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21. 发明授权

US5994508A Isoflavone rich protein isolate and process for producing 失效
标题翻译：富含异黄酮的蛋白质分离和生产过程
公开(公告)号：US5994508A
公开(公告)日：1999-11-30
申请号：US59513
申请日：1998-04-13
申请人： Barbara A. Bryan , Balagtas F. Guevara
发明人： Barbara A. Bryan , Balagtas F. Guevara
IPC分类号： A23J1/14 , A23J3/16 , C07D311/36 , C07H15/26 , A23L2/38
CPC分类号： A23J3/16 , C07D311/36 , C07H15/26
摘要： A process for providing an isoflavone rich protein isolate is provided, along with the isoflavone rich protein isolate produced thereby. A vegetable material containing protein and at least one isoflavone compound is extracted with an aqueous extractant having a neutral pH. The protein and isoflavones are extracted into the extractant, and the extractant containing the protein and isoflavones is separated from insoluble vegetable materials to form a protein extract. The pH of the protein extract is adjusted to about the isoelectric point of the protein to precipitate the protein. The extract containing the precipitated protein is cooled to a temperature of from about 40.degree. F. to about 80.degree. F., and then the protein is separated from the extract. The cool separation temperatures unexpectedly significantly increase the concentration of isoflavones recovered in the separated protein, while the neutral extract pH inhibits loss of protein normally observed at cool or cold separation temperatures.
摘要翻译：提供了富含异黄酮的蛋白质分离物的方法，以及由此产生的富含异黄酮的蛋白质分离物。含有蛋白质和至少一种异黄酮化合物的植物材料用具有中性pH的水性萃取剂萃取。将蛋白质和异黄酮提取到提取剂中，将含有蛋白质和异黄酮的提取物从不溶性植物材料中分离以形成蛋白质提取物。将蛋白质提取物的pH调节至蛋白质的等电点以沉淀蛋白质。将含有沉淀蛋白质的提取物冷却至约40°F至约80°F的温度，然后将蛋白质与提取物分离。冷分离温度意外地显着增加分离的蛋白质中回收的异黄酮的浓度，而中性提取物pH抑制在冷或分离温度下通常观察到的蛋白质的损失。

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