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    • 21. 发明授权
    • Nucleotide sequences which code for the menE gene
    • 编码menE基因的核苷酸序列
    • US06946271B2
    • 2005-09-20
    • US09834722
    • 2001-04-16
    • Mike FarwickKlaus HuthmacherAchim MarxWalter Pfefferle
    • Mike FarwickKlaus HuthmacherAchim MarxWalter Pfefferle
    • C12N9/00C12P13/08
    • C12N9/93C12P13/08C12Y602/01026
    • The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the menE gene is present in attenuated form, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
    • 本发明涉及一种分离的多核苷酸,其包含多核苷酸序列,所述多核苷酸序列选自a)与编码多肽的多核苷酸的程度相同的多核苷酸,所述多核苷酸包含SEQ ID NO: 2,b)编码多肽的多核苷酸,其包含与至少70%与SEQ ID No.2的氨基酸序列相同程度的氨基酸序列,c)多核苷酸,其与 )或b),和d)包含a),b)或c)的多核苷酸序列的至少15个连续核苷酸的多核苷酸,以及使用棒状细菌发酵制备L-氨基酸的方法,其中至少所述menE 基因以减毒形式存在,并且使用包含根据本发明的序列的多核苷酸作为杂交探针。
    • 29. 发明授权
    • Nucleotide sequences coding for the genes sucC and sucD
    • 编码基因sucC和sucD的核苷酸序列
    • US06897055B2
    • 2005-05-24
    • US09838564
    • 2001-04-20
    • Bettina MöckelWalter PfefferleAchim Marx
    • Bettina MöckelWalter PfefferleAchim Marx
    • C12N9/00C12P13/04C12P13/14C12N9/12
    • C12N9/93C12P13/04C12P13/14
    • The invention relates to polynucleotides that contain polynucleotide sequences coding for the genes sucC and sucD, selected from the group a) polynucleotide that is at least 70% identical to a polynucleotide coding for a polypeptide that contains the amino acid sequence of SEQ ID No. 2, b) polynucleotide that is at least 70% identical to a polynucleotide coding for a polypeptide that contains the amino acid sequence of SEQ ID No. 3, c) polynucleotide coding for a polypeptide that contains an amino acid sequence that is at least 70% identical to the amino acid sequence of SEQ ID No. 2, d) polynucleotide coding for a polypeptide that contains an amino acid sequence that is at least 70% identical to the amino acid sequence of SEQ ID No. 3, e) polynucleotide that is complementary to the polynucleotides of a), b), c) or d), and f) polynucleotide containing at least 15 successive nucleotides of the polynucleotide sequence of a), b), c), d) or e), a process for the fermentative production of L-amino acids using coryneform bacteria in which the genes are present in attenuated form, and the use of the polynucleotide sequences as hybridization probes.
    • 本发明涉及含有编码基因sucC和sucD的多核苷酸序列的多核苷酸,其选自a)与编码含有SEQ ID No.2的氨基酸序列的多肽的多核苷酸至少70%相同的多核苷酸 ,b)与编码含有SEQ ID No.3的氨基酸序列的多肽的多核苷酸至少70%相同的多核苷酸,c)编码含有至少70%的氨基酸序列的多肽的多核苷酸, 与SEQ ID No.2的氨基酸序列相同,d)编码含有与SEQ ID No.3的氨基酸序列至少70%相同的氨基酸序列的多肽的多核苷酸,e) 与a),b),c)或d)的多核苷酸互补,以及f)包含a),b),c),d)或e)的多核苷酸序列的至少15个连续核苷酸的多核苷酸, 发酵生产 使用棒状细菌的L-氨基酸,其中基因以减毒形式存在,以及使用多核苷酸序列作为杂交探针。