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21. 发明专利

JP2005514072A High fidelity dna polymerase compositions and the use thereof 有权
公开(公告)号：JP2005514072A
公开(公告)日：2005-05-19
申请号：JP2003560226
申请日：2002-12-17
申请人：ストラタジーンカリフォルニアStratagene California
发明人：ジョセフエイ．ソージ、 , マイケルボーンズ、 , ホリーホグレフ、
IPC分类号： C12N15/09 , C12N9/10 , C12N9/12 , C12Q1/68
CPC分类号： C12N9/1252
摘要：本発明は、第１酵素および第２酵素を含む酵素混合物を含む組成物に関連する。ここで第１酵素はＤＮＡ重合活性を備え、そして第２酵素は３'−５'エキソヌクレアーゼ活性および低下したＤＮＡ重合活性を備える。本発明はさらに、本発明の組成物を使用する高忠実度ＤＮＡ合成のためのキット構成内の上記組成物および方法に関する。

22. 发明申请

US20040241861A1 Highly transformable bacterial cells and methods for producing the same 失效
公开(公告)号：US20040241861A1
公开(公告)日：2004-12-02
申请号：US10800389
申请日：2004-03-12
申请人： Stratagene
发明人： Alan Lewis Greener , Bruce Douglas Jerpseth
IPC分类号： C12N001/21 , C12N015/74
CPC分类号： C12R1/19 , C12N15/70 , C12N15/74
摘要： The invention provided herein includes novel gram negative bacteria cells containing the Hte mutation. Other aspects of the invention include methods for rendering gram negative bacterial cells bearing the Hte region, such as E. coli cells competent for DNA transformation using any of a variety of competency inducing procedures. The competent cells of the subject invention may be frozen so as to provide for prolonged storage.

23. 发明申请

US20040175704A1 Compositions and methods for polynucleotide sequence detection 审中-公开
标题翻译：多核苷酸序列检测的组合物和方法
公开(公告)号：US20040175704A1
公开(公告)日：2004-09-09
申请号：US10436231
申请日：2003-05-12
申请人： Stratagene
发明人： Joseph A. Sorge , Andrew Firmin
IPC分类号： C12Q001/68
CPC分类号： C12Q1/6818 , B82Y5/00 , B82Y10/00 , C12Q1/6827 , C12Q2565/1015 , C12Q2535/125
摘要： The present invention provides compositions, kits, and methods for detecting polynucleotide sequence differences. The method involves amplifying a polynucleotide in the presence of a labeled nucleotide whose incorporation into the amplified product can indicate the presence of a sequence difference within the polynucleotide template. The invention is particularly useful for differentiating two or more closely related polynucleotide sequences, for example, in determining which allele or alleles of a multiallelic organism are present in a target polynucleotide.
摘要翻译：本发明提供了用于检测多核苷酸序列差异的组合物，试剂盒和方法。该方法包括在标记核苷酸存在下扩增多核苷酸，其掺入到扩增产物中可以指示在多核苷酸模板内存在序列差异。本发明特别可用于区分两个或更多个密切相关的多核苷酸序列，例如在确定多核生物的等位基因或等位基因存在于靶多核苷酸中。

24. 发明申请

US20040149725A1 Apparatus and method for thermally cycling samples of biological material with substantial temperature uniformity 有权
标题翻译：用于热循环具有显着温度均匀性的生物材料样品的装置和方法
公开(公告)号：US20040149725A1
公开(公告)日：2004-08-05
申请号：US10691874
申请日：2003-10-23
申请人： Stratagene
发明人： Larry Richard Brown
IPC分类号： H05B003/02
CPC分类号： B01L7/52 , B01L7/54 , B01L2200/147 , B01L2300/0829 , B01L2300/1805
摘要： An apparatus for thermally cycling samples of a biological material including a thermal block assembly including a plurality of sample holders for receiving samples of biological material; a heat sink thermally coupled to the thermal block assembly, the heat sink transferring heat away from the thermal block assembly to ambient air in contact with the heat sink; a first heat source thermally coupled to the thermal block assembly to provide heat to the thermal block assembly; and a second heat source thermally coupled to the first heat source and configured to provide heat to a portion of the first heat source. The arrangement of the heat sink, first heat source and second heat source can provide substantial temperature uniformity among the plurality of sample holders. The invention also includes a method for thermally cycling samples of biological material.
摘要翻译：一种用于热循环生物材料样品的装置，包括包含多个用于接收生物材料样品的样品保持器的热块组件; 热耦合到热块组件的散热器，散热器将热量从热块组件传递到与散热器接触的环境空气中; 热耦合到所述热块组件以向所述热块组件提供热量的第一热源; 以及第二热源，其热耦合到所述第一热源并且被配置为向所述第一热源的一部分提供热量。散热器，第一热源和第二热源的布置可以在多个样品保持器之间提供显着的温度均匀性。本发明还包括用于热循环生物材料样品的方法。

25. 发明申请

US20040086890A1 DNA polymerases with reduced base analog detection activity 审中-公开
公开(公告)号：US20040086890A1
公开(公告)日：2004-05-06
申请号：US10408601
申请日：2003-04-07
申请人： Stratagene
发明人： Joseph A. Sorge , Holly H. Hogrefe , Madhushree Ghosh
IPC分类号： C12Q001/68 , C07H021/04 , C12P019/34 , C12N009/22
CPC分类号： C12N9/1252 , C07H21/04 , C12Q1/6869 , C12Q2535/101 , C12Q2521/101
摘要： The invention relates to the generation and characterization of archaeal DNA polymerase mutants with reduced base analog detection activity. The invention further provides for archaeal DNA polymerase mutants with reduced base analog detection activity containing additional mutations that modulate other DNA polymerase activities including DNA polymerization or 3null-5null exonuclease activity. The invention also discloses methods and applications of DNA polymerases with reduced base analog detection activity.

26. 发明申请

US20030088109A1 Fluorescent dye 有权
标题翻译：荧光染料
公开(公告)号：US20030088109A1
公开(公告)日：2003-05-08
申请号：US10087072
申请日：2002-02-28
申请人： Stratagene
发明人： Jack Anderson , Jeffrey Carl Braman
IPC分类号： C07D513/22 , C07D487/02
CPC分类号： C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C09B23/0075 , C09B23/06 , C12Q1/6837 , Y10S436/80 , Y10T436/143333 , C12Q2563/107
摘要： The invention relates to fluorescent dyes. More particularly, the invention relates to fluorescent cyanine dyes, and especially to water soluble fluorescent cyanine dyes that contain additional sites for attachment to biomolecules. The invention provides a group of novel, water soluble fluorescent cyanine dyes that have distinct fluorescence characteristics that permit their use in any assay or method suited to water soluble fluorescent dyes, and especially to assays requiring a plurality of distinguishable fluorescent markers. The invention further relates to nucleotides, nucleosides, polynucleotides and polypeptides labeled with novel fluorescent cyanine dyes according to the invention, and methods of using them.
摘要翻译：本发明涉及荧光染料。更具体地说，本发明涉及荧光花青染料，尤其涉及含有附着于生物分子附加位点的水溶性荧光花青染料。本发明提供了一组具有不同荧光特性的新型水溶性荧光花青染料，其允许它们在适用于水溶性荧光染料的任何测定或方法中使用，特别是需要多个可区分荧光标记的测定法。本发明还涉及用根据本发明的新型荧光花青染料标记的核苷酸，核苷，多核苷酸和多肽，以及使用它们的方法。

27. 发明申请

US20010031483A1 Primer-mediated polynucleotide synthesis and manipulation techniques 审中-公开
标题翻译：引物介导的多核苷酸合成和操作技术
公开(公告)号：US20010031483A1
公开(公告)日：2001-10-18
申请号：US09795965
申请日：2001-02-28
申请人： Stratagene
发明人： Joseph A. Sorge , Kerstien A. Padgett
IPC分类号： C12P001/00 , C07K001/00 , C07K014/00 , C07K017/00 , C12Q001/68
CPC分类号： C12N15/66 , C12N15/10 , C12Q1/686 , C12Q2525/131 , C12Q2525/117
摘要： The invention provides improved techniques for conveniently manipulating polynucleotides of interest without the need to rely upon the presence of naturally occurring restriction sites. Additionally, using the methods and primers of the invention, one may synthesize a polynucleotide of interest in a form which is easily and directionally cloned into a DNA sequence of choice without necessarily introducing extraneous nucleotides in the final polynucleotide product. The methods of the invention employ releasable primers that comprise a recognition site for a releasing enzyme joined to a region for annealing to the polynucleotide template of interest. Polynucleotide sequences of interest are synthesized using one or more synthesis primers, wherein at least one of the primers is a releasable primer. After synthesis, the synthesis product is cleaved by a releasing enzyme. In a preferred embodiment of the invention, inhibitory base analogs are incorporated in the synthesis product to protect against the formation of unwanted internal cleavage products. In another embodiment of the invention, at least one of the releasable primers is bound to an immobilizing solid phase support so as to produce immobilized synthesis products that may be conveniently released by a releasing enzyme. Another aspect of the invention is to provide releasable primers and kits for performing the subject methods. Typically, such kits may comprise a releasing enzyme and one or more reagents for performing a polynucleotide synthesis reaction, preferably a cyclic amplification reaction.
摘要翻译：本发明提供了用于方便地操作感兴趣的多核苷酸的改进的技术，而不需要依赖天然存在的限制性位点的存在。另外，使用本发明的方法和引物，人们可以以容易且定向克隆到选择的DNA序列中的形式合成感兴趣的多核苷酸，而不必在最终多核苷酸产物中引入外来核苷酸。本发明的方法使用可释放的引物，其包含连接到用于退火的感兴趣的多核苷酸模板的区域的释放酶的识别位点。使用一种或多种合成引物合成感兴趣的多核苷酸序列，其中至少一种引物是可释放引物。合成后，合成产物被释放酶切割。在本发明的优选实施方案中，将抑制性碱基类似物掺入合成产物中以防止形成不需要的内部裂解产物。在本发明的另一个实施方案中，至少一个可释放的引物与固定的固相载体结合，以产生可通过释放酶方便地释放的固定的合成产物。本发明的另一方面是提供用于实施本发明方法的可释放的底漆和试剂盒。通常，这样的试剂盒可以包含释放酶和用于进行多核苷酸合成反应的一种或多种试剂，优选循环扩增反应。

28. 发明专利

JP4960560B2 Detection of a target nucleic acid sequence 有权
公开(公告)号：JP4960560B2
公开(公告)日：2012-06-27
申请号：JP2001535602
申请日：2000-10-27
申请人：ストラタジーンカリフォルニアStratagene California
发明人：ジョゼフエイ．ソーグ、
IPC分类号： C12N15/09 , C12N9/12 , C12N9/22 , C12Q1/68
CPC分类号： C12Q1/686 , C12Q1/6823 , Y10S435/81 , Y10S435/822 , Y10T436/143333 , C12Q2561/109 , C12Q2531/113

29. 发明专利

JP2010514453A The compositions and methods of using the divided polymerase 有权
公开(公告)号：JP2010514453A
公开(公告)日：2010-05-06
申请号：JP2009544326
申请日：2007-12-31
申请人：ストラタジーンカリフォルニアStratagene California
发明人：リディアウー、 , コニージェイ．ハンセン、 , ジェフェリーディ．フォックス、 , ホーリーエイチ．ホグリーフ、
IPC分类号： C12N15/09 , C12N9/12 , C12Q1/68
CPC分类号： C12N9/1252
摘要： The invention relates to compositions and methods utilizing split polymerase enzymes composed of at least two discrete polypeptides that stably associate to form a single polymerase. The invention further relates to nucleic acid constructs for expressing the split polymerases of the invention, and methods for using the split polymerases of the invention. The enzymes of the invention are useful in many applications calling for the detectable labeling of nucleic acids and are particularly useful in quantitative PCR (QPCR) and DNA sequencing applications.

30. 发明专利

JP2009504162A Mutant reverse transcriptase and method of use 有权
公开(公告)号：JP2009504162A
公开(公告)日：2009-02-05
申请号：JP2008526271
申请日：2006-08-10
申请人：ストラタジーンカリフォルニアStratagene California
发明人：アレジ，バーラム , シン，ウェイメイ , ホグリフ，ホリー
IPC分类号： C12N15/09 , C12M1/00 , C12N9/12 , C12N9/98 , C40B40/08
CPC分类号： C12Y207/07049 , C12N9/1276
摘要：本発明は、安定なＭＭＬＶ逆転写酵素変異体の生成および特徴に関する。本発明はまた、ＭＭＬＶ逆転写酵素変異体を用いる方法を開示する。

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