaki , Kazushi Kurata , Tomoko Matsui
IPC分类号： G06F12/14
CPC分类号： G06F5/14 , G06F2205/062
摘要： The buffer control device of this invention includes: a pointer holding unit which holds a virtual pointer different from a read pointer and a write pointer; an access control unit that controls an access to a ring buffer; a judging unit that judges whether or not one of the read pointer and the write pointer has reached an address substantially identical to an address indicated by the virtual pointer; and disabling unit that disables a normal access using the one of the read pointer and the write pointer, when the judging unit judges that the one of the read pointer and the write pointer has reached the address substantially identical to the address indicated by the virtual pointer, the normal access being controlled by the access control unit, wherein the access control unit further controls a reaccess to the ring buffer.
摘要翻译：本发明的缓冲器控制装置包括：保持与读指针不同的虚拟指针和写指针的指针保持单元; 访问控制单元，其控制对环形缓冲器的访问; 判断单元，判断读指针和写指针之一是否达到与虚拟指针所指示的地址基本相同的地址; 以及禁止单元，其在所述读取指针和所述写入指针之一达到与所述虚拟指针所指示的地址基本相同的地址的情况下，使用所述读取指针和所述写入指针中的一者禁止正常访问正常访问由访问控制单元控制，其中访问控制单元进一步控制对环形缓冲器的重新访问。

26. 发明授权

US07666652B2 Asparaginases 有权
标题翻译：天冬酰胺酶
公开(公告)号：US07666652B2
公开(公告)日：2010-02-23
申请号：US12044055
申请日：2008-03-07
申请人： Tomoko Matsui , Esben Peter Friis , Akihiko Yamagishi
发明人： Tomoko Matsui , Esben Peter Friis , Akihiko Yamagishi
IPC分类号： C12N9/82 , C07H21/04 , C12N1/20 , A21D2/00
CPC分类号： C12N9/82 , A23L5/25 , A23L19/18 , C12Y305/01001
摘要： The invention relates to new asparaginases having improved properties, preferably improved thermotolerance, such as improved activity at high temperatures and/or improved thermostability. The invention also relates to DNA sequences encoding such improved asparaginases, their production in a recombinant host cell, as well as methods of using the asparaginases, in particular for reduction of acrylamide in foods. The invention furthermore relates to methods of generating and preparing asparaginase variants having improved properties.
摘要翻译：本发明涉及具有改进性能的新型天冬酰胺酶，优选改进的耐热性，例如在高温下改善的活性和/或改善的热稳定性。本发明还涉及编码这种改良天冬酰胺酶的DNA序列，其在重组宿主细胞中的产生，以及使用天冬酰胺酶，特别是用于还原食品中丙烯酰胺的方法。本发明还涉及产生和制备具有改进性质的天冬酰胺酶变体的方法。

27. 发明申请

US20090170157A1 Asparaginases 有权
标题翻译：天冬酰胺酶
公开(公告)号：US20090170157A1
公开(公告)日：2009-07-02
申请号：US12044055
申请日：2008-03-07
申请人： Tomoko Matsui , Esben Peter Friis , Akihiko Yamagishi
发明人： Tomoko Matsui , Esben Peter Friis , Akihiko Yamagishi
IPC分类号： C12P21/00 , C12N9/48 , C07H21/04 , C12N15/74 , C12N1/00 , C12P1/00
CPC分类号： C12N9/82 , A23L5/25 , A23L19/18 , C12Y305/01001
摘要： The invention relates to new asparaginases having improved properties, preferably improved thermotolerance, such as improved activity at high temperatures and/or improved thermostability. The invention also relates to DNA sequences encoding such improved asparaginases, their production in a recombinant host cell, as well as methods of using the asparaginases, in particular for reduction of acrylamide in foods. The invention furthermore relates to methods of generating and preparing asparaginase variants having improved properties.
摘要翻译：本发明涉及具有改进性能的新型天冬酰胺酶，优选改进的耐热性，例如在高温下改善的活性和/或改善的热稳定性。本发明还涉及编码这种改良天冬酰胺酶的DNA序列，其在重组宿主细胞中的产生，以及使用天冬酰胺酶，特别是用于还原食品中丙烯酰胺的方法。本发明还涉及产生和制备具有改进性质的天冬酰胺酶变体的方法。

28. 发明授权

US09816112B2 Processes for producing fermentation products 有权
公开(公告)号：US09816112B2
公开(公告)日：2017-11-14
申请号：US13994310
申请日：2011-12-21
申请人： Randy Deinhammer , Suzanne Clark , Mauricio Quiros , John Matthews , Anne Glud Hjulmand , Chee Leong Soong , Tomoko Matsui , Shinobu Takagi
发明人： Randy Deinhammer , Suzanne Clark , Mauricio Quiros , John Matthews , Anne Glud Hjulmand , Chee Leong Soong , Tomoko Matsui , Shinobu Takagi
IPC分类号： C12P7/06 , C12P19/02 , C12P19/14 , C12P19/20
CPC分类号： C12P7/06 , C12P19/02 , C12P19/14 , C12P19/20 , C12Y302/01001 , C12Y302/01003 , C12Y304/24 , Y02E50/17
摘要： The present invention relates to processes for producing fermentation products from starch-containing material, wherein a thermostable alpha-amylase and optionally a thermostable protease are present and/or added during liquefaction. The invention also relates to a composition suitable for use in a process of the invention.

29. 发明授权

US09040280B2 Protease variants 有权
标题翻译：蛋白酶变体
公开(公告)号：US09040280B2
公开(公告)日：2015-05-26
申请号：US13510076
申请日：2010-12-10
申请人： Tomoko Matsui , Allan Noergaard , Thomas Agersten Poulsen , John Matthews
发明人： Tomoko Matsui , Allan Noergaard , Thomas Agersten Poulsen , John Matthews
IPC分类号： C12N9/48 , C12N9/58
CPC分类号： C12N9/58 , C12P7/06 , C12Y304/24 , Y02E50/16 , Y02E50/17
摘要： The present invention relates to proteases having at least 75% identity to a protease derived from Thermoascus aurantiacus and comprises at least one modification in the amino acid sequence thereof. These protease variants have improved thermostability. The invention also relates to DNA encoding these proteases, methods of their production, as well as the use thereof.
摘要翻译：本发明涉及与衍生自嗜热嗜铬鱼的蛋白酶具有至少75％同一性的蛋白酶，并且包含其氨基酸序列中的至少一个修饰。这些蛋白酶变体具有改善的热稳定性。本发明还涉及编码这些蛋白酶的DNA，其生产方法及其用途。

30. 发明申请

US20130260410A1 MICROPLATE SAMPLING ADAPTER 审中-公开
标题翻译：麦克风采样适配器
公开(公告)号：US20130260410A1
公开(公告)日：2013-10-03
申请号：US13992541
申请日：2011-12-08
申请人： Annette Helle Johansen , Jakob Rothe , Noriko Tsutsumi , Tomoko Matsui
发明人： Annette Helle Johansen , Jakob Rothe , Noriko Tsutsumi , Tomoko Matsui
IPC分类号： C12Q1/24
CPC分类号： C12Q1/24 , B01L3/5085 , B01L3/50853 , B01L3/563 , B01L2300/046 , B01L2300/0829 , C12M33/06 , C12M33/12
摘要： The present invention relates to adapters for microplates. The adapters allow sampling from a liquid growth medium in a microplate well after a microorganism has formed a dense layer of biomass on the surface of the growth medium. The adapters also allow easy transfer of a surface-grown microorganism from all wells of a microplate in one go, when inserted before the microorganism form the layer and then removed after incubation and layer-formation, which also enables easy sampling of the growth medium in the
摘要翻译：本发明涉及微孔板适配器。在微生物在生长培养基的表面上形成致密的生物质层之后，适配器允许从微孔板中的液体生长培养基中取样。适配器还允许在微生物形成层之前插入时，容易地从微板的所有孔转移表面生长的微生物，然后在孵育和层形成之后移除，这也使得能够容易地对生长培养基进行取样的

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