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    • 21. 发明授权
    • Autoradiographic gene-screening method
    • 放射自显影基因筛选方法
    • US4865967A
    • 1989-09-12
    • US127605
    • 1987-11-30
    • Hisashi ShiraishiJunji MiyaharaHisatoyo Kato
    • Hisashi ShiraishiJunji MiyaharaHisatoyo Kato
    • G01N33/50C12N15/09C12Q1/68G01N33/58G01N33/60G01T1/29
    • G01T1/2942C12Q1/6841
    • An autoradiographic gene-screening method employing a hybridization process, which comprises:(1) a step of transferring a portion of genetic clones cultured on a culture medium onto a filter to fix them thereonto;(2) a step of hybridizing the genes of said clones fixed onto said filter with radioactively labeled probes;(3) a step of obtaining two dimensional information on the location of the radioactively labeled substances on the filter which comprises placing said filter having been subjected to the hybridization and a stimulable phosphor sheet in layers for a given period of time to cause said stimulable phosphor sheet to absorb at least a portion of radiation energy emitted by the radioactively labeled substances on the filter, exciting said sheet with an electromagnetic wave to release the radiation energy stored in said sheet as stimulated emission, and detecting the stimulated emission to obtain a locational information on the radioactively labeled substances on the filter; and(4) a step recovering the clones on the culture medium according to the obtained locational information.
    • 使用杂交方法的放射自显影基因筛选方法,其包括:(1)将在培养基上培养的一部分遗传克隆转移到过滤器上以将其固定在其上的步骤; (2)将固定在所述过滤器上的所述克隆的基因与放射性标记的探针杂交的步骤; (3)获得关于过滤器上的放射性标记物质的位置的二维信息的步骤,该步骤包括将已经进行了杂交的所述过滤器和可刺激的磷光体片层压一段给定的时间以引起所述可刺激的荧光体 以吸收由过滤器上的放射性标记的物质发射的辐射能量的至少一部分,用电磁波激发所述片材,以释放作为受激发射存储在所述片材中的辐射能量,并且检测受激发射以获得位置信息 在过滤器上的放射性标记物质上; 和(4)根据获得的位置信息恢复培养基上的克隆的步骤。
    • 25. 发明授权
    • Method of detecting radioactive substance
    • 检测放射性物质的方法
    • US4956559A
    • 1990-09-11
    • US6925
    • 1987-01-27
    • Hisashi Shiraishi
    • Hisashi Shiraishi
    • G01N30/84G01T1/29G01T7/04
    • G01T7/04G01T1/2012G01T1/2014G01N2030/77G01N2030/8417
    • A method of detecting radioactive substance in a liquid sample which comprises steps of:(1) supplying a radiation-measuring instrument containing a stimulable phosphor with the liquid sample continuously or intermittently;(2) keeping said measuring instrument in contact with the liquid sample for a given period of time to cause the instrument to absorb at least a portion of radiation energy emitted by the radioactive substance in said liquid sample; and(3) irradiating said measuring instrument with an electromagnetic wave to release the radiation energy stored in the instrument as stimulated emission, and photoelectrically detecting the stimulated emission to measure radioactivity of said liquid sample sequentially.The method is particularly useful when employed in conjunction with liquid chromatography.
    • 一种检测液体样品中的放射性物质的方法,包括以下步骤:(1)连续或间歇地向液体样品供应含有可刺激的荧光体的辐射测量仪器; (2)使所述测量仪器与所述液体样品接触一段给定的时间,以使所述仪器吸收由所述液体样品中的放射性物质发射的辐射能量的至少一部分; 和(3)用电磁波照射所述测量仪器以释放作为受激发射存储在仪器中的辐射能量,并且光电检测受激发射以依次测量所述液体样品的放射性。 当与液相色谱联合使用时,该方法特别有用。