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21. 发明申请

WO03045310A2 DIGITAL ASSAY 审中-公开
标题翻译：数字测定
公开(公告)号：WO03045310A2
公开(公告)日：2003-06-05
申请号：PCT/US0237499
申请日：2002-11-21
申请人： APPLERA CORP , VANN CHARLES S , LAO KAI QIN , REED MARK
发明人： VANN CHARLES S , LAO KAI QIN , REED MARK
IPC分类号： G01N33/53 , A61K20060101 , C07H21/02 , C12N9/22 , C12P19/34 , C12Q1/68 , G01N33/543 , G01N33/566 , A61K
CPC分类号： C12Q1/6816 , B82Y5/00 , B82Y10/00 , C12Q1/6827 , C12Q2563/155 , C12Q2561/125 , C12Q2545/114 , C12Q2565/102 , C12Q2563/143
摘要： The invention relates to methods and compositions for the detection of targets in a sample.
摘要翻译：本发明涉及用于检测样品中靶标的方法和组合物。

22. 发明公开

EP1924708A4 PURE MIRNA SAMPLE PREPARATION METHOD 审中-公开
标题翻译：过程对于纯的miRNA PROBE
公开(公告)号：EP1924708A4
公开(公告)日：2010-03-10
申请号：EP06787638
申请日：2006-07-17
申请人： APPLERA CORP
发明人： LAO KAI QIN
IPC分类号： C12Q1/68
CPC分类号： C12N15/1003 , C12N1/06 , C12N1/08 , C12N15/111 , C12N2310/14 , C12N2310/315 , C12N2310/321 , C12N2310/341 , C12N2310/346 , C12N2330/10 , C12N2330/30 , C12P19/34 , C12Q1/68 , C12N2310/3521

23. 发明公开

EP1910397A4 HOT START REVERSE TRANSCRIPTION BY PRIMER DESIGN 审中-公开
标题翻译： ENTWURF EINER HEISSSTART-RÜCKÜBERTRAGUNGDURCH PRIMER
公开(公告)号：EP1910397A4
公开(公告)日：2009-07-01
申请号：EP06800091
申请日：2006-07-17
申请人： APPLERA CORP
发明人： LAO KAI QIN , STRAUS NEIL , LIVAK KENNETH
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6876 , C07H21/02 , C07H21/04 , C12N9/00 , C12Q1/6844 , C12Q1/6848 , C12Q1/686 , C12Q2549/101 , C12Q2533/101 , C12Q2525/301
摘要： The present teachings provide methods, compositions, and kits for performing primer extension reactions. In some embodiments, a reverse transcription reaction is performed on a target polynucleotide with a hot start primer comprising a blunt-ended self-complementary stem, and a loop, and extension products form at high temperatures but reduce extension product formation at low temperatures.
摘要翻译：本教导提供了用于进行引物延伸反应的方法，组合物和试剂盒。在一些实施方案中，对靶多核苷酸进行逆转录反应，所述靶多核苷酸具有包含平末端自互补茎的热起始引物，并且在高温下形成环，并延伸产物在低温下形成延伸产物。

24. 发明公开

EP1917367A4 METHODS FOR RNA PROFILING 审中-公开
标题翻译：方法RNA配置文件创建
公开(公告)号：EP1917367A4
公开(公告)日：2009-07-15
申请号：EP06800092
申请日：2006-07-17
申请人： APPLERA CORP
发明人： LIVAK KENNETH , LAO KAI QIN
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6883 , C12Q1/6809 , C12Q1/6876 , C12Q2600/158 , C12Q2600/178 , C12Q2525/207
摘要： The present teachings provide methods, compositions, and kits for detecting micro RNAs (miRNAs). In some embodiments, the miRNAs are quantified from formalin-fixed paraffin-embedded tissue samples in which messenger RNA is degraded. The present teachings take advantage of the observation that most mature miRNAs in vivo are protected by degradation as a result of their association with RISC. Thus, novel methods of studying nucleic acids in archived tissues containing degraded messenger RNA are provided, wherein RISC-protected miRNAs are liberated, and analyzed.

25. 发明公开

EP1910549A4 ANALYZING MESSENGER RNA AND MICRO RNA IN THE SAME REACTION MIXTURE 有权
标题翻译：信使RNA和微RNA在同一反应路径分析
公开(公告)号：EP1910549A4
公开(公告)日：2009-03-25
申请号：EP06787639
申请日：2006-07-17
申请人： APPLERA CORP
发明人： LAO KAI QIN , STRAUS NEIL A
IPC分类号： C12P19/34 , C12Q1/68
CPC分类号： C12Q1/686 , C12Q1/6809 , C12Q1/6844 , C12Q1/6848 , C12Q1/6853 , C12Q2549/101 , C12Q2537/143 , C12Q2527/107 , C12Q2525/301 , C12Q2525/207 , C12Q2533/101
摘要： The present teachings provide methods, compositions, and kits for performing primer extension reactions on at least two target polynucleotides in the same reaction mixture. In some embodiments, a reverse transcription reaction is performed on a first target polynucleotide with a hot start primer comprising a self-complementary stem and a loop, and extension products form at high temperatures but extension products form less so at low temperatures since the self-complementary stem of the hot start primer prevents hybridization of the target specific region to the target. However, non-hot start primers with free target specific regions can hybridize to their corresponding targets at the low temperature and extension can happen at the low temperature.

26. 发明申请

WO2007032817A3 PURE MIRNA SAMPLE PREPARATION METHOD 审中-公开
标题翻译：纯MIRNA样品制备方法
公开(公告)号：WO2007032817A3
公开(公告)日：2009-05-07
申请号：PCT/US2006027756
申请日：2006-07-17
申请人： APPLERA CORP , LAO KAI QIN
发明人： LAO KAI QIN
IPC分类号： C12Q1/68
CPC分类号： C12N15/1003 , C12N1/06 , C12N1/08 , C12N15/111 , C12N2310/14 , C12N2310/315 , C12N2310/321 , C12N2310/341 , C12N2310/346 , C12N2330/10 , C12N2330/30 , C12P19/34 , C12Q1/68 , C12N2310/3521
摘要： The present teachings provide novel methods, compositions, and kits for analyzing mature micro RNAs (miRNAs). By taking advantage of the observation that most mature miRNAs in cells are tightly associated with RISCs, the present teachings provide approaches for studying mature miRNAs without the complications of additional nucleic acids. For example, in some embodiments the present teachings provide a method of purifying mature miRNAs comprising heating a sample to form a lysate, and, degrading the additional nucleic acids. The resulting mixture lacks the additional nucleic acids, and contains mature miRNAs associated with RISCs. Liberating the mature miRNAs from RISCs, for example by a protease, a detergent, and/or heat, can result in a pure collection of mature miRNAs.
摘要翻译：本教导提供用于分析成熟微RNA（miRNA）的新型方法，组合物和试剂盒。通过利用细胞中大多数成熟miRNA与RISC紧密相关的观察，本教导提供了研究成熟miRNA而没有附加核酸并发症的方法。例如，在一些实施方案中，本发明提供纯化成熟miRNA的方法，其包括加热样品以形成裂解物，以及降解另外的核酸。所得混合物缺乏额外的核酸，并且含有与RISC相关的成熟miRNA。从RISC解离成熟的miRNA，例如通过蛋白酶，洗涤剂和/或加热，可导致成熟miRNA的纯收集。

27. 发明申请

WO2006115570A3 SMALL NUCLEIC ACID DETECTION PROBES AND USES THEREOF 审中-公开
标题翻译：小核酸检测探针及其用途
公开(公告)号：WO2006115570A3
公开(公告)日：2007-11-01
申请号：PCT/US2006005828
申请日：2006-02-17
申请人： APPLERA CORP , LAO KAI QIN , STRAUS NEIL A
发明人： LAO KAI QIN , STRAUS NEIL A
IPC分类号： C12Q1/68 , C07H21/02
CPC分类号： C12Q1/6818 , C12Q2563/173 , C12Q2545/114 , C12Q2525/301
摘要： The present teachings are directed to compositions, methods, and kits for detecting and quantitating small nucleic acid molecules, including small DNA molecules and small RNA molecules. The detector probes of the current teachings, including single-loop detector probes, double-loop detector probes, and bimolecular detector probes, are designed to selectively hybridize with a corresponding small nucleic acid target and to produce, under appropriate conditions, a detectable signal or a detectably different signal. The detector complexes of the current teachings comprise a detector probe comprising a first reporter group and a displaceable sequence comprising a second reporter group, wherein the displaceable sequence is hybridized to the detector probe. According to certain methods, detecting a small nucleic acid target comprises the target displacing the displaceable sequence of a detector complex to form a detector probe-small nucleic acid target duplex, illuminating the duplex with light of an appropriate wavelength, and determining the presence of a detectable fluorescent signal or the change in a detectable signal.
摘要翻译：本教导涉及用于检测和定量小核酸分子（包括小DNA分子和小RNA分子）的组合物，方法和试剂盒。目前的教导的检测器探针，包括单环检测器探针，双环检测器探针和双分子检测器探针被设计为与相应的小核酸靶选择性杂交，并在适当条件下产生可检测信号或一个可检测的不同信号。本教导的检测器复合物包括检测器探针，其包括第一报告基团和包含第二报告基团的可置换序列，其中可移位序列与检测器探针杂交。根据某些方法，检测小核酸靶包括靶取代检测器复合物的可置换序列以形成检测器探针 - 小核酸靶双链体，用合适波长的光照亮双链体，并确定可检测的荧光信号或可检测信号的变化。

28. 发明申请

WO2007011902A2 METHODS FOR RNA PROFILING 审中-公开
标题翻译： RNA分析方法
公开(公告)号：WO2007011902A2
公开(公告)日：2007-01-25
申请号：PCT/US2006027754
申请日：2006-07-17
申请人： APPLERA CORP , LAO KAI QIN , LIVAK KENNETH
发明人： LAO KAI QIN , LIVAK KENNETH
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6883 , C12Q1/6809 , C12Q1/6876 , C12Q2600/158 , C12Q2600/178 , C12Q2525/207
摘要： The present teachings provide methods, compositions, and kits for detecting micro RNAs (miRNAs). In some embodiments, the miRNAs are quantified from formalin-fixed paraffin-embedded tissue samples in which messenger RNA is degraded. The present teachings take advantage of the observation that most mature miRNAs in vivo are protected by degradation as a result of their association with RISC. Thus, novel methods of studying nucleic acids in archived tissues containing degraded messenger RNA are provided, wherein RISC-protected miRNAs are liberated, and analyzed.
摘要翻译：本教导提供了用于检测微RNA（miRNA）的方法，组合物和试剂盒。在一些实施方案中，从其中信使RNA降解的福尔马林固定的石蜡包埋的组织样品定量miRNA。本教导利用了这样的观察，即由于它们与RISC相关的结果，大多数成熟的miRNA在体内被降解保护。因此，提供了研究含有降解信使RNA的存档组织中的核酸的新方法，其中释放RISC保护的miRNA并进行分析。

29. 发明申请

WO2006005081A3 COMPOSITIONS AND METHODS FOR IDENTIFYING NUCLEOTIDES IN POLYNUCLEOTIDE SEQUENCES 审中-公开
标题翻译：用于鉴定多核苷酸序列中的核苷酸的组合物和方法
公开(公告)号：WO2006005081A3
公开(公告)日：2006-11-16
申请号：PCT/US2005023949
申请日：2005-06-30
申请人： APPLERA CORP , LAO KAI QIN
发明人： LAO KAI QIN
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6858 , C12Q1/6823 , C12Q2525/161 , C12Q2521/101 , C12Q2563/179 , C12Q2521/319
摘要： The present disclosure provides methods and compositions to detect a specific nucleotide at a selected position of a polynucleotide target sequence.
摘要翻译：本公开提供了检测多核苷酸靶序列的选定位置处的特定核苷酸的方法和组合物。

30. 发明申请

WO2006065597A3 ID-TAG COMPLEXES, ARRAYS, AND METHODS OF USE THEREOF 审中-公开
标题翻译： ID-TAG复合物，阵列及其使用方法
公开(公告)号：WO2006065597A3
公开(公告)日：2006-07-27
申请号：PCT/US2005044269
申请日：2005-12-06
申请人： APPLERA CORP , SCHROTH GARY P , LAO KAI QIN , STRAUS NEIL A
发明人： SCHROTH GARY P , LAO KAI QIN , STRAUS NEIL A
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6823 , C12Q1/6816 , C12Q2565/519 , C12Q2537/1373 , C12Q2537/125 , C12Q2565/501 , C12Q2563/131 , C12Q2525/197 , C12Q2563/185
摘要： The present invention relates to the detection of target sequences. Detection can be achieved through the use of ID-tag complexes. These ID-tag complexes are relatively stable in the absence of a target sequence. In the presence of a target sequence, the complexes dissociate and form new complexes or duplexes, which can be purified or eliminated and detected on an ID-tag system.
摘要翻译：本发明涉及靶序列的检测。检测可以通过使用ID-标签复合物来实现。这些ID-标签复合物在不存在靶序列的情况下相对稳定。在靶序列的存在下，复合物解离并形成新的复合物或双链体，其可以在ID-标签系统上纯化或消除并检测。

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