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    • 29. 发明公开
    • 바실러스 폴리퍼멘티쿠스 케이제이에스-2 균주가 생산하는항균물질 마크로락틴 에이
    • 抗生素大豆蛋白A生产的BACILLUS POLYFERMENKUS KJS-2
    • KR1020080084479A
    • 2008-09-19
    • KR1020070026285
    • 2007-03-16
    • 일동홀딩스(주)인제대학교 산학협력단강재선
    • 강재선김천규김동희김강민김동훈이진영최광진차인준홍재선홍용근
    • C12P17/02C12P17/08
    • C12P17/08C07D313/00C12R1/07
    • An antibacterial macrolactin A produced from Bacillus polyfermenticus KJS-2 is provided to improve range of antibacterial spectrum against various microorganisms and fungi, especially vancomycin-resistant Enterococci(VRE) and methicillin-resistant Staphylococcus aureus(MRSA). An antibacterial macrolactin A produced from Bacillus polyfermenticus KJS-2(KCCM 10769P) has MIC(minimum inhibitory concentration)>90 of 15.63-31.25 mug/ml and 7.81-31.25 mug/ml against vancomycin-resistant Enterococci(VRE) and methicillin-resistant Staphylococcus aureus(MRSA), respectively, and is isolated by culturing Bacillus polyfermenticus KJS-2(KCCM 10769P) in TSB(tryptone soy broth) agar medium, extracting the cultured medium with ethyl acetate, subjecting the extract to LC(liquid chromatography)/Mass to obtain a fraction showing improved antibacterial activity, and purifying the fraction with preparative silica gel RP-18.
    • 提供了一种由芽孢杆菌KJS-2生产的抗菌性大黄精乳素A,以改善各种微生物和真菌,特别是万古霉素耐药性肠球菌(VRE)和耐甲氧西林金黄色葡萄球菌(MRSA)的抗菌谱范围。 由芽孢杆菌KJS-2(KCCM 10769P)产生的抗菌性大多数内生素A具有对万古霉素耐药性肠球菌(VRE)和耐甲氧西林抗性的15.63-31.25μg/ ml和7.81-31.25μg/ ml的MIC(最小抑制浓度)> 90 通过在TSB(胰蛋白胨大豆肉汤)琼脂培养基中培养芽胞杆菌KJS-2(KCCM 10769P),用乙酸乙酯萃取培养的培养基,将提取物进行LC(液相色谱)/质谱分析,分离出金黄色葡萄球菌(MRSA) 质量以获得显示出改善的抗菌活性的级分,并用制备型硅胶RP-18纯化该级分。