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21. 发明授权

EP2806754B1 DITERPENE PRODUCTION 无效
公开(公告)号：EP2806754B1
公开(公告)日：2018-10-24
申请号：EP13700929.6
申请日：2013-01-23
申请人： DSM IP Assets B.V.
发明人： KUMAR, Manoj , VAN DEN BERG, Marco Alexander , BOER, Viktor Marius , LAWRENCE, Adam G. , LEHMANN, Martin , MEIJRINK, Bernard , SAGT, Cornelis Maria Jacobus , TRUEHART, Joshua , WU, Liang , ZWARTJENS, Priscilla
IPC分类号： C12N9/02 , C12N9/10 , C12N9/90 , A23L2/60 , C12P5/00 , C07H15/256 , C12P19/56 , C12N15/52 , A23K20/121 , A23K20/163 , A23K20/10 , A23L27/30 , C12N9/88
CPC分类号： C12P19/56 , A23K20/10 , A23K20/121 , A23K20/163 , A23L2/60 , A23L27/36 , A23V2002/00 , C07H15/256 , C12N9/0073 , C12N9/1051 , C12N9/88 , C12N9/90 , C12N15/52 , C12P5/007 , C12Y114/13078 , C12Y204/01035 , C12Y402/03019 , C12Y505/01013
摘要： The present invention relates to a recombinant microorganism comprising one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity, whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol. The recombinant microorganism may also be capable of expressing one or more UDP-glucosyltransferases such that the microorganism is capable of producing one or more steviol glycosides.

22. 发明公开

EP3242950A1 A CRISPR-CAS SYSTEM FOR A FILAMENTOUS FUNGAL HOST CELL 有权
标题翻译：用于丝状真菌宿主细胞的CRISPR-CAS系统
公开(公告)号：EP3242950A1
公开(公告)日：2017-11-15
申请号：EP16716466.4
申请日：2016-01-06
申请人： DSM IP Assets B.V. , Rijksuniversiteit Groningen
发明人： MEIJRINK, Bernard , VERWAAL, René , GIELESEN, Bianca Elisabeth Maria , ROUBOS, Johannes Andries , NYGÅRD, Yvonne Irene , BOVENBERG, Roelof Arij Lans , DRIESSEN, Arnold Jacob Mathieu , POHL, Carsten
IPC分类号： C12N15/90
CPC分类号： C12N15/902 , C12N15/102 , C12N15/111 , C12N15/905 , C12N2310/10 , C12N2310/20
摘要： The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a filamentous fungal host cell.

23. 发明公开

EP2922952A2 ITACONIC ACID AND ITACONATE METHYLESTER PRODUCTION 审中-公开
标题翻译：意大利酸和意大利甲醇生产
公开(公告)号：EP2922952A2
公开(公告)日：2015-09-30
申请号：EP13795497.0
申请日：2013-11-25
申请人： DSM IP Assets B.V.
发明人： ZHAO, Zheng , MEIJRINK, Bernard , HOEVEN, VAN DER, Robertus Antonius Mijndert , WU, Liang , ROUBOS, Johannes, Andries
IPC分类号： C12N15/10 , C12N15/52 , C40B40/06 , C40B40/08 , C12P7/46
CPC分类号： C12P7/46 , C12N9/88 , C12N15/81 , C12P7/62 , C12Y401/01006
摘要： The present invention relates to a recombinant yeast cell which is capable of producing one or more of 4-methyl itaconate or 1-methyl itaconate. The invention also relates to a recombinant yeast cell which is capable of producing itaconic acid and which overexpresses: a nucleic acid encoding a polypeptide having cis-aconitate decarboxylase activity; and a nucleic acid encoding a polypeptide which catalyzes a reaction towards acetyl CoA. These recombinant yeast cells may be used in processes for the production of itaconic acid, 4-methyl itaconate or 1-methyl itaconate.
摘要翻译：本发明涉及能够产生衣康酸4-甲酯或衣康酸1-甲酯中的一种或多种的重组酵母细胞。本发明还涉及能够产生衣康酸并且过表达编码具有顺乌头酸脱羧酶活性的多肽的核酸的重组酵母细胞; 和编码催化对乙酰辅酶A的反应的多肽的核酸。这些重组酵母细胞可用于生产衣康酸，衣康酸4-甲酯或衣康酸1-甲酯的方法中。

24. 发明授权

EP2831238B1 CLONING METHOD 有权
标题翻译：克隆方法
公开(公告)号：EP2831238B1
公开(公告)日：2018-01-03
申请号：EP13716982.7
申请日：2013-03-27
申请人： DSM IP Assets B.V.
发明人： ROUBOS, Johannes, Andries , PEL, Herman Jan , MEIJRINK, Bernard
IPC分类号： C12N15/66 , C12N15/90
CPC分类号： C12N15/1024 , C12N15/66 , C12N15/90 , C12N2800/30 , C12N2800/40 , C12N2800/50 , C12N2800/80
摘要： The present invention relates to a method based on the use of restriction enzyme digestion and ligation via cleavage sites, thereby to prepare two or more standardized expression cassettes.

25. 发明公开

EP3242949A1 A CRISPR-CAS SYSTEM FOR A YEAST HOST CELL 有权
标题翻译：用于酵母宿主细胞的CRISPR-CAS系统
公开(公告)号：EP3242949A1
公开(公告)日：2017-11-15
申请号：EP16700158.5
申请日：2016-01-06
申请人： DSM IP Assets B.V.
发明人： VERWAAL, René , MEIJRINK, Bernard , WIESSENHAAN, Nathalie , VONK, Brenda , ROUBOS, Johannes Andries
IPC分类号： C12N15/90
CPC分类号： C12N15/905 , C12N9/22 , C12N15/102 , C12N15/113 , C12N15/81 , C12N15/902 , C12N2310/20 , C12P23/00
摘要： The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a yeast host cell.

26. 发明公开

EP3242948A1 A CRISPR-CAS SYSTEM FOR A LIPOLYTIC YEAST HOST CELL 有权
标题翻译：一个用于脂质酵母宿主细胞的CRISPR-CAS系统
公开(公告)号：EP3242948A1
公开(公告)日：2017-11-15
申请号：EP16700157.7
申请日：2016-01-06
申请人： DSM IP Assets B.V.
发明人： MEIJRINK, Bernard , VERWAAL, René , GIELESEN, Bianca Elisabeth Maria , ROUBOS, Johannes Andries
IPC分类号： C12N15/90 , C12N15/63
CPC分类号： C12N15/905 , C12N9/22 , C12N15/11 , C12N15/63 , C12N2310/20
摘要： The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a lipolytic yeast host cell.

27. 发明公开

EP2898076A1 CELL MODIFICATION METHOD USING ESSENTIAL GENES AS MARKERS AND OPTIONALLY RECYCLING THESE 失效
标题翻译： ZELLMODIFIZIERUNGSVERFAHREN UNTER VERWENDUNG VON ESSENTIELLEN GENAL ALS MARKER UND OPTIONALEM RECYCLING DIESER
公开(公告)号：EP2898076A1
公开(公告)日：2015-07-29
申请号：EP13763111.5
申请日：2013-09-19
申请人： DSM IP Assets B.V.
发明人： LANGE, DE, Ilse , MEIJRINK, Bernard , ROUBOS, Johannes, Andries , OOI, Siew-loon
IPC分类号： C12N15/65 , C12N15/80
CPC分类号： C12N15/65 , C12N15/80 , C12N15/81
摘要： The invention relates to a method for modification of a host cell at a target locus, which method comprises: providing a host cell comprising, at a first locus, at least two site-specific recombination sites and a nucleic acid having an essential function or encoding a product having an essential function; introducing into the host cell, at the target locus, a further nucleic acid having the essential function or encoding for a product having the essential function; and carrying out recombination at the first locus via the at least two site-specific recombination sites, so that the nucleic acid having an essential function or encoding a product having an essential function is rendered non-functional, thereby to modify the host cell at the target locus. The invention also relates to a cell obtainable by a method of the invention.
摘要翻译：本发明涉及用于在靶标位点修饰宿主细胞的方法，该方法包括：提供宿主细胞，其在第一场所包含至少两个位点特异性重组位点和具有基本功能或编码的核酸具有基本功能的产品; 在目标基因座的宿主细胞中引入具有基本功能或编码具有基本功能的产品的另外的核酸; 并通过至少两个位点特异性重组位点在第一位点进行重组，使得具有基本功能或编码具有基本功能的产物的核酸变得不起作用，从而修饰宿主细胞目标位点。本发明还涉及通过本发明的方法获得的细胞。

28. 发明公开

EP2831238A1 CLONING METHOD 有权
标题翻译： KLONVERFAHREN
公开(公告)号：EP2831238A1
公开(公告)日：2015-02-04
申请号：EP13716982.7
申请日：2013-03-27
申请人： DSM IP Assets B.V.
发明人： ROUBOS, Johannes, Andries , PEL, Herman Jan , MEIJRINK, Bernard
IPC分类号： C12N15/66 , C12N15/90
CPC分类号： C12N15/1024 , C12N15/66 , C12N15/90 , C12N2800/30 , C12N2800/40 , C12N2800/50 , C12N2800/80
摘要： The present invention relates to a method based on the use of restriction enzyme digestion and ligation via cleavage sites, thereby to prepare two or more standardized expression cassettes.
摘要翻译：本发明涉及一种基于限制酶消化和通过切割位点连接的方法，从而制备两个或多个标准化表达盒。

29. 发明公开

EP2825650A1 RECOMBINATION SYSTEM 有权
标题翻译：重组
公开(公告)号：EP2825650A1
公开(公告)日：2015-01-21
申请号：EP13708506.4
申请日：2013-03-12
申请人： DSM IP Assets B.V.
发明人： WIESSENHAAN, Nathalie , KOLEN, Catharina Petronella Antonia Maria , MEIJRINK, Bernard , BOER, Viktor , ROUBOS, Johannes, Andries , ARENDSEN, Yvonne Johannes Odilia
IPC分类号： C12N15/63 , C12N15/90 , C12N15/00 , C12N5/00
CPC分类号： C12N15/102 , C12N15/902 , C12N2800/30
摘要： The present invention relates to a method for carrying out recombination at a target locus.

30. 发明公开

EP2783000A1 NUCLEIC ACID ASSEMBLY SYSTEM 审中-公开
标题翻译：系统对于核酸安排
公开(公告)号：EP2783000A1
公开(公告)日：2014-10-01
申请号：EP12788597.8
申请日：2012-11-23
申请人： DSM IP Assets B.V.
发明人： ROUBOS, Johannes, Andries , MEIJRINK, Bernard , KERKMAN, Richard , DULK, DEN, Ben
IPC分类号： C12N15/10 , C12N15/52 , C40B40/06 , C40B40/08
CPC分类号： C12N15/1082 , C12N15/1027 , C12N15/1093 , C12N15/52 , C12N15/80 , C12N15/905
摘要： The present invention relates to a method for the preparation of a library of host cells, a plurality of which comprise an assembled polynucleotide at a target locus, which method comprises: (a) providing a plurality of polynucleotides comprising two or more polynucleotide subgroups, wherein: (i) a plurality of polynucleotides in each polynucleotide subgroup comprises sequence encoding a peptide or polypeptide and/or a regulatory sequence; (ii) a plurality of peptides or polypeptides encoded by, or a plurality of regulatory sequences comprised within, each polynucleotide subgroup share an activity and/or function; (iii) at least one polynucleotide subgroup comprises at least two non-identical polynucleotide species; (iv) a plurality of polynucleotides of each polynucleotide subgroup comprises sequence enabling homologous recombination with a plurality of polynucleotides from one or more other polynucleotide subgroups; and (v) a plurality of polynucleotides in two polynucleotide subgroups comprise a nucleotide sequence enabling homologous recombination with a target locus in host cells; and (b) assembling the plurality of polynucleotides at the target locus by homologous recombination in vivo in host cells, thereby to generate a library of host cells, a plurality of which comprise an assembled polynucleotide at the target locus. The assembled polynucleotides may be recovered, thereby to prepare a library of nucleic acids.

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